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Effects of four food dyes on development of three model species, Cucumis sativus, Artemia salina and Danio rerio: Assessment of potential risk for the environment
2019
Motta, Chiara Maria | Simoniello, Palma | Arena, Carmen | Capriello, Teresa | Panzuto, Raffaele | Vitale, Ermenegilda | Agnisola, Claudio | Tizzano, Monica | Avallone, Bice | Ferrandino, Ida
Food dyes, or color additives, are chemicals added to industrial food products and in domestic cooking to improve the perceived flavor and attractiveness. Of natural and synthetic origin, their safety has been long discussed, and concern for human safety is now clearly manifested by warnings added on products labels. Limited attention, however, has been dedicated to the effects of these compounds on aquatic flora and fauna. For this reason, the toxicity of four different commercially available food dyes (cochineal red E120, Ponceau red E124, tartrazine yellow E102 and blue Patent E131) was assessed on three different model organisms, namely Cucumis sativus, Artemia salina and Danio rerio that occupy diverse positions in the trophic pyramid. The evidence collected indicates that food dyes may target several organs and functions, depending on the species. C. sativus rate of germination was increased by E102, while root/shoot ratio was ∼20% reduced by E102, E120 and E124, seed total chlorophylls and carotenoids were 15–20% increased by E120 and 131, and total antioxidant activity was ∼25% reduced by all dyes. Mortality and low mobility of A. salina nauplii were increased by up to 50% in presence of E124, E102 and E131, while the nauplii phototactic response was significantly altered by E102, E120 and E124. Two to four-fold increases in the hatching percentages at 48 h were induced by E124, E102 and E131 on D. rerio, associated with the occurrence of 20% of embryos showing developmental defects. These results demonstrated that the food dyes examined are far from being safe for the aquatic organisms as well as land organisms exposed during watering with contaminated water. The overall information obtained gives a realistic snapshot of the potential pollution risk exerted by food dyes and of the different organism' ability to overcome the stress induced by contamination.
اظهر المزيد [+] اقل [-]Aerosol deposition doses in the human respiratory tree of electronic cigarette smokers
2015
Manigrasso, Maurizio | Buonanno, Giorgio | Fuoco, Fernanda Carmen | Stabile, Luca | Avino, Pasquale
Aerosols from eight e-cigarettes at different nicotine levels and flavoring were characterized as particle number size distributions in the range 5.6–560 nm by FMPS and CPC. Results were used to provide dosimetry estimates applying the MMPD model.Particle number concentrations varied between 3.26 × 109 and 4.09 × 109 part cm−3 for e-liquids without nicotine and between 5.08 × 109 and 5.29 × 109 part cm−3 for e-liquids with nicotine. No flavor effects were detected on particle concentration data. Particle size distributions were unimodal with modes between 107–165 nm and 165–255 nm, for number and volume metrics, respectively.Averagely, 6.25 × 1010 particles were deposited in respiratory tree after a single puff. Highest deposition densities and mean layer thickness of e-cigarette liquid on the lung epithelium were estimated at lobar bronchi.Our study shows that e-cigarette aerosol is source of high particle dose in respiratory system, from 23% to 35% of the daily dose of a no-smoking individual.
اظهر المزيد [+] اقل [-]Nanoselenium foliar application enhances biosynthesis of tea leaves in metabolic cycles and associated responsive pathways
2021
Li, Dong | Zhou, Chunran | Zou, Nan | Wu, Yangliu | Zhang, Jingbang | An, Quanshun | Li, Jia-Qi | Pan, Canping
An emerging stress of pesticides in plant and soil is closely watched as it affects crop antioxidant systems, nutritional quality, and flavor. Although selenium (Se) can enhance the resistance of plants, the protective mechanism of nanoselenium is still not known under the long-term pesticide stress in tea trees. In this study, we investigated the potential effects of foliar application of nanoselenium for a two-year field experiment on tea plants under pesticide-induced oxidative stress. Compared to control, nano-Se (10 mg/L) markedly enhanced the protein, soluble sugar, carotenoid, tea polyphenols, and catechins contents. High levels of theanine, glutamic acid, proline, and arginine were found to be induced most likely by adjusting the GS-GOGAT cycle. Se-supplementation may promote tea leaves’ secondary metabolism, thus increasing the accumulation of total phenols and flavonoids (apigenin, kaempferol, quercetin, myricetin, and rutin). It also minimized the accumulation of malondialdehyde, hydrogen peroxide, and superoxide anion by activating the antioxidants enzymes including in the AsA-GSH cycle. Selenium-rich tea also showed better fragrance and flavor. In summary, nano-Se can ameliorate the nutrients quality and abiotic stresses resistance of crops.
اظهر المزيد [+] اقل [-]Influential parameters on particle concentration and size distribution in the mainstream of e-cigarettes
2014
Fuoco, F.C. | Buonanno, G. | Stabile, L. | Vigo, P.
