The individual effects of biological constituents of particulate matter (PM) such as fungal spores, on lung function in children are not well known. This study investigated the seasonal short-term effect of daily variation in Alternaria and Cladosporium fungal spores on lung function in schoolchildren.This panel study evaluated 313 schoolchildren in informal settlements of the Western Cape of South Africa, exposed to spores of two commonly encountered fungi, Alternaria and Cladosporium species. The children provided forced-expiratory volume in 1-s (FEV₁) and peak-expiratory flow (PEF) measurements thrice daily for two consecutive school-weeks in summer and winter. Daily PM₁₀ levels, from a stationary ambient air quality monitor and fungal spore levels using spore traps were measured in each study area throughout the year. The effects of Alternaria and Cladosporium spores, on lung function were analysed for lag periods up to five-days, adjusting-for PM₁₀, other pollen exposures, study area, and other host and meteorological factors. Same-day exposure-response curves were computed for both fungal species.There was more variability in Alternaria spores level with noticeable peaks in summer. There were consistent lag-effects for Alternaria on PEF compared to Cladosporium, with the largest PEF deficit observed in winter (mean deficit: 13.78 L/min, 95%CI: 24.34 to −3.23 L/min) per 10spores/m³ increase in Alternaria spores on lag day-2. Although there were no observable lag-effects for Alternaria and Cladosporium on FEV₁, same-day effects of Cladosporium spores on FEV₁ was present across both seasons. Threshold effects of Alternaria on both PEF and FEV₁ deficits were apparent at levels of 100 spores/m³, but could not be explored for Cladosporium beyond the levels observed during the study.The study provides evidence for the independent effects of daily exposure to ambient fungal spores of Alternaria and Cladosporium on lung function deficits, more especially in winter for PEF.

Open landfilling is a common practice of waste dumping in developing countries, generating a range of environmental and public health hazards. In this study, we determined the distribution, composition and exposure risk of the size-segregated fungal and bacterial bioaerosols in an open landfill site in Dehradun, India. Bioaerosol and particulate matter (PM) measurements were carried out using a six-stage viable Andersen Cascade Impactor and a Wide Range Aerosol Spectrometer, respectively. Pearson correlation analysis was used to determine the relation of bioaerosol concentration with meteorological parameters and PM. The mean concentration of fungal and bacterial aerosols was observed to be 4582.75 ± 1358.25 CFU/m3 and 3609.53 ± 1000.28 CFU/m3, respectively. The bioaerosol composition showed the predominance of potential pathogens including Aspergillus (25.42%), Penicillium (20.34%), Cladosporium (15.25%), Alternaria (13.56%); and gram-negative Bacillus (21.15%), Streptobacillus (17.31%), Coccus (13.46%). Also, an inhalation risk assessment was conducted for the age-specific predictions of bioaerosol and PM deposition in human airways using a multiple path particle dosimetry model. For bioaerosols, maximum submicron depositions in the pulmonary region were observed in adults, while for PM, in children and infants. Finally, to identify the bioaerosol exposure caused respiratory disorders, a questionnaire-based health survey was conducted among the exposed population around the landfill site. The chi-square test showed significantly higher respiratory complaints in females for cold, cough, chest pain and chest tightness than in males. This work highlights the role of bioaerosols and PM in human health disorders in occupational environments associated with waste management.

The possibility of superficial foot mycoses to spread through contaminated mosque carpets between worshipers imposes a great health burden and is never addressed in Riyadh, SA. We aimed to assess the prevalence of fungal infections, especially human dermatophytosis acquired from mosque carpets, in five different regions of Riyadh, Saudi Arabia and establish a means of contamination control. A cross-sectional study of 100 mosques in Riyadh, Saudi Arabia, was conducted, using a multistage random cluster sampling technique. The study included mosques that had an ablution area and regularly hosted five prayers a day. Sterile swabs were used for sampling, and the samples were transported to the laboratory for culture and analysis using a micro-scan system. A total of 199 (65%) samples contained several fungal species. Rhodotorula (74%), Cladosporium (47%), Bipolaris (46.6%), other yeasts (43.7%), Alternaria (40%), dematiaceous molds (34%), Curvularia (32.4%), and Candida (31%) were the most frequently isolated species. Species belonging to several other genera were also detected. This study revealed a relatively high prevalence of fungal organisms in mosque carpets in Riyadh, suggesting the need for implementing new strategies and laws to increase the level of hygiene awareness among worshipers and mosque caretakers to limit the spread of foot fungal infections.

Atopic asthma is characterized by the presence of sensitization to common aeroallergens, which tends to have a worse prognosis than non-atopic asthma. The objectives were to determine the prevalence of aeroallergens sensitization in the Lebanese pediatric asthmatic population and determine the relationship between allergens sensitization (indoor and outdoor) and age, area of residence and altitude. A sample, consisting of 919 asthmatic children (aged 1 to 18 years, from 2010 until 2017), underwent skin prick testing (SPT) with 21 common allergens: 5 grasses (cocksfoot, sweet vernal-grass, rye-grass, meadow grass, timothy), Parietaria, olive, Dermatophagoides pteronyssinus and Dermatophagoides farina (DP-DF), dog and cat dander, Alternaria longipens, Aspergillus fumigatus and nidulans, Cupressaceae, pine, German cockroach, and 4 cereals (oat, wheat, barley, maize). Seven hundred fifty-two patients had positive SPT. The distribution of sensitization was as follows: DP-DF 59%; 5 grasses 34%; 4 cereals 33.9%; cat 29.9%; Alternaria 27.9%; Parietaria 23%; dog 21.9%; olive 20.5%; Aspergillus mix 18.6%; Cupressaceae 18.2%; pine 17%; cockroach 15.3%. House dust mites sensitization was frequent at lower altitude (< 900 m) (56.3%) and in the whole country (a median prevalence of 53.05%) except for the Beqaa region (negative HDM in 82.4%). Non-atopic asthma was more frequent in early childhood (40.5% at 1–4 years vs 11.2% at 11–18 years). The sensitization rate increased with age, starting at 5 years. Higher age (aOR = 1.24) and altitude less than 900 m compared with ≥ 900 m (aOR = 2.03) were significantly associated with the presence of aeroallergens in children. House dust mites and grasses are the most common allergens in Lebanese asthmatic children. Non-atopic asthma is more frequent at early age. Lebanese children with asthma showed a polysensitized pattern starting at 5 years.