The industrial production of insects for waste management or as a protein source is becoming vital to our society. Large volumes of manure are produced by concentrated animal facilities around the globe that must be managed, utilized, and disposed of properly. Flies offer a partial solution with their abilities to reduce these wastes and heavy metal pollutants. Meat and crop proteins are being supplemented by insect proteins for many feeds across the globe, yet science-based studies behind the mass-rearing of insects are still in their infancy. In the current study, the percent change in the composition of nutrients, heavy metals, and fiber, in dairy, poultry, and swine manure degraded by either black soldier fly (BSF) or house fly (HF) larvae was explored. Pre-digested and post-digested manure samples were collected from four independent studies that differed in production scale (number of larvae and feeding regimen): 1) BSF small-scale (100 larvae fed incrementally), 2) HF small-scale (100 larvae fed incrementally), 3) BSF large-scale (10,000 larvae fed a single time), and 4) HF large-scale (4,000 larvae fed a single time). Results indicate that nitrogen is a key nutrient impacted by larval digestion of manure by both species, regardless of scale. However, scale significantly impacted reductions of other nutrients, as did the type of manure in which the insects were reared. Ultimately, this study demonstrated that manure type and rearing scale impact the ability of BSF and HF larvae to reduce nutrients and heavy metals in manure, and thus insect management procedures need to be congruent with production emphases of the insects for waste management or protein products. Failure to take scale into consideration could lead to inaccurate assumptions related to industrialized efforts on this topic.

The house fly, Musca domestica L., is a cosmopolitan insect pest of public and animal health importance that serves as a mechanical vector of pathogens. Aimed at prospective resistance management to reduce environmental pollution, we characterized the inheritance pattern, realized heritability, fitness cost, cross resistance, stability and mechanism of clothianidin resistance in M. domestica that were collected from the poultry farm. By continuous selection with clothianidin for 11 generations, the clothianidin selected M. domestica strain (Clotha-SEL) developed a 3827-fold resistance compared to a susceptible strain. However, resistance to clothianidin was proved to be unstable when selection with clothianidin was removed for five generations (G₇ to G₁₂). Inheritance pattern analysis at G₈ of Clotha-SEL (RR = 897) revealed that resistance to clothianidin was polygenic, autosomal and incompletely dominant. Realized heritability (h²) for resistance value was 0.38 (at G₁₁) in the tested strain. Synergist bioassays showed that microsomal oxidases and esterases might not contribute significantly in resistance evolution. Fitness costs of clothianidin resistance were present, for example, reduction in growth potential of the Clotha-SEL strain in comparison to the untreated counterpart strain (UNSEL) was observed. No cross resistance to bifenthrin and fipronil and a very low cross-resistance to spinosad were observed. These insecticides could be alternated with clothianidin as an insecticide resistance management tool to sustain its efficacy for a longer time period. These results shall be utilized to devise a proactive resistance management strategy for use of clothianidin against M. domestica that will be helpful to alleviate the allied threats to environmental and human health.

Escherichia coli, as a global source of antimicrobial resistance, is a serious veterinary and public health concern. The transmission of pathogenic multidrug-resistant (MDR) E. coli within diarrheic calves and its correlation with Musca domestica and milk strains have been investigated. In total, 110, 80, and 26 E. coli strains were obtained from 70 rectal swabs from diarrheic calves, 60 milk samples and 20 M. domestica, respectively. Molecular pathotyping of E. coli revealed the presence of pathogenic E. coli with a higher percentage of shigatoxigenic strains within diarrheic calves and M. domestica at 46.4% and 34.6%, respectively. Phenotypic antimicrobial resistance revealed higher β-lactams resistance except for cefquinome that exhibited low resistance in M.domestica and milk strains at 30.8% and 30%, respectively. The extended-spectrum cephalosporin (ESC) resistant strains were detected within fecal, M. domestica, and milk strains at 69.1%, 73.1%, and 71.3%, respectively. All E. coli strains isolated from M. domestica exhibited MDR, while fecal and milk strains were harboring MDR at 99.1% and 85%, respectively. Molecular detection of resistant genes revealed the predominance of the blaTEM gene, while none of these strains harbored the blaOXA gene. The highest percentages for blaCTXM and blaCMYII genes were detected in M. domestica strains at 53.8% and 61.5%, respectively. Regarding colistin resistance, the mcr-1 gene was detected only in fecal and milk strains at 35.5% and 15%, respectively. A high frequency of phylogroup B2 was detected within fecal and M. domestica strains, while milk strains were mainly assigned to the B1 phylogroup. Pathogenic E. coli strains with the same phenotypic and genotypic antimicrobial resistance and phylogroups were identified for both diarrheic calves and M. domestica, suggesting that the possible role of M. domestica in disseminating pathogenic strains and antimicrobial resistance in dairy farms.

