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Combined biochar and double inhibitor application offsets NH3 and N2O emissions and mitigates N leaching in paddy fields
2022
He, Tiehu | Yuan, Junji | Xiang, Jian | Lin, Yongxin | Luo, J. (Jiafa) | Lindsey, S. B. (Stuart B.) | Liao, Xia | Liu, Deyan | Ding, Weixin
The effects of combined biochar and double inhibitor application on gaseous nitrogen (N; nitrous oxide [N₂O] and ammonia [NH₃]) emissions and N leaching in paddy soils remain unclear. We investigated the effects of biochar application at different rates and double inhibitor application (hydroquinone [HQ] and dicyandiamide [DCD]) on NH₃ and N₂O emissions, N leaching, as well as rice yield in a paddy field, with eight treatments, including conventional urea N application at 280 kg N ha⁻¹ (CN); reduced N application at 240 kg N ha⁻¹ (RN); RN + 7.5 t ha⁻¹ biochar (RNB1); RN + 15 t ha⁻¹ biochar (RNB2); RN + HQ + DCD (RNI); RNB1 + HQ + DCD (RNIB1); RNB2 + HQ + DCD (RNIB2); and a control without N fertilizer. When compared with N leaching under RN, biochar application reduced total N leaching by 26.9–34.8% but stimulated NH₃ emissions by 13.2–27.1%, mainly because of enhanced floodwater and soil NH₄⁺-N concentrations and pH, and increased N₂O emission by 7.7–21.2%, potentially due to increased soil NO₃⁻-N concentrations. Urease and nitrification inhibitor addition decreased NH₃ and N₂O emissions, and total N leaching by 20.1%, 21.5%, and 22.1%, respectively. Compared with RN, combined biochar (7.5 t ha⁻¹) and double inhibitor application decreased NH₃ and N₂O emissions, with reductions of 24.3% and 14.6%, respectively, and reduced total N leaching by up to 45.4%. Biochar application alone or combined with double inhibitors enhanced N use efficiency from 26.2% (RN) to 44.7% (RNIB2). Conversely, double inhibitor application alone or combined with biochar enhanced rice yield and reduced yield-scaled N₂O emissions. Our results suggest that double inhibitor application alone or combined with 7.5 t ha⁻¹ biochar is an effective practice to mitigate NH₃ and N₂O emission and N leaching in paddy fields.
اظهر المزيد [+] اقل [-]Exposure duration and composition are important variables to predict short-term toxicity of effluents to a tropical copepod, Acartia sinjiensis
2022
Stone, Sarah | McKnight, Kitty S. | Naab, Christoph | Koppel, Darren J. | Binet, Monique T. | Jolley, Dianne F. | Simpson, Stuart L.
Predicting the toxicity of effluent exposures, which vary in duration, composition, and concentration, poses a challenge for ecological risk assessments. Effluent discharges may frequently result in the exposure of aquatic organisms to high concentrations of mixed contaminants for short durations. In the receiving environment effluents will undergo dilution and physical or chemical processes that further reduce contaminant concentrations at varying rates. To date, most studies comparing toxicity risks of continuous and pulsed contaminant exposures have focused on individual contaminants. In this study, the toxicity to the tropical euryhaline copepod Acartia sinjiensis of two complex effluents was assessed, comparing 6- and 18-h pulses and 78-h continuous exposures. Observations of larval development success and population size were completed after a 78-h incubation period, to observe for latent effects after pulse exposures. The chemical compositions of the effluents were assessed over time and different contaminants (i.e., metals, ammonia or organics) declined at differing rates. These were characterized as either a minimal, steady, or rapid decline. Nauplii development and population after 78 h were more impacted by effluent exposures following an 18-h pulse, compared to a 6-h pulse. Based on pulse-exposure concentrations, the 50% effect concentrations (EC50) were similar for continuous and 18-h exposures but up to 3-fold greater (lower toxicity) for the shorter 6-h exposures. Time-weighted average concentrations did not accurately predict toxicity from pulse exposures of the effluents. Concentration-addition toxicity modelling using toxicity data from pulse exposures of single contaminants was useful for predicting the toxicity of chemical mixtures exposed for varying durations. Recommendations for modified approaches to assessing risks of short-term effluent discharges are discussed.
