خيارات البحث
النتائج 1 - 10 من 93
Characterization and biodegradability assessment of water-soluble fraction of oily sludge using stir bar sorptive extraction and GCxGC-TOF MS
2022
Chand, Priyankar | Dutta, Suryendu | Mukherji, Suparna
Percolation of water through oily sludge during storage and handling of the sludge can cause soil and groundwater contamination. In this study, oily sludge from a refinery was equilibrated with water to obtain the water-soluble fraction (WSF) of oily sludge. The WSF had dissolved organic carbon (DOC) of 166 mg/L. Human cell line-based toxicity assay revealed IC₅₀ of 41 mg/L indicating its toxic nature. The predominant compounds in WSF of oily sludge included isomers of methyl, dimethyl and trimethyl quinolines and naphthalenes along with phenol derivatives and other polynuclear aromatic hydrocarbons (PAHs). Biodegradation of WSF of oily sludge was studied using a consortium of Rhodococcus ruber, Bacillus sp. and Bacillus cereus isolated from the refinery sludge. The consortium of the three strains resulted in 70% degradation over 15 days with a first-order degradation rate of 0.161 day⁻¹. Further analysis of the WSF was performed using the stir-bar sorptive extraction (SBSE) followed by GCxGC-TOF MS employing a PDMS Twister. The GCxGC analysis showed that Bacillus cereus was capable of degrading the quinoline, phenol and naphthalene derivatives in WSF of oily sludge at a faster rate compared to pyridine and benzoquinoline derivatives. Quinoline, phenol, biphenyl, naphthalene, pyridine and benzoquinolines derivatives in the WSF of oily sludge were reduced by 87%, 92%, 88%, 77%, 40% and 62%, respectively with respect to the controls. The WSF of oily sludge contained, n-alkanes, ranging from n-C12 to n-C18 which were removed within 2 days of biodegradation.
اظهر المزيد [+] اقل [-]Phase-specific stable isotope fractionation effects during combined gas-liquid phase exchange and biodegradation
2022
Khan, Ali M. | Gharasoo, Mehdi | Wick, Lukas Y. | Thullner, Martin
Stable isotope fractionation of toluene under dynamic phase exchange was studied aiming at ascertaining the effects of gas-liquid partitioning and biodegradation of toluene stable isotope composition in liquid-air phase exchange reactors (Laper). The liquid phase consisted of a mixture of aqueous minimal media, a known amount of a mixture of deuterated (toluene-d) and non-deuterated toluene (toluene-h), and bacteria of toluene degrading strain Pseudomonas putida KT2442. During biodegradation experiments, the liquid and air-phase concentrations of both toluene isotopologues were monitored to determine the observable stable isotope fractionation in each phase. The results show a strong fractionation in both phases with apparent enrichment factors beyond −800‰. An offset was observed between enrichment factors in the liquid and the gas phase with gas-phase values showing a stronger fractionation in the gas than in the liquid phase. Numerical simulation and parameter fitting routine was used to challenge hypotheses to explain the unexpected experimental data. The numerical results showed that either a very strong, yet unlikely, fractionation of the phase exchange process or a – so far unreported – direct consumption of gas phase compounds by aqueous phase microorganisms could explain the observed fractionation effects. The observed effect can be of relevance for the analysis of volatile contaminant biodegradation using stable isotope analysis in unsaturated subsurface compartments or other environmental compartment containing a gas and a liquid phase.
اظهر المزيد [+] اقل [-]Effects of long-term exposure to the herbicide nicosulfuron on the bacterial community structure in a factory field
2022
Ma, Qingyun | Tan, Hao | Song, Jinlong | Li, Miaomiao | Wang, Zhiye | Parales, Rebecca E. | Li, Lin | Ruan, Zhiyong
This study aims to investigate the effects of long-term nicosulfuron residue on an herbicide factory ecosystem. High-throughput sequencing was used to investigate the environmental microbial community structure and interactions. The results showed that the main contributor to the differences in the microbial community structure was the sample type, followed by oxygen content, pH and nicosulfuron residue concentration. Regardless of the presence or absence of nicosulfuron, soil, sludge, and sewage were dominated by groups of Bacteroidetes, Actinobacteria, and Proteobacteria. Long-term exposure to nicosulfuron increased alpha diversity of bacteria and archaea but significantly decreased the abundance of Bacteroidetes and Acidobateria compared to soils without nicosulfuron residue. A total of 81 possible nicosulfuron-degrading bacterial genera, e.g., Rhodococcus, Chryseobacterium, Thermomonas, Stenotrophomonas, and Bacillus, were isolated from the nicosulfuron factory environmental samples through culturomics. The co-occurrence network analysis indicated that the keystone taxa were Rhodococcus, Stenotrophomonas, Nitrospira, Terrimonas, and Nitrosomonadaceae_MND1. The strong ecological relationship between microorganisms with the same network module was related to anaerobic respiration, the carbon and nitrogen cycle, and the degradation of environmental contaminants. Synthetic community (SynCom), which provides an effective top-down approach for the critical degradation strains obtained, enhanced the degradation efficiency of nicosulfuron. The results indicated that Rhodococcus sp. was the key genus in the environment of long-term nicosulfuron exposure.
