The method of air pollution level evaluation of urban landscapes on the basis of D. melanogaster cohort analysis has been suggested. The method implies the binding to the landscape areas of the city. Within each landscape area traps and cultivators for D. melanogaster have been installed in sanitary-protective zones of various enterprises as well as on the background territory with the least level of anthropogenic load serving as the control. Based on specifically elaborated technique for field conditions, the amount of eggs, third instar larvae, pupae and imago has been calculated. Then, using the computer program ImageJ, the square under the curves of cohort survival has been determined which is considered overall cohort viability (OCVD.m). The previously mentioned indicator considers cohort survival at all stages of ontogenesis. In addition, the expressed in percentage indicator of oppression (IO OCVD.m) in relevance to the control OCVD. m affects the level of air pollution of urban landscapes by emissions of various enterprises. The relevance between these indicators is determined by a four-level scale elaborated specifically for the purpose. The method has been tested based on technogenic landscapes of Chernivtsi, Ukraine. The sensitivity of the suggested indicator for a wide range of pollutants has been proved and its ability to respond to different levels of greening of similar enterprises has been shown.

Eutrophication of the urban water bodies is one the biggest challenge causing severe ecological and economic loss. Urban ponds are more prone to eutrophication due to their small size and polluted catchment areas. Biomonitoring using phytoplankton provides cost-effective estimation of the level of eutrophication. Ten urban ponds in different areas of the Mumbai city were chosen to investigate the phytoplankton community structure, and level of eutrophication. We assessed the 3 algal indices viz. Shannon-Wiener indices, Palmer and Nygard's (Myxophycean and diatom) indices. Linear relationship of these indices was tested against Carlson trophic state indices in order to assess the effectiveness of these indices to measure the degree of eutrophication in urban lakes. All ten lakes were found to be eutrophic, of which two were very low eutrophic (TSI – 53.74-53.95), four were low-mid eutrophic (TSI – 55.18 – 57.5), and four lakes were mid eutrophic (TSI 61.4 – 62.2). Shannon-Wiener indices (r= -0.73) and Myxophycean indices (r= 0.77) showed strong correlation with TSI whereas Diatom indices (r= -0.12) and Palmer’s Algal Pollution Indices (r= - 0.47) showed weak correlation with TSI. Thus study found that Shannon-Wiener indices and Myxophycean indices are reliable and cost effective means to assess the eutrophication of urban ponds in Mumbai.

Marine coastal contamination caused by human activity is a major issue worldwide. The implementation of effective pollution monitoring programs, especially in coastal areas, is important and urgent. The use of biological, physiological, or biochemical measurements to monitor the impacts of pollution has garnered increasing interest, particularly for the development of new non-invasive tools to assess water pollution. Fish skin mucus is in direct contact with the marine environment, making it a favourable microenvironment for the formation of biofilm bacterial communities. In this study, we developed a non-invasive technique, sampling fish skin mucus to determine and analyse bacterial community composition using next-generation sequencing. We hypothesised that bacterial communities associated with the skin mucus of a common harbour benthic blennioid triplefin fish, Forsterygion capito, would reflect conditions of different marine environments. We detected clear differences in bacterial community alpha-diversity between contaminated and reference sites. Beta-diversity analysis also revealed differences in the bacterial community structure of the skin mucus of fish inhabiting different geographical areas. The relative abundance of different bacterial orders varied among sites, as determined by linear discriminant analysis (LDA) and effect size (LEfSe) analyses. The observed variation in bacterial community compositions correlated more strongly with variation in hydrocarbons than to various metal concentrations. Using advanced DNA sequencing technologies, we have developed a novel non-invasive, low-cost and effective tool to monitor the impacts of pollution through analysis of the bacterial communities associated with fish skin mucus.

The occurrence of legacy and novel per- and polyfluoroalkyl substances (PFASs) in multiple matrices from a farmland environment was investigated in the Beijing-Tianjin-Hebei core area of northern China. PFASs were ubiquitously detected in farmland soils, and the detection frequency of 6:2 chlorinated polyfluoroalkyl ether sulfonic acid (6:2 Cl-PFESA) was higher than that of perfluorooctane sulfonic acid (98% vs. 83%). Long-chain PFASs, including 6:2 Cl-PFESA, showed a centered distribution pattern around the metropolis of Tianjin, probably due to the local intensive industrial activity, while trifluoroacetic acid (TFA) showed a decreasing trend from the coast to the inland area. Other than soil, TFA was also found at higher levels than other longer-chain PFASs in dust, maize (Zea mays), poplar (Populus alba) leaf and locust (Locusta migratoria manilens) samples. Both poplar leaves and locusts can be used as promising biomonitoring targets for PFASs in farmland environments, and their accumulation potential corresponds with protein and lipid contents. Apart from being exposed to PFASs via food intake, locusts were likely exposed via uptake from soil and precipitated dust in farmland environments. The biomonitoring of locusts may be more relevant to insectivores, which is important to conducting a comprehensive ecological risk assessment of farmland environments.

