This paper presents the results of molecular studies on testing and evaluation of multiplex test systems for the genetically modified organisms’ (GMOs) identification by PCR in real time. For the detection of genetic modifications in plants of the cabbage family (Brassicaceae) screening of major regulatory sequences is insufficient, as in genetic engineering manipulations 35S promoter – of DNA-containing cauliflower mosaic virus (CaMV) is used, which presence in the test material can lead to false positive results. In this work the feasibility of testing for the presence of viral CaMV’s DNA and analysis of screened gene sequences of interest for the usage during the analysis of rapeseed (Brassica napus L.) samples. It was established that the analysis of the presence/absence of a positive result for the CaMV 35S promoter is not giving complete answers to the absence of GMO in the sample. The appropriateness of GMO’s screening in rape seeds with NOS terminator and genes of interest.

This paper presents the results of molecular studies on testing and evaluation of multiplex test systems for the genetically modified organisms’ (GMOs) identification by PCR in real time. For the detection of genetic modifications in plants of the cabbage family (Brassicaceae) screening of major regulatory sequences is insufficient, as in genetic engineering manipulations 35S promoter – of DNA-containing cauliflower mosaic virus (CaMV) is used, which presence in the test material can lead to false positive results. In this work the feasibility of testing for the presence of viral CaMV’s DNA and analysis of screened gene sequences of interest for the usage during the analysis of rapeseed (Brassica napus L.) samples. It was established that the analysis of the presence/absence of a positive result for the CaMV 35S promoter is not giving complete answers to the absence of GMO in the sample. The appropriateness of GMO’s screening in rape seeds with NOS terminator and genes of interest.

Assumption is made that identification of valuable genotypes is possible in specific stability points of phase-parametric systems, that is, when the effect of dynamism acquires gradient and discrete anisotropic composition by way of converting the phenotype variability in the temporal and parametric and characteristic specific vector-spaced system. Devices have been suggested (electronic board for the characteristics spectral expression, 3D configuration of a characteristic variation series) for displaying the location sample parametric pool in the general statistic range of quantitative characteristics. A characteristic has been detected (number of seeds in a pod) that carry the traits inherent in pseudo-qualitative and oligogenic characteristic. According to findings of the study, characteristics are identified that conform to various directions of economical use

Abstract Draft genomes of the fungal species Fusarium xylarioides, Teratosphaeria gauchensis and T. zuluensis are presented. In addition an annotation of the genome of Ceratocystis fimbriata is presented. Overall these genomes provide a valuable resource for understanding the molecular processes underlying pathogenicity and potential management strategies of these economically important fungi.

The results of evaluation and selection of breeding material of mixed germ-plasm corn for early ripeness among self-pollinated families S4 by the length of the «sprouting–flowering of 50% of ears» period are given. A signifi cant infl uence of weather conditions during the years of study on the length of this indicator was determined. Response of studied corn test-crosses on growing conditions are described. Self-pollinated families DK22282111, DK28211111, DK21511111, DK20192321, DK26143111 were singled out which had a high tolerance to growing conditions and stable «sprouting–flowering of 50% of ears» period.

The results of evaluation and selection of breeding material of mixed germ-plasm corn for early ripeness among self-pollinated families S4 by the length of the «sprouting–flowering of 50% of ears» period are given. A signifi cant infl uence of weather conditions during the years of study on the length of this indicator was determined. Response of studied corn test-crosses on growing conditions are described. Self-pollinated families DK22282111, DK28211111, DK21511111, DK20192321, DK26143111 were singled out which had a high tolerance to growing conditions and stable «sprouting–flowering of 50% of ears» period.

The article quotes data on fruits biochemical composition for 101 varieties of peach from Nikita Botanical Garden collection. 88 varieties have been singled out according to the composition of bioactive substances. Among those varieties the North China and Iran ecological and geographical groups prevail (52.3% and 37.5%, respectively).

The article quotes data on fruits biochemical composition for 101 varieties of peach from Nikita Botanical Garden collection. 88 varieties have been singled out according to the composition of bioactive substances. Among those varieties the North China and Iran ecological and geographical groups prevail (52.3% and 37.5%, respectively).

У досліді вивчали формування селекційних індексів сортів пшениці озимої в різних умовах середовища в роки з ранньою та пізньою весняною вегетацією. Рання або пізня вегетація – це стресовий чинник для генотипу, і, безумовно, в технології селекційного процесу важливо знати, як впливають стресові чинники на формування та мінливість селекційних індексів. За результатами досліджень встановлено, що в стресових умовах середовища під час доборів на потенціал урожаю на ранніх етапах селекції можна використовувати такі селекційні індекси, як збиральний та індекс лінійної щільності колосу, які можуть бути використані як маркерні ознаки.

Homortomyces is introduced as a new coelomycetous genus associated with leaf spots on Combretum erythrophyllum trees growing near and around the Sterkfontein caves, Maropeng, South Africa. Based on its transversely septate, brown conidia, the presence of paraphyses, and percurrent proliferation of the conidiogenous cells, the genus resembles Stilbospora (Melanoconidaceae, Diaporthales). It is distinct in having pycnidial condiomata, conidia lacking mucoid sheaths, and becoming muriform when mature. Its morphology and phylogenetic placement based on analyses of sequence data for the large subunit nuclear ribosomal RNA gene (LSU, 28S) as well as the ITS and 5.8S rRNA gene of the nrDNA operon, show that Homortomyces represents a novel genus in Dothideomycetes, although its familial relationships remain unresolved.