اظهر المزيد [+] اقل [-]Plant-derived saponin enhances biodegradation of petroleum hydrocarbons in the rhizosphere of native wild plants
2022
Hoang, Son A. | Lamb, Dane | Sarkar, Binoy | Seshadri, Balaji | Lam, Su Shiung | Vinu, Ajayan | Bolan, Nanthi S.
Plant-derived saponins are bioactive surfactant compounds that can solubilize organic pollutants in environmental matrices, thereby facilitating pollutant remediation. Externally applied saponin has potential to enhance total petroleum hydrocarbon (TPH) biodegradation in the root zone (rhizosphere) of wild plants, but the associated mechanisms are not well understood. For the first time, this study evaluated a triterpenoid saponin (from red ash leaves, Alphitonia excelsa) in comparison to a synthetic surfactant (Triton X-100) for their effects on plant growth and biodegradation of TPH in the rhizosphere of two native wild species (a grass, Chloris truncata, and a shrub, Hakea prostrata). The addition of Triton X-100 at the highest level (1000 mg/kg) in the polluted soil significantly hindered the plant growth (reduced plant biomass and photosynthesis) and associated rhizosphere microbial activity in both the studied plants. Therefore, TPH removal in the rhizosphere of both plant species treated with the synthetic surfactant was not enhanced (at the lower level, 500 mg/kg soil) and even slightly decreased (at the highest level) compared to that in the surfactant-free (control) treatment. By contrast, TPH removal was significantly increased with saponin application (up to 60% in C. truncata at 1000 mg/kg due to enhanced plant growth and associated rhizosphere microbial activity). No significant difference was observed between the two saponin application levels. Dehydrogenase activity positively correlated with TPH removal (p < 0.001) and thus this parameter could be used as an indicator to predict the rhizoremediation efficiency. This work indicates that saponin-amended rhizoremediation could be an environmentally friendly and effective biological approach to remediate TPH-polluted soils. It was clear that the enhanced plant growth and rhizosphere microbial activity played a crucial role in TPH rhizoremediation efficiency. The saponin-induced molecular processes that promoted plant growth and soil microbial activity in the rhizosphere warrant further studies.
اظهر المزيد [+] اقل [-]Single-cell-level microfluidics assisted with resuscitation-promoting factor technology (SMART) to isolate novel biphenyl-degrading bacteria from typical soils in eastern China
2022
Jia, Yangyang | Li, Xinyi | Xu, Fengjun | Liu, Zefan | Fu, Yulong | Xu, Xin | Yang, Jiawen | Zhang, Shuai | Shen, Chaofeng
Soil microorganisms represent one of the largest biodiversity reservoirs. However, most low-abundance, slow-growing or dormant microorganisms in soils are difficult to capture with traditional enrichment culture methods. These types of microorganisms represent a valuable “microbial seed bank”. To better exploit and utilize this “microbial dark matter”, we developed a novel strategy that integrates single-cell-level isolation with microfluidics technology and culture with resuscitation-promoting factor (Rpf) to isolate biphenyl-degrading bacteria from four typical soils (paddy soil, red soil, alluvial soil and black soil) in eastern China. Multitudinous bacteria were successfully isolated and cultured; some of the identified clades have not been previously linked to biphenyl biodegradation, such as Actinotalea, Curtobacterium and Rothia. Soil microcosmic experiments validated that some bacteria are responsible for biphenyl degradation in soil. In addition, genomic sequencing and Illumina MiSeq sequencing of 16S rRNA genes indicated that exogenous Rpf mainly promotes the recovery and growth of bacteria containing endogenous Rpf-encoding genes. In summary, this study provides a novel strategy for capturing target functional microorganisms in soils, indicates potential bioresources for the bioremediation of contaminated soils, and enhances our current understanding of the mechanisms involved in the response to exogenous Rpf.
