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النتائج 1 - 8 من 8
Differences in EDTA-assisted metal phytoextraction between metallicolous and non-metallicolous accessions of Rumex acetosa L
2010
Barrutia, Oihana | Garbisu, Carlos | Hernández-Allica, Javier | García-Plazaola, José Ignacio | Becerril, José María
Two common sorrel (Rumex acetosa) accessions, one from a Zn–Pb contaminated site (CS accession) and the other from an uncontaminated site (UCS accession), were hydroponically exposed to a mixture of heavy metals (Pb2+ + Zn2+ + Cd2+) with and without EDTA at an equimolar rate. The metallicolous CS accession showed a higher tolerance to metal treatment in the absence of the chelating agent, whereas the UCS accession was especially tolerant to EDTA treatment alone. Combination of metal and EDTA treatment resulted in a higher Pb accumulation in shoots of both accessions although plants hardly showed phytotoxic symptoms. Cd and Zn uptake was not augmented by EDTA addition to the polymetallic medium. Chelant-assisted Pb accumulation was 70% higher in the CS accession than in the UCS accession, despite the fact that the former accession evapotranspired less water than the UCS accession. These results support the existence of a non-selective apoplastic transport of metal chelates by R. acetosa roots, not related to transpiration stream.
اظهر المزيد [+] اقل [-]Endophytic bacteria improve phytoremediation of Ni and TCE co-contamination
2010
Weyens, Nele | Croes, Sarah | Dupae, Joke | Newman, Lee | Lelie, Daniel van der | Carleer, Robert | Vangronsveld, Jaco
The aim of this work was to investigate if engineered endophytes can improve phytoremediation of co-contaminations by organic pollutants and toxic metals. As a model system, yellow lupine was inoculated with the endophyte Burkholderia cepacia VM1468 possessing (a) the pTOM-Bu61 plasmid, coding for constitutive trichloroethylene (TCE) degradation, and (b) the ncc-nre Ni resistance/sequestration system. Plants were exposed to Ni and TCE and (a) Ni and TCE phytotoxicity, (b) TCE degradation and evapotranspiration, and (c) Ni concentrations in the roots and shoots were determined. Inoculation with B. cepacia VM1468 resulted in decreased Ni and TCE phytotoxicity, as measured by 30% increased root biomass and up to 50% decreased activities of enzymes involved in anti-oxidative defence in the roots. In addition, TCE evapotranspiration showed a decreasing trend and a 5 times higher Ni uptake was observed after inoculation.
اظهر المزيد [+] اقل [-]Dynamics of PCB removal and detoxification in historically contaminated soils amended with activated carbon
2010
Vasilyeva, Galina K. | Strijakova, Elena R. | Nikolaeva, Svetlana N. | Lebedev, Albert T. | Shea, Patrick J.
Activated carbon (AC) can help overcome toxicity of pollutants to microbes and facilitate soil bioremediation. We used this approach to treat a Histosol and an Alluvial soil historically contaminated with PCB (4190 and 1585 mg kg−1, respectively; primarily tri-, tetra- and pentachlorinated congeners). Results confirmed PCB persistence; reductions in PCB extractable from control and AC-amended soils were mostly due to a decrease in tri- and to some extent tetrachlorinated congeners as well as formation of a bound fraction. Mechanisms of PCB binding by soil and AC were different. In addition to microbial degradation of less chlorinated congeners, we postulate AC catalyzed dechlorination of higher chlorinated congeners. A large decrease in bioavailable PCB in AC-amended soils was demonstrated by greater clover germination and biomass. Phytotoxicity was low in treated soils but remained high in untreated soils for the duration of a 39-month experiment. These observations indicate the utility of AC for remediation of soils historically contaminated with PCB. Activated carbon promotes remediation of soils historically contaminated with PCB.
اظهر المزيد [+] اقل [-]Phytotoxicity of atrazine, isoproturon, and diuron to submersed macrophytes in outdoor mesocosms
2010
Knauert, Stefanie | Singer, Heinz | Hollender, Juliane | Knauer, Katja
The submersed macrophytes Elodea canadensis, Myriophyllum spicatum and Potamogeton lucens were constantly exposed over a five-week period to environmentally relevant concentrations of atrazine, isoproturon, diuron, and their mixture in outdoor mesocosms. Effects were evaluated investigating photosynthetic efficiency (PE) of the three macrophytes and growth of M. spicatum and E. canadensis. Adverse effects on PE were observed on days 2 and 5 after application. M. spicatum was found to be the more sensitive macrophyte. E. canadensis and P. lucens were less sensitive to atrazine, diuron and the mixture and insensitive to isoproturon. PE of M. spicatum was similarly affected by the single herbicides and the mixture demonstrating concentration addition. Growth of E. canadensis and M. spicatum was not reduced indicating that herbicide exposure did not impair plant development. Although PE measurements turned out to be a sensitive method to monitor PSII herbicides, plant growth remains the more relevant ecological endpoint in risk assessment. Short-term effects on photosynthesis did not result in growth reduction of submerse macrophytes exposed to PSII inhibitors.
