This research aims to study the effect of Mycorrizal fungus and Plant-Growth-Promoting Bacteria (PGPB) on Cadmium (Cd) uptake by one-year-old Eucalyptus Camaldulensis seedlings. The treatments have involved three levels of heavy metal (0, 30, and 60 mg/kg) for Cd, and three bacterial levels (no bacteria (B0), Bacillus (Ba105), and Pseudomonas (Ps36, Ps448)), inoculated with mycorrhizal fungus Glomus mosseae (M1) and non-inoculated with fungus (M0). Results show that absorption of these elements in plant increased as Cd concentration in soil became more. Inoculation by Ps448 bacteria had an incremental effect on Cd uptake by 90%, compared to the non-inoculated (control) samples. Moreover, inoculation of the plants with mycorrhizal fungus increased Cd uptake by 24%, compared to the control. Also, it has been observed that plant resistance to metal stress and plant growth under such conditions ascended in treatments wherein inoculation happened with mycorrhizal fungus and bacteria. The highest Cd heavy metal uptake has been observed in Eucalyptus (shoots and roots), treatment (C2B2M1) with 648.19 micrograms per one seedling in pot. According to the obtained results, Eucalyptus with biological factors (fungi and bacteria) has the ability to clean and purify the contaminated soil with Cd heavy metal.

In this study, among the 21 diesel-degrading bacteria that were isolated from an oil-polluted area in Fars (Iran), 6 bacterial strains were tested for their capability to metabolize and grow on diesel oil by degrading its hydrocarbons content. The biochemical characteristics and 16S rRNA sequence analysis of diesel-degrading bacteria showed that these strains were related to the genus Pseudomonas. Among the six isolates, five strains (L1, I2, D1, D2, and G1) were clustered with Pseudomonas aeruginosa, whereas only one strain (K3) was clustered with Pseudomonas fragi. Gas chromatographic (GC) analysis of the diesel oil that was remaining in the culture medium after 10 days of culture at 30°C showed that P. aeruginosa I2 presented the highest growth rate and diesel-oil degradation (88%) between all isolates. P. aeruginosa I2 also presented the best emulsification activity, but the best hydrophobicity was seen in P. aeruginosa G1. By applying these bacteria in bioremediation processes, diesel oil contamination in soil can be counteracted.