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Adaptive resilience of roadside trees to vehicular emissions via leaf enzymatic, physiological, and anatomical trait modulations
2022
Unplanned urbanization and heavy automobile use by the rapidly growing population contribute to a variety of environmental issues. Roadside plants can mitigate air pollution by modifying their enzymatic activity, physiological and anatomical traits. Plant enzymes, physiological and anatomical traits play an important role in adaptation and mitigation mechanisms against vehicular emissions. There is a significant gap in understanding of how plant enzymes and anatomical traits respond or how they participate in modulating the effect of vehicular emissions/air pollution. Modulation of leaf anatomical traits is also useful in regulating plant physiological behavior. Hence, the present study was conducted to evaluate the effects of vehicular pollution on the enzymatic activity, physiological, and anatomical traits of plant species that grow in forests (S1) and alongside roads (S2-1 km away from the S1 site) during different seasons. The present study examines four commonly found roadside tree species i.e. Grevillea robusta, Cassia fistula, Quercus leucotrichophora and Cornus oblonga. The study found that the activities of catalase and phenylalanine ammonium enzymes were higher in G. robusta species of roadside than control site (S1). Non-enzymatic antioxidants such as flavonoid and phenol were also found in higher concentrations in roadside tree species during the summer season. However, the measured values of physiological traits were higher in Q. leucotrichophora tree species of S1 during the summer season. When compared to the other species along the roadside, Q. leucotrichophora had the highest number of stomata and epidermal cells during the summer season. Hence, we found that tree species grown along the roadside adapted towards vehicular emissions by modulating their enzymatic, physiological, and anatomical traits to mitigate the effect of air pollution.
اظهر المزيد [+] اقل [-]Trophic transfer of persistent toxic substances through a coastal food web in Ulsan Bay, South Korea: Application of compound-specific isotope analysis of nitrogen in amino acids
2020
An, Yoonyoung | Hong, Seongjin | Kim, Youngnam | Kim, Mungi | Choi, Bohyung | Won, Eun-Ji | Shin, Kyung-Hoon
Trophic magnification factor (TMF) of persistent toxic substances (PTSs: Hg, PCBs, PAHs, and styrene oligomers (SOs)) in a coastal food web (12 fish and four invertebrates) was determined in Ulsan Bay, South Korea. The nitrogen stable isotope ratios (δ¹⁵N) of amino acids [δ¹⁵NGₗᵤ₋Pₕₑ based on glutamic acid (δ¹⁵NGₗᵤ) and phenylalanine (δ¹⁵NPₕₑ)] were used to estimate the trophic position (TPGₗᵤ₋Pₕₑ) of organisms. The TPGₗᵤ₋Pₕₑ of organisms ranged from 1.64 to 3.69, which was lower than TP estimated by δ¹⁵N of bulk particulate organic matter (TPBᵤₗₖ: 2.46–4.21). Mercury and CB 138, 153, 187, and 180 were biomagnified through the whole food web (TMF > 1), while other PTSs, such as PAHs and SOs were not (biodilution of SOs firstly reported). In particular, the trophic transfer of PTSs was pronounced in the resident fish (e.g., rock bream, sea perch, Korean rockfish). Of note, CB 99, 101, 118, and 183 were additionally found to be biomagnifying PTSs in these species. Thus, fish residency appears to represent an important factor in determining the TMF of PTSs in the coastal environment. Overall, δ¹⁵NGₗᵤ₋Pₕₑ provided accurate TPs of organisms and could be applied to determine the trophic transfer of PTSs in coastal food webs.
