The present study proposes a maize sprouts hydroponic growth model to evaluate the As, Cd, Cr and Pb translocation from multinutrient fertilizer and to do speciation of As and Cr in this fertilizer and As in parts of plant in order to predict their phytoavailability. X-ray absorption near edge structure (XANES) was employed to speciate As and Cr directly on fertilizer solid sample. Arsenate (Asⱽ) and a solid solution of FeCrO₃ were the major species identified in the samples. The sprouts were hydroponically cultivated in water, fertilizer slurry and fertilizer extract media. Concentrations of As, Cd and Pb measured on leaves of maize sprouts ranged from 0.061 to 0.31 mg kg⁻¹, whereas Cr was not translocated to the aerial parts of sprouts. High performance liquid chromatographic with inductively coupled plasma mass spectrometry (HPLC-ICP-MS) analysis was used to determine As speciation in maize sprouts, as well as in the fertilizer extracts and slurries. Arsenate was the only species identified in the initial fertilizer extract and this information is in agreement with the XANES results. However, the reduction of arsenate to arsenite was observed in extracts and slurries collected after sprout growth, probably due to the action of exudates secreted by plant roots. Arsenite was the predominant species identified in sprouts, the high phosphate concentration in the medium may have contributed to reduce arsenate phytoavailability.

The high concentration of fluoride (F) in soils has become a rising concern for its toxicity to microbes, plants, animals and human health. In the present study, the spatial and vertical distribution, health risk assessment and anthropogenic sources of F in farmland soils in an industrial area dominated by phosphate chemical plants were studied. Concentrations of total fluoride (TF) and water soluble fluoride (WSF) in the surface soils decreased with distance within the range of 2500 m at the prevailing downwind of the industrial area. The soil TF and WSF concentrations in 0–40 cm profiles were higher than those in 40–100 cm layers in the industrial area. At the prevailing downwind of the industrial area within 700 m, the hazard quotient values of human exposure to surface soils were higher than 1, indicating that a potential risk may exist for human health in this area. The main exposure pathway for children and adults was oral ingestion and particulate inhalation, respectively. The source apportionment model of soil F was modified based on years’ historical data and experimental data. The results showed that the proportion of anthropogenic sources of soil F was dustfalls (69%) > irrigation water (23%) > air (5%) > chemical fertilizers (3%) in the industrial area. The high F concentration of dustfalls was mainly due to the phosphate rock, phosphogypsum, and surface soils with high F contents from the factories. In order to safeguard human health and alleviate hazards of F to surroundings, the control of pollutants emission from factories was a basic and vital step to reduce F in the soils in industrial areas.

The transfer of pollutants from chemical fertilizers through food webs within cropland is well documented; however, its impacts on the wild animals that forage on croplands but roost in other locations remain poorly understood. The potential for this cross-ecosystem ‘spillover’ of pollutants is greatest for bats, some of which exploit urban settlements as roosting niches but must travel long distances to reach croplands as foraging niches. Here, we used hairs from a colony of insectivorous bats, Chinese Noctule (Nyctalus plancyi), from an urban area in Southwest China to assess whether exposure to heavy metals/metalloids by the bats varied from 1975 to 2016. Historical changes occurred in hair cadmium (Cd) concentrations in adult females, which was exclusively explained by the regional fertilizer application intensity (FAI), even considering the potential impacts of Cd emissions in urban areas, as indicated by camphor trees (Cinnamomum camphora) near the bats' roosting niche, and the potential impacts of Cd in industrial wastewater, as documented in authorized databases. Therefore, the data from this bat colony, as urban dwellers, indicates Cd accumulation and cross-ecosystem transfer from rural croplands to an urban area.

A substantial amount of sulfate is often supplied in paddy fields with concomitant applications of chemical fertilizers and manure for rice growth. It is unclear how solubility and speciation of arsenic (As) are affected by the levels of soil sulfate and their relationship to soil redox status and sulfur (S) and iron (Fe) speciation in a short cycle of soil reducing (flooding) and oxidizing (drying) periods. The objective of this study was to investigate the solubility of As in relation to chemical speciation of As and S in different levels of soil sulfate through a time series of measurements during a 40-day reduction period (Eh < −130 mV) followed by a 32-day reoxidation period (Eh > 400 mV) using X-ray absorption fine structure (XAFS) spectroscopy. An excess of sulfate decreased extractable and dissolved As in the soil reducing period due to retardation of soil reduction process that decreased soluble As(III) in the soil solid phase. The As species at the end of soil reducing period were 38–41% As(V), 46–51% As(III), and 11–13% As2S3-like species, regardless of initial S treatments. In the following soil reoxidation, As2S3-like species were sensitive to oxidation and disappeared completely in the first 2 days when the Eh value increased rapidly above 160 mV. The addition of extra sulfate to the soil did not result in the formation of neither reduced S species nor As2S3-like species. About 50% of As(III) to the total As persisted over 32 days of soil reoxidation period (Eh > 400 mV), suggesting some mechanisms against oxidation of As(III) such as physical sequestration in soil microsites. This study demonstrates that the extra SO4 in paddy soils can help mitigate the dissolution of As in reduction and reoxidation periods.

