خيارات البحث
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Toxicological Effects of Simultaneous Exposure to Toluene and Noise on some Sexual and Stress Parameters in New Zealand White Rabbits
2023
Abouee-Mehrizi, Amirreza | Saed-Moucheshi, Shamseddin | Rasoulzadeh, Yahya | Mehdipour, Ahmad | Mohammadoghli-Reyhan, Fariba | Iraji, Zeynab | Alihemmati, Alireza | Shokoohi, Majid
Noise and toluene are among the numerous physical and chemical pollutants that can induce adverse effects on different body tissues and systems; nevertheless, most studies have only experimented the auditory changes induced by co-exposure to them. The present in-vivo study aimed to examine the endocrine effects of co-exposure to toluene and noise on the testes and adrenal glands. In this experimental study, 24 healthy male New Zealand White rabbits were used. The noise intensity was 100 dB (white noise) and the toluene concentration was 1000 ppm for two consecutive weeks. The luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone, cortisol and adrenocorticotropic hormone (ACTH) were measured using the enzyme-linked immunosorbent assay (ELISA) method. The hematoxylin and eosin stain method (H&E) was performed for the histopathological analysis. Comparing different parameters in different groups on post-exposure days was carried out using GEE (generalized estimating equations) method. The results indicated that noise and toluene increased cortisol, LH and FSH levels during different days after the exposure. Exposure to toluene and noise made vacuolization and reduction of primary spermatogonial cells in the testes. Moreover, lymphocyte infiltration, congestion, swelling and vacuolization were detected in adrenal glands through exposure to toluene and noise. Toluene and noise induced different destructive effects on the endocrine system. More studies are required to elucidate other endocrine changes induced by exposure to toluene and noise.
اظهر المزيد [+] اقل [-]Responses of Accessions of Zea Mays to Crude Oil Pollution Using Growth Indices and Enzyme Activities as Markers
2017
Njoku, K. L
The performance of every plant in an environment is an indicator of how the plant can withstand the various environmental conditions. This study investigated the toxicity of crude oil on the growth performance, chlorophyll contents, enzymatic activities and oxidative stress biomarkers of eight accessions of Zea mays. The growth enzyme (amylase and invertase) activities, as well as oxidative stress biomarkers (malondialdehyde, peroxidase, superoxide dismutase and catalase) in Z. mays were assayed using spectrophotometric method. The maize accessions were grown in the experimental pots containing crude oil treatments (2%, 4%, 6%, 8% and 10%) and harvested after 14 days of seedling emergence. The results showed that the percentage seedling emergence, leaf area, root length, stem girth and shoot length of each accession were significantly reduced (p
اظهر المزيد [+] اقل [-]Complex application of microbiological characteristics in bottom sediments and biochemical parameters of mussel Mytilus galloprovincialis (Lam.) for assessing the ecological state of marine coastal areas
2022
Skuratovskaya, Ekaterina | Doroshenko, Yuliya
Comparative analysis of microbiological indicators (heterotrophic and hydrocarbon-oxidizing bacteria) in bottom sediments and biochemical parameters (level of oxidized proteins (OP) and lipid peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities) in hepatopancreas of mussel Mytilus galloprovincialis (Lam.) from three Sevastopol bays - Laspi, Kazach’ya, Streletskaya (the Black Sea) was carried out. The results obtained allowed to identify certain differences between the studied areas and characterize their ecological state. The maximum abundance of heterotrophic and hydrocarbon-oxidizing bacteria was found in the most polluted Streletskaya Bay (95,000 and 250 cells/g respectively), the minimum - in the conventionally pure Laspi Bay (2,500 and 1.5 cells/g respectively). Parameters of prooxidant-antioxidant system (level of OP and LPO, SOD and CAT activities), as well as ALT activity in the hepatopancreas of mussels from Streletskaya Bay were found higher as compared to those in other tested areas. Most significant differences (more than twofold) in SOD activity, LPO content and basic ketone forms of OP levels were found between Laspi and Streletskaya bays. The results obtained indicate the severe pollution and less favorable living conditions for aquatic organisms in Streletskaya Bay, as well as demonstrate the high level of biochemical adaptation of mussels to complex environmental pollution. The studied parameters can be applied in the ecological monitoring of the coastal sea water areas.
اظهر المزيد [+] اقل [-]Effects of Cadmium and Dimethoate on Some Biological and Biochemical Indices in Freshwater Green Algae, Spirogyra sp.
2018
Banaee, M. | Taheri, S. | Hedayatzadeh, F.
