Toxic metalloids including arsenic (As) can neither be eliminated nor destroyed from environment; however, they can be converted from toxic to less/non-toxic forms. The form of As species and their concentration determines its toxicity in plants. Therefore, the microbe mediated biotransformation of As is crucial for its plant uptake and toxicity. In the present study the role of As tolerant Trichoderma in modulating As toxicity in chickpea plants was explored. Chickpea plants grown in arsenate spiked soil under green house conditions were inoculated with two plant growth promoting Trichoderma strains, M-35 (As tolerant) and PPLF-28 (As sensitive). Total As concentration in chickpea tissue was comparable in both the Trichoderma treatments, however, differences in levels of organic and inorganic As (iAs) species were observed. The shift in iAs to organic As species ratio in tolerant Trichoderma treatment correlated with enhanced plant growth and nutrient content. Arsenic stress amelioration in tolerant Trichoderma treatment was also evident through rhizospheric microbial community and anatomical studies of the stem morphology. Down regulation of abiotic stress responsive genes (MIPS, PGIP, CGG) in tolerant Trichoderma + As treatment as compared to As alone and sensitive Trichoderma + As treatment also revealed that tolerant strain enhanced the plant's potential to cope with As stress as compared to sensitive one. Considering the bioremediation and plant growth promotion potential, the tolerant Trichoderma may appear promising for its utilization in As affected fields for enhancing agricultural productivity.

Polycyclic aromatic hydrocarbons (PAHs) are recalcitrant organic pollutants generated from agricultural, industrial, and municipal sources, and their strong carcinogenic and teratogenic properties pose a harmful threat to human beings. The present study deals with the bioremediation of phenanthrene by a ligninolytic fungus, Coriolopsis byrsina (Mont.) Ryvarden strain APC5 (GenBank; KY418163.1), isolated from the fruiting body of decayed wood surface. During the experiment, Coriolopsis byrsina strain APC5 was found as a promising organism for the degradation and detoxification of phenanthrene (PHE) in in vitro and in vivo conditions. Further, HPLC analysis showed that the C. byrsina strain degraded 99.90% of 20 mg/L PHE in in vitro condition, whereas 77.48% degradation of 50 mg/L PHE was reported in in vivo condition. The maximum degradation of PHE was noted 25 °C temperature under shaking flask conditions at pH 6.0. Further, GC-MS analysis of fungal treated samples showed detection of 9,10-Dihydroxy phenanthrene, 2,2-Diphenic acid, phthalic acid, 4-heptyloxy phenol, benzene octyl, and acetic acid anhydride as the metabolic products of degraded PHE. Furthermore, the phytotoxicity evaluation of degraded PHE was observed through the seed germination method using Vigna radiata and Cicer arietinum seeds. The phytotoxicity results showed that the seed germination index and vegetative growth parameters of tested plants were increased in the degraded PHE soil. As results, C. byrsina strain APC5 was found to be a potential and promising organism to degrade and detoxify PHE without showing any adverse effect of their metabolites.

Mine tailings can contaminate large areas of neighboring agricultural lands due to the dispersion of heavy metals, which may lead to reduction in soil fertility and toxicity in crops. The use of Fabaceae species as green manure to amend the soil and enhance the removal of heavy metals is a promising research approach. As part of a phytoremediation project for abandoned mining sites combining woody species and agricultural crops, this study aims to identify the most suitable species to be used. Thus, four Fabaceae species (Vicia faba, Cicer arietinum, Lens culinaris, and Medicago arborea) were subjected to muti-metal-contaminated soil containing high concentrations of Pb, Zn, and Cd and to control soil for 15 days. Then, the emergence rate, growth parameters, lipid peroxidation, proline and hydrogen peroxide (H₂O₂) concentrations, antioxidant enzyme activities (catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (GPX)), and heavy metals accumulation were evaluated. Results showed that V. faba was the most tolerant. A relative sensitivity at the germination stage was recorded for all species with the exception of V. faba. Metallic stress had no significant effect on thiobarbituric acid reactive susbtances (TBARS) and electrolyte leakage rates for both C. arietinum and V. faba. The latter also showed the highest proline concentration and relatively low antioxidant enzyme activities. All species showed high Pb, Zn, and Cd root contents. V. faba had the lowest translocation factors of Pb and Zn and the lowest bioaccumulation factors of Zn and Cd, which underline its phytostabilizing potential and support its use as green manure for heavy metals contaminated soils amendement and rehabilitation.