Electronic cigarette-generated mainstream aerosols were characterized in terms of particle number concentrations and size distributions through a Condensation Particle Counter and a Fast Mobility Particle Sizer spectrometer, respectively. A thermodilution system was also used to properly sample and dilute the mainstream aerosol.Different types of electronic cigarettes, liquid flavors, liquid nicotine contents, as well as different puffing times were tested. Conventional tobacco cigarettes were also investigated.The total particle number concentration peak (for 2-s puff), averaged across the different electronic cigarette types and liquids, was measured equal to 4.39 ± 0.42 × 109 part. cm−3, then comparable to the conventional cigarette one (3.14 ± 0.61 × 109 part. cm−3). Puffing times and nicotine contents were found to influence the particle concentration, whereas no significant differences were recognized in terms of flavors and types of cigarettes used.Particle number distribution modes of the electronic cigarette-generated aerosol were in the 120–165 nm range, then similar to the conventional cigarette one.
اظهر المزيد [+] اقل [-]Analysis of heavy metals and PAHs in the waste resulting from hookah consumption: Ahvaz City, Iran
2022
Dehvari, Mahboobeh | Babaei, AliAkbar
Hookah, a popular form of smoking tobacco, has been commonly used in Asia and Africa. However, the widespread consumption of hookah has a major impact on human health being. In addition to health risks, discarding the waste products of hookah would be a serious potential danger for environmental resources. In this study, the waste materials of hookah consumption (water, tobacco, and charcoal) were analyzed for the determination of the metals and PAHs using inductively coupled plasma mass spectrometry (ICP-MS) and gas chromatography-mass spectrometry (GC–MS), respectively. Results showed that the average concentration of iron in different flavors of tobacco had the highest amount (3654.2 ppb). Iron had the maximum concentration among the heavy metals, particularly in the mint flavor (10,863.3 ± 4.1). The highest ƩPAHs were related to the mint flavor (47.48 ± 15.21 ppb). Results showed that the aromatic tobacco available in the Ahvaz-Iran market is polluted by heavy metals. The high consumption of hookah in Ahvaz city and the presence of pollutants in hookah waste materials, apart from health risk aspects, can be potentially regarded as sources of environmental contamination. Therefore, education and preventive measures are necessary for this concern.
اظهر المزيد [+] اقل [-]Performance analysis of a novel thermal energy storage integrated solar dryer for drying of coconuts
2022
Radhakrishnan Govindan, Gopinath | Sattanathan, Muthuvel | Muthiah, Muthukannan | Ranjitharamasamy, Sudhakara Pandian | Athikesavan, Muthu Manokar
The drying of food products is an essential step in the preservation of crops and agricultural by-products that serve as raw materials for numerous end applications. Solar drying with phase change materials (PCMs) is an efficient low-energy consumption process in the post-reaping stage, reducing food deterioration. A customized solar dryer setup was assembled using Cudappah (black) stones as the base of the drying chamber to facilitate the absorption of solar energy on its surface. The organic paraffin wax, with a melting point of 60 ℃, was used as PCM in the solar dryer. The novelty of the study is the application of a PCM in a solar dryer to improve the effectiveness of drying and decrease the absolute drying period and the microbial content in the dried coconut. The study compared the drying characteristics between open sun drying and solar drying without and with PCM (100 and 200 g). The fabricated setup was utilised for drying coconut using a PCM-based solar drying method. The coconut was dried from an initial moisture content of 55.5% to a final moisture content of about 9%. The prototype dryer model minimized the use of the workforce, avoided improper drying, and decreased the absolute drying time. A total plate count (TPC) test was conducted to characterize the microbial content in the dried coconut. The microbial count decreased with the use of 200-g PCM as the use of PCM retained the heat for a longer time in the chamber. The drying time of coconut decreased by about 28 and 52 h on using 100 and 200 g of PCM, respectively, compared to open sun drying. The sensory characteristics like colour, taste, flavour, quality, and texture of the solar-dried coconut sample were superior to the sun-dried coconut sample.
اظهر المزيد [+] اقل [-]Risk assessment of heavy metals in rooibos (Aspalathus linearis) tea consumed in South Africa
2021
Areo, Oluwaseun Mary | Njobeh, Patrick Berka
A total of 80 rooibos tea samples from a range of brands were purchased from various registered retail shops in South Africa. The samples were bought during 2019 winter (40) and summer (40) period which are classified as 6 natural rooibos, 18 herbal rooibos samples, and 16 flavor rooibos samples and subjected for heavy metal analysis such as chromium (Cr), iron (Fe), arsenic (As), cadmium (Cd), and lead (Pb) using inductively coupled plasma mass spectrometer (ICP-MS). Human health risks were determined by estimating the daily intake non-cancer hazard quotient (THQ) and hazard index (HI) via oral exposure to toxic elements based on daily tea consumption. The concentration range of the determined heavy metals in rooibos tea samples were as follows: Cr (0.17–11.98 mg/kg), Fe (31–450 mg/kg), As (ND–0.51 mg/kg), Cd (0.09–0.17 mg/kg), and Pb (0.06–2.73 mg/kg). Cr was found in higher amount when compared to the World Health Organization (WHO) permissive limit (1.3 mg/kg). The concentrations of all studied heavy metals during winter and summer period were compared using two-way Anova, and no significant differences (p = 0.832) were observed for the two seasons. Both the target risk quotient (THQ) and the hazard index (HI) levels in all analyzed tea were well below 1, implying that intake of rooibos tea with analyzed heavy metals should not cause a threat to human health. On the other hand, the continuous intake due to the high concentrations of trace metals such as Cr may pose a serious chronic health risk due to accumulation in body tissues over time. The study, therefore, suggests constant monitoring of these heavy metals in teas in order to limit the risk of exceeding the permissive limits.
