Plants detoxify toxic metal(loid)s by accumulating diverse metabolites. Beside scavenging excess reactive oxygen species (ROS) induced by metal(loid)s, some metabolites chelate metal(loid) ions. Classically, thiol-containing compounds, especially glutathione (GSH) and phytochelatins (PCs) are thought to be the major chelators that conjugate with metal(loid)s in the cytoplasm followed by transport and sequestration in the vacuole. In addition to this classical detoxification pathway, a role for secondary metabolites in metal(loid) detoxification has recently emerged. In particular, anthocyanins, a kind of flavonoids with ROS scavenging potential, contribute to enhanced arsenic tolerance in several plant species. Evidence is accumulating that, in analogy to GSH and PCs, anthocyanins may conjugate with arsenic followed by vacuolar sequestration in the detoxification event. Exogenous application or endogenous accumulation of anthocyanins enhances arsenic tolerance, leading to improved plant growth and productivity. The application of some plant hormones and signaling molecules stimulates endogenous anthocyanin synthesis which confers tolerance to arsenic stress. Anthocyanin biosynthesis is transcriptionally regulated by several transcription factors, including myeloblastosis (MYBs). The light-regulated transcription factor elongated hypocotyl 5 (HY5) also affects anthocyanin biosynthesis, but its role in arsenic tolerance remains elusive. Here, we review the mechanism of arsenic detoxification in plants and the potential role of anthocyanins in arsenic tolerance beyond the classical points of view. Our analysis proposes that anthocyanin manipulation in crop plants may ensure sustainable crop yield and food safety in the marginal lands prone to arsenic pollution.

The present study investigated that the potential of soil or foliar applied 15 mg/L zinc oxide quantum dots (ZnO QD, 11.7 nm) to enhance pumpkin (Cucurbita moschata Duch.) growth and biomass in comparison with the equivalent concentrations of other sizes of ZnO particles, ZnO nanoparticles (ZnO NPs, 43.3 nm) and ZnO bulk particles (ZnO BPs, 496.7 nm). In addition, ZnSO4 was used to set a Zn²⁺ ionic control. For foliar exposure, ZnO QD increased dry mass by 56% relative to the controls and values were 17.3% greater than that of the ZnO NPs particles. The cumulative water loss in the ZnO QD treatment was 10% greater than with ZnO NPs, suggesting that QD could better enhance pumpkin growth. For the root exposure, biomass and accumulative water loss equivalent across all Zn treatments. No adverse effects in terms of pigment (chlorophyll and anthocyanin) contents were evident across all Zn types regardless exposure routes. Foliar exposure to ZnO QD caused 40% increases in shoot Zn content as compared to the control; the highest Zn content was evident in the Zn²⁺ ionic treatment, although this did not lead to growth enhancement. In addition, the shoot and root content of other macro- and micro-nutrients were largely equivalent across all the treatments. The contents of other nutritional compounds, including amino acids, total protein and sugar, were also significantly increased by foliar exposure of ZnO QD. The total protein in the ZnO QD was 53% higher than the ZnO particle treatments in the root exposure group. Taken together, our findings suggest that ZnO QDs have significant potential as a novel and sustainable nano-enabled agrichemical and strategies should be developed to optimize benefit conferred to amended crops.

Increasing attention has been brought to microplastics pollution recently, while emerging evidences indicate that nano-plastics degraded from microplastics are more of research significance owing to stronger toxicity. However, there is little study focused on the prevention of nano-plastics induced toxicity until now. Canidin-3-glucoside (C3G), a natural anthocyanin proved to possess multiple functions like antioxidant and intestinal tissue protection. Thus, we proposed whether C3G could act as a molecular weapon against nano-plastics induced toxicity. In Caco2 cell and Caenorhabditis elegans (C. elegans) models, we found that polystyrene (PS) nano-plastics exposure resulted in physiological toxicity and oxidative damage, which could be restored by C3G. More significantly in Caco2 cells, we observed that autophagy was activated via Sirt1-Foxo1 signaling pathway to attenuate PS induced toxicity after C3G intervention and further verified by adding autophagy inhibitor 3-Methyladenine (3-MA). Meanwhile, PS co-localization with lysosomes was observed, indicating the encapsulation and degradation of PS. In C. elegans, by detecting LGG-1/LC3 expression in GFP-targeted LGG-1 report gene (LGG-1:GFP) labeled transgenic DA2123 strain, the co-localization of LGG-1:GFP with PS was found as well, means that autophagy is involved in C3G’s beneficial effects. Furthermore, we were surprised to find that C3G could promote the discharge of PS from N2 nematodes, which reduces PS toxicity more directly.

