peer reviewed | Contamination of land and aquatic ecosystems with heavy metals (HMs) is a global issue having the persistent potential to damage the quality of food and water. In the present study, Tagetes erecta L. plants were used to assess their potential to uptake HMs from wastewater. Plants were grown in soil for 20 days and then transplanted in hydroponic system containing Hoagland nutrient solution. After more than 15 days of growth, plants were then subjected to wastewater from tannery and surgical industries in different concentrations ranging from 25 to 100% in combination of citric acid (5 and 10 mM). After 6 weeks of treatment, plants were collected and segmented into roots, stem, and leaves for characterizing the morphological properties including plant height, roots length, fresh and dry mass of roots, stem, and leaves. For evaluation of the effect of wastewater on the plants, photosynthetic pigments; soluble proteins; reactive oxygen species (ROS); antioxidant enzymes SOD, POD, CAT, and APX; and metal accumulation were analyzed. Application of industrial wastewater revealed a significant effect on plant morphology under wastewater treatments. Overall growth and physiological attributes of plant decreased, and metal accumulation enhanced with increasing concentration of wastewater. Similarly, the production of ROS and antioxidant enzymes were also increased. Chlorophyll, protein content, and enzyme production enhanced with CA (5 and 10 mM) mediation; however, ROS production and EL were reduced. Metals analysis showed that the maximum accumulation of Pb was in roots, while Cr and Ni in the stem which further increased under CA mediation. Overall, the metal accumulation ability was in the order of Pb > Ni > Cr under CA.

As one of the most common ways to get rid of municipal waste, landfill leachate, waste with complicated compositions and high levels of contaminants, has become a significant threat to the world's environment. Here, the impact of sewage sludge (SS) and derived-biochar (SSB) amendments on the immobilization and potential mobility of heavy metals in a contaminated soil-plant system was investigated. The sequential fractionation findings showed that using SS-2%, SSB-2%, and SSBC-1% reduced the potential mobility of heavy metals while increasing the residual fraction in polluted soils. The translocation and bioconcentration factors showed that heavy metals were slightly transferred into shoots from roots and lowered accumulation in roots from contaminated soils. Fourier transform infrared (FTIR) and X-ray photoelectron spectrum (XPS) comprehensive characterization results indicated the significant role of applied amendments for heavy metals transformation from the exchangeable-soluble fractions to the least available form by lowering their mobility to confirm the adsorption-based complexes, which results in the surface adsorption of heavy metals with functional groups. The electron paramagnetic resonance (EPR) results indicated the dominance of reactive oxygen species (ROS) in the intracellular formation of hydroxyl radicals (•OH) in maize plant roots and shoots. ROS (•OH) generation plays a critical influence in the interaction between the physiological processes of plants and heavy metals. Moreover, all the amendments increased maize growth and biomass production. Our study suggests that alone and combined application of SS and SSB have great potential to remediate heavy metals contaminated soil for environmental sustainability.

The role of hydrogen sulfide (H₂S) is well known in the regulation of abiotic stress such as toxic heavy metal. However, mechanism(s) lying behind this amelioration are still poorly known. Consequently, the present study was focused on the regulation/mitigation of hexavalent chromium (Cr(VI) toxicity by the application of H₂S in wheat and rice seedlings. Cr(VI) induced accumulation of reactive oxygen species and caused protein oxidation which negatively affect the plant growth in both the cereal crops. We noticed that Cr(VI) toxicity reduced length of wheat and rice seedlings by 21% and 19%, respectively. These reductions in length of both the cereal crops were positively related with the down-regulation in the ascorbate-glutathione cycle, and were recovered by the application NaHS (a donor of H₂S). Though exposure of Cr(VI) slightly stimulated sulfur assimilation but addition of H₂S further caused enhancement in sulfur assimilation, suggesting its role in the H₂S-mediated Cr(VI) stress tolerance in studied cereal crops. Overall, the results revealed that H₂S renders Cr(VI) stress tolerance in wheat and rice seedlings by stimulating sulfur assimilation and ascorbate-glutathione which collectively reduce protein oxidation and thus, improved growth was observed.