Anaerobically digested and composted sewage sludge (CSS) has been suggested to be a slow-release fertilizer in forestry and an alternative to quick-release inorganic fertilizers. The effects of CSS with or without added carbohydrate on inorganic nitrogen availability and on soil animals were tested in two Norway spruce plantations. Half of the seedlings were individually fertilized with CSS, and the rest were left as controls. Solid sucrose was added to half of the fertilized and untreated seedlings. Soil samples were taken in the autumn in the first and the second year after the treatments. CSS increased soil NH4–N (2100%), the proportion of soil NO3–N, and the N concentration of spruce needles. CSS greatly reduced the abundances of enchytraeids, tardigrades and collembolans, but increased the proportion and abundance of bacterial-feeding nematodes irrespective of carbohydrate addition. A better stabilization method needs to be developed before CSS can be used as a forest fertilizer.

Nitrate pollution in oxygenated karst aquifers is common due to nitrification and anthropogenic inputs. However, the shift of nitrogen sources influenced by enhanced rural tourism activities and land use changes are not well understood. In this study, hydrochemistry and dual nitrate isotopes of water samples from a rural karst basin in Chongqing, southwestern China were employed to investigate the nitrate fate and its decadal change during the periods from 2007–2008 and 2017–2019. The results showed that δ¹⁵N–NO₃ and δ¹⁸O–NO₃ values at the groundwater basin resurgence averaged 9 ± 3.4‰ and 2.5 ± 3.4‰, respectively, with a mean NO₃⁻ concentration of 19.7 ± 5.4 mg/L in 2017–2019, clearly exceeding natural background levels. The dual isotope results suggested that nitrification occurred at the sampled sites. From 2007–2008 to 2017–2019, the mean δ¹⁵N–NO₃ values from the primary sink point and the resurgence of the underground river water samples increased from −0.2 ± 2.1 to 11.2 ± 4.8‰, 4.2 ± 0.9 to 9.0 ± 3.4‰, respectively. A Bayesian mixing model in R (MixSIAR) based on the isotopes revealed that soil organic nitrogen, and manure and sewage proportions for the groundwater increased by 34% and 23%, respectively, while chemical fertilizer and atmospheric precipitation proportions decreased by 32% and 25%, respectively. These decadal changes resulted from reforestation practices and enhanced rural tourism activities in the basin, which were evidenced by the change of land use patterns. The elevated nitrogen load from the rapid development of rural tourism is likely to increase this contamination in the near future if the infrastructure cannot meet the demands. The results from this study could contribute to minimizing environmental health risks in drinking water when rural tourism activities are increasing.

The rapid expansion of organic rice cultivation areas have been accompanied by increased application of organic fertilizers. The high prevalence of soil antibiotic resistance caused by organic fertilizer application poses a severe threat to the agricultural and soil ecosystems. To date, research efforts and understanding of the effects and mechanism of action of the various organic fertilizers on antibiotic resistance in paddy soils remain poorly investigated. Tetracycline resistance genes (TRGs, including tetB, tetC, tetL, tetZ, tetM, tetO, tetT, and tetX), class 1 integron-integrase gene (intI1) and bacterial communities were characterized using quantitative-PCR and Illumina MiSeq sequencing, in paddy soils exposed to inorganic fertilizer (NPK), animal-derived organic fertilizer (AOF, composted swine and/or chicken manure), plant-derived organic fertilizer (POF, rapeseed cake and/or astragalus) and commercial organic fertilizer (COF, composted of animal manure mix with crop residues) applications. Compared with NPK, AOF applications significantly increased the relative abundance of TRGs, which was predominantly expressed in the increase of the relative abundance of tetC, tetM, tetO, tetT, and tetX, while POF and COF had no significant effect on the relative abundance of TRGs. Principal coordinate analysis revealed that AOF and POF significantly altered bacterial communities in paddy soils relative to NPK, while COF had no significant change of bacterial communities. Variation partitioning analysis indicated that soil physicochemical properties were the decisive factors for the changes of TRGs in organic paddy fields. Furthermore, redundancy analysis and the Mantel test showed that TRG profiles in AOF applied paddy soils were strongly influenced by electrical conductivity (EC). Total nitrogen (TN) and organic matter (OM) affected the distribution of TRGs in COF and POF applied paddy soils through a different mechanism. This study provides insights into the impacts of different types of organic fertilizer on the profiles of TRGs in paddy soils.