اظهر المزيد [+] اقل [-]Contribution of liquid water content enhancing aqueous phase reaction forming ambient particulate nitrosamines
2022
Choi, Na Rae | Park, Seungshik | Ju, Seoryeong | Lim, Yong Bin | Lee, Ji Yi | Kim, Eunhye | Kim, Soontae | Shin, Hye Jung | Kim, Yong Pyo
Contribution of liquid water content (LWC) to the levels of the carcinogenic particulate nitro(so) compounds and the chemistry affecting LWC were investigated based on the observation of seven nitrosamines and two nitramines in rural (Seosan) and urban (Seoul) area in South Korea during October 2019 and a model simulation. The concentrations of both the total nitrosamines and nitramines were higher in Seosan (12.48 ± 16.12 ng/m³ and 0.65 ± 0.71 ng/m³, respectively) than Seoul (7.41 ± 13.59 ng/m³ and 0.24 ± 0.15 ng/m³, respectively). The estimated LWC using a thermodynamic model in Seosan (12.92 ± 9.77 μg/m³) was higher than that in Seoul (6.20 ± 5.35 μg/m³) mainly due to higher relative humidity (75 ± 9% (Seosan); 62 ± 10% (Seoul)) and higher concentrations of free ammonia (0.13 ± 0.09 μmol/m³ (Seosan); 0.08 ± 0.01 μmol/m³ (Seoul)) and total nitric acid (0.09 ± 0.07 μmol/m³ (Seosan); 0.04 ± 0.02 μmol/m³ (Seoul)) in Seosan while neither fog nor rain occurred during the sampling period. The relatively high concentrations of the particulate nitrosamines (>30 ng/m³) only observed probably due to the higher LWC (>10 μg/m³) in Seosan. It implies that aqueous phase reactions involving NO₂ and/or uptake from the gas phase enhanced by LWC could be promoted in Seosan. Strong correlation between the concentrations of nitrosodi-methylamine (NDMA), an example of nitrosamines, simulated by a kinetic box model including the aqueous phase reactions and the measured concentration of NDMA in Seosan (R = 0.77; 0.37 (Seoul)) indicates that the aqueous phase reactions dominantly enhanced the NDMA concentrations in Seosan. On the other hand, it is estimated that the formation of nitrosamines by aqueous phase reaction was not significant due to the relatively lower LWC in Seoul compared to that in Seosan. Furthermore, it is presumed that nitramines are mostly emitted from the primary emission sources. This study implies that the concentration of the particulate nitrosamines can be promoted by aqueous phase reaction enhanced by LWC.
اظهر المزيد [+] اقل [-]A catastrophic change in a european protected wetland: From harmful phytoplankton blooms to fish and bird kill
2022
Demertzioglou, Maria | Genitsaris, Savvas | Mazaris, Antonios D. | Kyparissis, Aris | Voutsa, Dimitra | Kozari, Argyri | Kormas, Konstantinos Ar | Stefanidou, Natassa | Katsiapi, Matina | Michaloudi, Evangelia | Moustaka-Gouni, Maria
Understanding the processes that underlay an ecological disaster represents a major scientific challenge. Here, we investigated phytoplankton and zooplankton community changes before and during a fauna mass kill in a European protected wetland. Evidence on gradual development and collapse of harmful phytoplankton blooms, allowed us to delineate the biotic and abiotic interactions that led to this ecological disaster. Before the mass fauna kill, mixed blooms of known harmful cyanobacteria and the killer alga Prymnesium parvum altered biomass flow and minimized zooplankton resource use efficiency. These blooms collapsed under high nutrient concentrations and inhibitory ammonia levels, with low phytoplankton biomass leading to a dramatic drop in photosynthetic oxygenation and a shift to a heterotrophic ecosystem phase. Along with the phytoplankton collapse, extremely high numbers of red planktonic crustaceans-Daphnia magna, visible through satellite images, indicated low oxygen conditions as well as a decrease or absence of fish predation pressure. Our findings provide clear evidence that the mass episode of fish and birds kill resulted through severe changes in phytoplankton and zooplankton dynamics, and the alternation on key abiotic conditions. Our study highlights that plankton-related ecosystem functions mirror the accumulated heavy anthropogenic impacts on freshwaters and could reflect a failure in conservation and restoration measures.
