خيارات البحث
النتائج 1 - 10 من 35
Microplastic particles increase arsenic toxicity to rice seedlings
2020
Dong, Youming | Gao, Minling | Song, Zhengguo | Qiu, Weiwen
Hydroponic experiments were conducted to study the effects of microplastic particles of polystyrene (PS) and polytetrafluoroethylene (PTFE) on arsenic (As) content in leaves and roots of rice seedlings, and the changes in root vigor and physiological and biochemical indicators under single or combined PS and PTFE with As(III) treatment. Rice biomass decreased with increasing concentrations of PS, PTFE, and As(III) in the growth medium. The highest root (leaf) biomass decreases were 21.4% (10.2%), 25.4% (11.8%), and 26.2% (16.2%) with the addition of 0.2 g L⁻¹ PS, 0.2 g L⁻¹ PTFE, and 4 mg L⁻¹ As(III), respectively. Microplastic particles and As(III) inhibited biomass accumulation by inhibiting root activity and RuBisCO activity, respectively. The addition of As(III) and microplastic particles (PS or PTFE) inhibited photosynthesis through non-stomatal and stomatal factors, respectively; furthermore, net photosynthetic rate, chlorophyll fluorescence, and the Chl a content of rice were reduced with the addition of As(III) and microplastic particles (PS or PTFE). Microplastic particles and As(III) induced an oxidative burst in rice tissues through mechanical damage and destruction of the tertiary structure of antioxidant enzymes, respectively, thereby increasing O₂⁻ and H₂O₂ in roots and leaves, inducing lipid peroxidation, and destroying cell membranes. When PS and PTFE were added at 0.04 and 0.1 g L⁻¹, respectively, the negative effects of As(III) on rice were reduced. Treatment with 0.2 g L⁻¹ PS or PTFE, combined with As(III), had a higher impact on rice than the application of As(III) alone. PS and PTFE reduced As(III) uptake, and absorbed As decreased with the increasing concentration of microparticles. The underlying mechanisms for these effects may involve direct adsorption of As, competition between As and microplastic particles for adsorption sites on the root surface, and inhibition of root activity by microplastic particles.
اظهر المزيد [+] اقل [-]Contrasting effects of Cr(III) and Cr(VI) on lettuce grown in hydroponics and soil: Chromium and manganese speciation
2020
Park, Jin Hee
Chromium (Cr) is a toxic element among which hexavalent chromium [Cr(VI)] is more toxic than trivalent chromium [Cr(III)]. Chromium can be reduced or oxidized in soil because soil is a complex medium and various soil components affect redox reaction of Cr in soil. Therefore, Cr speciation in hydroponics and soil was compared and Cr uptake and speciation by lettuce grown in the media were evaluated. Higher phytotoxicity was found in Cr(III) spiked soil than in Cr(VI) spiked soil, while Cr toxicity was higher in Cr(VI) treated hydroponics than Cr(III) treated hydroponics. Chromium was mainly accumulated in lettuce roots as Cr(III), and more Cr was translocated from roots to shoots grown in Cr(VI) treated hydroponics than Cr(III) treated hydroponics. Accumulation of Cr in roots grown in Cr(III) treated nutrient solution reduced Fe, K, Ca, Mg, and P uptake in lettuce. Chromium valence state was Cr(III) in lettuce leaves and roots grown in both Cr(III) and Cr(VI) treated hydroponics and soil. Chromium speciation in hydroponically grown lettuce roots was Cr(III) coordinated with 6 oxygens in the first shell and 2 or 4 carbons in the second shell as analyzed by X-ray absorption spectroscopy (XAS), which was similar to chromium acetate. The valence state of Cr in Cr(III) and Cr(VI) treated nutrient solution was not changed, while Cr(VI) was reduced to Cr(III) in Cr(VI) spiked soil by soil organic matter. Spiking of Cr(III) induced reduction of Mn in soil, which resulted in an increase of bioavailable Mn concentration in the Cr(III) spiked soil. Therefore, the increased phytotoxic effect for lettuce in Cr(III) spiked soil can be attributed to the reduction of Mn and subsequent release of Mn(II). For Cr(III) contaminated soil, Mn speciation should be considered, and bioavailable Mn concentration should be monitored although Cr existed as Cr(III) in soil.