اظهر المزيد [+] اقل [-]Metabolic profiling study on potential toxicity in male mice treated with Dechlorane 602 using UHPLC-ESI-IT-TOF-MS
2019
Tao, Wuqun | Tian, Jijing | Xu, Tuan | Xu, Li | Xie, Heidi Qunhui | Zhou, Zhiguang | Guo, Zhiling | Fu, Hualing | Yin, Xuejiao | Chen, Yangsheng | Xu, Haiming | Zhang, Songyan | Zhang, Wanglong | Ma, Chao | Ji, Feng | Yang, Jun | Zhao, Bin
Dechlorane 602 (Dec 602), a chlorinated flame retardant, has been widely detected in different environmental matrices and biota. However, toxicity data for Dec 602 seldom have been reported. A metabolomics study based on ultra-high performance liquid chromatography coupled with ion trap time-of-flight mass spectrometry was employed to study the urine and sera metabolic profiles of mice administered with Dec 602 (0, 0.001, 0.1, and 10 mg/kg body weight per day) for 7 days. A significant difference in metabolic profiling was observed between the Dec 602 treated group and the control group by multivariate analysis, which directly reflected the metabolic perturbations caused by Dec 602. The metabolomics analyses of urine from Dec 602-exposed animals exhibited an increase in the levels of thymidine and tryptophan as well as a decrease in the levels of tyrosine, 12,13-dihydroxy-9Z-octadecenoic acid, 2-hydroxyhexadecanoic acid and cuminaldehyde. The metabolomics analyses of sera showed a decrease in the levels of kynurenic acid, daidzein, adenosine, xanthurenic acid and hypoxanthine from Dec 602-exposed animals. These findings indicated Dec 602 induced disturbance in phenylalanine, tyrosine and tryptophan biosynthesis, tryptophan metabolism, tyrosine metabolism, pyrimidine metabolism, purine metabolism, ubiquinone and other terpenoid-quinone biosynthesis; phenylalanine metabolism and aminoacyl-tRNA biosynthesis. Significant alterations of immune and neurotransmitter-related metabolites (tyrosine, tryptophan, kynurenic acid, and xanthurenic acid) suggest that the toxic effects of Dec 602 may contribute to its interactions with the immune and neuronal systems. This study demonstrated that the UHPLC-ESI-IT-TOF-MS-based metabolomic approach can obtain more specific insights into the potential toxic effects of Dec 602 at molecular level.
اظهر المزيد [+] اقل [-]The response of the algae Fucus virsoides (Fucales, Ochrophyta) to Roundup® solution exposure: A metabolomics approach
2019
Felline, S. | Del Coco, L. | Kaleb, S. | Guarnieri, G. | Fraschetti, S. | Terlizzi, A. | Fanizzi, F.P. | Falace, A.
Glyphosate, as a broad-spectrum herbicide, is frequently detected in water and several studies have investigated its effects on several freshwater aquatic organisms. Yet, only few investigations have been performed on marine macroalgae. Here, we studied both the metabolomics responses and the effect on primary production in the endemic brown algae Fucus virsoides exposed to different concentration (0, 0.5, 1.5 and 2.5 mg L⁻¹) of a commercial glyphosate-based herbicide, namely Roundup®. Our results show that Roundup® significantly reduced quantum yield of photosynthesis (Fᵥ/Fₘ) and caused alteration in the metabolomic profiles of exposed thalli compared to controls. Together with the decrease in the aromatic amino acids (phenylalanine and tyrosine), an increase in shikimate content was detected. The branched-amino acids differently varied according to levels of herbicide exposure, as well as observed for the content of choline, formate, glucose, malonate and fumarate. Our results suggest that marine primary producers could be largely affected by the agricultural land use, this asking for further studies addressing the ecosystem-level effects of glyphosate-based herbicides in coastal waters.
اظهر المزيد [+] اقل [-]Salinity mediated cross-tolerance of arsenic toxicity in the halophyte Salvadora persica L. through metabolomic dynamics and regulation of stomatal movement and photosynthesis
2022
Patel, Monika | Parida, Asish Kumar
Arsenic (As) is a highly toxic metalloid adversely affecting the environment, human health, and crop productivity. The present study assessed the synergistic effects of salinity and As on photosynthetic attributes, stomatal regulations, and metabolomics responses of the xero-halophyte Salvadora persica to decipher the As-salinity cross-tolerance mechanisms and to identify the potential metabolites/metabolic pathways involved in cross-tolerance of As with salinity. Salinity and As stress-induced significant stomatal closure in S. persica suggests an adaptive response to decrease water loss through transpiration. NaCl supplementation improved the net photosynthetic rate (by +39%), stomatal conductance (by +190%), water use efficiency (by +55%), photochemical quenching (by +37%), and electron transfer rate (54%) under As stress as compared to solitary As treatment. Our results imply that both stomatal and non-stomatal factors account for a reduction in photosynthesis under high salinity and As stress conditions. A total of 64 metabolites were identified in S. persica under salinity and/or As stress, and up-regulation of various metabolites support early As-salinity stress tolerance in S. persica by improving antioxidative defense and ROS detoxification. The primary metabolites such as polyphenols (caffeic acid, catechin, gallic acid, coumaric acid, rosmarinic acid, and cinnamic acid), amino acids (glutamic acid, cysteine, glycine, lysine, phenylalanine, and tyrosine), citrate cycle intermediates (malic acid, oxalic acid, and α-ketoglutaric acid), and most of the phytohormones accumulated at higher levels under combined treatment of As + NaCl compared to solitary treatment of As. Moreover, exogenous salinity increased glutamate, glycine, and cysteine, which may induce higher synthesis of GSH-PCs in S. persica. The metabolic pathways that were significantly affected in response to salinity and/or As include inositol phosphate metabolism, citrate cycle, glyoxylate and dicarboxylate metabolism, amino acid metabolism, and glutathione metabolism. Our findings indicate that inflections of various metabolites and metabolic pathways facilitate S. persica to withstand and grow optimally even under high salinity and As conditions. Moreover, the addition of salt enhanced the arsenic tolerance proficiency of this halophyte.
