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Using hydrogen peroxide to control cyanobacterial blooms: A mesocosm study focused on the effects of algal density in Lake Chaohu, China
2021
Chen, Chao | Wang, Yiyao | Chen, Kaining | Shi, Xiaoli | Yang, Gang
The application of hydrogen peroxide (H₂O₂) to control harmful algal blooms is affected by algal density and species. In the present study, a simulation field study was carried out to evaluate the removal of cyanobacteria with high algal density (chlorophyll a of approximately 220–250 μg/L) and low algal density (chlorophyll a of approximately 30–50 μg/L) using 10, 20 mg/L H₂O₂ and 5 mg/L H₂O₂. The dynamics of algal biomass, nutrients, microcystins, phytoplankton, and zooplankton were measured within 7 d. The results showed that 5 mg/L H₂O₂ effectively eliminated algal biomass (measured as chlorophyll a and phycocyanin) and inhibited 50% of the photosynthetic activity of the cyanobacteria at 7 d in the low algal cell density group, while the same inhibition rate was observed in the high algal cell density group when the H₂O₂ was 20 mg/L. However, using a high dosage of H₂O₂, such as 10 mg/L, to suppress cyanobacteria with high biomass could result in a dramatic increase in nutrients and microcystins in the water column. The portion of eukaryotic algae, such as Chlorophyta, Bacillariophyta and Euglenophyta, in the phytoplankton community increased with increasing H₂O₂ concentrations; moreover, the dominant species of cyanobacteria changed from the nontoxic genus Dactylococcopsis to the toxic genus Oscillatoria, which may result in acute toxicity to zooplankton. Our results demonstrated that the application of H₂O₂ to control cyanobacterial blooms at the early stage when algal cell density was low posed less potential ecological risks and may have increased the diversity of the phytoplankton community.
اظهر المزيد [+] اقل [-]Parental and trophic transfer of nanoscale plastic debris in an assembled aquatic food chain as a function of particle size
2021
Monikh, Fazel Abdolahpur | Chupani, Latifeh | Vijver, Martina G. | Peijnenburg, Willie J.G.M.
The existing limitations in analytical techniques for characterization and quantification of nanoscale plastic debris (NPD) in organisms hinder understanding of the parental and trophic transfer of NPD in organisms. Herein, we used iron oxide-doped polystyrene (PS) NPD (Fe-PS-NPD) of 270 nm and Europium (Eu)-doped PS-NPD (Eu-PS-NPD) of 640 nm to circumvent these limitations and to evaluate the influence of particle size on the trophic transfer of NPD along an algae-daphnids food chain and on the reproduction of daphnids fed with NPD-exposed algae. We used Fe and Eu as proxies for the Fe-PS-NPD and Eu-Ps-NPD, respectively. The algae cells (Pseudokirchinella subcapitata) were exposed to 4.8 × 10¹⁰ particles/L of Fe-PS-NPD or Eu-PS-NPD for 72 h. A high percentage (>60%) of the NPD was associated with algal cells. Only a small fraction (<11%) of the NPD, however, was transferred to daphnids fed for 21 days on the NPD-exposed algae. The uptake and trophic transfer of the 270 nm Fe-PS-NPD were higher than those for the 640 nm Eu-PS-NPD, indicating that smaller NPD are more likely to transfer along food chains. After exposure to Fe-PS-NPD, the time to first brood was prolonged and the number of neonates per adult significantly decreased compared to the control without any exposure and compared to daphnids exposed to the Eu-Ps-NPD. The offspring of daphnids exposed to Eu-PS-NPD through algae, showed a traceable concentration of Eu, suggesting that NPD are transferred from parents to offspring. We conclude that NPD can be transferred in food chains and caused reproductive toxicity as a function of NPD size. Studies with prolonged exposure and weathered NPD are endeavored to increase environmental realism of the impacts determined.