اظهر المزيد [+] اقل [-]The efficient persistence and migration of Cupriavidus gilardii T1 contribute to the removal of MCPA in laboratory and field soils
2022
Pan, Dandan | Xu, Yue | Ni, Yaxin | Zhang, Houpu | Hua, Rimao | Wu, Xiangwei
The application of exogenous biodegradation strains in pesticide-polluted soils encounters the challenges of migration and persistence of inoculants. In this study, the degradation characteristics, vertical migration capacity, and microbial ecological risk assessment of an enhanced green fluorescent protein (EGFP)-tagged 2-Methyl-4-chlorophenoxyacetic acid (MCPA)-degrading strain Cupriavidus gilardii T1 (EGFP) were investigated in the laboratory and field soils. The optimum remediation conditions for T1 (EGFP) was characterized in soils. Meanwhile, leaching experiments showed that T1 (EGFP) migrated vertically downwards in soil and contribute to the degradation of MCPA at different depths. After inoculation with T1 (EGFP), a high expression levels of EGFP gene was observed at 28 d in the laboratory soil and at 45 d in the field soil. The degradation rates of MCPA were ≥ 60% in the laboratory soil and ≥ 48% in the field soil, indicating that T1 (EGFP) can efficiently and continuously remove MCPA in both laboratory and field conditions. In addition, the inoculation of T1 (EGFP) not only showed no significant impact on the soil microbial community structure but also can alleviate the negative effects induced by MCPA to some extent. Overall, our findings suggested that T1 (EGFP) strain is an ecologically safe resource for the in situ bioremediation of MCPA-contaminated soils.
اظهر المزيد [+] اقل [-]Current status of microbes involved in the degradation of pharmaceutical and personal care products (PPCPs) pollutants in the aquatic ecosystem
2022
Narayanan, Mathiyazhagan | El-Sheekh, Mostafa | Ma, Ying | Pugazhendhi, Arivalagan | Natarajan, Devarajan | Kandasamy, Gajendiran | Raja, Rathinam | Saravana Kumar, R.M. | Kumarasamy, Suresh | Sathiyan, Govindasamy | Geetha, R. | Paulraj, Balaji | Liu, Guanglong | Kandasamy, Sabariswaran
Contamination of aquatic systems with pharmaceuticals, personal care products, steroid hormones, and agrochemicals has been an immense problem for the earth's ecosystem and health impacts. The environmental issues of well-known persistence pollutants, their metabolites, and other micro-pollutants in diverse aquatic systems around the world were collated and exposed in this review assessment. Waste Water Treatment Plant (WWTP) influents and effluents, as well as industrial, hospital, and residential effluents, include detectable concentrations of known and undiscovered persistence pollutants and metabolites. These components have been found in surface water, groundwater, drinking water, and natural water reservoirs receiving treated and untreated effluents. Several studies have found that these persistence pollutants, and also similar recalcitrant pollutants, are hazardous to a variety of non-targeted creatures in the environment. In human and animals, they can also have severe and persistent harmful consequences. Because these pollutants are harmful to aquatic organisms, microbial degradation of these persistence pollutants had the least efficiency. Fortunately, only a few wild and Genetically Modified (GMOs) microbial species have the ability to degrade these PPCPs contaminants. Hence, researchers have been studying the degradation competence of microbial communities in persistence pollutants of Pharmaceutical and Personal Care Products (PPCPs) and respective metabolites for decades, as well as possible degradation processes in various aquatic systems. As a result, this review provides comprehensive information about environmental issues and the degradation of PPCPs and their metabolites, as well as other micro-pollutants, in aquatic systems.
اظهر المزيد [+] اقل [-]Detection of Neonicotinoids in agriculture soil and degradation of thiacloprid through photo degradation, biodegradation and photo-biodegradation
2022
Elumalai, Punniyakotti | Yi, Xiaohui | Chen, Zhenguo | Rajasekar, Aruliah | Brazil de Paiva, Teresa Cristina | Hassaan, Mohamed A. | Ying, Guang-guo | Huang, Mingzhi
The social and ecological influence of Neonicotinoids (NEOs) usage in agriculture sector is progressively higher. There are seven NEOs insecticides widely used for the insects control. Among the NEOs, thiacloprid (THD) was extensively used for insect control during crop cultivation. This study targets to analyse the contamination levels of NEOs in agricultural soil and identify photo-biodegradation of THD degradation using pure isolates and mixed consortium. The photo degradation (PD), biodegradation (BD) and photo-biodegradation (PBD) of THD were compared. The corn field agricultural soils were polluted by four NEOs, among them THD had greater contamination level (surface soil: 3901.2 ± 0.04 μg/g) and (sub-surface soil: 3988.6 ± 0.05 μg/g). Three soil free enriched bacterial strains following Bacillus atrophaeus (PB-2), Priestia megaterium (PB-3) (formerly known as Bacillus megaterium), and Peribacillus simplex (PB-4) (formerly known as Bacillus simplex) were identified by microbiological and molecular 16s rRNA gene sequencing. The PD, BD and PBD of THD were conducted and degradation rate was detected by instrument UPLC-MS-MS. The PBD process with blue-LEDs showed better THD degradation efficiency than PD and BD, where the specific THD degradation rate was 85 ± 0.2%, 87 ± 0.5%, and 89 ± 0.3%, respectively for PB-2, PB-3 and PB-4. Then, the photo-biodegradation performance is greater at 150, 175, 200 rpm, pH 7.0–9.0, and temperature 30–35 °C. After the PBD system deliver four intermediate metabolites, the THD degradation process maybe through nitro reduction, hydroxylation and oxidative cleavage pathway.