اظهر المزيد [+] اقل [-]Biotransformation of 6:2 fluorotelomer alcohol by the whole soybean (Glycine max L. Merrill) seedlings
2020
Zhang, Hongna | Wen, Bei | Huang, Honglin | Wang, Sen | Cai, Zongwei | Zhang, Shuzhen
Fluorotelomer alcohols (FTOHs) are important precursors of perfluorocarboxylic acids (PFCAs) in the environment and biota. With the growing application of 6:2 FTOH [F(CF₂)₆CH₂CH₂OH] in product formulation, it is becoming increasingly urgent to investigate its biological fates in different species. In this study, biotransformation of 6:2 FTOH by young soybean plants (Glycine max L. Merrill) were investigated using hydroponic experiments. During the 144 h-exposure, 6:2 FTCA [F(CF₂)₆CH₂COOH], 6:2 FTUCA [F(CF₂)₅CFCHCOOH], 5:3 FTUCA [F(CF₂)₅CHCHCOOH], 5:3 FTCA [F(CF₂)₅CH₂CH₂COOH], PFHxA [F(CF₂)₅COOH] and PFPeA [F(CF₂)₄COOH] were phase I metabolites in soybean. At the end of exposure, 5:3 FTCA (5.08 mol%), PFHxA (2.34 mol%) and PFPeA (0.58 mol%) were three main metabolites in soybean-solution system. 5:3 FTCA was predominant in soybean roots and stems, while PFHxA was the most abundant product in leaves. PFBA [F(CF₂)₃COOH] and 4:3 FTCA [F(CF₂)₄CH₂CH₂COOH] detected in the hydroponic solution most-likely came from the transformation of 5:3 FTCA by root-associated microbes. Moreover, phase II metabolites of 6:2 FTOH were identified and monitored in soybean tissues. Alcohol dehydrogenase, aldehyde dehydrogenase and glutathione S-transferase were found to participate in 6:2 FTOH metabolism. Based on the phase I and phase II metabolism of 6:2 FTOH in soybean, this study for the first time provides evidences for the transformation pathways of 6:2 FTOH in plants.
اظهر المزيد [+] اقل [-]The effect of an antimony resistant bacterium on the iron plaque fraction and antimony uptake by rice seedlings
2020
Long, Jiumei | Zhou, Dongsheng | Li, Bingyu | Zhou, Yimin | Li, Yongjie | Lei, Ming
Iron plaque (IP) is crucial in mitigating antimony (Sb) uptake and accumulation in rice plants, while, few studies focused on the effect of the iron plaque-associated Sb resistant bacteria on IP and Sb uptake into rice plants. Here, the effect of a Sb resistant bacterium (GenBank accession No. MH345840, with potential of conversion soluble Sb(III) into insoluble Sb₂O₃) on IP and Sb(III)/Sb(V) uptake under hydroponic condition was investigated. The results showed that in the presence of Sb(III), a large quantity of bacterial cells consorted with IP on rice roots, the bacterial inoculum altered the IP fraction distribution without enhancing its amount. However, it reduced Sb(III) uptake into rice roots. On contrary, seldom bacterial cells associated with the IP on rice roots in the presence of the Sb(V), the bacterial inoculum increased the IP amount slightly, and did not decline the Sb(V) uptake into rice roots. It also showed that the bacterial inoculum decreased Sb concentrations in rice shoots greatly in both Sb(III) and Sb(V) supplied treatments.
اظهر المزيد [+] اقل [-]Uptake, translocation, and physiological effects of hematite (α-Fe2O3) nanoparticles in barley (Hordeum vulgare L.)