اظهر المزيد [+] اقل [-]Characteristics of flavored and non-flavored waterpipe tobacco users: a real-world setting study
2021
Ebrahimi Kalan, Mohammad | Abazari, Malek | Ben Taleb, Ziyad | Adham, Davoud | Abbasi, Abbas | Bursac, Zoran | Ward, Kenneth D. | Cobb, Caroline O | Behaleh, Raed | Wipfli, Heather Lynn | Fazlzadeh, Mehdi
We aimed to compare sociodemographic characteristics, smoking patterns, beliefs and perceptions, nicotine dependence, and psychological indicators between flavored waterpipe (WP) tobacco (FWT) and non-flavored WP tobacco (non-FWT) smokers in Iran. A cross-sectional study was conducted in 94 WP-serving venues surrounding Tehran and Ardabil metropolitans in Iran. Convenience sampling was applied to select 900 current WP smokers [508 (56%) FWT-only and 392 (44%) non-FWT-only smokers] aged 18 years and older. Multivariable logistic regression was used to examine the independent characteristics of FWT and non-FWT smokers. Compared to non-FWT smokers, FWT smokers were younger (adjusted odds ratio [AOR]=0.88, 95% confidence interval [CI]: 0.87–0.90) and more likely to have ≥ 1 sibling who smoked WP (AOR=1.54, 95% CI: 1.06–2.24), share WP with others (AOR=2.52, 95% CI: 1.68–3.77), report current cigarette smoking (AOR=2.05, 95% CI: 1.23–3.42), and report confidence in quitting at any time (AOR=3.64, 95% CI: 2.45–5.39). FWT smokers were less likely to have seen (AOR=0.40, 95% CI: 0.27–0.60) or read (AOR=0.44, 95% CI: 0.24–0.80) warning messages on WP tobacco packages relative to non-FTW smokers. The most common self-reported reason for smoking WP was entertainment for both groups, followed by stress relief for non-FWT smokers and the escape from loneliness for FWT smokers. FWT-only smokers differ from non-FWT-only smokers in several aspects including being younger, having more positive beliefs and perceptions regarding WP use, and having higher cigarette smoking prevalence. These findings will help in developing more effective and targeted policies and cessation interventions for WP smokers according to flavor type they use, especially in the Middle East.
اظهر المزيد [+] اقل [-]Towards a better understanding of the therapeutic applications and corresponding mechanisms of action of honey
2017
Khan, Rifat Ullah | Naz, Shabana | Abudabos, Alaeldein M.
Honey is a bee-derived supersaturated solution composed of complex contents mainly glucose, fructose, amino acids, vitamins, and minerals. Composition of honey may vary due to the difference in nectar, season, geography, and storage condition. Honey has been used since times immemorial in folk medicine and has recently been rediscovered as an excellent therapeutic agent. In the past, honey was used for a variety of ailments without knowing the scientific background and active ingredients of honey. Today, honey has been scientifically proven for its antioxidant, regulation of glycemic response, antitumor, antimicrobial, anti-inflammatory, and cardiovascular potentiating agent. It can be used as a wound dressing and healing substance. Honey is different in color, flavor, sensory perception, and medical response. Apart from highlighting the nutritional facts of honey, we collected the finding of the published literature to know the mechanism of action of honey in different diseases. This review covers the composition, physiochemical characteristics, and some medical uses.
اظهر المزيد [+] اقل [-]Sustainable application of cochineal-based anthraquinone dye for the coloration of bio-mordanted silk fabric
2020
Amin, Nimra | Rehman, Fazal-ur- | Adeel, Shahid | Ahamd, Tanvir | Muneer, Majid | Haji, Aminoddin
Natural colors particularly animal-based colorants are employed in the field of cosmetics, food, and flavors and also gaining popularity in textiles, due to their soothing nature. In this study, the microwave-assisted extraction of colorant from cochineal insects for dyeing of bio-mordanted silk has been carried out. Acidic, methanolic, and acidified methanol solubilized media were used to extract the natural colorant from cochineal under microwave irradiation for 1–6 min. Bio-mordants have been employed at optimized conditions to make the process greener and sustainable. It is found that acid solubilized extract of pH 4, employed at 55 °C for 55 min containing 5 g/100 mL of Glauber’s salt as exhausting agent has given high color strength onto microwave-treated silk fabric. Suggested ISO standards for colorfastness have revealed that bio-mordants have given excellent color depth and excellent rating of fastness properties, compared with chemical mordants used. It is found that microwave treatment has not only improved the dyeing behavior of colorant extracted from cochineal in acid solubilized medium but also enhanced the color characteristics onto bio-mordanted silk fabric.
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