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Rhizophagus irregularis enhances tolerance to cadmium stress by altering host plant hemp (Cannabis sativa L.) photosynthetic properties
2022
Sun, Simiao | Feng, Yuhan | Huang, Guodong | Zhao, Xu | Song, Fuqiang
Arbuscular mycorrhizal fungi (AMF) are widespread and specialized soil symbiotic fungi, and the establishment of their symbiotic system is of great importance for adversity adaptation. To reveal the growth and photosynthetic characteristics of AMF–crop symbionts in response to heavy metal stress, this experiment investigated the effects of Rhizophagus irregularis (Ri) inoculation on the growth, photosynthetic gas exchange parameters, and chlorophyll fluorescence characteristics of hemp (Cannabis sativa L.) at a Cd concentration of 80 mg/kg. The results showed that (1) under Cd stress, the biomass of each plant structure in the Ri treatment was significantly higher than that in the noninoculation treatment (P < 0.05); (2) under Cd stress, the transpiration rate, stomatal conductance, net photosynthetic rate, PSII efficiency, apparent electron transport rate and photochemical quenching coefficient of the Ri inoculation group reached a maximum, with increases ranging from 1% to 28%; (3) inoculation of Ri significantly reduced Cd enrichment in leaves, which in turn significantly increased the transpiration rate, stomatal conductance, electron transfer rate, net photosynthetic rate and photosynthetic intensity, protecting PSII (P < 0.05); and (4) by measuring the light response curves of different treatments, the light saturation points of hemp inoculated with the Ri treatment reached 1448.4 μmol/m²/s, and the optical compensation point reached 24.0 μmol/m²/s under Cd stress. The Ri–hemp symbiont demonstrated high adaptability to weak light and high utilization efficiency of strong light under Cd stress. Our study showed that Ri–hemp symbiosis improves adaptation to Cd stress and promotes plant growth by regulating the photosynthetic gas exchange parameters and chlorophyll fluorescence parameters of plants. The Ri–hemp symbiosis is a promising technology for improving the productivity of Cd-contaminated soil.
اظهر المزيد [+] اقل [-]Historical mercury contamination in sediments and catchment soils of Diss Mere, UK
2010
Yang, Handong
A 5.3 m sediment core and soil samples were taken from Diss Mere and its catchment. The sediment core was dated and Hg analysed on the sediment and soil samples. The Hg record of the sediment core shows that Diss Mere has been contaminated for the past thousand years and the historical trends in sediment contamination are in good agreement with the development of the weaving industry in Diss and hemp cultivation in the region. Mercury contamination in Diss Mere has been significant and reached a peak in the mid-19th century with sediment Hg concentrations over 50 μg g-1. Elevated Hg concentrations were also found in contemporary soils in residential areas with former industrial land use. Although local hemp cultivation and the traditional weaving industry were abandoned a hundred years ago, Hg contamination caused by these activities still exists in the catchment, and affects the lake.
اظهر المزيد [+] اقل [-]Silicon reduces cadmium absorption and increases root-to-shoot translocation without impacting growth in young plants of hemp (Cannabis sativa L.) on a short-term basis
2021
Luyckx, Marie | Hausman, Jean-François | Blanquet, Mathilde | Guerriero, Gea | Lutts, Stanley
Textile hemp (Cannabis sativa L.) is a non-edible multipurpose crop suitable for fiber production and/or phytoremediation on moderately heavy metal–contaminated soils. Experiments were conducted in nutrient solution to assess the short-term impact of silicon (Si), a well-known beneficial element, on plants exposed to 20 μM cadmium (Cd) in nutrient solution. Cd decreased plant growth and affected photosynthesis through non-stomatal effects. Cd translocation factor was higher than 1, confirming the interest of hemp for phytoextraction purposes. Additional Si did not improve plant growth after 1 week of treatment but decreased Cd accumulation in all organs and improved water use efficiency through a decrease in transpiration rate. Si had only marginal impact on Cd distribution among organs. It increased glutathione and phytochelatin synthesis allowing the plants to efficiently cope with oxidative stress through the improvement of Cd sequestration on thiol groups in the roots. Si may thus have a fast impact on the plant behavior before the occurrence of plant growth stimulation.
