خيارات البحث
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High inter-species differences of 12378-polychlorinated dibenzo-p-dioxin between humans and mice
2020
Dong, Zhaomin | Ben, Yujie | Li, Yu | Li, Tong | Wan, Yi | Hu, Jianying
Although huge interspecies differences in the response to dioxins have been acknowledged, toxic equivalency factors derived from rodent studies are often used to assess human health risk. To determine interspecies differences, we first developed a toxicokinetic model in humans by measuring dioxin concentrations in environmental and biomonitoring samples from Southern China. Significant positive correlations between dioxin concentrations in blood and age were observed for seven dioxin congeners, indicating an age-dependent elimination rate. Based on toxicokinetic models in humans, the half-lives of 15 dioxin congeners were estimated to be 1.60–28.55 years. In consideration that the highest contribution to total toxic equivalency in blood samples was by 12378-polychlorinated dibenzo-p-dioxin (P₅CDD), this study developed a physiologically based pharmacokinetic (PBPK) model of 12378-P₅CDD levels in the liver, kidney, and fat of C57/6J mice exposed to a single oral dose, and the half-life was estimated to be 26.1 days. Based on estimated half-lives in humans and mice, we determined that the interspecies difference of 12378-P₅CDD was 71, much higher than the default usually used in risk assessment. These results could reduce the uncertainty human risk assessment of 12378-P₅CDD, and our approach could be used to estimate the interspecies differences of other dioxin congeners.
اظهر المزيد [+] اقل [-]Constant light exposure causes oocyte meiotic defects and quality deterioration in mice
2020
Zhang, Huiting | Yan, Ke | Sui, Lumin | Nie, Junyu | Cui, Kexin | Liu, Jiahao | Zhang, Hengye | Yang, Xiaogan | Lu, Kehuan | Liang, Xingwei
Artificial light at night (ALAN) exposes us to prolonged illumination, that adversely affects female reproduction. However, it remains to be clarified how prolonged light exposure affects oocyte meiotic maturation and quality. To this end, we exposed female mice to a constant light (CL) of 250 lux for different durations. Our findings showed that CL exposure for 7 weeks reduced the oocyte maturation rate. Meanwhile, CL exposure caused greater abnormalities in spindle assembly and chromosome alignment and a higher rate of oocyte aneuploidy than the regular light dark cycle. CL exposure also induced oxidative stress and caused mitochondrial dysfunction, which resulted in oocyte apoptosis and autophagy. Notably, our results showed that CL exposure reduced the levels of α-tubulin acetylation, DNA methylation at 5 mC, RNA methylation at m⁶A and histone methylation at H3K4me2 but increased the levels of histone methylation at H3K27me2 in oocytes. In summary, our findings demonstrate that constant bright light exposure causes oocyte meiotic defects and reduces cytoplasmic quality. These results extend the current understanding of ALAN-mediated defects in female reproduction.
اظهر المزيد [+] اقل [-]PCB52 exposure alters the neurotransmission ligand-receptors in male offspring and contributes to sex-specific neurodevelopmental toxicity
2020
Zhao, Dong | Wang, Qi | Zhou, Wen-Tao | Wang, Li-Bin | Yu, Hao | Zhang, Kai-Kai | Chen, Li-Jian | Xie, Xiao-Li
Polychlorinated biphenyls (PCBs) in the air are predominantly the less chlorinated congeners. Non-dioxin-like (NDL) low-chlorinated PCBs are more neurotoxic, and cause neurodevelopmental and neurobehavioral alterations in humans. However, the underlying mechanisms for this neurodevelopmental toxicity remain unknown. In the present study, Wistar rats were treated by gavage with PCB52 (1 mg/kg body weight) or corn oil from gestational day 7 to postnatal day 21. Both the body lengths and weights of the suckling rats at birth were significantly decreased by PCB52 treatment, suggesting developmental toxicity. Although no obvious histopathological changes were observed in the brain, using RNA-sequencing, 208 differentially expressed genes (DEGs) were identified in the striatum of PCB52-treated male offspring, while just 13 DEGs were identified in female offspring, suggesting sex-specific effects. Furthermore, using Gene Ontology enrichment analysis, neurodevelopmental processes, neurobehavioral alterations, and neurotransmission changes were enriched from the 208 DEGs in male offspring. Similarly, using Kyoto Encyclopedia of Genes and Genomes enrichment analysis, neuroactive ligand receptor interactions and multiple synapse pathways were enriched in male offspring, implying dysfunction of the neurotransmission system. Reductions in the protein expressions of these ligand receptors were also identified in the striatum, cerebral cortex, and hippocampus using western blotting methods. Taken together, our findings indicate that PCB52 exposure during gestation and lactation results in the abnormal expression of neurotransmission ligand-receptors in male offspring with a sex bias, and that this may contribute to neurodevelopmental toxicity.