Brazil is one of the major global poultry producers, and the organic waste generated by the chicken slaughterhouses can potentially be used as a biofertilizer in agriculture. This study was designed to test the hypothesis that continuous use of biofertilizer to the crops, substituting the use of mineral fertilizer promote C-offset for the soil and generate crop energy efficiency for the production system. Thus, the objectives of this study were to evaluate the effects of biofertilizer use alone or in combination with mineral fertilizer on soil organic carbon (SOC) stock, carbon dioxide (CO₂) mitigation, C-offset, crop energy efficiency and productivity, and alleviation of environmental pollution. The experiment was established in southern Brazil on a soil under 15 years of continuous no-till (NT). Experimental treatments were as follows: i) Control with no fertilizer application, ii) 100% use of industrial mineral fertilizer (Min-F); iii) 100% use of organic waste originated from poultry slaughterhouses and hereinafter designated biofertilizer (Bio-F), and iv) Mixed fertilizer equivalent to the use of 50% mineral fertilizer + 50% of biofertilizer (Mix-F). Effects of experimental treatments were assessed for the crop sequence based on bean (Phaseolus vulgaris), soybean (Glycine max) and corn (Zea mays) in the summer and wheat (Triticum aestivum) and black oat (Avena strigosaSchreb) in the winter composing two crops per year, as follow: bean/wheat-soybean/black oat-corn/wheat-soybean/black oat-corn/wheat-bean. The continuous use of Bio-F treatment significantly increased the index of crop energy efficiency. It was higher than that of control, and increased it by 25.4 Mg CO₂eq ha⁻¹ over that of Min-F treatment because of higher inputs of crop biomass-C into the system. Further, continuous use of Bio-F resulted in a significantly higher CO₂eq stock and offset than those for Min-F treatment. A positive relationship between the C-offset and the crop energy efficiency (R² = 0.71, p < 0.001) indicated that the increase of C-offset was associated with the increase of energy balance and the amount of SOC sequestered. The higher energy efficiency and C-offset by application of Bio-F indicated that the practice of crop bio fertilization with poultry slaughterhouse waste is a viable alternative for recycling and minimizing the environmental impacts.

Excessive nutrient balance is a very crucial issue for environmental hazards. The constant addition of high-amounts of nutrient sources in agricultural production generates negative environmental conditions in Korea and Japan yet to be resolved. Therefore, it is obligatory to comprehend the nutrient (nitrogen (N) and phosphorus (P)) balance that is assessed by the difference between nutrient input and output in the soil surface in Korea and Japan. Among 34 Economic Co-operation and Development (OECD) countries, Korea and Japan had the highest N and P balances and thus both countries are primarily responsible for severe environmental pollution via nutrient release. The cultivable land area in both countries has constantly decreased during 1990–2017 at approximately 20 and 15% in Korea and Japan, respectively. Even N and P use efficiency sharply decreased with increasing N and P balance in both targeted countries. Japanese P balance, Korean N and P balances were decreased after the mid-1990s whereas, Japanese N balance almost unchanged for the last 28 years. Unlike chemical fertilizer input, Korean manure input level significantly increased from 78 kg N ha⁻¹ in 1990 to 157 kg N ha⁻¹ in 2017. Japanese manure input level was higher than that of chemical fertilizer without any big change for the last 28 years. The lion share of high N and P balance in both countries could generate from manure inputs, therefore, the number of livestock and their produced debris need to be used with more cautious for the reduction of national N and P surpluses at a benchmark level. These findings ensure to make a more environment friendly policy that can further reduce nutrient balance as well as improve soil health.

The transport of trace metals in river–lake systems can potentially increase or decrease primary productivity in some basins and subsequently affect the carbon cycle of watersheds. In this study, we investigated a variety of trace metal concentrations and transport flux in the Poyang Lake basin during four seasons. Results show that the Gan River transports 78% of selenium (Se) and 42% of lead (Pb) into Poyang Lake each year, resulting in heavy metal pollution dominated by Pb and Se in 30%–75% of its water. Although toxic heavy metals, such as Pb, chromium (Cr), and copper (Cu), inhibit phytoplankton growth and decrease its gross primary productivity (GPP), excessive Se could effectually promote productivity. However, the negative effect of Pb on GPP is more significant than the positive effect of Se on GPP; hence, their interaction effectuates a decrease in total primary productivity. Additionally, under high nutrients level, the synergistic effect of heavy metals and nutrients will reduce GPP. Agricultural fertilizer is likely the source of both Pb, Cu, Se and N. Gan River contributes 35%–80% of the heavy metal inputs to Poyang Lake. It is therefore necessary to improve the ecological environment of phytoplankton and promote productivity in the Poyang Lake basin by reducing the application of agricultural chemical fertilizers to control pollution. Our results indicate that the role of certain, less studied trace elements (e.g., Pb, Cr, Cu, and Se) in regulating primary productivity of watershed ecosystems is more important than previously thought. This study also discusses potential impacting mechanisms associated with these metals on phytoplankton, whose biological functions need to be verified in future experiments.