اظهر المزيد [+] اقل [-]Study of a hydrodynamic threshold system for controlling dinoflagellate blooms in reservoirs
2021
Song, Yang | Shen, Lian | Zhang, Linglei | Li, Jia | Chen, Min
Hydrodynamic conditions often affect the eutrophication process and play a key role in algal growth in reservoirs. A promising approach for controlling algal blooms in reservoirs is to create adverse hydrodynamic conditions by implementing reservoir operation strategies. However, research on this method is still nascent and does not support practical applications due to the lack of quantitative hydrodynamic thresholds. In this paper, field observations of algal growth from April 2015 to August 2016 were conducted, and a three-dimensional (3D) model that couples hydrodynamics and water temperatures for the Zipingpu Reservoir was established. Low flow velocities (V) and low Reynolds numbers (Re) in the Longchi tributary are favorable for dinoflagellate growth and accumulation, which can explain why dinoflagellate blooms are more likely to occur in the tributary. A temperature of 18–22 °C is considered a precondition for Peridiniopsis penardii blooms, suggesting that freshwater dinoflagellate species may prefer lower temperatures than marine dinoflagellate species. Shallow mixing layer depth (Zₘᵢₓ) is conducive to Peridiniopsis penardii gathering in the upper water layers and promotes growth. The shallow euphotic layer depth (Zₑᵤ) was speculated to promote the dominance of this species by stimulating its heterotrophy and inhibiting other algal autotrophy. Furthermore, a boundary line analysis was introduced to characterize the relationships between algal biomass and hydrodynamic indicators. Thus, the thresholds for V, Re, and Zₘᵢₓ/Zₑᵤ were determined to be 0.034 m s⁻¹, 6.7 × 10⁴, and 1.7, respectively. Either accelerating horizontal flow to exceed the thresholds of V and Re or facilitating vertical mixing to exceed the threshold of Zₘᵢₓ/Zₑᵤ can prevent dinoflagellate blooms. Therefore, the summarized hydrodynamic threshold system is suggested to be an effective standard for controlling dinoflagellate blooms in the reservoir. Moreover, this study can provide a useful reference for understanding the mechanism of freshwater dinoflagellate blooms.
اظهر المزيد [+] اقل [-]Sequestration of microfibers and other microplastics by green algae, Cladophora, in the US Great Lakes
2021
Peller, Julie | Nevers, Meredith B. | Byappanahalli, Muruleedhara | Nelson, Cassie | Ganesh Babu, Bharath | Evans, Mary Anne | Kostelnik, Eddie | Keller, Morgan | Johnston, Jenna | Shidler, Sarah
Daunting amounts of microplastics are present in surface waters worldwide. A main category of microplastics is synthetic microfibers, which originate from textiles. These microplastics are generated and released in laundering and are discharged by wastewater treatment plants or enter surface waters from other sources. The polymers that constitute many common synthetic microfibers are mostly denser than water, and eventually settle out in aquatic environments. The interaction of these microfibers with submerged aquatic vegetation has not been thoroughly investigated but is potentially an important aquatic sink in surface waters. In the Laurentian Great Lakes, prolific growth of macrophytic Cladophora creates submerged biomass with a large amount of surface area and the potential to collect and concentrate microplastics. To determine the number of synthetic microfibers in Great Lakes Cladophora, samples were collected from Lakes Erie and Michigan at multiple depths in the spring and summer of 2018. After rinsing and processing the algae, associated synthetic microfibers were quantified. The average loads of synthetic microfibers determined from the Lake Erie and Lake Michigan samples were 32,000 per kg (dry weight (dw)) and 34,000 per kg (dw), respectively, 2–4 orders of magnitude greater than loads previously reported in water and sediment. To further explore this sequestration of microplastics, fresh and aged Cladophora were mixed with aqueous mixtures of microfibers or microplastic in the laboratory to simulate pollution events. Microscopic analyses indicated that fresh Cladophora algae readily interacted with microplastics via adsorptive forces and physical entanglement. These interactions mostly cease upon algal senescence, with an expected release of microplastics in benthic sediments. Collectively, these findings suggest that synthetic microfibers are widespread in Cladophora algae and the affinity between microplastics and Cladophora may offer insights for removing microplastic pollution.Macroalgae in the Laurentian Great Lakes contain high loads of synthetic microfibers, both entangled and adsorbed, which likely account for an important fraction of microplastics in these surface waters.
اظهر المزيد [+] اقل [-]The combined effects of macrophytes and three road salts on aquatic communities in outdoor mesocosms
2021
Coldsnow, Kayla D. | Relyea, Rick A.
Because of environmental and societal concerns, new strategies are being developed to mitigate the effects of road salt. These include new deicers that are alternatives to or mixtures with the most common road salt, sodium chloride (NaCl), improved techniques and equipment, and biotic mitigation methods. Using outdoor mesocosms, we investigated the impacts of NaCl and two common alternatives, magnesium chloride (MgCl₂) and calcium chloride (CaCl₂) on freshwater communities. We also investigated the mitigation ability of a common macrophyte, Elodea. We hypothesized that road salt exposure reduces filamentous algae, zooplankton, and macrocrustaceans, but results in increases in phytoplankton and gastropods. We also hypothesized that MgCl₂ is the most toxic salt to communities, followed by CaCl₂, and then NaCl. Lastly, we hypothesized that macrophytes mitigate some of the effects of road salt, specifically the effects on primary producers. We found that all three salts reduced filamentous algal biomass and amphipod abundance, but only MgCl₂ reduced Elodea biomass. MgCl₂ had the largest and longest lasting effects on zooplankton, specifically cladocerans and copepods, which resulted in a significant increase in phytoplankton and rotifers. CaCl₂ increased ostracods and decreased snail abundance, but NaCl increased snail abundance. Lastly, while we did not find many interactions between road salt and macrophyte treatments, macrophytes did counteract many of the salt effects on producers, leading to decreased phytoplankton, increased filamentous algae, and altered abiotic responses. Thus, at similar chloride concentrations, NaCl alternatives, specifically MgCl₂, are not safer for aquatic ecosystems and more research is needed to find safer road management strategies to protect freshwater ecosystems.
