The goal of this study was to evaluate the adverse effects of wastewater effluents on freshwater crucian carp, Carassius auratus, inhabiting Sincheon stream using the integrated biomarker response (IBR) at the genotoxic (micronucleus [MN] test), oxidative stress (activity of catalase [CAT] and glutathione S-transferase [GST], and level of lipid peroxidation [LPO]), histopathological (degree of tissue changes [DTC]), and physiological (condition factor [CF] and liver somatic index [LSI]) levels. The CF and LSI were significantly (p < 0.05) enhanced in fish from downstream sites (DS1 and DS2) as compared to that of upstream (US) fish samples. Moreover, a significant increase in morphometric indices (DTC) was observed in C. auratus collected from downstream sites (p < 0.05) and histopathological responses showed the degree of pathogenicity in the order of liver > kidney > gills. The activities of CAT, GST, and LPO in fish from the DS1 and DS2 sites were notably increased in gills, liver, and kidney compared to that of fish from the US site. Additionally, the MN test level in C. auratus from the DS1 and DS2 were significantly increased (p < 0.05) when compared with that of the US site. Considering the higher bioaccumulation of Cd, Co, Cr, Mn, Ni, and Pb in gills, liver, and kidney of C. auratus collected from downstream sites compared to that of the upstream site (p < 0.05), the observed toxicity was likely attributable to metal accumulation. The multi-level IBR index was higher at the DS1 site (15.08) than at the DS2 (1.02) and the reference US (0.00) sites. Therefore, these findings demonstrated that wastewater effluent discharge induces significant DNA damage, oxidative stress, and tissue injuries in C. auratus and suggested that the multi-level IBR approach should be used to quantify these effects on fish in streams and rivers.

The objective of this study was to assess potential toxic effects of simulated urban runoff on Carassius auratus using oxidative stress biomarkers. The activity of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and the content of malondialdehyde (MDA) in the liver of C. auratus were analyzed after a 7-, 14- and 21-day exposure to simulated urban runoff containing galaxolide (HHCB) and cadmium (Cd). The results showed that the activity of antioxidant enzymes and the content of MDA increased significantly exposed to the simulated urban runoff containing HHCB alone or mixture of HHCB and Cd. The activity of the investigated enzymes and the content of MDA then returned to the blank level over a longer period of exposure. The oxidative stress could be obviously caused in the liver of C. auratus under the experimental conditions. This could provide useful information for toxic risk assessment of urban runoff.

Freshwater environments are more sensitive to anthropogenic influences and usually contain higher concentrations of pollutants than marine environments. Microplastic pollution causes additional stress on freshwater animals; yet, studies evaluating the microplastic occurrence in freshwater biota are still limited. In this study, microplastic occurrence in the gastrointestinal tracts (GIT) and gill of commercial fish species (Prussian carp Carassius gibelio (Bloch, 1782); Abu mullet Planiliza abu (Heckel, 1843); Common carp Cyprinus carpio Linnaeus, 1758; European ell Anguilla Anguilla (Linnaeus, 1758); North African catfish Clarias gariepinus (Burchell, 1822); Goldfish Carassius auratus (Linnaeus, 1758) were reported from Orontes River. MPs abundance in the GIT and gill of six species were found as 5.1 ± 2 MPs fish⁻¹ and 4.4 ± 2 MPs fish⁻¹ with an occurrence of 95% and 74%, respectively. The majority of extracted microplastics were fiber, black and less than 1000 μm in size. FTIR analysis determined the main polymer types as polyester (50%), high-density polyethylene (HDPE) (10%), polypropylene (PP) (8%) and polyethylene terephthalate (PET) (5%). High MPs abundance and frequency of occurence indicate the exposure of microplastic pollution in freshwater biota which could threat the health of both individuals and consumers. Results obtained in this study will increase the acknowledgement of MPs pollution in the Orontes River. Also, this study will provide data to the administrators to set up necessary legislations in freshwater ecosystems.

