Anthelmintics, drugs against parasitic worms, are frequently used in livestock and might act as danger environmental microcontaminants. The present study was designed to monitor the possible circulation of common anthelmintic drug albendazole (ABZ) and its metabolites in the real agriculture conditions. The sheep were treated with the recommended dose of ABZ. Collected faeces were used for the fertilization of a field with fodder plants (alfalfa and clover) which served as feed for sheep from a different farm. The selective ultrasensitive mass spectrometry revealed surprisingly high concentrations of active ABZ metabolite (ABZ-sulphoxide) in all samples (dung, plants, ovine plasma, rumen content and faeces). Our results prove for the first time an undesirable permeation of ABZ metabolites from sheep excrement into plants (used as fodder) and subsequently to other sheep in real agricultural conditions. This circulation causes the permanent exposition of the ecosystems and food-chain to the drug and can promote the development of drug resistance in helminths.

This study performs an environmental risk assessment (ERA) of the anthelmintic medicine albendazole (ABZ) in the eastern African region. A systematic literature search strategy was applied to obtain quantitative information on the physicochemical characteristics, the metabolization-fate, the ecotoxicity and the environmental occurrence in different countries worldwide serving as model regions. In addition, insilico tools were employed to obtain data on physicochemical characteristics and toxic hazards of ABZ and its metabolites. Moreover, ERA models were used to predict environmental concentrations in different compartments and compare them with the measured environmental concentrations. Finally, the environmental risk of ABZ in the eastern Africa was estimated by calculating the risk quotient (RQ), and its uncertainty estimated by Monte Carlo simulation. The predicted environmental concentrations of ABZ in surface water in the model region based on consumption (1.6–267 ng/L) were within the range of values obtained from the measured environmental concentrations of the same region (0.05–101,000 ng/L). Using these models with adapted input variables for eastern Africa, the predicted surface water concentration in that region was 19,600 ± 150 ng/L (95% CI). The calculated soil concentrations of ABZ in the model regions and the eastern Africa were found to be 0.057 ± 0.0 μg/kg and 0.022 ± 0.0 μg/kg, respectively. The environmental risk expressed as risk quotient of ABZ in eastern Africa estimated for the aquatic compartment (146 ± 1) indicated a significant environmental risk calling on appropriate actions from the competent authorities to reduce this risk in this region.

Albendazole (ALB) belongs to a group of benzimidazoles - classified as antiparasitic pharmaceuticals. Its widespread application results in the presence of this pharmaceutical in natural environment (water and soil). In this paper a suitable pretreatment method was established including sampling, freeze-drying and extraction. Vicia faba was used as model organism. ALB accumulation by plant tissues was observed in hydroponic culture as well as in soil. The range of pharmaceutical concentrations was 1.7 × 10-5 mol/L (in hydroponic culture) and 1.7 × 10-5 to 1.7 × 10-4 mol/kg air dry soil (in soil). Observations were conducted for 14 days. After this time biological material was freeze-dried and after homogenization, dimethyl sulfoxide (DMSO) extraction was performed. The recovery of ALB for the roots was 93 % while for the shoots 86 %. After cleaning, the samples were subjected to further analysis by HPLC system. Phosphate buffer and acetonitrile (50:50) were used as a mobile phase. Drug retention time was 6.3 min. Results obtained in this experiment indicate higher drug accumulation in roots rather than in the hypocotyl part of the plant, cultivated both in soil and in hydroponic culture. © 2013 The Author(s).

Anthelmintics (AHs) control animal infections with gastrointestinal nematodes. They reach soil through animal faeces deposited on soils or through manuring. Although soil constitutes a major AH sink, we know little about the mechanisms controlling their soil dissipation. We employed studies with fumigated and non-fumigated soils collected from 12 sheep farms with a variable record of albendazole (ABZ), ivermectin (IVM) and eprinomectin (EPM) use. From each farm, we collected soils from inside small ruminant barn facilities (series A, high exposure) and the associated grazing pastures (series B, low exposure). We asked the following questions: (a) What is the role of soil microorganisms in AH dissipation? (b) Does repeated exposure of soils to AHs lead to their accelerated biodegradation? (c) Which soil physicochemical properties control AH dissipation? Soil fumigation significantly retarded ABZ (DT₅₀ 1.9 and 4.33 days), IVM (34.5 and 108.7 days) and EPM dissipation (30 and 121 days) suggesting a key role of soil microorganisms in AH dissipation. No significant acceleration in AH dissipation was evident in soils from units with a record of the administration of AHs or in soil series A vs series B, suggesting that the level of prior exposure was not adequate to induce their enhanced biodegradation. Significant positive and negative correlations of soil total organic carbon (TOC) and ABZ and IVM dissipation, respectively, were observed. Soil adsorption of AHs increased in the order IVM > ABZ > EPM. TOC controlled soil adsorption of IVM and EPM, but not of ABZ, in support of the contrasting effect of TOC on IVM and ABZ dissipation.

