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Sub-lethal and lethal toxicities of elevated CO2 on embryonic, juvenile, and adult stages of marine medaka Oryzias melastigma
2018
Lee, Changkeun | Kwon, Bong-Oh | Hong, Seongjin | Noh, Junsung | Lee, Junghyun | Ryu, Jongseong | Kang, Seong-Gil | Khim, Jong Seong
The potential leakage from marine CO2 storage sites is of increasing concern, but few studies have evaluated the probable adverse effects on marine organisms. Fish, one of the top predators in marine environments, should be an essential representative species used for water column toxicity testing in response to waterborne CO2 exposure. In the present study, we conducted fish life cycle toxicity tests to fully elucidate CO2 toxicity mechanism effects. We tested sub-lethal and lethal toxicities of elevated CO2 concentrations on marine medaka (Oryzias melastigma) at different developmental stages. At each developmental stage, the test species was exposed to varying concentrations of gaseous CO2 (control air, 5%, 10%, 20%, and 30%), with 96 h of exposure at 0–4 d (early stage), 4–8 d (middle stage), and 8–12 d (late stage). Sub-lethal and lethal effects, including early developmental delays, cardiac edema, tail abnormalities, abnormal pigmentation, and mortality were monitored daily during the 14 d exposure period. At the embryonic stage, significant sub-lethal and lethal effects were observed at pH < 6.30. Hypercapnia can cause long-term and/or delayed developmental embryonic problems, even after transfer back to clean seawater. At fish juvenile and adult stages, significant mortality was observed at pH < 5.70, indicating elevated CO2 exposure might cause various adverse effects, even during short-term exposure periods. It should be noted the early embryonic stage was found more sensitive to CO2 exposure than other developmental stages of the fish life cycle. Overall, the present study provided baseline information for potential adverse effects of high CO2 concentration exposure on fish developmental processes at different life cycle stages in marine ecosystems.
اظهر المزيد [+] اقل [-]17β-estradiol as precursors of Cl/Br-DBPs in the disinfection process of different water samples
2018
During chlorine disinfection process, reactions between the disinfectant and 17β-estradiol (E2) lead to the formation of halogenated disinfection byproducts (DBPs) which can be a risk to both ecosystem and human health. The degradation and transformation products of E2 in sodium hypochlorite (NaClO) disinfection processes of different water samples were investigated. The reaction kinetics research showed that the degradation rates of E2 were considerably dependent on the initial pH value and the types of water samples. In fresh water, synthetic marine aquaculture water and seawater, the reaction rate constant was 0.133 min−1, 2.067 min−1 and 2.592 min−1, respectively. The reasons for the above phenomena may be due to the different concentrations of bromide ions (Br−) in these three water samples which could promote the reaction between NaClO and E2. Furthermore, Br− could also cause the formation of brominated DBPs (Br-DBPs). The main DBPs, reaction centers and conceivable reaction pathways were explored. Seven halogenated DBPs have been observed including three chlorinated DBPs (Cl-DBPs) and four Br-DBPs. The active sites of E2 were found to be the pentabasic cyclic ring and the ortho position of the phenol moiety as well as C9-C10 position. The identified Cl/Br-DBPs were also confirmed in actual marine aquaculture water from a shrimp pond. The comparison of bio-concentration factors (BCF) values based on calculation of EPI-suite showed that the toxicities of the Br-DBPs were stronger than that of their chloride analogues. The absorbable organic halogens (AOX) analysis also suggested that the DBPs produced in the marine aquaculture water were more toxic than that in the fresh water system.
اظهر المزيد [+] اقل [-]Adsorption of perfluoroalkyl substances on microplastics under environmental conditions
2018
Llorca, Marta | Schirinzi, Gabriella | Martinez, Monica | Barceló, Damià | Farré, Marinella
Plastic debris has become an environmental problem during recent years. Among the plastic debris, microplastics (<5 mm; MPLs) imply an extra problem due to their capacity to enter into the fauna through ingestion. In this work, we study the capacity of three MPLs, that include high-density polyethylene (HDPE), polystyrene (PS) and polystyrene carboxylate (PS-COOH), to sorb 18 perfluoroalkyl substances (PFASs; including carboxylic acids, sulphonates and one sulphonamide) from the surrounding waters (freshwater and seawater).Conclusions drawn from the results are that perfluoro sulphonates and sulphonamides have more tendency to be sorbed onto MPLs. In addition, PS and PS-COOH have more affinity for PFASs than HDPE. Finally, the increment of conductivity and pH of the water decreases the exposure time that is necessary to reach equilibrium. However, the presence of salts decreases the tendency of PFASs to be sorbed onto plastic surfaces. These results highlight the problem associated with the presence of MPLs in inland and marine waters since toxic compounds can be sorbed onto surrounding plastics that could be ingested by aquatic fauna.
