International audience | Macrophytes are known to bioaccumulate metals, but a thorough understanding of tolerance strategies and molecular impact of metals in aquatic plants is still lacking. The present study aimed to compare Hg and Cd effects in a representative macrophyte, Elodea nuttallii using physiological endpoints and metabolite profiles in shoots and cytosol. Exposure 24 h to methyl-Hg (30 ng L), inorganic Hg (70 ng L) and Cd (280 μg L) did not affect photosynthesis, or antioxidant enzymes despite the significant accumulation of metals, confirming a sublethal stress level. In shoots, Cd resulted in a higher level of regulation of metabolites than MeHg, while MeHg resulted in the largest number of regulated metabolites and IHg treatment regulated no metabolites significantly. In cytosol, Cd regulated more metabolites than IHg and only arginine, histidine and mannose were reduced by MeHg exposure. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of data suggested that exposure to MeHg resulted in biochemical changes including aminoacyl-tRNA biosynthesis, glycine, serine and threonine metabolism, nitrogen metabolism, arginine and proline metabolism, cyanoamino acid metabolism, while the treatment of Cd stress caused significant variations in aminoacyl-tRNA biosynthesis and branched-chain amino acids pathways. Data supports an impact of MeHg on N homeostasis, while Cd resulted in an osmotic stress-like pattern and IHg had a low impact. Marked differences in the responses to MeHg and IHg exposure were evidenced, supporting different molecular toxicity pathways and main impact of MeHg on non-soluble compartment, while main impact of IHg was on soluble compartment. Metabolomics was used for the first time in this species and proved to be very useful to confirm and complement recent knowledge gained by transcriptomics and proteomics, highlighting the high interest of multi-omics approaches to identify early impact of environmental pollution.

Surface modified lipopeptide biosurfactant (BS) with enhancement of amino acids was produced using Bacillus Malacitensis. The aromatic hydrocarbons from contaminated soil were removed by BS soil washing process and bioremediation using activated functionalized carbon-BS matrix (AFC-BS). The Central Composite Design (CCD) showed the optimum time100 h; pH 7; temperature 30°C on maximum yield of BS. The amino acid profiling of BS reveals the enhancement of amino acids especially polar amino acids and its importance in the formation of micellar structure for the tight packing of aromatic hydrocarbons from industrial contaminated soil. AFC-BS matrix was implanted directly into the contaminated soil for 28 days and found 61.80 % of Total Petroleum Hydrocarbon (TPH) removal efficiency which is high compared to the AFC treated soil. The compounds were extracted from contaminated soil and AFC-BS matrix, found similar peaks in high performance liquid chromatography, which reveals the ability of BS to remove aromatic contaminants. The soil toxicity was also analyzed by seed germination and found improvement in the growth of seeds. The germination of seeds increased from 60 % to 100 % and the phytotoxicity of root and shoot was reduced from 89.50 %, 88.45 % to12.55 %, 11.87 % respectively.

Selenium (Se) radioactive wastes can be disposed through stabilization/solidification (S/S) based on the cementitious matrix on hydration products, where hydrocalumite (Ca₂Al-LDH) is expected to play an important role in the retention of SeO₄²⁻. Natural organic matters (NOMs) are known to be a risk to affect the transportation and mobility of undesirable chemical species in the pedosphere which receives the low level radioactive wastes (LLW). In the present work, five amino acids were selected as the simplified models of NOMs in the pedosphere to explore their effects on the stability of Ca₂Al-LDH after immobilized SeO₄²⁻ under alkaline conditions. As the loading amount of amino acids on Ca₂Al-LDH increasing, release of SeO₄²⁻ was enhanced in HGly, H₂Asp, and H₂Cys series, while no enhancement was observed in HPhe and HTrp series. Density functional theory (DFT) calculation predicted ion-exchange of amino acids and CO₃²⁻ with SeO₄²⁻ in a unit cell of LDH model. The intercalation of Asp²⁻ and CO₃²⁻ caused 003 peaks in XRD sharper and d₀₀₃ decreased from 8.15 Å to 7.70 Å which is assigned to Ca₂Al-LDH(Asp, CO₃). In H₂Cys series, the 003 peaks were kept broad and SeO₄²⁻ was still relatively maintained in LDH which was caused by the lower amounts of intercalated CO₃²⁻ in the presence of H₂Cys. Amino acids in the interlayer of Ca₂Al-LDH have several possible configurations, where the most stable one is prone to be in a horizontal direction through hydrogen bonds and Ca–O chemical bonds. This provides an insight on the stability of selenate immobilized in hydrocalumite, which can be produced in cement disposing in the pedosphere for a long term of burying. Not only carbonate but also small molecular organic matters like amino acids possibly give environmental impact on the mobility of low level anionic radionuclides in LDH.