Bisphenol A (BPA) is a contaminant whose presence in aquatic environments is increasing. In fish embryos and larvae, it severely affects cardiac development; however, its influence on the heart function of adult fish has been scarcely analyzed. This study investigated the effects of the in vivo exposure to BPA on heart physiology, morphology, and oxidative balance in the goldfish Carassius auratus. Adult fish were exposed for 4 and 10 days to two BPA concentrations (10 μM and 25 μM). Ex vivo working heart preparations showed that high concentrations of BPA negatively affected cardiac hemodynamics, as revealed by an impaired Frank-Starling response. This was paralleled by increased cardio-somatic indices and by myocardial structural changes. An altered oxidative status and a modulation of stress (HSPs) and pro-apoptotic (Bax and Cytochrome C) proteins expression were also observed in the heart of animals exposed to BPA, with detrimental effects at the highest concentration and the longest exposure time. Results suggest that, in the adult goldfish, BPA may induce stressful conditions to the heart with time- and concentration-dependent deleterious morpho-functional alterations.

Freshwater ecosystems are facing increasing contamination by major ions. The Multi-Ion Toxicity (MIT) model, a new tool for risk assessment and regulation, predicts major ion toxicity to aquatic organisms by relating it to a critical disturbance of the trans-epithelial potential (TEP) across the gills, as predicted by electrochemical theory. The model is based on unproven assumptions. We tested some of these by directly measuring the acute TEP responses to a geometric series of 10 different single salts (NaCl, Na₂SO₄, KCl, K₂SO₄, CaCl₂, CaSO₄, MgCl₂, MgSO₄, NaHCO₃, KHCO₃) in the euryhaline rainbow trout (Oncorhynchus mykiss) and the stenohaline goldfish (Carassius auratus) acclimated to very soft, ion-poor water (hardness 10 mg CaCO₃/L). Results were compared to 24-h and 96-h LC50 data from the literature, mainly from fathead minnow (Pimephales promelas). All salts caused concentration-dependent increases in TEP to less negative/more positive values, in patterns well-described by the Michaelis-Menten equation, or a modified version incorporating substrate inhibition. The ΔTEP above baseline became close to a maximum at the 96-h LC50, except for the HCO₃⁻ salts. Furthermore, the range of ΔTEP values at the LC50 within one species was much more consistent (1.6- to 2.1-fold variation) than the molar concentrations of the different salts at the LC50 (19- to 25-fold variation). ΔTEP responses were related to cation rather than anion concentrations. Overall patterns were qualitatively similar between trout and goldfish, with some quantitative differences, and also in general accord with recently published data on three other species in harder water where ΔTEP responses were much smaller. Blood plasma Na⁺ and K⁺ concentrations were minimally affected by the exposures. The results are in accord with most but not all of the assumptions of the MIT model and support its further development as a predictive tool.

Endocrine disrupting chemicals (EDCs) found in wastewater treatment plant (WWTP) effluents have been shown to cause adverse effects, but the uptake of EDCs from effluents (measured in fish muscle) are not known. In this study, the biological effects and bioaccumulation of steroidal and phenolic EDCs were assessed in high-back crucian carp (Carassius auratus) exposed to WWTP effluents for 141 days. Compared with fish controls caged in Dianchi Lake, a significant reduction in gonadosomatic index (GSI) and increase in hepatosomatic index (HSI) and plasma vitellogenin (VTG) levels were observed in effluent-exposed fish. The concentrations of steroids and phenols in effluent-exposed fish showed time-dependent increase during the exposure. In addition, bioconcentration factors (BCFs) for steroids and phenols were between 17 and 59 on day 141. The results confirm that steroids and phenols bioconcentrate in fish muscle and this accumulation may account for the biological effects associated with exposures to WWTP effluents.