اظهر المزيد [+] اقل [-]Potential transfer of organic pollutants from littoral plastics debris to the marine environment
2018
León, Víctor M. | García, Inés | González, Emilia | Samper, Raquel | Fernández-González, Verónica | Muniategui-Lorenzo, Soledad
Plastic polymers act as passive samplers in air system and concentrate hydrophobic organic contaminants by sorption or specific interactions, which can be transported to other systems such as the marine environment. In this study plastic debris was sampled in the surrounding area of a Mediterranean lagoon in order to determine the concentration of persistent and emerging organic contaminants. More specifically, desorption of 91 regulated and emerging organic contaminants (polycyclic aromatic hydrocarbons, polychlorinated biphenyls, organochlorinated pesticides, current-use pesticides, personal care products, other pesticides and plastic additives) was characterized for the first 24 h from different polymers to seawater and the remaining content of these contaminants was also extracted by ultrasonic extraction with methanol. All samples were analyzed by Stir Bar Sorptive Extraction coupled to GC/MS. A significant fraction of sorbed contaminants in polymers was desorbed in the first 24 h, particularly for triazines and organophosphorus pesticides due to their lower hydrophobicity than other considered analytes. The remaining contaminants contained in plastics can be also transferred to seawater, sediments or biota. Considering 24 h desorbed fraction plus the remaining methanol extracted fraction, the highest transfer levels corresponded to personal care products, plastic additives, current-use pesticides and PAHs. This is the first study to show the relevance of the transport of organic contaminants on plastic debris from littoral areas to the marine environment.
اظهر المزيد [+] اقل [-]Spatial and vertical variations of perfluoroalkyl acids (PFAAs) in the Bohai and Yellow Seas: Bridging the gap between riverine sources and marine sinks
2018
Zhou, Yunqiao | Wang, Tieyu | Li, Qifeng | Wang, Pei | Li, Lei | Chen, Shuqin | Zhang, Yueqing | Kifāyatullāh, K̲h̲ān | Meng, Jing
Perfluoroalkyl acids (PFAAs) are being increasingly reported as emerging contaminants in riverine and marine settings. This study investigated the contamination level and spatial distribution of 17 PFAAs within the depth profile of the Bohai and Yellow Seas using newly detected sampling data from 49 sites (June 29 to July 14, 2016). Moreover, the riverine flux of 11 selected PFAAs in 33 rivers draining into the Bohai and Yellow Seas was estimated from previous studies (2002–2014) in order to establish the relationship between riverine sources and marine sinks. The results showed that the Bohai and Yellow Seas were commonly contaminated with PFAAs: total concentrations of PFAAs in the surface, middle, and bottom zones ranged from 4.55 to 556 ng L−1, 4.61–575 ng L−1, and 4.94–572 ng L−1, respectively. The predominant compounds were PFOA (0.55–449 ng L−1), PFBA (<LOQ-34.5 ng L−1), and PFPeA (<LOQ-54.3 ng L−1), accounting for 10.1–87.0%, 5.2–59.5%, and 0.6–68.6% of the total PFAAs, respectively. In general, the ∑PFAA concentrations showed a slightly decreasing trend with sampling depth. Contamination was particularly severe in Laizhou Bay, fed by the Xiaoqing River and an industrial park known for PFAA production. The total riverine PFAA mass flux into the Bohai and Yellow Seas was estimated to be 72.2 t y−1, of which 94.8% was carried by the Yangtze and Xiaoqing Rivers. As the concentration of short-chain PFAAs begins to rise in seawater, further studies on the occurrence and fate of short-chain PFAAs with special focus on effective control measures would be very timely, particularly in the Xiaoqing River and Laizhou Bay.