The residue of simazine herbicide in the environment is known as one of pollutant stress for lizards by crippling its fitness on direct toxic effects and indirect food shortage via the food chain effects. Both stressors were considered in our experiment in the simazine exposure and food availability to lizards (Eremias argus). The results revealed that starvation significantly reduced the lizard’s energy reserve and native immune function, while the accumulation of simazine in the liver was significantly increased. Simazine caused oxidative stress in the liver of lizards, but oxidative damage only occurred in the starved lizards. Simazine also changed the energy reserves, native immune function and detoxification of well-fed lizards, while the starved lizards showed different sensitivity to simazine. Simazine or starvation treatment independently activated the lizard HPA axis, but co-treatment caused the HPA axis inhibition. Besides, according to the variations on amino acid neurotransmitters, corticosterone hormone and thermoregulatory behavior, we inferred that lizards in threatens take the appropriate strategy on energy investment and allocation through neural, endocrine and behavioral pathways to maximize benefits in dilemma. Energy allocation was necessary, while suppression on any physiological process comes at a cost that is detrimental to long-term individual fitness.

Macrophytes are known to bioaccumulate metals, but a thorough understanding of tolerance strategies and molecular impact of metals in aquatic plants is still lacking. The present study aimed to compare Hg and Cd effects in a representative macrophyte, Elodea nuttallii using physiological endpoints and metabolite profiles in shoots and cytosol.Exposure 24 h to methyl-Hg (30 ng L⁻¹), inorganic Hg (70 ng L⁻¹) and Cd (280 μg L⁻¹) did not affect photosynthesis, or antioxidant enzymes despite the significant accumulation of metals, confirming a sublethal stress level. In shoots, Cd resulted in a higher level of regulation of metabolites than MeHg, while MeHg resulted in the largest number of regulated metabolites and IHg treatment regulated no metabolites significantly. In cytosol, Cd regulated more metabolites than IHg and only arginine, histidine and mannose were reduced by MeHg exposure. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of data suggested that exposure to MeHg resulted in biochemical changes including aminoacyl-tRNA biosynthesis, glycine, serine and threonine metabolism, nitrogen metabolism, arginine and proline metabolism, cyanoamino acid metabolism, while the treatment of Cd stress caused significant variations in aminoacyl-tRNA biosynthesis and branched-chain amino acids pathways. Data supports an impact of MeHg on N homeostasis, while Cd resulted in an osmotic stress-like pattern and IHg had a low impact. Marked differences in the responses to MeHg and IHg exposure were evidenced, supporting different molecular toxicity pathways and main impact of MeHg on non-soluble compartment, while main impact of IHg was on soluble compartment. Metabolomics was used for the first time in this species and proved to be very useful to confirm and complement recent knowledge gained by transcriptomics and proteomics, highlighting the high interest of multi-omics approaches to identify early impact of environmental pollution.

A field study was conducted to understand the physiological responses, yield and grain quality of an old (HUW234) and a modern (HD3118) wheat cultivar exposed to elevated ozone (O₃). The cultivars were grown under ambient O₃ (NF) and ambient +20 ppb O₃ (NF+) conditions using open-top chambers (OTCs). The comparative study of an old and a modern cultivar showed variable physiological responses under elevated O₃ exposure. Elevated O₃ in old cultivar caused high reductions in Rubisco activity (Vcₘₐₓ) and electron transport rate (J) compared to modern cultivar with simultaneous reductions in the rate of photosynthesis and chlorophyll fluorescence. In modern cultivar, high stomatal density and conductance caused higher O₃ uptake thereby triggering more damage to the adjacent stomatal cells and photosynthetic pigments coupled with reductions in photosynthetic rate and photosynthetic nitrogen use efficiency (PNUE). Modern cultivar also showed relatively high reduction in grain yield compared to old one under NF + treatment. Furthermore, grain quality traits (such as starch, protein and amino acids) of modern cultivar were better than old cultivar under ambient O₃, but showed more deterioration under NF + treatment. Results thus indicated that modern cultivar is relatively more susceptible to O₃ and showed more negative impacts on plant performance, yield and quality of grains compared to old cultivar.

The release of hexavalent chromium [Cr(VI)] into water bodies poses a major threat to the environment and human health. However, studies of the biological response to Cr(VI) are limited. In this study, a toxic bacterial mechanism of Cr(VI) was investigated using Pannonibacter phragmitetus BB (hereafter BB), which was isolated from chromate slag. The maximum Cr(VI) concentrations with respect to the resistance and reduction by BB are 4000 mg L−1 and 2500 mg L−1, respectively. In the BB genome, more genes responsible for Cr(VI) resistance and reduction are observed compared with other P. phragmitetus strains. A total of 361 proteins were upregulated to respond to Cr(VI) exposure, including enzymes for Cr(VI) uptake, intracellular reduction, ROS detoxification, DNA repair, and Cr(VI) efflux and proteins associated with novel mechanisms involving extracellular reduction mediated by electron transfer, quorum sensing, and chemotaxis. Based on metabolomic analysis, 174 metabolites were identified. Most of the upregulated metabolites are involved in amino acid, glucose, lipid, and energy metabolisms. The results show that Cr(VI) induces metabolite production, while metabolites promote Cr(VI) reduction. Overall, multi-enzyme expression and metabolite production by BB contribute to its high ability to resist/reduce Cr(VI). This study provides details supporting the theory of Cr(VI) reduction and a theoretical basis for the efficient bioremoval of Cr(VI) from the environment.

