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Detoxification of aflatoxin B1 by Stenotrophomonas sp. CW117 and characterization the thermophilic degradation process
2020
Cai, Mengyu | Qian, Yingying | Chen, Nan | Ling, Tiejun | Wang, Jingjing | Jiang, Hong | Wang, Xu | Qi, Kezong | Zhou, Yu
Mycotoxins are high toxic, widely distributed contaminants in foodstuff. In this study, a aflatoxin B1 (AFB1) degrading strain S. acidoaminiphila CW117 was screened, and its detoxification characteristics were investigated. Substrate AFB1 at 45 μg/L was degraded by CW117 within 24 h; meanwhile, 4.1 mg/L AFB1 was almost degraded within 48 h. After 24 h degradation, the biotoxicity of the detoxified culture was eliminated. Strain CW117 efficient degradation to AFB1 (especially to low AFB1 concentrations) suggested its potential significance to detoxification development on food and feedstuff. The active degradation components present in the cell-free supernatant. The degradation ratio increased constantly with increasing incubation temperature raised (0–90 °C) and was even stable at 90 °C. Degradation was optimal at pH 6–7, and was only partially inhibited by metal-chelators (EDTA and EGTA), proteinase K, and a protein denaturant (sodium dodecyl sulfate, SDS). The recombinant laccase rLC1 (0.5 mg/mL) from CW117 degraded 29.3% of AFB1 within 24 h; however, the cell-free supernatant degraded 76.7% of the toxin in same time, with much lower protein content. The results indicated the CW117 degrades AFB1 via a combination of enzymes and micro-molecule oxides.
اظهر المزيد [+] اقل [-]Aflatoxicosis in cattle: clinical findings and biochemical alterations
2020
Elgioushy, Magdy M. | Elgaml, Shimaa A. | El-Adl, Mohamed M. | Hegazy, Ahmed Medhat | Hashish, Emad A.
Aflatoxicosis is a serious health condition resulted from aflatoxin (AF)-producing fungi. Major health threats resulted from AFs and reflect on the livestock industry with great economic losses. There are limited scientific evidences concerning the AFs in ruminant, therefore it is important to evaluate AFs health hazards in cattle. Here, we investigate biochemical, oxidative stress, and postmortem changes associated with unexpected acute bovine aflatoxicosis. Seventy-two cattle were suffered from aflatoxisocis. Depression and inappetence were predominant clinical findings of the diseased animals. Analysis of feedstuffs revealed presence of aflatoxin B1 (AFB1). The AF-intoxicated animals showed a significant increase in alanine amino transferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP), serum creatinine (SCr), catalase (CAT), and malondialdehyde (MDA) levels. Moreover, a significant decrease in total protein (TP), magnesium (Mg), and reduced glutathione (GSH) were also seen. Hepatomegaly, enlarged gallbladder as well as congestion of the intestine and kidney were observed. This study elucidates the critical and constructive measurements needed for the prevention of the AFs hazardous effects to livestock for the future control of AF outbreaks. Conducting series of diagnostic assays reflect the marked health condition alterations in the biochemical and antioxidant status of the AF-intoxicated cattle.
اظهر المزيد [+] اقل [-]Evaluation of acute and chronic exposure to aflatoxin B1 in indigenous women of the Huasteca Potosina, Mexico
2020
Díaz de León Martínez, Lorena | Rodríguez-Aguilar, Maribel | Wong-Arce, Alejandra | Díaz-Barriga, Fernando | Bañuelos Hernández, Bernardo | Rosales Mendoza, Sergio | Flores Ramírez, Rogelio
Aflatoxin B₁ (AFB₁) is one of the most studied mycotoxins due to its high occurrence in food and its hepatotoxic, immunosuppressive, carcinogenic, childhood growth, genotoxic, mutagenic, and teratogenic effects in humans and animals. Exposure to AFB₁ is reported to be both, acute and chronic; the main exposure pathway to AFB₁ is through the intake of contaminated food. In Mexico, although the reports of several studies addressing the problem of aflatoxins in maize and other foods, the evidence has been centered on exposure to AFB₁ and to the quantification of the Aflatoxins themselves, but there is null evidence about genotoxic effects of aflatoxins in vulnerable populations. Therefore, this study focused on assessing chronic AFB₁ exposure through the AFB₁-lys biomarker adduct and acute exposure through total AFB₁-DNA adducts in women from a rural indigenous community in the Huasteca Potosina (Mexico). A hundred percent of the studied population presented total AFB₁-DNA and AFB₁-lys adducts in concentrations of 1.08 (0.48–1.34) μmol of adduct/mol of DNA and 2.33 (1.08-102.6) pg/mg of albumin respectively (median (min-max)). Thus, continuous monitoring and important changes in regulations are desired and recommended. The results in this study provide enough evidence to support the toxic effects that the exposure to AFB₁ represents, as well as the population risk that it poses, and in the same sense, the current need to create an intervention program that directly influences the control of the sources of exposure in order to reduce it.
اظهر المزيد [+] اقل [-]Effect of acid-treated and hexadecyltrimethylammonium bromide–modified montmorillonites on adsorption performance of mycotoxins
2020
Mao, Jiaqi | Lv, Guanglie | Zhou, Renxian
A series of modified montmorillonites treated by acid and hexadecyltrimethylammonium bromide (HTAB) were prepared and characterized, and their adsorption performances for three mycotoxins (aflatoxin B1, zearalenone, and deoxynivalenol) were evaluated at pH 2.8 and 8.0, respectively. The results indicate that the layers of raw montmorillonite are exfoliated after acid treatment and more active sites for adsorption of weak polar mycotoxins are exposed. While the intercalation of HTAB leads to an obvious increase of the interlamellar spacing and hydrophobic character of montmorillonite. The HTAB-AMMT-3 modified by acid and HTAB exhibits excellent adsorption capacity towards aflatoxin B1 (AFB1) and zearalenone (ZEA) whether in acidic or alkaline conditions compared with raw montmorillonite (MMT). However, all modified montmorillonites have low adsorption capacity for DON due to its poor planarity preventing it from entering into interfacial layer of montmorillonite.
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