The potentially toxic elements (PTEs) concentrations in water and sediments were measured in the Hoor Al-Azim wetland to evaluate the spatial distribution, pollution rate, fate, partitioning, and ecological risk and also to recognize the PTEs sources in sediments using MLR-APCs (multiple linear regression-absolute principal component scores) receptor model. The human health risk was investigated based on the seven fish species consumed in the study area. Based on the results, water and sediment contamination was observed at some stations in the southern part of the wetland where agricultural water drains. Also, the sediments of oil well drilling disposal site was polluted by PTEs. Based on the MLR-APCs model, 80.8% of Mo and 81.5% of Se originated from agricultural source. Total target hazard quotients (TTHQ) values suggested that the children could experience adverse health effects due to consumption of Coptodon zillii, Aspius vorax, Carassius auratus and Carasobarbus luteus.

Vibrio parahaemolyticus can cause severe gastroenteritis, septicaemia and even death in humans. Continuous monitoring of V. parahaemolyticus contamination in aquatic products is imperative for ensuring food safety. In this study, we isolated and characterized 561 V. parahaemolyticus strains recovered from 23 species of commonly consumed shellfish, crustaceans, and fish collected in July and August of 2017 in Shanghai, China. The bacterium was not isolated from two fish species Carassius auratus and Parabramis pekinensis. The results revealed a very low occurrence of pathogenic V. parahaemolyticus carrying the toxin genes trh (0.2%) and tdh (0.0%). However, high percentages of resistance to the antimicrobial agents ampicillin (93.0%), rifampin (82.9%), streptomycin (75.4%) and kanamycin (50.1%) were found. A high incidence of tolerance to the heavy metals Hg²⁺ (74.7%) and Zn²⁺ (56.2%) was also observed in the isolates. ERIC-PCR-based fingerprinting of MDR isolates (77.5%) revealed 428 ERIC-genotypes, demonstrating remarkable genetic variation among the isolates. The results of this study support the urgent need for food safety risk assessment of aquatic products.

This study used the integrated biomarker response (IBR) index approach to assess the ecological risks of heavy metals in different regions of Dianchi Lake, combined with active monitoring and passive monitoring. The contents of five heavy metals (Cu, As, Cd, Hg, and Pb) and six biomarkers (acetylcholinesterase, sodium-potassium ATPase, metallothionein, superoxide dismutase, glutathione peroxidase, and malondialdehyde) in the muscles of crucians (Carassius auratus) were measured to calculate the IBR value. The results indicate that the contents of heavy metal in the fish under active monitoring and passive monitoring were rather low and did not exceed the National Food Safety Standards of China. The IBR value of day 14 of active monitoring correlated with the heavy metal Cd content in the fish, suggesting a potential risk of Cd pollution in the aquatic environment of Dianchi Lake. The IBR values obtained for different regions of the lake on day 14 can be arranged in the following order: West S3 (9.24) > East S1 (3.97) > South S2 (2.39) > North S4 (0.36). These results suggest a potential risk of heavy metal contamination in the western part of Dianchi Lake.

The effluents from bleached Kraft pulp mill (BKME) and paper industry are toxic to different aquatic organisms being an important source of contamination to aquatic environments due to the presence of several chemicals produced during the production of Kraft pulp. The main objective of the present study was to evaluate the exposure effects of a secondary-treated BKME in two different species of fish: Carassius auratus and Dicentrarchus labrax. Both species were exposed to different concentrations of secondary-treated effluent (1%, 10%, 25%, 50%, 100%) in semi-static tests under controlled laboratory conditions. At the end of the experimental period (21 days), samples of livers were collected for CYP1A determination and histopathological evaluation. The results show significant changes (p < 0.05) of CYP1A induction in carp exposed to 50% and in sea bass exposed to 25% of the effluent. Histopathological alterations were also observed according to the different concentrations of the tested effluent suggesting that tested BKME cause damage to exposed organisms.