Benzimidazoles (BZ) are among the most used drugs to treat parasitic diseases in both human and veterinary medicine. In this study, solutions fortified with albendazole (ABZ), fenbendazole (FBZ), and thiabendazole (TBZ) were subjected to photoperoxidation (UV/H₂O₂). The hydroxyl radicals generated by the process removed up to 99% of ABZ, and FBZ, in the highest dosage of H₂O₂ (i.e., 1.125 mmol L⁻¹; 4.8 kJ L⁻¹). In contrast, 20% of initial TBZ concentration remained in the residual solution. In the first 5 min of reaction (i.e., up to 0.750 mmol L⁻¹ of H₂O₂), formation of the primary metabolites of ABZ—ricobendazole (RBZ), albendazole sulfone (ABZ-SO₂), and oxfendazole (OFZ)—was observed. However, these reaction products were converted after the reaction time was doubled. The residual ecotoxicity was investigated using the Raphidocelis subcapitata microalgae and the marine bacteria Vibrio fischeri. The results for both microorganisms evidence that the residual solutions are less harmful to these microorganisms. However, after 30 min of reaction, the treated solution still presents a toxic effect for V. fischeri, meaning that longer reaction times are required to achieve an innocuous effluent.

Benzimidazoles are anthelmintics frequently used in sheep farming due to the high susceptibility of these animals to parasitic diseases. Sheep excreta are often disposed onto soils as a fertilizer, and they may contain benzimidazole residues that can contaminate soil and water. This work aimed to assess the leaching behavior of benzimidazole drugs (albendazole, fenbendazole, and thiabendazole) and their metabolites in two Brazilian soils of different textural classifications (sandy and clay), as well as sheep excreta-amended soils, following the OECD 312 Guidelines. Ewes received a single oral dose of 10 mg kg⁻¹ b.w. of either albendazole or fenbendazole. The feces were collected at 24, 48, 72, 96, and 120 h post-dose, and the parent drugs and their metabolites extracted using the QuEChERS approach and quantified by UHPLC-MS/MS. For the leaching assays, a benzimidazole solution was directly applied onto the soil columns, or an amount of 5 g of the medicated sheep feces was distributed over the top of the soil columns. In soil samples, benzimidazoles were extracted by solid-liquid extraction and quantified by UHPLC-MS/MS. For the leaching studies, atrazine was used as a reference substance to determine the relative mobility factor of the analytes of interest. Benzimidazoles were considered slightly to moderately mobile in both soils tested, with a leaching distance of up to 25 cm in a 30-cm soil column. Approximately 3 to 6% of the benzimidazoles present in ewe feces were able to leach into the soil columns. This finding is of concern since benzimidazoles are persistent in soil and may pose a risk to soil biota and induce the development of resistant strains of parasites.

Copper (Cu) is one of the most common metal contaminants, and albendazole (ABZ) is a veterinary drug with a high efficacy against helminthes. It is believed that the two may co-exist in soil. In this study, the combined subacute toxicity of Cu exposure (0, 80, 120, 160 mg kg⁻¹) and ABZ exposure (0, 3, 9 mg kg⁻¹) in earthworms (Eisenia fetida) were observed using three approaches, namely chronic growth and reproduction, antioxidant enzyme activity, and earthworm Cu residue. The results have shown that the toxicity of Cu on cocoon hatching success and biomass was alleviated by presence of low concentrations of ABZ (3 mg kg⁻¹) during a 56-day exposure period. However, the sensitivity of the earthworms’ reproduction to Cu increased with the presence of high concentrations of ABZ (9 mg kg⁻¹), indicating a reduction beginning at a Cu concentration of 80 mg kg⁻¹, in the cocoon number, hatching success, and biomass. In addition, the three enzyme activities exhibited different responsive patterns, indicating inducement in the catalase and glutathione peroxidase, and inhibition in the superoxide dismutase, which were dependent on the exposure times and concentrations. In regard to the earthworm Cu residue, when increasing Cu exposure concentrations, the internal Cu concentrations tended to level off, exhibited a linear pattern at the Cu concentration range of 40 to 120 mg kg⁻¹, and showed a stable trend above 120 mg kg⁻¹. The results of the present study can potentially provide important information regarding the combined toxicity of the veterinary drugs and the heavy metals in soil.