اظهر المزيد [+] اقل [-]Are we underestimating microplastic abundance in the marine environment? A comparison of microplastic capture with nets of different mesh-size
2020
Lindeque, Penelope K. | Cole, Matthew | Coppock, Rachel L. | Lewis, Ceri N. | Miller, Rachael Z. | Watts, Andrew J.R. | Wilson-McNeal, Alice | Wright, Stephanie L. | Galloway, Tamara S.
Microplastic debris is ubiquitous and yet sampling, classifying and enumerating this prolific pollutant in marine waters has proven challenging. Typically, waterborne microplastic sampling is undertaken using nets with a 333 μm mesh, which cannot account for smaller debris. In this study, we provide an estimate of the extent to which microplastic concentrations are underestimated with traditional sampling. Our efforts focus on coastal waters, where microplastics are predicted to have the greatest influence on marine life, on both sides of the North Atlantic Ocean. Microplastic debris was collected via surface trawls using 100, 333 and 500 μm nets. Our findings show that sampling using nets with a 100 μm mesh resulted in the collection of 2.5-fold and 10-fold greater microplastic concentrations compared with using 333 and 500 μm meshes respectively (P < 0.01). Based on the relationship between microplastic concentrations identified and extrapolation of our data using a power law, we estimate that microplastic concentrations could exceed 3700 microplastics m⁻³ if a net with a 1 μm mesh size is used. We further identified that use of finer nets resulted in the collection of significantly thinner and shorter microplastic fibres (P < 0.05). These results elucidate that estimates of marine microplastic concentrations could currently be underestimated.
اظهر المزيد [+] اقل [-]Coastal zone use influences the spatial distribution of microplastics in Hangzhou Bay, China
2020
Wang, Ting | Hu, Menghong | Song, Lili | Yu, Jun | Liu, Ruijuan | Wang, Shixiu | Wang, Zhifu | Sokolova, Inna M. | Huang, Wei | Wang, Youji
Microplastic pollution in estuarine and coastal environments has recently been characterised in several countries but few researchers have addressed the influence of different forms of coastal zone use on the distribution of microplastic. Here, microplastic particles were sampled in Hangzhou Bay, which is heavily influenced by a range of human activities, and their abundance, size, and polymer type characterised. The abundance of microplastics was 0.14 ± 0.12 items/m³ in water, 84.3 ± 56.6 items/kg dry weight of sediment, and between 0.25 ± 0.14 and 1.4 ± 0.37 items/individual in biota. These results show that Hangzhou Bay has a low level of microplastic contamination compared to other coastal systems in China, although abundance was spatially variable within the bay; relatively higher microplastic abundances were found in the southern area of the bay, which has adjacent industrial and urban land-use zones, while lower abundances were observed in the central and northern bay areas where mariculture, fisheries, and mineral and energy industries are most common. The relatively low microplastic abundance observed in the biota samples is consistent with the generally low values for the seawater and sediment samples. Pellets were the most common of four particle-shape classes (fibres, fragments, films, and pellets) in surface seawater, while fibres were most abundant in sediment and biota. Smaller-sized microplastics (<1.0 mm) were dominant in all samples. Microplastics in the surface seawater were dominated by low-density polypropylene and polyethylene particles, while rayon was dominant in the sediment and biota samples. Our results demonstrate that regional variability in anthropogenic activity and land-use are important controls on the spatial pattern of microplastic pollution in Hangzhou Bay.