اظهر المزيد [+] اقل [-]Evaluation of hemp (Cannabis sativa L.) as an industrial crop: a review
2021
Rehman, Muzammal | Shah, Fahad | Du, Guanghui | Cheng, Xia | Yang, Yang | Tang, Kailei | Liu, Lijun | Liu, Fei-Hu | Deng, Gang
Rising human population has increased the utilization of available resources for food, clothes, medicine, and living space, thus menacing natural environment and mounting the gap between available resources, and the skills to meet human desires is necessary. Humans are satisfying their desires by depleting available natural resources. Therefore, multifunctional plants can contribute towards the livelihoods of people, to execute their life requirements without degrading natural resources. Thus, research on multipurpose industrial crops should be of high interest among scientists. Hemp, or industrial hemp, is gaining research interest because of its fastest growth and utilization in commercial products including textile, paper, medicine, food, animal feed, paint, biofuel, biodegradable plastic, and construction material. High biomass production and ability to grow under versatile conditions make hemp, a good candidate species for remediation of polluted soils also. Present review highlights the morphology, adaptability, nutritional constituents, textile use, and medicinal significance of industrial hemp. Moreover, its usage in environmental conservation, building material, and biofuel production has also been discussed.
اظهر المزيد [+] اقل [-]In situ growth of carbon nitride on titanium dioxide/hemp stem biochar toward 2D heterostructured photocatalysts for highly photocatalytic activity
2020
Peng, Xiaoming | Wang, Min | Dai, Hongling | Qiu, Fengxian | Hu, Fengping
In this work, hierarchical structure TiO₂/hemp stem biochar carbon (HSBC) and C₃N₄-TiO₂/HSBC were successfully fabricated, which were used as efficient visible-light photocatalyst degradation for ammonia nitrogen from aqueous solution. The as-prepared C₃N₄-TiO₂/HSBC hybrid catalyst showed the higher efficient photocatalytic activity for decomposition of ammonia nitrogen than those of pure TiO₂ and TiO₂/HSBC, suggesting suppressed recombination of photogenerated charges and promoted mass transfer due to synergistic effect, and thus increased photocatalytic degradation activity. The degradation of ammonia follows a pseudo-first-order kinetics. All prepared catalysts demonstrated extremely photocatalytic efficiency under visible-light and UV light illumination; the ammonia nitrogen photocatalytic degradation activity of C₃N₄-TiO₂/HSBC can reach 90.3% under UV light while the degradation activity achieved about 50.7% under visible-light irradiation. The results revealed that the h⁺ was dominantly active intermediates in the process of photocatalytic degradation. The prepared catalysts are promising for the degradation of ammonia nitrogen from water resource.