2020
Tombuloglu, Huseyin | Slimani, Yassine | AlShammari, Thamer Marhoon | Bargouti, Muhammed | Ozdemir, Mehmet | Tombuloglu, Guzin | Ak̲h̲tar, Sult̤ān | Sabit, Hussain | Hakeem, Khalid Rehman | Almessiere, Munirah | Ercan, İsmail | Baykal, Abdulhadi
There has been a growing concern with the environmental influences of nanomaterials due to recent developments in nanotechnology. This study investigates the impact and fate of hematite nanoparticles (α-Fe₂O₃ NPs) (∼14 nm in size) on a crop species, barley (Hordeum vulgare L.). For this purpose, hematite NPs (50, 100, 200, and 400 mg/L) were hydroponically applied to barley at germination and seedling stages (three weeks). Inductively coupled plasma mass spectrophotometry (ICP-MS) along with vibrating sample magnetometer (VSM) techniques were used to track the NPs in plant tissues. The effects of NPs on the root cells were observed by scanning electron microscopy (SEM) and confocal microscopy. Results revealed that α-Fe₂O₃ NPs significantly reduced the germination rate (from 80% in control to 30% in 400 mg/L), as well as chlorophyll (36–39%) and carotenoid (37%) contents. Moreover, the treatment led to a significant decline in the quantum yield of photosystem II (Fv/Fm). Leaf VSM analysis indicated a change in magnetic signal for NPs-treated samples compared with untreated ones, which is mostly attributed to the iron (Fe) ions incorporated within the leaf tissue. Besides, Fe content in the roots and leaf had gradually increased by the increasing doses of NPs, which was confirming NPs’ translocation to the aerial parts. Microscopic observations revealed that α-Fe₂O₃ NPs altered root cell morphology and led to the injury of cell membranes. This study, in the light of our findings, shows that α-Fe₂O₃ NPs (∼14 nm in size) are taken up by the roots of the barley plants, and migrate to the plant leaves. Besides, NPs are phytotoxic for barley as they inhibit germination and pigment biosynthesis. This inhibition is probably due to the injury of the cell membranes in the roots. Therefore, the use of hematite NPs in agriculture and thereby their environmental diffusion must be addressed carefully.
اظهر المزيد [+] اقل [-]Hydroponic growth test of maize sprouts to evaluate As, Cd, Cr and Pb translocation from mineral fertilizer and As and Cr speciation
2020
Fioroto, Alexandre M. | Albuquerque, Luiza G.R. | Carvalho, Alexandrina A.C. | Oliveira, Aline P. | Rodrigues, Fábio | Oliveira, Pedro V.
The present study proposes a maize sprouts hydroponic growth model to evaluate the As, Cd, Cr and Pb translocation from multinutrient fertilizer and to do speciation of As and Cr in this fertilizer and As in parts of plant in order to predict their phytoavailability. X-ray absorption near edge structure (XANES) was employed to speciate As and Cr directly on fertilizer solid sample. Arsenate (Asⱽ) and a solid solution of FeCrO₃ were the major species identified in the samples. The sprouts were hydroponically cultivated in water, fertilizer slurry and fertilizer extract media. Concentrations of As, Cd and Pb measured on leaves of maize sprouts ranged from 0.061 to 0.31 mg kg⁻¹, whereas Cr was not translocated to the aerial parts of sprouts. High performance liquid chromatographic with inductively coupled plasma mass spectrometry (HPLC-ICP-MS) analysis was used to determine As speciation in maize sprouts, as well as in the fertilizer extracts and slurries. Arsenate was the only species identified in the initial fertilizer extract and this information is in agreement with the XANES results. However, the reduction of arsenate to arsenite was observed in extracts and slurries collected after sprout growth, probably due to the action of exudates secreted by plant roots. Arsenite was the predominant species identified in sprouts, the high phosphate concentration in the medium may have contributed to reduce arsenate phytoavailability.