اظهر المزيد [+] اقل [-]A simple and rapid algal assay kit to assess toxicity of heavy metal-contaminated water
2021
Eom, Heonseop | Park, Minseung | Jang, Am | Kim, Seunggyu | Oh, Sang-Eun
This study presents a novel algal-based toxicity test suitable for simple and rapid assessment of heavy metal (Hg2+, Cr6+, Cd2+, Pb2+, or As3+)-contaminated water. A closed-system kit-type algal assay was developed using Chlorella vulgaris. Toxicity was assessed by oxygen evolution in the gaseous phase of the assay kits, which was measured via a needle-type oxygen sensor. Initial cell density, light intensity, and exposure time that enabled favorable test performance for the algal assay kits were 103 cells/mL, 250 μmol m-2s-1, and 18 h, respectively. Results from the heavy metal toxicity tests demonstrate that Hg2+, Cr6+, Cd2+, and Pb2+ are more toxic in inhibiting algal photosynthetic activity than As3+. The 18 h half-maximum effective concentrations (EC50) for Hg2+, Cr6+, Cd2+, Pb2+, and As3+ were determined to be 31.3 ± 0.5, 179.6 ± 7.5, 301.3 ± 6.1, 476.1 ± 10.5, and 2184.1 ± 31.1 μg/L, respectively. A strong correlation between oxygen concentrations in the headspace of the assay kits and chlorophyll a production indicates that oxygen evolution in the gaseous phase is able to represent algal photosynthetic activity and serve as the end-point in algal toxicity tests. High test sensitivity and reproducibility as well as an easy test protocol and rapid processing time make the algal assay kit a suitable tool for simple and rapid toxicity testing of heavy metal-contaminated water.
اظهر المزيد [+] اقل [-]Toxicity assessment and underlying mechanisms of multiple metal organic frameworks using the green algae Chlamydomonas reinhardtii model
2021
Lee, Patsy | Shang, Shanshan | Shang, Jin | Wang, Wen-Xiong
Metal-organic frameworks (MOFs) are an emerging class of materials which have garnered increasing attention for their utility as adsorbents and photocatalysts in water treatment. Nevertheless, the environmental risks of MOFs, especially their underlying impacts on aquatic organisms, are not fully explored. Herein, the toxicity of multiple representative MOFs was systematically assessed using a freshwater green alga (Chlamydomonas reinhardtii) model. Six typical MOFs with different metal nodes or organic linkers, including four transition metal incorporated aluminum-based porphyrin MOFs [pristine Al-PMOF, Al-PMOF (Cu), Al-PMOF (Ni), and Al-PMOF (Co)], one amine-functionalized MOF NH₂-MIL-125 (Ti), and one bimetallic Hofmann MOF (NiCo-PYZ), were successfully synthesized and characterized. All the tested MOFs significantly reduced the chlorophyll content and inhibited the algal growth, with the most toxic materials being NiCo-PYZ and Al-PMOF (Cu). Distinct toxic mechanisms were observed for the tested MOFs. Metal ion release was the primary cause for algal toxicity induced by NiCo-PYZ. The algal toxicity induced by porphyrin MOFs could be explained by the combined effects of metal ion release and nutrient adsorption, agglomeration and physical interactions, and reactive oxygen species generation. NH₂-MIL-125 (Ti) showed higher stability and more biocompatibility than the other tested MOFs. MOFs concentrations with no harmful effects to algae can be taken as the threshold values for safe use and discharge of MOFs. The ecotoxicological risks of MOFs should be considered as the applied concentrations of MOFs at mg/mL levels in environmental remediation were much higher than the no harmful effect thresholds.