The boreoatlantic gonate squid (Gonatus fabricii) represents important prey for top predators—such as marine mammals, seabirds and fish—and is also an efficient predator of crustaceans and fish. Gonatus fabricii is the most abundant cephalopod in the northern Atlantic and Arctic Ocean but the trace element accumulation of this ecologically important species is unknown. In this study, trace element concentrations (Ag, As, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Se, and Zn) were analysed from the mantle muscle and the digestive gland tissue of juveniles, adult females, and adult males that were captured south of Disko Island off West-Greenland. To assess the feeding habitat and trophic position of this species, stable isotopes of carbon (δ13C) and nitrogen (δ15N) were measured in their muscle tissue. Mercury concentrations were positively correlated with size (mantle length) and trophic position. The Hg/Se ratio was assessed because Se has been suggested to play a protective role against Hg toxicity and showed a molar surplus of Se relative to Hg. Cadmium concentrations in the digestive gland were negatively correlated with size and trophic position (δ15N), which suggested a dietary shift from Cd-rich crustaceans towards Cd-poor fish during ontogeny. This study provides trace element concentration data for G. fabricii from Greenlandic waters, which represents baseline data for a northern cephalopod species. Within West-Greenland waters, G. fabricii appears to be an important vector for the transfer of Cd in the Arctic pelagic food web.

Heavy metal contamination is a serious environmental problem commonly monitored in various organisms. Small wild rodents are ideal biological monitors to show the extent of environmental pollution. The aim of this study was to evaluate the adverse effects of marble and stone quarries on the Levant vole, Microtus guentheri, inhabiting some polluted sites. In this context, Inductively Coupled Plasma Mass Spectrometry (ICP-MS) was used to analyze distribution of thirteen heavy metals (Fe, Al, Zn, Cu, Cr, Mn, Ni, B, Pb, As, Co, Cd, and Hg) in the organs (skins, bones, muscles, livers and kidneys) of the biological specimens, and the comet assay revealed DNA damage in blood lymphocytes for the first time. This study was conducted at close to the marble and stone quarries at Korkuteli, Antalya-Turkey during spring, summer, autumn (2017) and winter (2018) seasons. In spring and summer, genetic damage in blood lymphocytes from all polluted sites (sites 1–5) was significantly higher than that of controls, while in autumn it was higher in samples from three sites (sites 3–5). In terms of heavy metal distribution in organs, we found depositions of Fe, Al, Zn, Ni, Mn, Cr, Co, As and Pb primarily in the skin with its derivatives, Cu and Cd deposits in the kidney, Cu, Cd and B deposits in the liver, and As and Pb depositions in the bones. The study shows that certain organs (especially skin with its derivatives) and blood lymphocytes of Levant vole can be used as ideal indicators of heavy metal pollution. Our results suggest that the Korkuteli area could already be under the threat of heavy metal pollution.

In this study, coral soft tissue, skeleton and zooxanthellae, as well as their ambient sediment and seawater were analyzed for polycyclic aromatic hydrocarbons (PAHs) with a special focus on perylene. Samples were collected from two different environments: the Kharg Island, which is affected by numerous anthropogenic stressors and Larak Island, which is mainly used for recreational and fishing activities and is characterized by dense vegetation. The heaviest loadings of PAHs were observed on Kharg Island, yet higher concentrations of perylene were detected on Larak Island and it was identified as the prevailing compound in this area. Pyrogenic perylene sources were prevailing on Kharg Island, whereas the perylene on Larak Island was determined to be of natural origin. After analyzing the biological samples, higher perylene concentrations were observed in zooxanthellae than in tissue and skeleton. The lowest and the highest perylene loadings were found in the tissue and skeleton of Platygyra daedalea and Porites lutea, respectively. This applies to both reefs. We found that perylene distribution in the corals and their ambient environment follows an irregular pattern, demonstrating remarkable effects from the local inputs. The lipid content in the coral tissue and the location of the coral colony were deduced to be the main factors affecting perylene distribution in corals. On Larak Island, a significant correlation between perylene loadings in sediment and corals was observed. On Kharg Island, a strong interaction between the water column and the corals was detected. The symbiotic relationship between the corals and zooxanthellae might play the most significant role in bioconcentration and bioaccumulation of perylene. Due to the insolubility of PAHs, they could be transferred through a food chain to zooxanthellae and eventually deposited in the coral bodies.