اظهر المزيد [+] اقل [-]Single and combined use of Cannabis sativa L. and carbon-rich materials for the removal of pesticides and endocrine-disrupting chemicals from water and soil
2021
Loffredo, Elisabetta | Picca, Giuseppe | Parlavecchia, Marco
Hemp (Cannabis sativa L.) seedlings were used to remove from water the fungicide metalaxyl-M and the endocrine disruptor (EDC) bisphenol A (BPA) at concentrations ranging from 2 to 100 μg mL⁻¹. In 7 days of exposure, despite the phytotoxicity of each compound that reduced elongation and biomass, the seedlings were able to remove between 67 and 94% of metalaxyl-M and between 86 and 95% of BPA. The amounts of metalaxyl-M and BPA extracted from plant dry biomass were in the range of 106–3861 μg g⁻¹ and 16–101 μg g⁻¹, respectively, and resulted positively correlated to both the dose of compound added (P ≤ 0.01) and the amount removed by the plants (P ≤ 0.01). Plant uptake and transformation were the main mechanisms involved in the removal of the compounds. In another set of experiments, hemp was used to remove a mixture of two pesticides, metalaxyl-M and metribuzin, and three EDCs, BPA, 17β-estradiol (E2), and 4-tert-octylphenol (OP), at concentrations of 10, 10, 10, 10, and 1 μg g⁻¹, respectively, from soil column not added and added with 2.5% (w/w) of a green compost (CM) or a wood biochar (BC). In 25 days, plants did not alter considerably the distribution of the compounds along the soil profile and were capable of removing, on average, 12, 11, 10, 9, and 14% of metalaxyl-M, metribuzin, BPA, E2, and OP, respectively. During growth, hemp transformed the compounds and accumulated part of them (except OP) mainly in the shoots. CM and, especially, BC significantly protected the plants from the toxicity of the compounds and enhanced the retention of the latter in soil, contrasting leaching. Thus, the single or synergistic use of hemp and amendments deserves attention being a very low-cost and eco-sustainable strategy to remediate water and soil.
اظهر المزيد [+] اقل [-]As(V)/Cr(VI) retention on un-amended and waste-amended soil samples: competitive experiments
2017
Rivas-Pérez, Ivana M. | Conde-Cid, Manuel | Nóvoa-Muñoz, Juan Carlos | Arias-Estévez, Manuel | Fernández-Sanjurjo, María J. | Álvarez-Rodríguez, Esperanza | Núñez Delgado, Avelino
Focusing on simultaneous arsenic and chromium pollution, we used batch-type experiments to study As(V)/Cr (VI) competitive sorption on soil samples, pyritic material, mussel shell, oak ash, pine bark and hemp waste, as well as on binary mixtures (50 % mussel shell and 50 % another material—oak ash, pine bark, or hemp waste), and on forest and vineyard soil samples and pyritic material amended with 48 t ha⁻¹ of mussel shell, oak ash, pine bark, or hemp waste. Equal As(V) and Cr(VI) concentrations (0 to 6 mmol L⁻¹) were added to the individual materials, binary mixtures, and 48 t ha⁻¹ amended materials. The individual forest soil sample, pyritic material, and oak ash showed clearly higher As(V) sorption, whereas Cr(VI) sorption was higher on pine bark. Sorption was up to 50 % higher for As(V) than for Cr(VI) on the forest soil sample, oak ash, and pyritic material, while pine bark sorbed 95 % more Cr(VI). Regarding binary mixtures, the presence of mussel shell increased As(V) sorption on pine bark and Cr(VI) sorption on hemp waste. As regards the amendments, in the case of the forest soil sample, the amendments with oak ash and mussel shell increased As(V) sorption, while pine bark amendment increased Cr(VI) sorption; in the vineyard soil sample, the mussel shell amendment increased As(V) sorption; in the pyritic material, pine bark amendment increased Cr(VI) sorption. These results could be useful to appropriately manage the soils and individual or mixed by-products assayed when As(V) and Cr(VI) pollution occurs.
اظهر المزيد [+] اقل [-]Effect of hydrogen addition on performance, emission, and combustion characteristics of Deccan hemp oil and its methyl ester–fuelled CI engine
2019
Jayaraman, Kamalakannan | Babu, Geetha Narayanasamy | Dhandapani, Gopinath | Varuvel, Edwin Geo
A vegetable oil–fueled diesel engine operation is characterized by low brake thermal efficiency and relatively high smoke emission. Conversion of vegetable oil to biodiesel results in slight improvement in efficiency and smoke emission, but the values are not comparable with diesel. In this work, a single-cylinder diesel engine’s performance is evaluated by inducting hydrogen in small quantities in the intake manifold along with Deccan hemp oil (DHO) and its methyl ester (DHOME) as the pilot fuel. The tests were conducted at part-load and full-load conditions at an engine speed of 1500 rpm. Results indicate an increase in brake thermal efficiency from 29.7 to 32.6% and from 27.3 to 29.6% at full load with hydrogen-induced DHOME and DHO engine operation. Unburned-hydrocarbon emissions, carbon monoxide emission, and smoke emission reduced for both the fuels. However, NOx levels increased for the two fuels because hydrogen induction causes high combustion rates and high temperature in the combustion chamber. Hydrogen induction leads to high premixed combustion resulting in high peak pressures.