Arsenic (As) contamination and bioaccumulation are a serious threat to agricultural plants. To address this issue, we checked the efficacy of As tolerant plant growth promoting bacteria (PGPB), zinc oxide nanoparticles (ZnO NPs) and oxalic acid (OA) in Luffa acutangula grown on As rich soil. The selected most As tolerant PGPB i.e Providencia vermicola exhibited plant growth promoting features i.e solubilzation of phosphate, potassium and siderophores production. Innovatively, we observed the synergistic effects of P. vermicola, ZnO NPs (10 ppm) and OA (100 ppm) in L. acutangula grown on As enriched soil (150 ppm). Our treatments both as alone and in combination alleviated As toxicity exhibited by better plant growth and metabolism. Results revealed significantly enhanced photosynthetic pigments, proline, relative water content, total sugars, proteins and indole acetic acid along with As amelioration in L. acutangula. Furthermore, upregulated plant resistance was manifested with marked reduction in the lipid peroxidation and electrolyte leakage and pronounced antagonism of As and zinc content in leaves under toxic conditions. These treatments also improved level of nutrients, abscisic acid and antioxidants to mitigate As toxicity. This marked improvement in plants’ defense mechanism of treated plants under As stress is confirmed by less damaged leaves cell structures observed through the scanning electron micrographs. We also found substantial decrease in the As bioaccumulation in the L. acutangula shoots and roots by 40 and 58% respectively under the co-application of P. vermicola, ZnO NPs and OA in comparison with control. Moreover, the better activity of soil phosphatase and invertase was assessed under the effect of our application. These results cast a new light on the application of P. vermicola, ZnO NPs and OA in both separate and combined form as a feasible and ecofriendly tool to alleviate As stress in L. acutangula.

Antimicrobial nanomaterials provide numerous opportunities for the synthesis of next-generation sustainable water disinfectants. Using the keywords graphene and water disinfection and graphene antibacterial activity, a detailed search of the Scopus database yielded 198 and 1433 studies on using graphene for water disinfection applications and graphene antibacterial activity in the last ten years, respectively. Graphene family nanomaterials (GFNs) have emerged as effective antibacterial agents. The current innovations in graphene-, graphene oxide (GO)-, reduced graphene oxide (rGO)-, and graphene quantum dot (GQD)-based nanocomposites for water disinfection, including their functionalization with semiconductor photocatalysts and metal and metal oxide nanoparticles, have been thoroughly discussed in this review. Furthermore, their novel application in the fabrication of 3D porous hydrogels, thin films, and membranes has been emphasized. The physicochemical and structural properties affecting their antibacterial efficiency, such as sheet size, layer number, shape, edges, smoothness/roughness, arrangement mode, aggregation, dispersibility, and surface functionalization have been highlighted. The various mechanisms involved in GFN antibacterial action have been reviewed, including the mechanisms of membrane stress, ROS-dependent and -independent oxidative stress, cell wrapping/trapping, charge transfer, and interaction with cellular components. For safe applications, the potential biosafety and biocompatibility of GFNs in aquatic environments are emphasized. Finally, the current limitations and future perspectives are discussed. This review may provide ideas for developing efficient and practical solutions using graphene-, GO-, rGO-, and GQD-based nanocomposites in water disinfection by rationally employing their unique properties.

Plants detoxify toxic metal(loid)s by accumulating diverse metabolites. Beside scavenging excess reactive oxygen species (ROS) induced by metal(loid)s, some metabolites chelate metal(loid) ions. Classically, thiol-containing compounds, especially glutathione (GSH) and phytochelatins (PCs) are thought to be the major chelators that conjugate with metal(loid)s in the cytoplasm followed by transport and sequestration in the vacuole. In addition to this classical detoxification pathway, a role for secondary metabolites in metal(loid) detoxification has recently emerged. In particular, anthocyanins, a kind of flavonoids with ROS scavenging potential, contribute to enhanced arsenic tolerance in several plant species. Evidence is accumulating that, in analogy to GSH and PCs, anthocyanins may conjugate with arsenic followed by vacuolar sequestration in the detoxification event. Exogenous application or endogenous accumulation of anthocyanins enhances arsenic tolerance, leading to improved plant growth and productivity. The application of some plant hormones and signaling molecules stimulates endogenous anthocyanin synthesis which confers tolerance to arsenic stress. Anthocyanin biosynthesis is transcriptionally regulated by several transcription factors, including myeloblastosis (MYBs). The light-regulated transcription factor elongated hypocotyl 5 (HY5) also affects anthocyanin biosynthesis, but its role in arsenic tolerance remains elusive. Here, we review the mechanism of arsenic detoxification in plants and the potential role of anthocyanins in arsenic tolerance beyond the classical points of view. Our analysis proposes that anthocyanin manipulation in crop plants may ensure sustainable crop yield and food safety in the marginal lands prone to arsenic pollution.