اظهر المزيد [+] اقل [-]Spatial distribution of heavy metal contamination in mollisol dairy farm
2020
Qi, Zheng | Gao, Xi | Qi, Yue | Li, Jinlong
To accurately visualize the spatial distribution of heavy metal pollution and provide basic information on soil remediation in dairy farm, Geographic Information System (GIS) is used for optimization of sample collection and data analysis. Based on GIS technology, dairy manure, 10 cm-depth surface soil, 50 cm-depth sub soil, and surface water samples were collected from dairy farm in Dulbert Mongolian Autonomous County, Daqing City, Heilongjiang Province in China. The spatial distribution and assessment of heavy metals were performed by using GIS inverse distance weighted interpolation and pollution index method. The single factor pollution index value of As element in the soil was found to indicate the class of extreme contamination, whereas Ni in both surface water inside and outside the farm, and Sb in the cow drinking water were assigned to the level of moderate contamination. The comprehensive pollution index implied serious contamination for soil samples, slight contamination for water samples and safety for manure samples, respectively. Comprehensive score for heavy metal elements followed the orders of As>Zn>Cr>Ni>Cu>Pb>Cd>Hg. The horizontal pollution that mainly occurred in the middle and east regions was increased from north to south, and west to east district. Historically, the dairy farm belonged to heavily polluted saline-alkali soil, where the heavy metals might enter the food chain through transportation from soil to water, the cows, and eventually to the milk and human body. Visualizing spatial distribution of heavy metal contamination by using GIS technology will be of significance to provide useful information for soil remediation of dairy farm.
اظهر المزيد [+] اقل [-]Potassium regulates the growth and toxin biosynthesis of Microcystis aeruginosa
2020
He, Yixin | Ma, Jianrong | Joseph, Vanderwall | Wei, Yanyan | Liu, Mengzi | Zhang, Zhaoxue | Li, Guo | He, Qiang | Li, Hong
Potassium (K⁺) is the most abundant cation in phytoplankton cells, but its impact on Microcystis aeruginosa (M. aeruginosa) has not been fully documented. This study presents evidence of how K⁺ availability affects the growth, oxidative stress and microcystin (MC) production of M. aeruginosa. The iTRAQ-based proteomic analysis revealed that during K⁺ deficiency, serious oxidative damage occurred and the photosynthesis-associated and ABC transporter-related proteins in M. aeruginosa were substantially downregulated. In the absence of K⁺, a 69.26% reduction in cell density was shown, and both the photosynthesis and iron uptake were depressed, which triggered a declined production of ATP and expression of MC synthetases genes (mcyA, B and D), and MC exporters (mcyH). Through the impairment of both the MC biosynthesis and MC transportation out of cells, K⁺ depletion caused an 85.89% reduction of extracellular MC content at the end of the study. However, with increasing in the available K⁺ concentrations, photosynthesis efficiency, the expression of ABC-transporter proteins, and the transcription of mcy genes displayed slight differences compared with those in the control group. This work represents evidence that K⁺ availability can regulate the physiological metabolic activity of M. aeruginosa and K⁺ deficiency leads to depressed growth and MC production in M. aeruginosa.
اظهر المزيد [+] اقل [-]Evaluating the meteorological normalized PM2.5 trend (2014–2019) in the “2+26” region of China using an ensemble learning technique
2020
Qu, Linglu | Liu, Shijie | Ma, Linlin | Zhang, Zhongzhi | Du, Jinhong | Zhou, Yunhong | Meng, Fan
In recent years, implementation of aggressive and strict clean air policies has resulted in significant decline in observed PM₂.₅ concentration in the Beijing–Tianjin–Hebei (BTH) region and its surrounding areas (i.e., the “2 + 26” region). To eliminate the effects of interannual and seasonal meteorological variation, and to evaluate the effectiveness of emission abatement policies, we applied a boosted regression tree model to remove confounding meteorological factors. Results showed that the annual average PM2.5 concentration normalized by meteorology for the “2 + 26” region declined by 38% during 2014–2019 (i.e., from 96 to 60 μg/m³); however, the BTH region exhibited the most remarkable decrease in PM₂.₅ concentration (i.e., a 60% reduction). Certain seasonal trend in normalized PM₂.₅ level remained for four target subregions owing to the effects of anthropogenic emissions in autumn and winter. Although strong interannual variations of meteorological conditions were unfavorable for pollutant dispersion during the heating seasons of 2016–2018, the aggressive abatement policies were estimated to have contributed to reductions in normalized PM₂.₅ concentration of 19%, 10%, 19%, and 17% in the BTH, Henan, Shandong, and Shanxi subregions, respectively. Our study eliminated the meteorological contribution to concentration variation and confirmed the effectiveness of the implemented clean air policies.