اظهر المزيد [+] اقل [-]Influence of fuel oil on Platymonas helgolandica: An acute toxicity evaluation to amino acids
2021
Li, Na | Liu, Yu | Liang, Zhengyu | Lou, Yadi | Liu, Yuxin | Zhao, Xinda | Wang, Guoguang
It is highly likely that the toxicity of water accommodated fractions (WAF) will influence marine microalgae, and consequently lead to potential risk for the marine ecological environment. However, it was often neglected whether WAF can influence the transformation of relative compounds in organisms. The metabolism of amino acids (AAs) can be used to track physiological changes in microalgae because amino acids are the basis of proteins and enzymes. In this study, using marine Chlorophyta Platymonas helgolandica as the test organism, the effects of different concentrations of WAF on AA compositions and stable carbon isotope ratios (δ¹³C) of individual AAs of Platymonas helgolandica were investigated. The results showed that the WAF of #180 fuel oil had an obvious suppressing effect on the growth and chlorophyll a content of microalgae. The growth inhibitory rate at 96 h was 80.66% at a WAF concentration of 0.50 mg L⁻¹ compared with the control. Furthermore, seven among the 16 AAs, including alanine, cysteine, proline, aspartic acid, lysine, histidine and tyrosine, had relatively high abundance. Under the glycolysis pathway, the cysteine abundance was higher than control, meaning that the biosynthesized pathway of alanine through cysteine as a precursor could be damaged. Phosphoenolpyruvate (PEP) was an important synthesis precursor of alanine (leucine) and aromatic AA family (Phenylalanine and tyrosine), and played an important role in δ¹³CAAₛ fractionation under the WAF stress. Under the TCA pathway, to protect cell metabolism activities under WAF stress, the δ¹³C value of threonine and proline abundance in microalgae with the increase in WAF stress. Therefore, δ¹³CAAₛ fractionation can be used as a novel method for toxicity evaluation of WAF on future.
اظهر المزيد [+] اقل [-]Rice grains alleviate cadmium toxicity by expending glutamate and increasing manganese in the cadmium contaminated farmland
2020
Yuan, Kai | Wang, Changrong | Zhang, Changbo | Huang, Yongchun | Wang, Peipei | Liu, Zhongqi
The accumulation of cadmium (Cd) in rice grains is closely associated with the content of mineral nutrients and amino acid metabolism, but the causal link among them is unclear. Profiles of amino acids (AAs) and quantities of essential nutrients in grains from early and late rice cultivars grown at four sites with different Cd levels were analyzed in the present study. Hazard quotients (HQs) for consumers by intake of rice from late cultivars were much higher than that from early cultivars at sites with soil Cd content of 0.25, 0.61 and 0.84 mg kg⁻¹. Cadmium accumulation in grains resulted in a sharp reduction of total essential AAs and non-essential AAs in both early and late rice cultivars. High-Cd-accumulating (HCA) cultivars had significantly higher level of glutamate (Glu) than low-Cd-accumulating (LCA) cultivars when rice Cd content was less than 0.20 mg kg⁻¹. However, Glu level in grains dramatically declined with the accumulation of Cd, which subsequently leaded to the reduction of other AAs. Cadmium content was well predicted by five amino acids (i.e., Glu, Alanine, Phenylalanine, Glycine and Threonine) or four essential elements (Ca, Fe, Mn and Zn) when rice Cd was less than 0.80 mg kg⁻¹. Amino acids played more important roles than nutrients in Cd accumulation. When Cd content was in the range of 0.40–1.16 mg kg⁻¹, the Mn content in rice increased significantly with the increase of Cd content, while the Glu content dropped down synchronously. Remarkably, the ratio between Mn and Glu displayed the highest direct path coefficient on Cd accumulation than any single cation or amino acid. These results indicate that high capacity in synthesizing Glu and concentrating Mn is the determinant factor for Cd accumulation in rice grains, and abundant Glu in aleurone layer may alleviate Cd toxicity by forming Glu-Cd complex.
