Constructed wetland effectiveness is often assessed by measuring reductions of contaminant concentrations in influent versus departing effluent, but this can be complicated by fluctuations in contaminant content/chemistry and hydrology. We assessed effectiveness of a constructed wetland at protecting downstream biota from accumulating elevated metal concentrations—particularly copper and zinc in effluents from a nuclear materials processing facility. Contaminants distributed throughout a constructed wetland system and two reference wetlands were assessed using six dragonfly nymph genera (Anax, Erythemis, Libellula, Pachydiplax, Tramea, and Plathemis) as biomonitors. Additionally, the crayfish, Cambarus latimanus, were analyzed from the receiving and two reference streams. Concentrations of Cu, Zn, Pb, Mn, Cr, Cd, and Al were evaluated in 597 dragonfly nymph and 149 crayfish whole-body composite samples. Dragonfly genera varied substantially in metal accumulation and the ability to identify elevated metal levels throughout components of the constructed wetland. Genera more closely associated with bottom sediments tended to accumulate higher levels of metals with Libellula, Pachydiplax, and Erythemis often accumulating highest concentrations and differing most among sites. This, combined with their abundance and broad distributions make the latter two species suitable candidates as biomonitors for constructed wetlands. As expected, dragonfly nymphs accumulated higher metal concentrations in the constructed wetland than reference sites. However, dragonfly nymphs often accumulated as high of metal concentrations downstream as upstream of the water treatment cells. Moreover, crayfish from the receiving stream near the constructed wetland accumulated substantially higher Cu concentrations than from downstream locations or reference streams. Despite reducing metal concentrations at base flow and maintaining regulatory compliance, metal fluxes from the wetland were sufficient to increase accumulation in downstream biota. Future work should evaluate the causes of downstream accumulation as the next step necessary to develop plans to improve the metal sequestering efficiency of the wetland under variable flow regimes.

Penconazole is one of the most widely used fungicides all over the world, and since it spreads to large environments, its toxic effects on non-target organisms are of great concern. The toxic effects of penconazole on crayfish (Astacus leptodactylus), which is a bioindicator in freshwater ecosystems and consumed economically, are not known. Therefore, in this study, the purpose was to contribute to the literature on the potential harmful effects of penconazole on a non-target species, Astacus leptodactylus. For this aim, the acute toxicity (96 h) of penconazole was examined. The 96-h LC₅₀ value of penconazole was detected as 18.7 mg L⁻¹. Four concentrations of penconazole (18.7 mg L⁻¹, 9.35 mg L⁻¹, 4.68 mg L⁻¹, 2.34 mg L⁻¹) were applied to crayfish for 96 h. The results showed that penconazole had destructive effects on esterase mechanisms by inhibiting acetylcholinesterase (AChE) and carboxylesterase (CaE) activities. Significant increases were observed in all antioxidant parameters (superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferase (GST), reduced glutathione (GSH), malondialdehyde (MDA)) in all doses except the lowest concentration (2.34 mg L⁻¹). All adenosine triphosphatase (ATPase) activities (Na⁺/K⁺-ATPase, Mg²⁺-ATPase, Ca²⁺-ATPase, total ATPase) had significant dose-related inhibition in both gill and muscle tissues. In summary, our findings show that acute penconazole administration to crayfish causes significant toxic effects on esterase, antioxidative parameters, and metabolic enzymes.

In this study, crayfish shell was pyrolyzed at 600 °C to obtain an unmodified biochar (CS600). MgCl₂ was used as a modifier to pretreat crayfish shell to produce a modified biochar (CS600-MgCl₂) under the same pyrolysis conditions. The two biochars were characterized for physicochemical properties and evaluated for lead (Pb²⁺) sorption ability to determine the modification mechanism. Mono-element batch adsorption experiments were conducted to compare the sorption performances of CS600 and CS600-MgCl₂ to Pb²⁺ in aqueous solutions. All the experiments were carried out at pH of 7. According to the Freundlich–Langmuir model, CS600-MgCl₂ had a higher adsorption capacity (152.3 mg/g) than CS600 (134.3 mg/g). FTIR, SEM, XRD, BET, and ICP analyses were applied to inform the interpretation of the mechanism. CS600 was calcium-rich and mainly removed Pb²⁺ through the ion exchange mechanism by replacing Ca²⁺ in the biochar. The increased Pb²⁺ adsorption capacity of CS600-MgCl₂ was mainly due to the enlarged specific surface area and the formation of Mg₃(OH)₅Cl·4H₂O on the modified biochar. Findings of this study suggest that both CS600 and CS600-MgCl₂ can be used to remove heavy metal ions from wastewater and MgCl₂ can improve the sorption performance of biochar.