اظهر المزيد [+] اقل [-]The impact of anti-sea lice pesticides, azamethiphos and deltamethrin, on European lobster (Homarus gammarus) larvae in the Norwegian marine environment
2020
Parsons, Aoife E. | Escobar-Lux, Rosa H. | Sævik, Pål Næverlid | Samuelsen, Ole B. | Agnalt, Ann-Lisbeth
Anti-sea lice pesticides, used in the salmonid aquaculture industry, are a growing environmental concern due to their potential to adversely affect non-target crustaceans. Azamethiphos and deltamethrin are two bath treatment pesticides used on salmon farms in Norway, however, limited information is available on their impact on European lobster (Homarus gammarus) larvae in the Norwegian marine environment. Here, we firstly report the lethal (LC₅₀) and effective (EC₅₀) concentrations of azamethiphos and deltamethrin for stage I and stage II larvae, following 1-h exposures. Using a hydrodynamic model, we also modelled the dispersal of both compounds into the marine environment around selected Norwegian farms and mapped the potential impact zones (areas that experience LC₅₀ and EC₅₀ concentrations) around each farm. Our data shows that azamethiphos and deltamethrin are acutely toxic to both larval stages, with LC₅₀ and EC₅₀ values below the recommended treatment concentrations. We also show that the azamethiphos impact zones around farms were relatively small (mean area of 0.04–0.2 km²), however deltamethrin impact zones covered much larger areas (mean area of 21.1–39.0 km²). These findings suggest that deltamethrin poses a significant risk to European lobster in the Norwegian marine environment while the impact of azamethiphos may be less severe.
اظهر المزيد [+] اقل [-]Strong sorption of two fungicides onto biodegradable microplastics with emphasis on the negligible role of environmental factors
2020
Jiang, Mengyun | Hu, Liyang | Lu, Anxiang | Liang, Gang | Lin, Zuhong | Zhang, Tingting | Xu, Li | Li, Bingru | Gong, Wenwen
Microplastics have attracted much attention in recent years because they are able to interact with other pollutants including pesticides, with implications for the potential risks to biota. However, the sorption behavior of pesticides on microplastics, especially on biodegradable microplastics which are promising alternatives to conventional polymers, has been insufficiently studied. In this study, triadimefon and difenoconazole were selected as model triazole fungicides, and their sorption behavior on a typical biodegradable microplastics (PBS: polybutylene succinate) and two conventional polyethylene (PE) and polyvinyl chloride (PVC) microplastics was investigated with batch experiments in an aqueous solution. PBS presented the highest sorption capacity for triadimefon (104.2 ± 4.8 μg g⁻¹) and difenoconazole (192.8 ± 2.3 μg g⁻¹), which was 1.8- and 1.3-fold that on PE and 4.4- and 7.4-fold that of PVC, respectively. The results of sorption kinetic and isotherm modeling were better fit by a pseudo-second order model and linear model, respectively. More importantly, the effects of environmental factors (pH, salinity and dissolved organic matter) on the sorption behavior were investigated. Fungicide sorption on PBS was generally not affected by salinity, pH or dissolved organic matter. However, in contrast, salinity and dissolved organic matter both significantly decreased sorption on PE and PVC. The results showed that not only the sorption capacities of biodegradable microplastics but also their responses to environmental factors are quite different from those of conventional microplastics. This finding highlights the importance of the role played by biodegradable microplastics in the accumulation and transportation of organic pollutants.
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