اظهر المزيد [+] اقل [-]Pd-nanoparticles cause increased toxicity to kiwifruit pollen compared to soluble Pd(II)
2010
Speranza, Anna | Leopold, Kerstin | Maier, Marina | Taddei, Anna Rita | Scoccianti, Valeria
In the present study, endpoints including in vitro pollen performance (i.e., germination and tube growth) and lethality were used as assessments of nanotoxicity. Pollen was treated with 5–10 nm-sized Pd particles, similar to those released into the environment by catalytic car exhaust converters. Results showed Pd-nanoparticles altered kiwifruit pollen morphology and entered the grains more rapidly and to a greater extent than soluble Pd(II). At particulate Pd concentrations well below those of soluble Pd(II), pollen grains experienced rapid losses in endogenous calcium and pollen plasma membrane damage was induced. This resulted in severe inhibition and subsequent cessation of pollen tube emergence and elongation at particulate Pd concentrations as low as 0.4 mg L−1. Particulate Pd emissions related to automobile traffic have been increasing and are accumulating in the environment. This could seriously jeopardize in vivo pollen function, with impacts at an ecosystem level. Nanoparticulate Pd – which resembles emissions from automobile catalysts – affects pollen to a higher extent than soluble Pd.
اظهر المزيد [+] اقل [-]Effects of biochar and greenwaste compost amendments on mobility, bioavailability and toxicity of inorganic and organic contaminants in a multi-element polluted soil
2010
Applying amendments to multi-element contaminated soils can have contradictory effects on the mobility, bioavailability and toxicity of specific elements, depending on the amendment. Trace elements and PAHs were monitored in a contaminated soil amended with biochar and greenwaste compost over 60 days field exposure, after which phytotoxicity was assessed by a simple bio-indicator test. Copper and As concentrations in soil pore water increased more than 30 fold after adding both amendments, associated with significant increases in dissolved organic carbon and pH, whereas Zn and Cd significantly decreased. Biochar was most effective, resulting in a 10 fold decrease of Cd in pore water and a resultant reduction in phytotoxicity. Concentrations of PAHs were also reduced by biochar, with greater than 50% decreases of the heavier, more toxicologically relevant PAHs. The results highlight the potential of biochar for contaminated land remediation.
اظهر المزيد [+] اقل [-]Identification of a phytotoxic photo-transformation product of diclofenac using effect-directed analysis
2010
Schulze, Tobias | Weiss, Sara | Schymanski, Emma | Ohe, Peter Carsten von der | Schmitt-Jansen, Mechthild | Altenburger, R. | Streck, Georg | Brack, Werner
The pharmaceutical diclofenac (DCF) is released in considerably high amounts to the aquatic environment. Photo-transformation of DCF was reported as the main degradation pathway in surface waters and was found to produce metabolites with enhanced toxicity to the green algae Scenedesmus vacuolatus. We identified and subsequently confirmed 2-[2-(chlorophenyl)amino]benzaldehyde (CPAB) as a transformation product with enhanced toxicity using effect-directed analysis. The EC50 of CPAB (4.8 mg/L) was a factor of 10 lower than that for DCF (48.1 mg/L), due to the higher hydrophobicity of CPAB (log Kow = 3.62) compared with DCF (log Dow = 2.04) at pH 7.0.
اظهر المزيد [+] اقل [-]Phytotoxic Effects of Heavy Metal Contaminated Soil Reveal Limitations of Extract-Based Ecotoxicological Tests
2010
Gyuricza, Veronika | Fodor, Ferenc | Szigeti, Zoltán
Toxicity of contaminated soils cannot be assessed only by chemical analyses, therefore bioassays are increasingly used. Widely accepted ecotoxicological methods include organisms from all levels of the food-chain but plant-based ones are usually restricted to germination and growth tests. In our study the toxicity of heavy metal contaminated soil samples were examined not only by germination and bacterial tests of their extracts but also by the measurement of physiological parameters of two plant species (cucumber and wheat) that were grown directly on the contaminated substrate. Changes in chlorophyll concentration, stomatal conductance, fluorescence characteristics, and malondialdehyde (MDA) level (showing oxidative damage to lipids in leaves) undoubtedly indicated the mobilisation and toxic effect of contaminants. The results showed that the sensitivity of plant physiological parameters was higher than that of the extract-based ecotoxicological tests. Whereas these latter could not reveal the toxic effect of the highly contaminated soils the plants have reacted in a more complex way and their physiological parameters have changed significantly in all cases validating their use in such studies. The applied measurements also allow quicker and more reliable testing even under field conditions (stomatal conductance) or the detection of a more complex response if detailed analyses is needed (MDA, fluorescence imaging) thus underlining the importance of plant-based methods.
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