Organophosphate esters (OPEs) are high-production volume chemicals. Use of OPEs has largely increased since the phase-out/ban of polybrominated diphenyl ethers (PBDEs). The ubiquitous occurrence of OPEs, in higher concentrations in abiotic matrices than brominated flame retardants (BFRs), is a concern because several of the OPEs have been linked to adverse health effects. In this study, urinary metabolites of OPEs were measured in a subset of a population-based sample of women of child bearing age recruited in Ontario, and associations between serum lipid levels and urinary concentrations of OPE metabolites were evaluated. Urine samples (n = 120) were extracted using automated solid phase extraction and analysed by ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). Diphenyl phosphate (DPHP), bis(2-chloropropyl) phosphate (BCIPP) and bis(1,3-dichloro-2 propyl) phosphate (BDCIPP) were detected with frequencies of 100%, 76% and 75% at median concentrations of 13.8 ng/mL, 0.5 ng/mL and 1.8 ng/mL, respectively. Bis(2-chloroethyl) hydrogen phosphate (BCEP) and di-cresyl phosphate (DCP; mixture of 3 isomers) were detected in 52% and 42% of the samples, respectively. Detected at lower frequencies were 1-hydroxy-2-propyl bis(1-chloro-2-propyl) phosphate (BCIPHIPP, 29%), bis-2(butoxyethyl) phosphate (BBOEP, 11%), and desbutyl-tris-(2-butoxy-ethyl) phosphate (desbutyl TBOEP, 9%). Using multiple regression model, a negative statistically significant correlation was observed between BCEP and cholesterol (p = 0.04), as well as BCEP and total lipid (p = 0.04). Whereas BCIPP was positively and significantly correlated with cholesterol (p = 0.003) and LDL (p = 0.001). Additional work to further explore these relationships and to evaluate more recently identified OPE metabolites is warranted.

Reservoirs are lentic man-made waterbodies resulting from river damming processes. Pollutants coming from adjacent areas can accumulate in the water and sediment of these modified freshwater environments. Fish are often found in reservoirs occupying several trophic niches. Biochemical biomarkers are early warning signals of environmental disturbance to an organism. It is essential to understand how pollutants, abiotic variables and biochemical biomarker responses behave throughout the seasons to implement biomonitoring programs. Loricariichthys anus and Geophagus brasiliensis were collected, and abiotic variables were seasonally measured for one year, at six sampling sites in Passo Real reservoir, in a subtropical region of Southern Brazil. Biochemical biomarkers were analyzed in four tissues of both fish species, as well as metal and pesticide concentrations in the reservoir’s water and sediment. Redundancy analysis (RDA) was carried out to find the temporal relationship between biomarkers and environmental variables. RDA has clearly shown the separation of seasons for both species. Azoxystrobin, simazine and propoxur were the pesticides mostly contributing to the variation, whereas metals had lesser contribution to it. Seasonality appears to be the main factor explaining biomarkers’ variability. PERMANOVA has confirmed the effect of temperature and dissolved oxygen on biomarkers of both fish species. Thus, it is hard to differentiate if the fluctuation in biomarkers’ responses only reflects the normal state of organisms or it is a biological consequence from negative effects of fish exposure to several types of pollution (sewage, pesticides, and fertilizers) entering this aquatic system. In this study, to circumvent the seasonality issue on biomonitoring, the analysis of biomarkers on these fish should not be carried out in organs directly affected by temperature (such as liver and gills), or during reproduction periods (mainly in Spring).

Atmospheric heavy metal contamination is becoming a serious threat to environmental and human health in Chinese megacities. This study evaluated the concentrations of arsenic (As), cadmium (Cd), copper (Cu), lead (Pb) and zinc (Zn) and Pb isotopic compositions in herbarium and native bryophytes collected from Guangzhou from 1932 to 2018. Relatively low mean metal concentrations were measured for bryophytes collected in the 1930s. The highest mean concentrations of Cd (0.72 ± 0.32 mg/kg), Cu (28.1 ± 9.8 mg/kg), Pb (125.9 ± 62.4 mg/kg) and Zn (273 ± 130 mg/kg) were found in the bryophytes from 1979 to 2000, following the commencement of the Reform and Opening-Up Program in 1978. The mean Pb concentrations (74.7 ± 6.3 mg/kg) decreased sharply from 2001 onwards, following the cessation of leaded petrol across the Chinese mainland in 2000. However, these values are still higher than those in 1950–1978, corresponding to a significant increase in atmospheric Pb emissions from coal combustion, nonferrous metal smelting and motor vehicle petrol consumption in China in the 2000s. The lead isotopic ratios of bryophyte archives (²⁰⁶Pb/²⁰⁷Pb 1.141–1.229, ²⁰⁸Pb/²⁰⁷Pb 2.376–2.482) indicate that lithogenic input and anthropogenic input arising from leaded petrol and industrial emissions have been the main sources of atmospheric heavy metal deposition in the city of Guangzhou over the past 85 years. Herbarium bryophyte can be utilised to reconstruct temporal and spatial shifts in atmospheric heavy metal deposition to better understand and manage the current air quality in Chinese megacities.