اظهر المزيد [+] اقل [-]The crop-residue of fiber hemp cv. Futura 75: from a waste product to a source of botanical insecticides
2018
In the attempt to exploit the potential of the monoecious fiber hemp cv. Futura 75 in new fields besides textile, cosmetics and food industry, its crop-residue given by leaves and inflorescences was subjected to hydrodistillation to obtain the essential oils. These are niche products representing an ideal candidate for the development of natural insecticides for the control and management of mosquito vectors, houseflies and moth pests. After GC-MS analysis highlighting a safe and legal chemical profile (THC in the range 0.004–0.012% dw), the leaf and inflorescence essential oils were investigated for the insecticidal potential against three insect targets: the larvae of Culex quinquefasciatus and Spodoptera littoralis and the adults of Musca domestica. The essential oil from inflorescences, showing (E)-caryophyllene (21.4%), myrcene (11.3%), cannabidiol (CBD, 11.1%), α-pinene (7.8%), terpinolene (7.6%), and α-humulene (7.1%) as the main components, was more effective than leaf oil against these insects, with LD₅₀ values of 65.8 μg/larva on S. littoralis, 122.1 μg/adult on M. domestica, and LC₅₀ of 124.5 μl/l on C. quinquefasciatus larvae. The hemp essential oil moderately inhibited the acetylcholinesterase (AChE), which is a target enzyme in pesticide science. Overall, these results shed light on the future application of fiber hemp crop-residue for the development of effective, eco-friendly and sustainable insecticides.
اظهر المزيد [+] اقل [-]As(V)/Cr(VI) pollution control in soils, hemp waste, and other by-products: competitive sorption trials
2016
Quintáns-Fondo, Ana | Ferreira-Coelho, Gustavo | Paradelo-Núñez, Remigio | Nóvoa-Muñoz, Juan Carlos | Arias-Estévez, Manuel | Fernández-Sanjurjo, María José | Álvarez-Rodríguez, Esperanza | Núñez Delgado, Avelino
We study As(V)/Cr(VI) competitive sorption on a forest soil, a vineyard soil, pyritic material, mussel shell, pine bark, oak ash, and hemp waste, adding variable As(V) and Cr(VI) concentrations or displacing each pollutant with the same concentration of the other. When using variable concentrations, As(V) showed more affinity than Cr(VI) for sorption sites on most materials (sorption up to >84 % on oak ash and pyritic material). The only exception was pine bark, with clearly higher Cr(VI) sorption (>90 %) for any Cr(VI)/As(V) concentration added. Regarding the displacement experiments, when As(V) was added and reached sorption equilibrium, the subsequent addition of equal Cr(VI) concentration did not cause relevant As displacement from oak ash and pyritic material, indicating strong As bindings, and/or low competitive effects. When Cr(VI) was added and reached sorption equilibrium, the subsequent addition of equal As(V) concentration caused Cr(VI) displacement from all materials except pine bark, indicating weak Cr bindings. In view of these results, oak ash and the pyritic material could be used to remove As(V) in concentrations as high as 6 mmol L⁻¹, even in the presence of a wide range of Cr(VI) concentrations, whereas pine bark could be used to remove Cr(VI) concentrations as high as 6 mmol L⁻¹. The other materials assayed (including hemp waste, studied for the first time as As(V) and Cr(VI) bio-sorbent) cannot be considered appropriate to remove As(V) and/or Cr(VI) from polluted media.
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