اظهر المزيد [+] اقل [-]Diversity and predicted inter- and intra-domain interactions in the Mediterranean Plastisphere
2021
Amaral-Zettler, Linda A. | Ballerini, Tosca | Zettler, Erik R. | Asbun, Alejandro Abdala | Adame, Alvaro | Casotti, Raffaella | Dumontet, Bruno | Donnarumma, Vincenzo | Engelmann, Julia C. | Frère, Laura | Mansui, Jeremy | Philippon, Marion | Pietrelli, Loris | Sighicelli, Maria
This study investigated the biogeography, the presence and diversity of potentially harmful taxa harbored, and potential interactions between and within bacterial and eukaryotic domains of life on plastic debris in the Mediterranean. Using a combination of high-throughput DNA sequencing (HTS), Causal Network Analysis, and Scanning Electron Microscopy (SEM), we show regional differences and gradients in the Mediterranean microbial communities associated with marine litter, positive causal effects between microbes including between and within domains of life, and how these might impact the marine ecosystems surrounding them. Adjacent seas within the Mediterranean region showed a gradient in the microbial communities on plastic with non-overlapping endpoints (Adriatic and Ligurian Seas). The largest predicted inter-domain effects included positive effects of a novel red-algal Plastisphere member on its potential microbiome community. Freshwater and marine samples housed a diversity of fungi including some related to disease-causing microbes. Algal species related to those responsible for Harmful Blooms (HABs) were also observed on plastic pieces including members of genera not previously reported on Plastic Marine Debris (PMD).
اظهر المزيد [+] اقل [-]Biomass utilization and production of biofuels from carbon neutral materials
2021
Srivastava, Rajesh K. | Shetti, Nagaraj P. | Reddy, Kakarla Raghava | Kwon, Eilhann E. | Nadagouda, Mallikarjuna N. | Aminabhavi, Tejraj M.
The availability of organic matters in vast quantities from the agricultural/industrial practices has long been a significant environmental challenge. These wastes have created global issues in increasing the levels of BOD or COD in water as well as in soil or air segments. Such wastes can be converted into bioenergy using a specific conversion platform in conjunction with the appropriate utilization of the methods such as anaerobic digestion, secondary waste treatment, or efficient hydrolytic breakdown as these can promote bioenergy production to mitigate the environmental issues. By the proper utilization of waste organics and by adopting innovative approaches, one can develop bioenergy processes to meet the energy needs of the society. Waste organic matters from plant origins or other agro-sources, biopolymers, or complex organic matters (cellulose, hemicelluloses, non-consumable starches or proteins) can be used as cheap raw carbon resources to produce biofuels or biogases to fulfill the ever increasing energy demands. Attempts have been made for bioenergy production by biosynthesizing, methanol, n-butanol, ethanol, algal biodiesel, and biohydrogen using different types of organic matters via biotechnological/chemical routes to meet the world’s energy need by producing least amount of toxic gases (reduction up to 20–70% in concentration) in order to promote sustainable green environmental growth. This review emphasizes on the nature of available wastes, different strategies for its breakdown or hydrolysis, efficient microbial systems. Some representative examples of biomasses source that are used for bioenergy production by providing critical information are discussed. Furthermore, bioenergy production from the plant-based organic matters and environmental issues are also discussed. Advanced biofuels from the organic matters are discussed with efficient microbial and chemical processes for the promotion of biofuel production from the utilization of plant biomasses.
اظهر المزيد [+] اقل [-]Phosphorus deficiency stimulates dominance of Cylindrospermopsis through facilitating cylindrospermopsin-induced alkaline phosphatase secretion: Integrating field and laboratory-based evidences
2021
Lu, Zhe | Lei, Lamei | Lu, Yan | Peng, Liang | Han, Boping
Potentially toxic Cylindrospermopsis raciborskii blooms are of emerging concerns, as its scale is spreading from tropical regions to high latitudes, increasing the risk of aquatic biota being exposed to cylindrospermopsin (CYN). So far, CYN-producing C. raciborskii strains have only been reported in tropical waters which are commonly phosphorus (P)-deficient, where they can dominate phytoplankton communities. However, the influence of CYN on phytoplankton communities under different P status remains unclear. In this study, we first analyzed the summer observations of 120 tropical reservoirs in Guangdong Province. The proportion of potential CYN-producers was significantly higher in P-deficient and CYN-present reservoirs than that in P-sufficient or CYN-absent ones. This suggested that in P-deficient condition, the potential CYN producers might gain more advantages by the help of CYN. Then, in laboratory experiments we found that upon P deprivation, CYN did not inhibit the cell growth of other algal cells, but significantly stimulates them to secret more alkaline phosphatase (ALP) than in P-sufficient condition. Through transcriptomics, we further revealed that under such P-deficient condition, CYN remarkably induced intracellular nitrogen allocation and protein export system by activating the PIK3/Akt-cGMP/PKG signaling pathways in Scenedesmus bijugatus, thus enhancing its ALP secretion. Our study implies that CYN-induced ALP secretion is facilitated upon P deficiency, thus supporting the dominance of its producers C. raciborskii.
اظهر المزيد [+] اقل [-]