Recently, there has been a growing concern that climate change may rapidly and extensively alter global ecosystems with unknown consequences for terrestrial and aquatic life. While considerable emphasis has been placed on terrestrial ecology consequences, aquatic environments have received relatively little attention. Limited knowledge is available on the biological effects of increments of seawater temperature and pH decrements on key ecological species, i.e., primary producers and/or organisms representative of the basis of the trophic web. In the present study, we addressed the biological effects of global warming and ocean acidification on two model organisms, the microbenthic marine ciliate Euplotes crassus and the green alga Dunaliella tertiocleta using a suite of high level ecological endpoint tests and sub-lethal stress measures. Organisms were exposed to combinations of pH and temperature (TR1: 7.9[pH], 25.5 °C and TR2: 7.8[pH], 27,0 °C) simulating two possible environmental scenarios predicted to occur in the habitats of the selected species before the end of this century. The outcomes of the present study showed that the tested scenarios did not induce a significant increment of mortality on protozoa. Under the most severe exposure conditions, sub-lethal stress indices show that pH homeostatic mechanisms have energetic costs that divert energy from essential cellular processes and functions. The marine protozoan exhibited significant impairment of the lysosomal compartment and early signs of oxidative stress under these conditions. Similarly, significant impairment of photosynthetic efficiency and an increment in lipid peroxidation were observed in the autotroph model organism held under the most extreme exposure condition tested.

Potassium cyanide (KCN), a highly water soluble and bioaccumulative cyanide salt, is examined to determine the toxic effects by using two green algae (Dunaliella viridis, Nannochloropsis oculata) and genetically different two sea urchin (Paracentrotus lividus, Arbacia lixula) species. To determine the toxic effects on the early developmental stages of sea urchin embryos, 72-hour embryotoxicity studies were conducted. Potassium cyanide toxicity at cellular level was also investigated and 6-hour embryos of both sea urchin species were used to determine genotoxic effects of KCN. Since plutei naturally feed on microalgae, two species of plankton were used to reveal phytotoxic effects of KCN. KCN was found to be embryo- geno- and phytotoxic. EC₅₀'s for P. lividus and A. lixula were found 7.96 and 6.52 μM. IC₅₀'s for N. oculata for 48 h and 72 h were found 23.66 and 80.45 μM. IC₅₀'s for D. viridis for 48 h and 72 h were found 14.31 and 23.36 μM.

Phytoplankton structure and patterns are key components to forecast the net result of the gain and loss process that outline the resilience of the lagoon ecosystem. In order to understand the phytoplankton community structure and its relationship with the environmental variables in the shallow hypersaline Pulicat lagoon, east coast of India, observations were carried out during August 2018–January 2019 covering the three seasons: premonsoon (PrM), monsoon (M), and postmonsoon (PoM). The salinity of the lagoon varied with a minimum of 12.1 for the M and a maximum of 81.65 during the PoM. The clustering analysis performed on the phytoplankton abundance data separated the lagoon into three sectors: north sector (NS), central sector (CS), and south sector (SS). A total of 59 taxa/morphotypes from four taxonomic classes (Bacillariophyceae, Chlorophyceae, Cyanophyceae, and Dinophyceae) were recorded during the study period. The class Bacillariophyceae was dominant in the lagoon both spatially and temporally by 44.06% with Chaetoceros borealis as dominant species. Presence of characteristic species like Dunaliella sp. was observed in the higher salinity, whereas Pediastrum duplex and Scenedesmus sp. were dominant in the freshwater influx areas. The individual-based functional approach allowed grouping these taxa into 11 functional entities based on the derived 4 functional trait values (cell size, trophic regime, mobility, and coloniality). Formation of algal blooms of Protoperidinium sp. (3.3×10⁵ind L⁻¹) and Odentella sp. (2.8×10⁵ind L⁻¹) was observed in the SS during PoM as a result of reduced water exchange in the lagoon. During the same period, toxin-producing strains like Anabaena sp. and Nostoc sp. of Cyanophyceae were also recorded. Correlating the three sectors of the lake (NS, CS, and SS), it is observed that the physical, chemical, and biological properties of the lake varied continuously depending on the season and freshwater availability. Seasonal nutrient stoichiometry played a significant role in regulating the community structure and distribution pattern of phytoplankton communities of the lagoon.