Crustacean shell waste disposal is considered as biggest problem in seafood processing centers. Incineration and landfilling are the commonest ways of disposal of the waste which causes environmental pollution. Microbial bio-conversion is one of the promising approaches to minimize the wastes by utilizing the same for deriving different value added metabolites. In this perspective, chitinase- and protease-producing bacterial strains were isolated from shrimp culture pond, and the potent isolate was subsequently identified as Alcaligenes faecalis SK10. Fermentative optimization of the production of chitinase (85.42 U/ml), protease (58.57 U/ml), and their catalytic products, viz., N-acetylamino sugar (84 μg/ml) and free amino acids (112 μg/ml), were carried out by utilizing shrimp and crab shell powder as principal substrate. The fermented hydrolysate (FH) was subsequently applied to evaluate its potential to be a candidate fertilizer for the growth of leguminous plant Pisum sativum and Cicer arietinum, and the results were compared with chitin, chitosan, and commercial biofertilizer amended group. The results revealed that FH have paramount potential to improve plants morpho-physiological parameters like stem and root length, chlorophyll, cellular RNA, protein content, and soil physico-chemical parameters like total nitrogen, magnesium, calcium, phosphorus, and potassium significantly (p < 0.05). Moreover, the application of FH also selectively encouraged the growth of free-living nitrogen-fixing bacteria, Rhizobium, phosphate-solubilizing bacteria in the soil by 4.82- and 5.27-, 5.57- and 4.71, and 7.64- and 6.92-fold, respectively, in the rhizosphere of P. sativum and C. arietinum, which collectively is a good sign for an ideal biofertilizer. Co-supplementation of FH with commercial PGPR-biofertilizer significantly influenced the morpho-physiological attributes of plant and physico-chemical and microbial attributes of soil. The study validated proficient and sustainable utilization of fermented hydrolysate of waste crustacean shell as biofertilizer.

The present study was done to elucidate the effects of vanadium (V) on photosynthetic pigments, membrane damage, antioxidant enzymes, protein, and deoxyribonucleic acid (DNA) integrity in the following chickpea genotypes: C-44 (tolerant) and Balkasar (sensitive). Changes in these parameters were strikingly dependent on levels of V, at 60 and 120 mg V L⁻¹ induced DNA damage in Balkasar only, while photosynthetic pigments and protein were decreased from 15 to 120 mg V L⁻¹ and membrane was also damaged. It was shown that photosynthetic pigments and protein production declined from 15 to 120 mg V L⁻¹ and the membrane was also damaged, while DNA damage was not observed at any level of V stress in C-44. Moreover, the antioxidant enzyme activities such as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) were increased in both genotypes of chickpea against V stress; however, more activities were observed in C-44 than Balkasar. The results suggest that DNA damage in sensitive genotypes can be triggered due to exposure of higher vanadium.

Purpose Spillage of petroleum hydrocarbons causes significant environmental pollution. Bioremediation is an effective process to remediate petroleum oil contaminant from the ecosystem. The aim of the present study was to reclaim a petroleum oil-contaminated soil which was unsuitable for the cultivation of crop plants by using petroleum oil hydrocarbon-degrading microbial consortium. Materials and methods Bacterial consortium consisting of Bacillus subtilis DM-04 and Pseudomonas aeruginosa M and NM strains were seeded to 20% (v/w) petroleum oil-contaminated soil, and bioremediation experiment was carried out for 180 days under laboratory condition. The kinetics of hydrocarbon degradation was analyzed using biochemical and gas chromatographic (GC) techniques. The ecotoxicity of the elutriates obtained from petroleum oil-contaminated soil before and post-treatment with microbial consortium was tested on germination and growth of Bengal gram (Cicer aretinum) and green gram (Phaseolus mungo) seeds. Results Bacterial consortium showed a significant reduction in total petroleum hydrocarbon level in contaminated soil (76% degradation) as compared to the control soil (3.6% degradation) 180 days post-inoculation. The GC analysis confirmed that bacterial consortium was more effective in degrading the alkane fraction compared to aromatic fraction of crude petroleum oil hydrocarbons in soil. The nitrogen, sulfur, and oxygen compounds fraction was least degraded. The reclaimed soil supported the germination and growth of crop plants (C. aretinum and P. mungo). In contrast, seeds could not be germinated in petroleum oil-contaminated soil. Conclusions The present study reinforces the application of bacterial consortium rather than individual bacterium for the effective bioremediation and reclamation of soil contaminated with petroleum oil.