The present study investigates the influence of an organophosphorus pesticide, namely Dimethoate, and cadmium on biomarkers of the green alga, Spirogyra sp., in a 14-day experiment. For so doing, it has exposed Spirogyra sp. to 0.0, 100, 200, and 400 mg L-1 of Dimethoate and/or 1 mg L-1 of cadmium chloride (CdCl2) to observe a reduction in chlorophyll a and b level in Spirogyra sp., exposed to 200 and 400 mg L-1 of Dimethoate as well as algae treated with cadmium alone or in combination with Dimethoate. Levels of malondialdehyde (MDA) and total antioxidant in cells, as well as the activity of ascorbate peroxidase (APX) soar in Spirogyra sp., exposed to Dimethoate and/or cadmium (alone or simultaneously). Also Spirogyra’sexposure to cadmium and/or Dimethoate significantly increases catalase (CAT) activity. However, levels of carotenoids in Spirogyra sp., treated with both cadmium and Dimethoate, decline significantly, with no significant change found in catalase activity of Spirogyra sp., exposed to 100 and 200 mg L-1 of Dimethoate, in comparison to the control group. However, CAT activity rises significantly in Spirogyra sp., treated with 400 mg L-1 of Dimethoate. Cadmium can cause cytotoxicity in 1 mg L-1 concentration of the green algae(Spirogyra sp.). On the whole, investigating the biological and biochemical markers in Spirogyra sp., exposed to different concentrations of Dimethoate, has revealed some concentration-dependent toxicity. Furthermore, Dimethoate can synergistically increase toxicity and bioavailability of cadmium in Spirogyra sp.
اظهر المزيد [+] اقل [-]Oxidative Stress Induction in Cassava Plant (Manihot Esculenta Crantz) Grown on Soil Contaminated with Diesel
2022
Osuntoki, Akinniyi | Olukanni, Olumide | Nwakile, Ogonna | Kabiru, Amusan
The induction of oxidative stress in plants grown on crude oil-contaminated soils was investigated using a diesel contaminated soils model. Twelve cassava stems were grown in four garden pots containing different amounts of diesel oil as contaminants: 150 ppm, 300 ppm, 600 ppm and control (0 ppm). The growth of the plants was monitored for 12 weeks, after which chlorophyll contents, total proteins, lipid peroxidation and activities of catalase, glutathione, and superoxide dismutase (antioxidant enzymes) were determined from the leaves. Significant decreases (p<0.05) were observed in the antioxidant enzymes (67-86%), total proteins (79%) and total chlorophyll content (67%) in the cassava grown on diesel contaminated soil (600 ppm) compared to the control. Consequently, there were significant increase (p<0.05) in the leaf ratio and malondialdehyde (a marker for lipid peroxidation) 0.1909 ± 04 and 1.77 ± 0.34, when compared to the control 0.1530 ± 08 sq.cm/g and 0.10±0.01 µmol/mg protein respectively. It was thus concluded that stunted growth of plants and their death in diesel or crude oil contaminated soil could be traced to oxidative stress.
اظهر المزيد [+] اقل [-]Integrated analysis of the quality of water bodies from the lower Paraná River basin with different productive uses by physicochemical and biological indicators
2020
Peluso, Julieta | Aronzon, Carolina Mariel | Ríos de Molina, María del Carmen | Rojas, Dante Emanuel | Cristos, Diego Sebastian | Pérez Coll, Cristina Silvia
The Paraná River basin is one of the most important in South America and is affected by human activities that take place on its margins. In particular, the De la Cruz stream flows through an industrial pole and the Arrecifes River goes mainly through agricultural fields. The aim of this study was to evaluate the water quality of the De la Cruz stream (S1) and the Arrecifes River (S2) by means of physicochemical parameters, including metals and pesticides concentrations. Since amphibians are good indicators of environmental quality, bioassays with Rhinella arenarum were carried on. For lethal and sublethal parameters, embryos and larvae were exposed to a dilution gradient of water samples and AMPHITOX Solution (AS) as negative control for 504 h. For the determination of oxidative stress biomarkers (Catalase -CAT-, Glutathione S-Transferase -GST-, Reduced Glutathione -GSH-, and lipid peroxidation -TBARS-), embryos and larvae were exposed to undiluted water samples and AS. For the determination of micronuclei, larvae at hind limb bud stage (S.28) were exposed to undiluted water samples, simultaneously with negative and positive controls (AS and cyclophosphamide 40 mg/L, respectively). Dissolved oxygen was low in both sites and the copper levels exceeded the Argentine limit for the protection of aquatic life. In embryos exposure, water sample from S1 caused lethal effects (504h-LC50 = 49 (28–71.6)%), increased TBARS levels, and GST and CAT activities. In larvae exposure, water sample from this site decreased CAT activity, while the water sample from S2 caused important lethal effects (504h-LC50 = 98.72 (60.60–302.52)%), low GSH levels and increased GST activity. Water samples from both sites induced higher micronuclei frequency than the negative control. This study alerts about the degradation of water quality of the studied sites including lethal and sublethal effects in R. arenarum that can jeopardize the native populations of this species. | Fil: Peluso, Julieta. Universidad Nacional de San Martín (UNSAM). Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. | Fil: Aronzon, Carolina Mariel. Universidad Nacional de San Martín (UNSAM). Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina. | Fil: Ríos de Molina, María del Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. | Fil: Rojas, Dante Emanuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Instituto de Química Biológica; Argentina. | Fil: Cristos, Diego Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Instituto de Química Biológica; Argentina. | Fil: Pérez Coll, Cristina Silvia. Universidad Nacional de San Martín (UNSAM). Instituto de Investigación e Ingeniería Ambiental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.
