The surface water chemistry of Thiruvananthapuram Museum Lake was carried out in the period of February 2013 to January 2014. Correlation study of the parameters and overall CCME WQI (Canadian Council of Ministers of the Environment Water quality Index) was also prepared in the study for the lake water. The parameters analysed are cations such as Ca2+, Na+, Mg2+ and K+ and the anions like PO4-, Si4- , NO3- and NO2-. Abiotic factors like water pH, Temperature, Conductivity, TDS, Total Alkalinity and Total Hardness were also analysed. The pH ranged between 6.5 to 7.4. The total hardness ranged between 50.8-99 mg/L which shows the water is moderately hard one. During the pre monsoon period, water temperature showed a positive correlation with total alkalinity (r= 0.915) pH (r= 0.841) and TDS (0.876). Dissolved Oxygen (DO) value showed a positive correlation with Biological Oxygen Demand (BOD) (r= 0.999). The overall CCME water quality index was 80.81 which indicate the water body is protected with only a minor degree of threats. The quality of the water is an essential element of the Thiruvananthapuram Zoo environment with respect to both healths of the ecosystem and zoo tourism enjoyment. Moreover, if maintained properly, this water body can be treated as a major drinking water source for the zoo animals.