Medicinal and aromatic plants represent an outstanding source of green active ingredients for a broad range of real-world applications. In the present study, we investigated the insecticidal potential of the essential oils obtained from three medicinal and aromatic plants of economic importance in Algeria, Artemisia campestris, Pulicaria arabica, and Saccocalyx satureioides. Gas chromatography coupled with mass spectrometry (GC-MS) was used to study the essential oil chemical compositions. The three essential oils were tested against a mosquito vectoring filariasis and arboviruses, i.e., Culex quinquefasciatus, a fly pest acting also as pathogens vector, Musca domestica, and an agricultural moth pest, i.e., Spodoptera littoralis, using WHO and topical application methods, respectively. The essential oil from A. campestris, containing β-pinene (15.2%), α-pinene (11.2%), myrcene (10.3%), germacrene D (9.0%) (Z)-β-ocimene (8.1%) and γ-curcumene (6.4%), showed remarkable toxicity against C. quinquefasciatus (LC₅₀ of 45.8 mg L⁻¹) and moderate effects (LD₅₀ of 99.8 μg adult⁻¹) against M. domestica. Those from P. arabica and S. satureioides, containing epi-α-cadinol (23.9%), δ-cadinene (21.1%), α-cadinol (19.8%) and germacrene D-4-ol (8.4%), and thymol (25.6%), α-terpineol (24.6%), borneol (17.4%) and p-cymene (11.4%), respectively, were more active on S. littoralis showing LD₅₀ values of 68.9 and 61.2 μg larva⁻¹, respectively. Based on our results, the essential oil from A. campestris may be further considered a candidate ingredient for developing botanical larvicides.

Ocimum basilicum essential oil (EO) was evaluated for its biological effects on M. domestica. Characterization of O. basilicum EO revealed the presence of methyl chavicol (70.93%), linalool (9.34%), epi-α-cadinol (3.69 %), methyl eugenol (2.48%), γ-cadinene (1.67%), 1,8-cineole (1.30%) and (E)-β-ocimene (1.11%). The basil EO and its constituents methyl chavicol and linalool elicited a neuronal response in female adults of M. domestica. Adult female flies showed reduced preference to food source laced with basil EO and methyl chavicol. Substrates treated with EO and methyl chavicol at 0.25% resulted in an oviposition deterrence of over 80%. A large ovicidal effect was found for O. basilicum EO (EC₅₀ 9.74 mg/dm³) followed by methyl chavicol (EC₅₀ 10.67 mg/dm³) and linalool (EC₅₀ 13.57 mg/dm³). Adults exposed to EO (LD₅₀ 10.01 μg/adult) were more susceptible to contact toxicity than to methyl chavicol and linalool (LD₅₀ 13.62 μg/adult and LD₅₀ 43.12 μg/adult respectively). EO and its constituents methyl chavicol and linalool also induced the detoxifying enzymes Carboxyl esterase (Car E) and Glutathione S – transferases (GST).

The essential oil (EO) of Thymus serpyllum and thymol were evaluated for their insecticidal activity against the housefly (Musca domestica) larvae and pupae. Contact toxicity and fumigation bioassays were used. Chemical composition analysis of T. serpyllum EO by gas chromatographic mass spectrometry (GC-MS) revealed that thymol (41.6%), p-cymene (21.9%), and γ-terpinene (19.2%) were the major components. For larval assays, the LC₅₀ value of T. serpyllum EO was 0.4 μl/cm² for contact toxicity and 20.9 μl/l for fumigation toxicity. For thymol, the contact toxicity LC₅₀ value was 0.035 μl/cm² and the fumigation LC₅₀ value was 2.0 μl/l. For the pupal assay, T. serpyllum EO had a percentage inhibition rate (PIR) value of 100% for both contact toxicity (1.0 μl/cm²) and fumigation toxicity assay (25 μl/l), whereas thymol had a PIR of 100% for contact toxicity (0.1 μl/cm²) and fumigation assay (5 μl/l). This study shows that T. serpyllum EO and thymol are toxic to housefly larvae and pupae and have the potential for use in the population control of this species.

In this study, a mixed-level orthogonal array design was employed for the optimum conditions of breeding housefly larvae by food waste. The results showed that the effects of these factors on the weight of 50 larvae, larvae yield, and crude protein content were the culture substrate ratio > the breeding density > the feeding mode. The optimum conditions for the housefly larvae to convert food waste were as follows: culture substrates ratio 1:3, breeding density 10.0 g/kg, and all substance added on the first day. The optimum food waste mass reduction rate was 79.1–83.6%. The value of the essential amino acids (Eaa)/ the total amino acids (Taa) (45.1%) and E/the nonessential amino acid (Naa) values (0.83%) in the housefly larvae products met the Food and Agricultural Organization (FAO) requirements for feed protein. The crude fat content (30.1 ± 1.18%) was higher than of the housefly larvae after bioconversion of pig manure (22.0%) and the fish meal standard of China. The contents of total nutrients (N+P+K ≥ 5.5%) and heavy metals (Pb ≤ 0.40 mg/kg, Cr ≤ 1.50 mg/kg, Cd ≤ 0.40 mg/kg) in the residues of this study met the Chinese standard for organic fertilizer. Tilapia raised with the dried housefly larvae showed the best growth performance and nutrient concentrations in the experiment groups (p < 0.05). Moreover, the trace elements concentration in tilapia raised with the four kinds of feeds complied with the maximum levels of contaminants in foods in both China and WHO. These findings show that the housefly larvae products that converted food waste are suitable for use in the production of fish feed.