Homortomyces is introduced as a new coelomycetous genus associated with leaf spots on Combretum erythrophyllum trees growing near and around the Sterkfontein caves, Maropeng, South Africa. Based on its transversely septate, brown conidia, the presence of paraphyses, and percurrent proliferation of the conidiogenous cells, the genus resembles Stilbospora (Melanoconidaceae, Diaporthales). It is distinct in having pycnidial condiomata, conidia lacking mucoid sheaths, and becoming muriform when mature. Its morphology and phylogenetic placement based on analyses of sequence data for the large subunit nuclear ribosomal RNA gene (LSU, 28S) as well as the ITS and 5.8S rRNA gene of the nrDNA operon, show that Homortomyces represents a novel genus in Dothideomycetes, although its familial relationships remain unresolved.

Homortomyces is introduced as a new coelomycetous genus associated with leaf spots on Combretum erythrophyllum trees growing near and around the Sterkfontein caves, Maropeng, South Africa. Based on its transversely septate, brown conidia, the presence of paraphyses, and percurrent proliferation of the conidiogenous cells, the genus resembles Stilbospora (Melanoconidaceae, Diaporthales). It is distinct in having pycnidial condiomata, conidia lacking mucoid sheaths, and becoming muriform when mature. Its morphology and phylogenetic placement based on analyses of sequence data for the large subunit nuclear ribosomal RNA gene (LSU, 28S) as well as the ITS and 5.8S rRNA gene of the nrDNA operon, show that Homortomyces represents a novel genus in Dothideomycetes, although its familial relationships remain unresolved.

The results obtained during the study of resistance of promising gooseberry varieties to Septoria leaf spot in the Western Forest-Steppe of Ukraine are described. Variety samples were divided into groups by the resistance to disease. Among them none was found with complete field  resistance. Sladkiy proved to be highly resistant variety. Resistance above the average is typical for Berill, Grushenka, Kooperator, Lehin, Malakhit, Rodnik, Shennon varieties. Hrossulyar, Kamenyar, Karat, Knyazhych, Kolobok, Krasnoslavyanskiy, Laskovyi, Mazershkota, Russkiy, Svityaz, Severnyi kapitan are varieties of average resistance to Septoria leaf spot. Zyelyonyi dozhd, Zlatogor, Invikta, Kubanyets, Nesluhivskyi, Slivovyi are characterized as low resistant ones.

The results obtained during the study of resistance of promising gooseberry varieties to Septoria leaf spot in the Western Forest-Steppe of Ukraine are described. Variety samples were divided into groups by the resistance to disease. Among them none was found with complete field resistance. Sladkiy proved to be highly resistant variety. Resistance above the average is typical for Berill, Grushenka, Kooperator, Lehin, Malakhit, Rodnik, Shennon varieties. Hrossulyar, Kamenyar, Karat, Knyazhych, Kolobok, Krasnoslavyanskiy, Laskovyi, Mazershkota, Russkiy, Svityaz, Severnyi kapitan are varieties of average resistance to Septoria leaf spot. Zyelyonyi dozhd, Zlatogor, Invikta, Kubanyets, Nesluhivskyi, Slivovyi are characterized as low resistant ones.

The research of 122 breeding lines of winter wheat to adaptability have been carried out for three years at three points. Samples for ecological variety testing were determined which have both high and average stability and plasticity, high average yield and are promising for qualified examination. The environments are analyzed for differentiating ability. Environments were selected which allow to identify genotypes for yield potential and adaptive ability. It is established that the parameters of adaptive ability and stability of winter wheat samples obtained during cultivation both in various geographical locations and at one point during some years were closely correlated. This confirms the hypothesis that the genotype estimate for adaptive ability in one location is reliable. The connection level was average only for the variance of genotype–environment interaction. However, this parameter is not principal therefore this connection level does not restrict significantly the use of the method in general.

The research of 122 breeding lines of winter wheat to adaptability have been carried out for three years at three points. Samples for ecological variety testing were determined which have both high and average stability and plasticity, high average yield and are promising for qualified examination. The environments are analyzed for differentiating ability. Environments were selected which allow to identify genotypes for yield potential and adaptive ability. It is established that the parameters of adaptive ability and stability of winter wheat samples obtained during cultivation both in various geographical locations and at one point during some years were closely correlated. This confirms the hypothesis that the genotype estimate for adaptive ability in one location is reliable. The connection level was average only for the variance of genotype–environment interaction. However, this parameter is not principal therefore this connection level does not restrict significantly the use of the method in general.

The author presents the study results concerning the infl uence of different systems of primary soil tillage on weed infestation on lands withdrawn from agriculture in Polissia of Ukraine. It is found that in case of returning such lands to use ploughing leads to depletion of weed seed stock in the layer of 0–10 cm by 36,1%. Surface and combined tillage systems help to reduce pollution of this soil layer with weed seeds by 25,4% and 31,7% respectively. When using surface tillage, a very shallow buriel of vegetative and generative organs of weeds that are close to the soil surface foster their early regrowth and accelerated development.

Various fungi were isolated during the course of a survey in a cold-store of apples in the Netherlands. One of these fungi belongs to the genus Penicillium and produces cleistothecia at 9 and 15 °C. A detailed study using a combination of phenotypic characters, sequences and extrolite patterns showed that these isolates belong to a new species within the series Roqueforti. The formation of cleistothecia at low temperatures and the inability to produce roquefortine C, together with a unique phylogenetic placement, make these isolates a novel entity in the Roqueforti series. The name Penicillium psychrosexualis sp. nov. (CBS 128137T) is proposed here for these isolates.