Microplastics are widespread emerging contaminants that have been found globally in the marine and freshwater ecosystem, but there is limited knowledge regarding its impact on coral reef ecosystem and underpinning mechanism. In the present study, using Pocillopora damicornis as a model, we investigated cytological, physiological, and molecular responses of a scleractinian coral to acute microplastic exposure. No significant changes were observed in the density of symbiotic zooxanthellae during the entire period of microplastic exposure, while its chlorophyll content increased significantly at 12 h of microplastic exposure. We observed significant increases in the activities of antioxidant enzymes such as superoxide dismutase and catalase, significant decrease in the detoxifying enzyme glutathione S-transferase and the immune enzyme alkaline phosphatase, but no change in the other immune enzyme phenoloxidase during the whole experiment period. Transcriptomic analysis revealed 134 significantly up-regulated coral genes at 12 h after the exposure, enriched in 11 GO terms mostly related to stress response, zymogen granule, and JNK signal pathway. Meanwhile, 215 coral genes were significantly down-regulated at 12 h after exposure, enriched in 25 GO terms involved in sterol transport and EGF-ERK1/2 signal pathway. In contrast, only 12 zooxanthella genes exhibited significant up-regulation and 95 genes down-regulation at 12 h after the microplastic exposure; genes regulating synthesis and export of glucose and amino acids were not impacted. These results suggest that acute exposure of microplastics can activate the stress response of the scleractinian coral P. damicornis, and repress its detoxification and immune system through the JNK and ERK signal pathways. These demonstrate that microplastic exposure can compromise the anti-stress capacity and immune system of the scleractinian coral P. damicornis, despite the minimal impact on the abundance and major photosynthate translocation transporters of the symbiont in the short term.

Recently, concerns have been raised that residues of glyphosate-based herbicides may interfere with the homeostasis of the intestinal bacterial community and thereby affect the health of humans or animals. The biochemical pathway for aromatic amino acid synthesis (Shikimate pathway), which is specifically inhibited by glyphosate, is shared by plants and numerous bacterial species. Several in vitro studies have shown that various groups of intestinal bacteria may be differently affected by glyphosate. Here, we present results from an animal exposure trial combining deep 16S rRNA gene sequencing of the bacterial community with liquid chromatography mass spectrometry (LC-MS) based metabolic profiling of aromatic amino acids and their downstream metabolites. We found that glyphosate as well as the commercial formulation Glyfonova®450 PLUS administered at up to fifty times the established European Acceptable Daily Intake (ADI = 0.5 mg/kg body weight) had very limited effects on bacterial community composition in Sprague Dawley rats during a two-week exposure trial. The effect of glyphosate on prototrophic bacterial growth was highly dependent on the availability of aromatic amino acids, suggesting that the observed limited effect on bacterial composition was due to the presence of sufficient amounts of aromatic amino acids in the intestinal environment. A strong correlation was observed between intestinal concentrations of glyphosate and intestinal pH, which may partly be explained by an observed reduction in acetic acid produced by the gut bacteria. We conclude that sufficient intestinal levels of aromatic amino acids provided by the diet alleviates the need for bacterial synthesis of aromatic amino acids and thus prevents an antimicrobial effect of glyphosate in vivo. It is however possible that the situation is different in cases of human malnutrition or in production animals.

Ibuprofen (IBU), as a commonly used non-steroidal anti-inflammatory drug (NSAID) and pharmaceutical and personal care product (PPCP), is frequently prescribed by doctors to relieve pain. It is widely released into environmental water and soil in the form of chiral enantiomers by the urination and defecation of humans or animals and by sewage discharge from wastewater treatment plants. This study focused on the alteration of metabolism in the adult zebrafish (Danio rerio) brain after exposure to R-(-)-/S-(+)-/rac-IBU at 5 μg L−1 for 28 days. A total of 45 potential biomarkers and related pathways, including amino acids and their derivatives, purine and its derivatives, nucleotides and other metabolites, were observed with untargeted metabolomics. To validate the metabolic disorders induced by IBU, 22 amino acids and 3 antioxidant enzymes were selected to be quantitated and determined using targeted metabolomics and enzyme assay. Stereoselective changes were observed in the 45 identified biomarkers from the untargeted metabolomics analysis. The 22 amino acids quantitated in targeted metabolomics and 3 antioxidant enzymes determined in enzyme assay also showed stereoselective changes after R-(-)-/S-(+)-/rac-IBU exposure. Results showed that even at a low concentration of R-(-)-/S-(+)-/rac-IBU, disorders in metabolism and antioxidant defense systems were still induced with stereoselectivity. Our study may enable a better understanding of the risks of chiral PPCPs in aquatic organisms in the environment.