اظهر المزيد [+] اقل [-]Microbial processing of autochthonous organic matter controls the biodegradation of 17α-ethinylestradiol in lake sediments under anoxic conditions
2022
Bai, Leilei | Liu, Xin | Hua, Ke | Tian, Linqi | Wang, Changhui | Jiang, Helong
The decay of algal biomass and aquatic plants in freshwater lakes leads to the overproduction of autochthonous organic matter (OM) and the exhaustion of dissolved oxygen, impacting the microbial community and subsequent biodegradation of emerging contaminants in sediment. This study explored how the microbial processing of aquatic plant- and algal-derived OM (POM and AOM) mediates 17α-ethinylestradiol (EE2) biodegradation in the anoxic sediments of Lake Taihu in China. In four months of microcosm incubations, the increased concentrations of protein-like substances in AOM and POM exhibited temporary activation on microbial metabolic enzyme activity (fluorescein diacetate hydrolase and dehydrogenase) and significantly promoted the carbon mineralization with iron reduction (P < 0.001). These in turn increased the EE2 biodegradation efficiency to 77–90 ng g⁻¹ in the anoxic sediment. However, a higher EE2 biodegradation of 109 ng g⁻¹ was achieved with the humic acid augmentation containing more quinone-like compounds, showing a weaker substrate-priming effect but accelerated redox cycling of iron and organic substrates in the later period of incubation. The microbial analysis further revealed that the quinone-like compounds in OM were more closely associated with microbial electron transfer and strengthened their interspecies syntrophic cooperation favorable to contaminant biodegradation, even though the connective members exposed to protein-like components upregulated more functional genes related to organic carbon and xenobiotics metabolism and biodegradation. Our findings will help predict the fate of estrogens in various sedimentary environments under increasing eutrophication and further climate change scenarios.
اظهر المزيد [+] اقل [-]Combined maize straw-biochar and oxalic acids induced a relay activity of abundant specific degraders for efficient phenanthrene degradation: Evidence based on the DNA-SIP technology
2022
Li, Xiaona | Yao, Shi | Bolan, Nanthi | Wang, Zhenyu | Jiang, Xin | Song, Yang
Biochar-oxalic acid composite application (BCOA) have shown to be efficient in the remediation of polycyclic aromatic hydrocarbon (PAH)-contaminated soil, but the functional degraders and the mechanism of improving biodegradation remains unclear. In this study, with the help of stable isotope probing technology of phenanthrene (Phe), we determined that BCOA significantly improved Phe mineralization by 2.1 times, which was ascribed to the increased numbers and abundances of functional degraders. The BCOA increased contents of dissolved organic carbon and available nutrients and decreased pH values in soil, thus promoting the activity, diversity and close cooperation of the functional Phe-degraders, and stimulating their functions associated with Phe degradation. In addition, there is a relay activity among more and diverse functional Phe-degraders in the soil with BCOA. Specifically, Pullulanibacillus persistently participated in Phe-degradation in the soil with BCOA throughout the incubation period. Moreover, Pullulanibacillus, Blastococcus, Alsobacter, Ramlibacter, and Mizugakiibacter were proved to be potential Phe-degraders in soil for the first time. The specific Phe degraders and their relay and cooperation activity in soils as impacted by BCOA were first identified with DNA-stable isotope probing technology. Our findings provided a novel perspective to understand the efficient degradation of PAH in the BCOA treatments, revealed the potential of soil native microbes in the efficient bioremediation of PAH-contaminated natural soil, and provided a basis for the development of in-situ phytoremediation technologies to remediate PAH pollution in future.
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