Echinophora spinosa (Apiaceae) is a psammophilous species growing along Mediterranean coastal sand dunes. This species secretes essential oils made up of monoterpenoids, phenylpropanoids, and polyacetylenes, which may represent effective, eco-friendly and safe active ingredients for the preparation of green insecticides. Based on this, in the present study, we selected a French accession of E. spinosa growing on the coastal dunes of Corsica, extracting the essential oils from aerial parts and roots by hydrodistillation. The essential oils were analyzed by GC-MS for chemical composition and screened for their insecticidal activity on three target insects, Culex quinquefasciatus, Spodoptera littoralis, and Musca domestica. The essential oil from roots was dominated by the phenylpropanoid myristicin and the monoterpene hydrocarbon terpinolene, with a minor contribution of (Z)-falcarinol. The oil from the aerial parts was characterized by monoterpenes, mainly p-cymene, a-phellandrene, and α-pinene. The root essential oil exhibited promising toxicity on the target insects, with special reference to C. quinquefasciatus larvae (LC₅₀ = 15.7 mg L⁻¹), while on M. domestica and S. littoralis, it showed LD₅₀ of 38.3 μg adult⁻¹ and 55.6 μg larva⁻¹, respectively. Based on our findings, E. spinosa can be viewed as a new potential crop to produce insecticidal essential oils in arid salty areas. However, synergistic and antagonistic effects of the major constituents of both oils tested here deserve future research. Further studies on the efficacy of stable formulations of the E. spinosa root essential oil, with special reference to micro- and nanoformulations, are ongoing.

The knockdown and adulticidal activities of individual Cymbopogon citratus and Eucalyptus globulus essential oils (EOs) and their combinations were evaluated against three medical insect pests (Aedes aegypti, Aedes albopictus, and Musca domestica) using a WHO susceptibility test. The knockdown and adulticidal activities against the three medical insect pests of combinations of C. citratus and E. globulus EOs were higher than those of individual EOs alone. Combinations of 7.5% C. citratus + 7.5% E. globulus EOs and 10% C. citratus + 10% E. globulus EOs exhibited the highest efficacy against females of the three species with 100% knockdown and mortality rates at 1 and 24 h after exposure, respectively. Their adulticidal activities were equivalent to that of 10% w/v cypermethrin. In contrast, 70% v/v ethyl alcohol negative control was not effective at all. The combinations of EOs showed a synergistic effect, i.e., their adulticidal activity was improved by 0.2 to 100%, with increased knockdown and mortality rates, compared to individual EOs. The highest synergistic effect on effective knockdown and adulticidal activities against females of the three species was achieved by a combination of 2.5% C. citratus + 2.5% E. globulus EOs, with 36.6 to 100% knockdown rate increase and 33.5 to 98.9% mortality rate increase. This study demonstrates that all tested combinations of C. citratus and E. globulus EOs were effective adulticidal agents against females of Ae. aegypti, Ae. albopictus, and M. domestica and have a high potential for development into a botanical insecticide for controlling populations of Aedes mosquitoes and houseflies.

In the attempt to exploit the potential of the monoecious fiber hemp cv. Futura 75 in new fields besides textile, cosmetics and food industry, its crop-residue given by leaves and inflorescences was subjected to hydrodistillation to obtain the essential oils. These are niche products representing an ideal candidate for the development of natural insecticides for the control and management of mosquito vectors, houseflies and moth pests. After GC-MS analysis highlighting a safe and legal chemical profile (THC in the range 0.004–0.012% dw), the leaf and inflorescence essential oils were investigated for the insecticidal potential against three insect targets: the larvae of Culex quinquefasciatus and Spodoptera littoralis and the adults of Musca domestica. The essential oil from inflorescences, showing (E)-caryophyllene (21.4%), myrcene (11.3%), cannabidiol (CBD, 11.1%), α-pinene (7.8%), terpinolene (7.6%), and α-humulene (7.1%) as the main components, was more effective than leaf oil against these insects, with LD₅₀ values of 65.8 μg/larva on S. littoralis, 122.1 μg/adult on M. domestica, and LC₅₀ of 124.5 μl/l on C. quinquefasciatus larvae. The hemp essential oil moderately inhibited the acetylcholinesterase (AChE), which is a target enzyme in pesticide science. Overall, these results shed light on the future application of fiber hemp crop-residue for the development of effective, eco-friendly and sustainable insecticides.