Artificial light at night (ALAN) alters circadian rhythms in animals and therefore can be a source of environmental stress affecting their physiology and behaviour. The impact of ALAN can be related to the increased light level, but also to the spectral composition of night lighting. Previous research showed that many species can be particularly sensitive to the LED light, but it is unclear if they respond to its broad spectrum or specifically to the blue light wavelength. In this study, we tested whether dim ALAN (2 lx) differing in the spectral quality (warm white LED, blue LED, high-pressure sodium HPS light) modifies behaviour and changes oxidative status in two nocturnal freshwater shredder species: Dikerogammarus villosus and Gammarus jazdzewskii (Gammaroidea, Amphipoda). Our experiment revealed that ALAN, irrespective of its spectral quality, did not affect the oxidative stress markers in cells (the level of reactive oxygen species and lipid peroxidation). However, ALAN changed the gammarid behaviour in a species-specific manner, which can potentially reduce the fitness of the shredders. Dikerogammarus villosus avoided all types of light compared to darkness. Therefore, confined to the shelter, D. villosus may have fewer opportunities to forage and/or mate. Gammarus jazdzewskii was sensitive only to the narrow-spectrum blue light, but did not respond to the HPS and white LED light. Avoidance is a typical response of gammarids to natural light, thus the disruption of this behaviour in the presence of common ALAN sources can increase the predation risk in this species. To summarize, behavioural modifications induced by ALAN seem more pronounced than changes in physiology and can constitute the main driver of disturbances in the processing of organic matter in freshwater ecosystems by invertebrate shredders.

Growing evidence recommends that radiofrequency radiations might be a new type of environmental pollutant. The consequences of RFR on the human immune system have gained considerable interest in recent years, not only to examine probable negative effects on health but also to understand if RFR can modulate the immune response positively. Although several studies have been published on the immune effects of RFR but no satisfactory agreement has been reached. Hence this review aims to evaluate the RFR modulating impacts on particular immune cells contributing to various innate or adaptive immune responses. In view of existing pieces of evidence, we have suggested an intracellular signaling cascade responsible for RFR action. The bio-effects of RFR on immune cell morphology, viability, proliferation, genome integrity, and immune functions such as ROS, cytokine secretion, phagocytosis, apoptosis, etc. are discussed. The majority of existing evidence point toward the possible shifts in the activity, number, and/or function of immunocompetent cells, but the outcome of several studies is still contradictory and needs further studies to reach a conclusion. Also, the direct association of experimental studies to human risks might not be helpful as exposure parameters vary in real life. On the basis of recent available literature, we suggest that special experiments should be designed to test each particular signal utilized in communication technologies to rule out the hypothesis that longer exposure to RFR emitting devices would affect the immunity by inducing genotoxic effects in human immune cells.

RNA N⁶-methyladenosine (m⁶A) modification regulates the cell stress response and homeostasis, but whether titanium dioxide nanoparticle (nTiO₂)-induced acute pulmonary injury is associated with the m⁶A epitranscriptome and the underlying mechanisms remain unclear. Here, the potential association between m⁶A modification and the bioeffects of several engineered nanoparticles (nTiO₂, nAg, nZnO, nFe₂O₃, and nCuO) were verified thorough in vitro experiments. nFe₂O₃, nZnO, and nTiO₂ exposure significantly increased the global m⁶A level in A549 cells. Our study further revealed that nTiO₂ can induce m⁶A-mediated acute pulmonary injury. Mechanistically, nTiO₂ exposure promoted methyltransferase-like 3 (METTL3)-mediated m⁶A signal activation and thus mediated the inflammatory response and IL-8 release through the degeneration of anti-Mullerian hormone (AMH) and Mucin5B (MUC5B) mRNAs in a YTH m⁶A RNA-binding protein 2 (YTHDF2)-dependent manner. Moreover, nTiO₂ exposure stabilized METTL3 protein by the lipid reactive oxygen species (ROS)-activated ERK1/2 pathway. The scavenging of ROS with ferrostatin-1 (Fer-1) alleviates the ERK1/2 activation, m⁶A upregulation, and the inflammatory response caused by nTiO₂ both in vitro and in vivo. In conclusion, our study demonstrates that m⁶A is a potential intervention target for alleviating the adverse effects of nTiO₂-induced acute pulmonary injury in vitro and in vivo, which has far-reaching implications for protecting human health and improving the sustainability of nanotechnology.

Ethylene regulates plant root growth and resistance to environment stress. However, the role and mechanism of ethylene signaling in response to Cd stress in rice remains unclear. Here, we revealed that ethylene signaling plays a positive role in the resistance of rice to Cd toxicity. Blocking the ethylene signal facilitated root elongation under normal conditions, but resulted in severe oxidative damage and inhibition of root growth under Cd stress. Conversely, ethylene signal enhancement by EIN2 overexpression caused root bending, similar to the response of roots to Cd stress, and displayed higher Cd tolerance than the wildtype (WT) plants. Comparative transcriptome analysis indicated EIN2-mediated upregulation of genes involved in flavonoid biosynthesis and peroxidase activity under Cd stress. The synthesis of phenolic acids and flavonoids were positively regulated by ethylene. Thus, the ein2 (ethylene insensitive 2) mutants displayed lower ROS scavenging capacity than the WT. Moreover, a significant increase in Cd accumulation and relatively increased apoplastic flow were observed in the root apex of the ein2 mutant compared with the WT plants. Overall, EIN2-mediated Cd resistance in rice is mediated by the upregulation of flavonoid biosynthesis and peroxidase activity to induce ROS scavenging, and apoplastic transport barrier formation reduces Cd uptake.