The aim of this study was to assess the impact of treated wastewaters on native wild Prussian carp inhabiting effluent-receiving waters (ERC) receiving municipal and sugar plant treated wastewaters, further downstream waters (DW), and a detached canal unaffected by the WWTP activities. To that end, general fish health status was determined, including plasma biochemical, haematological, oxidative stress and tissue histopathological indices, over three seasons. The greatest tissue alterations were in fall in ERC during sugar beet processing, as hypertrophy of gill epithelial and interlamellar cells, necrosis and lymphocytic infiltration, hyperplasia and hypertrophy of renal tubules, distention of hepatic sinusoids. In fall the lowest leukocytes, lymphocytes and granulocytes (2467 ± 565, 1333 ± 264, 1133 ± 488 cells/μL respectively), as well as highest plasma ALP (52.7 ± 19.39 U/L) were measured. ERC in fall had the highest ammonium (20 mg/L), nitrite (1.48 mg/L), nitrate (13.4 mg/L), and lowest dissolved O2 (1.23 mg/L). Gill, kidney and liver alterations, and the highest plasma cholesterol (9.1 ± 1.98 mmol/L) were noted in DW fish in fall. Tissue morphology during sugar cane processing seems a consequence of cellular and structural tissue integrity loss. Structural heterogeneity of gills and spleen was enhanced with increasing concentrations of heavy metals and correlated with oxidative stress (SOD 392.5 ± 77.28 U/L). Monogenean infestation was moderate in ERC fish in all seasons compared with DW fish. Prussian carp biological responses to multiple stressors, measured by the effects of WWTP on blood and tissue parameters, reached far downstream and were not of localized nature. This study demonstrated that in aquatic environments impacted with complex contaminants acting synergistically, causal relationships between biological responses and environmental stressors should be interpreted. Integrated histopathological, haematological and biochemical findings are valuable biomarkers for native fish adaptive patterns and monitoring of water quality/pollution of freshwater ecosystems.

Soil salinity adversely affects the growth, yield, and quality parameters of sugar beet, leading to a reduction in root and sugar yields. Improving the physical and chemical properties of salt-affected soils is essential for sustainable cultivation and sugar beet production. A field experiment was conducted at the Delta Sugar Company Research Farm, El-Hamool, Kafr El-Sheikh, Egypt, to evaluate the response of sugar beet to the application of beet sugar filter cake treated with sulfuric and phosphoric acid-treated, phosphogypsum (PG), desaline, humic acid, and molasses under saline soil conditions. The application of treated filter cake enhanced root length, diameter, and leaf area. The application of molasses enhanced root length, diameter, and leaf area as well. Application of molasses increased sugar content and root yield. The application of either treated filter cake or molasses produced the highest recoverable sugar yield. Linear regression analysis revealed that the root yield, quality index, and recoverable sugar yield increased in response to the increased availability of either Ca²⁺ or K content in the soil which increases in response to the application of soil amendments and molasses. The application of treated beet sugar filter cake and molasses increased the calcium, magnesium, and potassium availability in the soil. Treated filter cake is a promising organic soil amendment that enhanced the yield by 29% and yield-related traits of sugar beet by improving the physical and chemical properties of the soil.

Improving the chemical and physical properties of saline soils is crucial for the sustainable production of sugar beet and efficient processing of beet sugar. Here, the impacts of the application of treated filter cake on sugar beet biofortification under saline soil and sugar losses into molasses during beet sugar processing were evaluated for the first time. The application of treated filter cake significantly reduced K%, Na%, and α-amino-N while enhanced sucrose content and quality index of beet root juice. Consequently, sugar loss percentage, sugar loss yield, and relative sugar loss yield were reduced, whereas recoverable sugar yield was enhanced. Linear regression analysis revealed that quality index and sugar loss yield were increased, whereas sugar loss percentage and relative sugar loss yield were reduced in response to the reduction of soil Na⁺ content accompanied with increasing Ca²⁺ content in the soil increased. The results provide treated filter cake as a promising amendment for saline soil remediation for improving biofortification of sugar beet and reducing sugar losses during beet sugar processing.