اظهر المزيد [+] اقل [-]Claroideoglomus etunicatum affects the structural and functional genes of the rhizosphere microbial community to help maize resist Cd and La stresses
2022
Hao, Baihui | Zhang, Zhechao | Bao, Zhihua | Hao, Lijun | Diao, Fengwei | Li, Frank Yonghong | Guo, Wei
Arbuscular mycorrhizal fungi (AMF) and plant rhizosphere microbes reportedly enhance plant tolerance to abiotic stresses and promote plant growth in contaminated soils. The co-contamination of soil by heavy metals (e.g., Cd) and rare earth elements (e.g., La) represents a severe environmental problem. Although the influence of AMF in the phytoremediation of contaminated soils is well documented, the underlying interactive mechanisms between AMF and rhizosphere microbes are still unclear. We conducted a greenhouse pot experiment to evaluate the effects of AMF (Claroideoglomus etunicatum) on maize growth, nutrient and metal uptake, rhizosphere microbial community, and functional genes in soils with separate and combined applications of Cd and La. The purpose of this experiment was to explore the mechanism of AMF affecting plant growth and metal uptake via interactions with rhizosphere microbes. We found that C. etunicatum (i) significantly enhanced plant nutritional level and biomass and decreased metal concentration in the co-contaminated soil; (ii) significantly altered the structure of maize rhizosphere bacterial and fungal communities; (iii) strongly enriched the abundance of carbohydrate metabolism genes, ammonia and nitrate production genes, IAA (indole-3-acetic acid) and ACC deaminase (1-aminocyclopropane-1-carboxylate) genes, and slightly altered the abundance of P-related functional genes; (iv) regulated the abundance of microbial quorum sensing system and metal membrane transporter genes, thereby improving the stability and adaptability of the rhizosphere microbial community. This study provides evidence of AMF improving plant growth and resistance to Cd and La stresses by regulating plant rhizosphere microbial communities and aids our understanding of the underlying mechanisms.
اظهر المزيد [+] اقل [-]Effect of zinc and iron oxide nanoparticles on plant physiology, seed quality and microbial community structure in a rice-soil-microbial ecosystem
2022
Afzal, Shadma | Singh, Nand K.
In this study, we assessed the impact of zinc oxide (ZnO) and iron oxide (FeO) (<36 nm) nanoparticles (NPs) as well as their sulphate salt (bulk) counterpart (0, 25, 100 mg/kg) on rice growth and seed quality as well as the microbial community in the rhizosphere environment of rice. During the rice growing season 2021–22, all experiments were conducted in a greenhouse (temperature: day 30 °C; night 20 °C; relative humidity: 70%; light period: 16 h/8 h, day/night) in rice field soil. Results showed that low concentrations of FeO and ZnO NPs (25 mg/kg) promoted rice growth (height (29%, 16%), pigment content (2%, 3%)) and grain quality parameters such as grains per spike (8%, 9%), dry weight of grains (12%, 14%) respectively. As compared to the control group, the Zn (2%) and Fe (5%) accumulations at their respective low concentrations of NP treatments showed stimulation. Interestingly, our results showed that at low concentration of both the NPs the soil microbes had more diversity and richness than those in the bulk treated and control soil group. Although a number of phyla were affected by the presence of NPs, the strongest effects were observed for change in the abundance of the three phyla for Proteobacteria, Actinobacteria, and Planctomycetes. The rhizosphere environment was notably enriched with potential streptomycin producers, carbon and nitrogen fixers, and lignin degraders with regard to functional groups of microorganisms. However, microbial communities mainly responsible for chitin degradation, ammonia oxidation, and nitrite reduction were found to be decreased. The results from this study highlight significant changes in several plant-based endpoints, as well as the rhizosphere soil microorganisms. It further adds information to our understanding of the nanoscale-specific impacts of important micronutrient oxides on both rice and its associated soil microbiome.