Perfluorooctanoic acid (PFOA) is a widely used synthetic industrial chemical which accumulates in ecosystems and organisms. Our study have investigated the neurobehavioral effects of PFOA and the alleviation effects of PFOA-induced neurotoxicity by blueberry anthocyanins (ANT) in Dugesia japonica. The planarians were exposed to PFOA and ANT for ten days. Researchs showed that exposure to PFOA affected locomotor behavior and ANT significantly alleviated the reduction in locomotion induced by PFOA. The regeneration of eyespots and auricles was suppressed by PFOA and was promoted by ANT. Following exposure to PFOA, acetylcholinesterase activity continually decreased and was unaffected in the ANT group, but was elevated after combined administration of PFOA and ANT. Oxidative DNA damage was found in planarians exposed to PFOA and was attenuated after administration of ANT by the alkaline comet assay. Concentrations of three neurotransmitters increased following exposure to PFOA and decreased after administration of ANT. Furthermore, ANT promoted and PFOA inhibited neuronal regeneration. DjotxA, DjotxB, DjFoxG, DjFoxD and Djnlg associated with neural processes were up-regulated following exposure to PFOA. Our findings indicate that PFOA is a neurotoxicant while ANT can attenuate these detrimental effects.

Blueberry anthocyanin (BA) have strong health benefits as an active natural antioxidant and perfluorooctanoic acid (PFOA) can result in oxidative stress in animals. In our study, the protective effects of BA against stress induced by PFOA was investigated in the planarian Dugesia japonica using oxidative stress biomarkers, ATP contents, ATPase activity, DNA methylation and mRNA expression. PFOA exposure could resulted in malondialdehyde production. At the same time, treatment with BA decreased the production of malondialdehyde in BA-exposed and co-treatment planarians. PFOA caused activities increase in glutathione peroxidase (GPx), glutathione S-transferase (GST) and activities decrease in glutathione reductase (GR). PFOA exposure decreased the GSH and ATP contents. Additionally, it increased the GSSG contents and ATPase activity. BA administration increased the activities of GPx, GST and GR in BA and co-treatment planarians. Meanwhile BA maintained the contents of ATP, ATPase activity, GSH and GSSG by alleviating PFOA toxicity. Moreover, PFOA and BA increased the contents of 5-methylcytosine and decreased 5-hydroxymethylcytosine in all group. In addition, PFOA and BA treated planarians significantly altered the expression of genes associated with above biochemical parameters. The results showed that the mRNA expression of gpx, Djgst, gr, Djnak and dnmt1 were significantly elevated in all groups. Alterations in the mRNA expression levels indicated a stress response to PFOA exposure and anthocyanin protection. These alterations regulated biomarkers of oxidative stress, energy metabolism and DNA methylation levels in planarians. These results indicate that BA attenuated PFOA-induced oxidative stress, energy metabolism, DNA methylation and gene expression disorders.

In this study, we investigated the physiological and photochemical influences of nanoTiO2 exposure on tomato plants (Lycopersicum esculentum Mill.). Tomato plants were exposed to 100 mg L−1 of nanoTiO2 for 90 days in a hydroponic system. Light irradiances of 135 and 550 μmolphoton m−2 s−1 were applied as environmental stressors that could affect uptake of nanoTiO2. To quantify nanoTiO2 accumulation in plant bodies and roots, we used transmission electron microscopy, energy-dispersive X-ray spectroscopy, inductively coupled plasma mass spectrometry, and X-ray powder diffraction. Phenotypic and physiological influences such as color change, growth rate, fruit productivity, pigment concentration, and enzyme activity (SOD, CAT, APX) were monitored. We observed numerous effects caused by high irradiance and nanoTiO2 exposure, such as rapid chlorophyll decrease, increased anthocyanin and carotenoid concentrations, high enzymatic activity, and an approximately eight-fold increase in fruit production. Moreover, light absorption in the nanoTiO2-treated tomato plants, as measured by a ultraviolet–visible light spectrometer, increased by a factor of approximately 19, likely due to natural pigments that worked as sensitizers, and this resulted in an ∼120% increase in photochemical activities on A, ФPSII, ФCO2, gsw, and E.

The present study aimed to show that nickel and fluoride exhibited synchronized co-inhibited uptake in the aromatic rice cultivar, Gobindobhog, since bioaccumulation of the two elements was lower than that during individual stress, so that overall growth under combined stress was similar to control seedlings. On the contrary, lead and fluoride stimulated their co-uptake which triggered oxidative damages, NADPH oxidase activity, methylglyoxal accumulation, photosynthetic inhibition, membrane-protein damages, necrosis and genomic template degradation. Accumulation of proline, anthocyanins, non-protein thiols and phytochelatins was stimulated for systemic protection against reactive oxygen species (ROS) and xenobiotic-mediated injuries during lead-fluoride toxicity. ROS accumulation during nickel-fluoride stress was insignificant due to which enhanced accumulation of most antioxidants was not required. Glutathione depletion during combined lead-fluoride toxicity was due to its utilization in the glyoxalase cycle and also inhibition of glutathione reductase. However, the nickel-fluoride-treated sets maintained glutathione reserves and glyoxalase activity similar to those in control. Presence of fluoride ‘safeguarded’ the glutathione-utilizing enzymes like glutathione reductase, glutathione peroxidase and glutathione-S-transferase during dual lead-fluoride stress. This was because these enzymes showed higher activity compared to that under lead toxicity alone. Enzymatic antioxidants like superoxide dismutase, ascorbate peroxidase and guaiacol peroxidase were activated during lead-fluoride toxicity due to altered iron and copper homeostasis. Catalase activity was strongly inhibited, resulting in the inability to scavenge H₂O₂ and suppression of the fluoride-adaptable phenotype. However, none of the enzymatic antioxidants were inhibited during nickel-fluoride stress, which cumulatively allowed the seedlings to maintain normal physiology. Overall our findings holistically reveal the physiological plasticity of Gobindobhog in response to two different heavy metals under the influence of fluoride.