اظهر المزيد [+] اقل [-]Ecotoxicity of single and mixture of perfluoroalkyl substances (PFOS and PFOA) in soils to the earthworm Aporrectodea caliginosa
2023
Delor, L. | Louzon, M. | Pelosi, Céline | Michel, Eric | Maillet, G. | Carronnier, H. | Environnement Méditerranéen et Modélisation des Agro-Hydrosystèmes (EMMAH) ; Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
International audience | Per and polyfluoroalkyl substances (PFAS) are persistent compounds that are massively used in industry, consumer goods and fire-fighting foams. Soil contamination by PFAS is a major environmental concern, and there is a lack of knowledge on both their ecotoxicological mechanisms and the concentrations that induce adverse effects especially to non-target organisms, particularly in the case of PFAS mixtures. This study contributes to filling these gaps by assessing and modelling the effects of PFAS (in single and in mixtures for PFOS and PFOA at different environmental doses) on juvenile endogeic earthworms of a common species in European soils (Aporrectodea caliginosa) at different levels of biological organization (sub-individual and individual). The results showed for the first time combined strong ecotoxicological effects of PFAS on earthworm survival, integumental integrity, growth, sexual maturity and on genomic stability notably with the induction of DNA breaks associated with no abnormal oxidative DNA-lesion levels. Our results demonstrated significant effects at 0.3 mg kg − 1 and additive effects in case of mixtures. ☆ This paper has been recommended for acceptance by Jiayin Dai.
اظهر المزيد [+] اقل [-]Metabolic, cellular and defense responses to single and co-exposure to carbamazepine and methylmercury in Dreissena polymorpha
2022
Baratange, Clément | Paris-Palacios, Séverine | Bonnard, Isabelle | Delahaut, Laurence | Dominique, Grandjean | Wortham, Laurence | Sayen, Stéphanie | Gallorini, Andrea | Michel, Jean | Renault, D | Breider, Florian | Loizeau, Jean-Luc | Cosio, Claudia | Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO) ; Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH) ; Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet ; Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS) | School of Architecture, Civil and Environmental Engineering (ENAC) ; Ecole Polytechnique Fédérale de Lausanne (EPFL) | Pathologies Pulmonaires et Plasticité Cellulaire - UMR-S 1250 (P3CELL) ; Université de Reims Champagne-Ardenne (URCA)-Institut National de la Santé et de la Recherche Médicale (INSERM) | Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR) ; Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie - CNRS Chimie (INC-CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé) ; Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-SFR Condorcet ; Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS) | Université de Genève = University of Geneva (UNIGE) | Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO) ; Université de Rennes (UR)-Institut Ecologie et Environnement - CNRS Ecologie et Environnement (INEE-CNRS) ; Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR) ; Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS)
International audience | Carbamazepine (CBZ) and Hg are widespread and persistent micropollutants in aquatic environments. Both pollutants are known to trigger similar toxicity mechanisms, e.g. reactive oxygen species (ROS) production. Here, their effects were assessed in the zebra mussel Dreissena polymorpha, frequently used as a freshwater model in ecotoxicology and biomonitoring. Single and co-exposures to CBZ (3.9 µg•L-1) and MeHg (280 ng•L-1) were performed for 1 and 7 days. Metabolomics analyses evidenced that the co-exposure was the most disturbing after 7 days, reducing the amount of 25 metabolites involved in protein synthesis, energy metabolism, antioxidant response and osmoregulation, and significantly altering cells and organelles' structure supporting a reduction of functions of gills and digestive glands. CBZ alone after 7 days decreased the amount of α-aminobutyric acid and had a moderate effect on the structure of mitochondria in digestive glands. MeHg alone had no effect on mussels' metabolome, but caused a significant alteration of cells and organelles' structure in gills and digestive glands. Single exposures and the co-exposure increased antioxidant responses vs control in gills and digestive glands, without resulting in lipid peroxidation, suggesting an increased ROS production caused by both pollutants. Data globally supported that a higher number of hyperactive cells compensated cellular alterations in the digestive gland of mussels exposed to CBZ or MeHg alone, while CBZ+MeHg co-exposure overwhelmed this compensation after 7 days. Those effects were unpredictable based on cellular responses to CBZ and MeHg alone, highlighting the need to consider molecular toxicity pathways for a better anticipation of effects of pollutants in biota in complex environmental conditions.