اظهر المزيد [+] اقل [-]Exploring use of a commercial passive sampler in a closed static chamber to measure ammonia volatilization
2022
Jaeman, Sabrina | Nurulhuda, Khairudin | Amin, Adibah Mohd | Sulaiman, Muhammad Firdaus | Man, Hasfalina Che
Studies have indicated that up to 47% of total N fertilizer applied in flooded rice fields may be lost to the atmosphere through NH₃ volatilization. The volatilized NH₃ represents monetary loss and contributes to increase in formation of PM₂.₅ in the atmosphere, eutrophication in surface water, and degrades water and soil quality. The NH₃ is also a precursor to N₂O formation. Thus, it is important to monitor NH₃ volatilization from fertilized and flooded rice fields. Commercially available samplers offer ease of transportation and installation, and thus, may be considered as NH₃ absorbents for the static chamber method. Hence, the objective of this study is to investigate the use of a commercially available NH₃ sampler/absorbent (i.e., Ogawa® passive sampler) for implementation in a static chamber. In this study, forty closed static chambers were used to study two factors (i.e., trapping methods, exposure duration) arranged in a Randomized Complete Block Design. The three trapping methods are standard boric acid solution, Ogawa® passive sampler with acid-coated pads and exposed coated pads without casing. The exposure durations are 1 and 4 h. Results suggest that different levels of absorbed NH₃ was obtained for each of the trapping methods. Highest level of NH₃ was trapped by the standard boric acid solution, followed by the exposed acid-coated pads without casing, and finally acid-coated pads with protective casing, given the same exposure duration. The differences in absorbed NH₃ under same conditions does not warrant direct comparison across the different trapping methods. Any three trapping methods can be used for conducting studies to compare multi-treatments using the static chamber method, provided the same trapping method is applied for all chambers.
اظهر المزيد [+] اقل [-]CircRNA-IGLL1/miR-15a/RNF43 axis mediates ammonia-induced autophagy in broilers jejunum via Wnt/β-catenin pathway
2022
Wang, Yue | Wang, Shengchen | Jing, Hongyuan | Zhang, Tianyi | Song, Nuan | Xu, Shiwen
With the continued increase of global ammonia emission, the damage to human or animal caused by ammonia pollution has attracted wide attention. The noncoding RNAs have been reported to regulate a variety of biological processes under different environmental stimulation via ceRNA (competing endogenous RNA) networks. Autophagy is a hallmark of tissue damage from air pollution. However, the specific role of circular RNAs (circRNAs) in the injury of intestinal tissue caused by autophagy remains unclear. Here, we established 42-days old ammonia-exposed broiler models and observed that autophagy flux in broiler jejunum was activated under ammonia exposure. Meanwhile, a total of eight significantly dysregulated expressed circRNAs were obtained and a circRNAs-miRNAs-genes interaction networks were constructed by bioinformatics analysis. Furthermore, an axis named circRNA-IGLL1/miR-15a/RNF43 was predicted to participate in the excessive autophagy by targeting RNF43. The target relationship was proved by dual-luciferase reporter assay in vitro. Mechanistically, downregulated circRNA-IGLL1 could suppress the expression of RNF43 in ammonia-exposed jejunum and the Wnt/β-catenin pathway was activated. Inhibition of miR-15a reversed autophagy caused by downregulated circRNA-IGLL1. CircRNA-IGLL1 could competitively bind miR-15a to regulate RNF43 expression, thus modulating the occurrence of autophagy. Taken together, our results showed that circRNA-IGLL1/miR-15a/RNF43 axis is involved in ammonia-induced intestinal autophagy in broilers.
