The potential of cassava (Manihot esculenta Crantz.) for simultaneous Hg and Au phytoextraction was explored by investigating Hg and Au localization in cassava roots through Micro-Proton Induced X-Ray Emission, High-Resolution Transmission Electron Microscopy (HR-TEM) and X-Ray Diffractometry (XRD). The effect of Hg and Au in the cyanogenic glucoside linamarin distribution was also investigated using Matrix Assisted Laser Desorption Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (MALDI-FT-ICR-MS) imaging. Hg was located mainly in the root vascular bundle of plants grown in 50 or 100 μmol L⁻¹ Hg solutions. Au was localized in the epidermis and cortex or in the epidermis and endodermis for 50 and 100 μmol L⁻¹ Au solutions, respectively. For 50 μmol L⁻¹ solutions of both Hg and Au, the two metals were co-localized in the epidermis. When the Hg concentrations were increased to 100 μmol L⁻¹, Au was still localized to a considerable extent in the epidermis while Hg was located in all root parts. HR-TEM and XRD revealed that Au nanoparticles were formed in cassava roots. MALDI-FT-ICR-MS imaging showed linamarin distribution in the roots of control and plants and metal-exposed plants thus suggesting that linamarin might be involved in Hg and Au uptake and distribution.

Due to its high adaptability, cassava (Manihot esculenta Crantz) is one of the world’s most cultivated and consumed plants after maize and rice. However, there are relatively few scientific studies on this important crop. The objective of this review was therefore to summarize and discuss the available information on cassava cropping in order to promote sustainable practices in terms of production and consumption. Cassava cultivation has been expanding recently at the global scale and is widely consumed in most regions of South America, Africa, and Asia. However, it is also characterized by the presence in its roots of potentially toxic hydrocyanic acid. Furthermore, cassava can also absorb pollutants as it is currently cultivated near roads or factories and generally without consideration for potential sources of soil, water, or atmospheric pollution. Careful washing, peeling, and adequate preparation before eating are therefore crucial steps for reducing human exposure to both environmental pollutants and natural hydrocyanic acid. At present, there is not enough precise data available on this staple food crop. To improve our knowledge on the nutritive benefits versus health risks associated with cassava consumption, further research is necessary to compare cassava cultivars and precisely study the influence of preparation methods.