اظهر المزيد [+] اقل [-]Ecotoxicity of single and mixture of perfluoroalkyl substances (PFOS and PFOA) in soils to the earthworm Aporrectodea caliginosa
2023
Delor, L. | Louzon, M. | Pelosi, Céline | Michel, Eric | Maillet, G. | Carronnier, H. | Valgo | Envisol | Environnement Méditerranéen et Modélisation des Agro-Hydrosystèmes (EMMAH) ; Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | TOXEM
International audience | Per and polyfluoroalkyl substances (PFAS) are persistent compounds that are massively used in industry, consumer goods and fire-fighting foams. Soil contamination by PFAS is a major environmental concern, and there is a lack of knowledge on both their ecotoxicological mechanisms and the concentrations that induce adverse effects especially to non-target organisms, particularly in the case of PFAS mixtures. This study contributes to filling these gaps by assessing and modelling the effects of PFAS (in single and in mixtures for PFOS and PFOA at different environmental doses) on juvenile endogeic earthworms of a common species in European soils (Aporrectodea caliginosa) at different levels of biological organization (sub-individual and individual). The results showed for the first time combined strong ecotoxicological effects of PFAS on earthworm survival, integumental integrity, growth, sexual maturity and on genomic stability notably with the induction of DNA breaks associated with no abnormal oxidative DNA-lesion levels. Our results demonstrated significant effects at 0.3 mg kg − 1 and additive effects in case of mixtures. ☆ This paper has been recommended for acceptance by Jiayin Dai.
اظهر المزيد [+] اقل [-]Earthworm tolerance to residual agricultural pesticide contamination: field and experimental assessment of detoxification capabilities
2014
Givaudan, Nicolas | Binet, Françoise | Le Bot, Barbara | Wiegand, Claudia | Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO) ; Université de Rennes (UR)-Institut Ecologie et Environnement (INEE) ; Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR) ; Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS) | Institut de recherche en santé, environnement et travail (Irset) ; Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) | École des Hautes Études en Santé Publique [EHESP] (EHESP) | European University of Brittany via the International Chair of Excellence in Agronomy and Environment granted to C. Wiegand and to ECOBIO laboratory, and by the Institute français du Danemark. It is also part of the LIA "Environmental Toxicology and Stress Ecology" sustained by the CNRS-INEE, the University of South Danemark and the University of Rennes1.
International audience | This study investigates if acclimatization to residual pesticide contamination in agricultural soils is reflected in detoxification, antioxidant enzyme activities and energy budget of earthworms. Five fields within a joint agricultural area exhibited different chemical and farming histories from conventional cultivation to organic pasture. Soil multiresidual pesticide analysis revealed up to 9 molecules including atrazine up to 2.4 ng g(-1) dry soil. Exposure history of endogeic Aporrectodea caliginosa and Allolobophora chlorotica modified their responses to pesticides. In the field, activities of soluble glutathione-S-transferases (sGST) and catalase increased with soil pesticide contamination in A. caliginosa. Pesticide stress was reflected in depletion of energy reserves in A. chlorotica. Acute exposure of pre-adapted and naïve A. caliginosa to pesticides (fungicide Opus(®), 0.1 μg active ingredient epoxiconazole g(-1) dry soil, RoundUp Flash(®), 2.5 μg active ingredient glyphosate g(-1) dry soil, and their mixture), revealed that environmental pre-exposure accelerated activation of the detoxification enzyme sGST towards epoxiconazole.
اظهر المزيد [+] اقل [-]