اظهر المزيد [+] اقل [-]Changes of δ15N values during the volatilization process after applying urea on soil
2021
Ti, Chaopu | Ma, Shutan | Peng, Lingyun | Tao, Limin | Wang, Xi | Dong, Wenxu | Wang, Liangjie | Yan, Xiaoyuan
Ammonia (NH₃) volatilized from soils plays an important role in N cycle and air pollution, thus it is important to trace the emission source and predict source contributions to development strategies mitigating the environmental harmful of soil NH₃ volatilization. The measurements of ¹⁵N natural abundance (δ¹⁵N) could be used as a complementary tool for apportioning emissions sources to resolve the contribution of multiple NH₃ emission sources to air NH₃ pollution. However, information of the changes of δ¹⁵N–NH₃ values during the whole volatilization process under different N application rates are currently lacking. Hence, to fill this gap, we conducted a 15-day incubation experiment included different urea-N application rates to determine δ¹⁵N values of NH₃ during volatilization process. Results showed that volatilization process depleted ¹⁵N in NH₃. The average δ¹⁵N value of NH₃ volatilized from the 0, 20, 180, and 360 kg N ha⁻¹ treatment was −16.2 ± 7.3‰, −26.0 ± 5.4‰, −34.8 ± 4.8‰, and −40.6 ± 5.7‰. Overall, δ¹⁵N–NH₃ values ranged from −46.0‰ to −4.7‰ during the whole volatilization process, with lower in higher urea-N application treatments than those in control. δ¹⁵N–NH₃ values during the NH₃ volatilization process were much lower than those of the primary sources, soil (−3.4 ± 0.1‰) and urea (−3.6 ± 0.1‰). Therefore, large isotopic fractionation may occur during soil volatilization process. Moreover, negative relationships between soil NH₄⁺-N and NH₃ volatilization rate and δ¹⁵N–NH₃ values were observed in this study. Our results could be used as evidences of NH₃ source apportionments and N cycle.
اظهر المزيد [+] اقل [-]Nitrogen balance acts an indicator for estimating thresholds of nitrogen input in rice paddies of China
2021
Ding, Wencheng | Xu, Xinpeng | Zhang, Jiajia | Huang, Shaohui | He, Ping | Zhou, Wei
Decision-making related to nitrogen (N) fertilization is a crucial step in agronomic practices because of its direct interactions with agronomic productivity and environmental risk. Here, we hypothesized that soil apparent N balance could be used as an indicator to determine the thresholds of N input through analyzing the responses of the yield and N loss to N balance. Based on the observations from 951 field experiments conducted in rice (Oryza sativa L.) cropping systems of China, we established the relationships between N balance and ammonia (NH₃) volatilization, yield increase ratio, and N application rate, respectively. Dramatical increase of NH₃ volatilizations and stagnant increase of the rice yields were observed when the N surplus exceeded certain levels. Using a piecewise regression method, the seasonal upper limits of N surplus were determined as 44.3 and 90.9 kg N ha⁻¹ under straw-return and straw-removal scenarios, respectively, derived from the responses of NH₃ volatilization, and were determined as 53.0–74.9 and 97.9–112.0 kg N ha⁻¹ under straw-return and straw-removal scenarios, respectively, derived from the maximum-yield consideration. Based on the upper limits of N surplus, the thresholds of N application rate suggested to be applied in single, middle-MLYR, middle-SW, early, and late rice types ranged 179.0–214.9 kg N ha⁻¹ in order to restrict the NH₃ volatilization, and ranged 193.3–249.8 kg N ha⁻¹ in order to achieve the maximum yields. If rice straw was returned to fields, on average, the thresholds of N application rate could be theoretically decreased by 17.5 kg N ha⁻¹. This study provides a robust reference for restricting the N surplus and the synthetic fertilizer N input in rice fields, which will guide yield goals and environmental protection.
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