Sea grasses are foundation species for estuarine ecosystems. The available light for sea grasses diminishes rapidly during pollutant spills, effluent releases, disturbances such as intense riverine input, and tidal changes. We studied how sea grasses’ remote-sensing signatures and light-capturing ability respond to short term light alterations. In vivo responses were measured over the entire visible-light spectra to diminishing white-light on whole-living-plants’ spectral reflectance, including 6h of full oceanic-light fluences from 10% to 100%. We analyzed differences by various reflectance indices. We compared the sea grasses species responses of tropical vs. temperate and intertidals (Halodule wrightii, and Zostera marina) vs. subtidal (Thalassia testudinum). Reflectance diminished with decreasing light intensity that coincided with greater accessory pigment stimulation (anthocyanin, carotenoids, xanthins). Chlorophyll a and Chlorophyll b differed significantly among species (Thalassia vs. Halodule). Photosynthetic efficiency diminished at high light intensities. The NDVI index was inadequate to perceive these differences. Our results demonstrate the leaf-level utility of data to remote sensing for mapping sea grass and sea grass stress.

Rising tropospheric ozone concentrations can cause rice yield losses and necessitate the breeding of ozone-tolerant rice varieties. However, ozone tolerance should not compromise the resistance to important biotic stresses such as the rice blast disease. Therefore, we investigated the interactive effects of ozone and rice blast disease on nine different rice varieties in an experiment testing an ozone treatment, blast inoculation, and their interaction. Plants were exposed to an ozone concentration of 100 ppb for 7 h per day or ambient air throughout the growth period. Half of the plants were simultaneously infected with rice blast inoculum. Grain yield was significantly reduced in the blast treatment (17%) and ozone treatment (37%), while the combination of both stresses did not further decrease grain yields compared to ozone alone. Similar trends occurred for physiological traits such as vegetation indices, normalized difference vegetation index (NDVI), photochemical reflectance index (PRI), Lichtenthaler index 2 (Lic2), and anthocyanin reflectance index 1 (ARI1), as well as stomatal conductance and lipid peroxidation. Ozone exposure mitigated the formation of visible blast symptoms, while blast inoculation did not significantly affect visible ozone symptoms. Although different genotypes showed contrasting responses to the two types of stresses, no systematic pattern was observed regarding synergies or trade-offs under the two types of stresses. Therefore, we conclude that despite the similarities in physiological stress responses to ozone and blast, the tolerance to these stresses does not appear to be genetically linked in rice.

Nanoparticles (NPs) are an emerging tool for mitigating environmental stresses. Although beneficial roles of NPs have been reported in some plants, there is little data on magnesium (Mg)-NPs in alleviating drought stress. Therefore, the field experiment was conducted to study changes in biochemical attributes and essential oil (EO) compositions of yarrow (Achillea millefolium L.) plants under drought stress and Mg-NPs in 2016 and 2017. Irrigation regimes were used in two levels as well-watered (irrigation intervals of 7 days) and drought stress (irrigation intervals of 14 days) conditions, and Mg-NPs were sprayed on leaves in four levels (0, 0.1, 0.3, and 0.5 g L⁻¹). The results showed drought stress led to increased electrolyte leakage (EL), proline, carotenoid, anthocyanin, and total flavonoid content (TFC). However, flowers yield and EO yield were lower in plants exposed to drought stress as compared to well-watered conditions. The 0.3 and 0.5 g L⁻¹ Mg-NPs were more effective in alleviating drought stress by enhancing these traits. Heat map results showed that EL and TSS represented the high variability upon different treatments. The GC and GC/MS results represented that α-pinene (8.60–12.20%), 1,8-cineol (9.03–14.02%), camphor (6.84–9.80%), α-bisabolol (8.54–18.81%), chamazulene (14.23–22.50%), and caryophyllene oxide (7.20–9.80%) were the min EO constitutes of yarrow plants. Totally, drought decreased monopertens but increased sesquiterpenes of EO. To sum up, foliar applied Mg-NPs in a range of 0.3–0.5 g L⁻¹ can be recommended as effective tool to improve plant yield through changes in biochemical attributes of yarrow plants.