اظهر المزيد [+] اقل [-]Determination of precise nitrogen stable isotopic baselines from heterotrophic organism in coastal ocean environments using compound specific isotope analysis of amino acids
2021
Choi, Bohyung | Shin, Kyung-Hoon
We sampled mussels (Mytilus edulis) along the coast of the Korean peninsula, and determined their trophic position (TP) using the nitrogen isotope ratio of amino acids (TPgₗᵤ/ₚₕₑ). The TPgₗᵤ/ₚₕₑ of mussels (2.0 to 2.5) is higher than TP of herbivores, suggesting that TP normalization is required to remove the trophic enrichment effect on basal nitrogen isotope ratio. We found similar trends in spatial variation between nitrogen isotopic baseline based on nitrogen isotope ratio of bulk tissue (δ¹⁵Nbₐₛₑ₋bᵤₗₖ) and that of phenylalanine (δ¹⁵Nbₐₛₑ₋ₚₕₑ) in mussels, reflecting different nitrogen sources among sampling sites. The present study suggests that δ¹⁵Nbₐₛₑ₋bᵤₗₖ and δ¹⁵Nbₐₛₑ₋ₚₕₑ are powerful tools to trace nitrogen isotopic baselines from primary consumers as well as higher TP organisms.
اظهر المزيد [+] اقل [-]Insight into the differences in the toxicity mechanisms of dinotefuran enantiomers in zebrafish by UPLC-Q/TOF–MS
2022
Zhou, Xia | Yang, Ya | Ming, Renyue | Chen, Hong | Hu, Deyu | Lu, Ping
Dinotefuran is a chiral insecticide widely used to control Nilaparvata lugens in agriculture. However, little is known about the toxic effects of dinotefuran enantiomers on aquatic organisms. In this study, zebrafish were exposed to 1.00 and 10.00 mg/L dinotefuran enantiomers for 96 h, after which multivariate pattern recognition, metabolite identification, and pathway analysis were performed. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were then conducted to reveal the metabolic perturbations caused by dinotefuran enantiomers. Metabolic pathway analysis revealed the perturbation of five main pathways, including phenylalanine, tyrosine and tryptophan biosynthesis; phenylalanine metabolism; retinol metabolism; arginine and proline metabolism; and glycerophospholipid metabolism. These disturbed metabolic pathways were strongly correlated with energy, amino acid metabolism, and lipid metabolism. Pathway analysis also indicated that the metabolic pathway changes induced by the same level of R and S-dinotefuran were enantioselective. Our research may provide better insight into the risk of chiral dinotefuran in aquatic organisms in the environment.
اظهر المزيد [+] اقل [-]Evaluation of 2,4-dichlorophenol exposure of Japanese medaka, Oryzias latipes, using a metabolomics approach
2017
Kokushi, Emiko | Shintoyo, Aoi | Koyama, Jiro | Uno, Seiichi
In this study, the metabolic effects of waterborne exposure of medaka (Oryzias latipes) to nominal concentrations of 20 (L group) and 2000 μg/L (H group) 2,4-dichlorophenol (DCP) were examined using a gas chromatography/mass spectroscopy (GC/MS) metabolomics approach. A principal component analysis (PCA) separated the L, H, and control groups along PC1 to explain the toxic effects of DCP at 24 h of exposure. Furthermore, the L and H groups were separated along PC1 at 96 h on the PCA score plots. These results suggest that the effects of DCP depended on exposure concentration and time. Changes in tricarboxylic cycle metabolites suggested that fish exposed to 2,4-DCP require more energy to metabolize and eliminate DCP, particularly at 96 h of exposure. A time-dependent response in the fish exposed to DCP was observed in the GC/MS data, suggesting that the higher DCP concentration had greater effects at 24 h than those observed in response to the lower concentration. In addition, several essential amino acids (arginine, histidine, lysine, isoleucine, leucine, methionine, phenylalanine, threonine, tryptophan, and valine) decreased after DCP exposure in the H group, and starvation condition and high concentration exposure of DCP could consume excess energy from amino acids.
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