اظهر المزيد [+] اقل [-]Uptake, translocation and accumulation of the fungicide benzene kresoxim-methyl in Chinese flowering cabbage (Brassica campastris var. parachinensis) and water spinach (Ipomoea aquatica)
2020
Chen, Yan | Lu, Yuhui | Nie, Enguang | Akhtar, Kashif | Zhang, Subin | Ye, Qingfu | Wang, Haiyan
Benzene kresoxim-methyl (BKM) is an important methoxyacrylate-based strobilurin fungicide widely used against various phytopathogenic fungi in crops. Uptake, translocation and accumulation of BKM in vegetables remain unknown. This study was designed to investigate uptake, translocation, and accumulation of ¹⁴C-BKM and/or its potential metabolites in Chinese flowering cabbage and water spinach. ¹⁴C-BKM can be gradually taken up to reach a maximum of 44.4% of the applied amount by Chinese flowering cabbage and 34.6% by water spinach at 32 d after application. The ¹⁴CO₂ fractions released from the hydroponic plant system reached 37.8% for cabbage and 45.8% for water spinach, respectively. Concentrations of ¹⁴C in leaves, stems and roots all gradually increased as vegetables growing, with relative 44.9% (cabbage) and 26.8% (water spinach) of translocated from roots to edible leaves. In addition, ¹⁴C in leaves was mainly accumulated in the bottom leaves, which was visualized by quantitative radioautographic imaging. The bioconcentration factor of ¹⁴C ranged from 7.1 to 38.2 mL g⁻¹ for the cabbage and from 8.6 to 24.6 mL g⁻¹ for the water spinach. The translocation factor of BKM ranged from 0.10 to 2.04 for the cabbage and 0.10–0.46 for the water spinach throughout the whole cultivation period, indicating that the cabbage is easier to translocate BKM from roots to leaves and stems than water spinach. In addition, the daily human exposure values of BKM in both vegetables were much lower than the limited dose of 0.15 mg day⁻¹. The results help assess potential accumulation of BMK in vegetables and potential risk.
اظهر المزيد [+] اقل [-]Metal accumulation and rhizosphere characteristics of Noccaea rotundifolia ssp. cepaeifolia
2020
Golestanifard, Alireza | Puschenreiter, Markus | Aryan, Amal | Santner, Jakob | Wenzel, Walter W.
This work aimed to investigate the metal accumulation characteristics as well as biogeochemical changes in the rhizosphere and root foraging strategies of this plant species. Previous reports suggested that Noccaea rotundifolia ssp. cepaeifolia is a Zn, Cd and Pb hyperaccumulator. We used hydroponic, rhizobox and split-pot experiments for studying metal accumulation and related rhizosphere processes. Although this species accumulated up to 1250 mg Pb kg⁻¹ and 27,000 mg Zn kg⁻¹ in shoots, translocation factors <1 do not meet the hyperaccumulation criteria. Substantial increases in Ca(NO₃)₂-extractable metals in the N. rotundifolia rhizosphere of a metal-spiked soil can be explained by proton release from N. rotundifolia roots to maintain the charge balance during excessive metal uptake; this was not observed for the non-spiked, moderately contaminated control soil. Specific rhizosphere mechanisms targeting the alleviation of metal toxicity in N. rotundifolia rhizosphere were not detected. Generally, N. rotundifolia had larger total root and shoot mass in soils with heterogeneous distribution of Zn and Pb relative to homogeneous treatments, associated with less root mass placed in metal-enriched patches. However, the avoidance strategy was not reflected by low shoot metal concentrations. Metal accumulation rates and translocation factors do not meet the criteria for hyperaccumulation. Changes of pH and DOC in N. rotundifolia rhizosphere were apparently not involved in targeted immobilisation or detoxification of Pb, Zn and Cd. Avoidance of metal-rich patches in soil is a major tolerance strategy of N. rotundifolia.
اظهر المزيد [+] اقل [-]The role of root apoplastic barriers in cadmium translocation and accumulation in cultivars of rice (Oryza sativa L.) with different Cd-accumulating characteristics
2020
Qi, Xiaoli | Tam, Nora Fung-yee | Li, Wai Chin | Ye, Zhihong
The radial translocation of cadmium (Cd) from the root to the shoot is one of the major processes affecting Cd accumulation in rice (Oryza sativa L.) grains, but few studies have focused on Cd apoplastic transport in rice. The aim of this study was to determine how apoplastic barriers affect Cd translocation via the apoplastic pathway, Cd accumulation levels in upper parts (shoot and grains) of rice cultivars, and the possible mechanism involved. Hydroponic and soil pot trials were conducted to study the development and chemical constituents of apoplastic barriers and their permeability to bypass flow, and to determine Cd localization in the roots of rice cultivars with different Cd-accumulating characteristics. The Cd accumulation in upper parts was positively correlated with bypass flow in the root and the apparent Cd concentration in the xylem, indicating that the apoplastic pathway may play an important role in Cd root-shoot translocation in rice. Apoplastic barriers were deposited closer to the root tip and were thicker in low Cd-accumulating cultivars than in high Cd-accumulating cultivars. The amounts and rates of increase in lignin and suberin were significantly higher in ZD14 (a low Cd-accumulating cultivar) than in FYXZ (a high Cd-accumulating cultivar) under Cd stress, indicating that stronger barriers were induced by Cd in ZD14. The stronger and earlier formation of barriers in the low Cd-accumulating cultivar decreased bypass flow more efficiently, so that more Cd was retained in the root during apoplastic translocation. This was confirmed by localization analyses of Cd in root transverse sections. These results suggest that apoplastic barriers reduce Cd root-to-shoot translocation via the apoplastic pathway, leading to lower Cd accumulation in the upper parts of rice plants. Bypass flow may have the potential to be used as a rapid screening indicator for low Cd-accumulating rice cultivars.
اظهر المزيد [+] اقل [-]Contrasting effects of iron plaque on the bioavailability of metallic and sulfidized silver nanoparticles to rice
2020
Wu, Yun | Yang, Lei | Gong, Hua | Dang, Fei | Zhou, Dong-Mei
Interaction between silver nanoparticles (AgNPs) and iron plaque, which forms at the root surface of wetland plants under waterlogging conditions, is a critical process that controls the bioavailability of AgNPs. In this study, we comparatively evaluated how and to what extent iron plaque affected silver uptake sourced from metallic (Ag⁰NPs) and sulfidized (Ag₂S-NPs) silver nanoparticles under hydroponic conditions. After the formation of iron plaque at the root surface upon exposure to Fe²⁺ at 0–100 μg mL⁻¹, rice (Oryza sativa L.) seedlings were transferred to AgNP suspensions. Silver uptake depended on the amount of iron plaque and AgNP species (Ag⁰NPs vs. Ag₂S-NPs): Ag₂S-NP exposure had lower or comparable Ag uptake to that of Ag⁰NP exposure at low levels of Fe²⁺ (0–80 μg mL⁻¹), but significantly higher Ag uptake at 100 μg Fe²⁺ mL⁻¹. Such contrasting effects of iron plaque on the bioavailability of Ag⁰NPs and Ag₂S-NPs were attributed to their influences on AgNP dissolution. However, the translocation factors (TFs) and particle size distribution of NPs in planta (as determined by single-particle inductively coupled plasma-mass spectrometry analysis) were not affected by the amount of iron plaque. These results reveal contrasting effects of iron plaque on the bioavailability of Ag⁰NPs and Ag₂S-NPs, and raise concerns about the exposure of wetland plants to Ag₂S-NPs in Fe-rich environments, where high Fe levels may facilitate Ag₂S-NP bioavailability.
اظهر المزيد [+] اقل [-]