اظهر المزيد [+] اقل [-]Assessment of Cu sub-lethal toxicity (LC50) in the cold-water gorgonian Dentomuricea meteor under a deep-sea mining activity scenario
2018
Martins, Inês | Godinho, António | Goulart, Joana | Carreiro-Silva, Marina
Previous aquaria-based experiments have shown dissolution and leaching of metals, especially copper (Cu), from the simulated sediment plumes generated during mining activities resulting in a pronounced increase of Cu contamination in the surrounding seawater. Metals are bioavailable to corals with food, through ingestion (particulate phase) and through tissue-facilitated transport (passive diffusion). With corals being particularly vulnerable to metal contamination, resuspension of metal-bearing sediments during mining activities represents an important ecological threat. This study was undertaken to evaluate the impact of acute copper exposure (LC50;96 h) on the survival of the cold-water octocoral Dentomuricea aff. meteor. The experimental design was divided in two stages. In stage one, a Cu range-finding toxicity test was performed using Cu dilutions in filtered seawater with concentrations of 0 (control); 60; 150; 250; 450; 600 μg/L. Coral mortality was investigated visually based on the percent surface area of tissue changing from natural yellow colour to black colour indicative of tissue necrosis and death. In stage two, we used the results obtained in the range-finding experiment, to define sub-lethal Cu exposure treatments and exposed D. meteor to Cu concentration of 0 (control); 50; 100; 150; 200; 250 μg/L for 96 h. The corals physical conditions were inspected daily and seawater conditions recorded. Corals were considered dead when all of their tissue turned black. The LC50 value was calculated with regression analysis following Probits methodology. Our results indicate that Cu LC50;96 h for the octocoral D. meteor is 137 μg/L.
اظهر المزيد [+] اقل [-]Qualitative assessment to determine internal and external factors influencing the origin of styrene oligomers pollution by polystyrene plastic in coastal marine environments
2018
Kwon, Bum Gun | Chung, Seon-Yong | Park, Seung-Shik | Saido, Katsuhiko
The objective of this study is to investigate the qualitative contribution of internal and external factors of the area contaminated by polystyrene (PS) in coastal marine environments. This study is based on the extensive results of monitoring the styrene oligomers (SOs) present in sand and seawater samples along various coastlines of the Pacific Ocean. Here, anthropogenic SOs is derived from PS during manufacture and use, and can provide clues about the origin of SOs by PS pollution. The monitoring results showed that, if the concentration of SOs in water is higher than those concentrations in beach sand, this area could be affected by PS plastic caused by an external factor. On the other hand, if the concentration of SOs is higher in the beach sand, the region can be mainly influenced by PS plastic derived from its own area. Unlike the case of an external factor, in this case (internal influence), it is possible to take policy measures of the area itself for the PS plastic problem. Thus, this study is motivated by the need of policy measures to establish a specific alternative to the problems of PS plastic pollution in ocean environments.
اظهر المزيد [+] اقل [-]AOX contamination in Hangzhou Bay, China: Levels, distribution and point sources
2018
Xie, Ya-Wei | Chen, Lu-Jun | Liu, Rui | Tian, Jin-Ping
The parameter AOX (adsorbable organic halogens) indicates the total amount of organic halogens in an environment. Seawater and surface sediment samples from 12 sample sites in the Hangzhou Bay (HZB), China, were analyzed for AOX to investigate its contamination status. In this study, the AOX concentration ranged from 140.6 ± 45.6 μg/L to 716.1 ± 62.3 μg/L in seawater of the HZB, and from 11.3 ± 2.4 mg/kg to 112.7 ± 7.2 mg/kg in the sediment. Ocean currents, fluvial currents and the Yangtze River exerted profound influences on the distribution of AOX in the HZB. The point sources around the HZB, represented by wastewater treatment plants, discharged at least 645.4 t AOX into the HZB every year, most of which was generated by industrial activities rather than the human daily activities.
اظهر المزيد [+] اقل [-]Biological responses of two marine organisms of ecological relevance to on-going ocean acidification and global warming
2018
Gomiero, A. | Bellerby, R.G.J. | Manca Zeichen, M. | Babbini, L. | Viarengo, A.
Recently, there has been a growing concern that climate change may rapidly and extensively alter global ecosystems with unknown consequences for terrestrial and aquatic life. While considerable emphasis has been placed on terrestrial ecology consequences, aquatic environments have received relatively little attention. Limited knowledge is available on the biological effects of increments of seawater temperature and pH decrements on key ecological species, i.e., primary producers and/or organisms representative of the basis of the trophic web. In the present study, we addressed the biological effects of global warming and ocean acidification on two model organisms, the microbenthic marine ciliate Euplotes crassus and the green alga Dunaliella tertiocleta using a suite of high level ecological endpoint tests and sub-lethal stress measures. Organisms were exposed to combinations of pH and temperature (TR1: 7.9[pH], 25.5 °C and TR2: 7.8[pH], 27,0 °C) simulating two possible environmental scenarios predicted to occur in the habitats of the selected species before the end of this century. The outcomes of the present study showed that the tested scenarios did not induce a significant increment of mortality on protozoa. Under the most severe exposure conditions, sub-lethal stress indices show that pH homeostatic mechanisms have energetic costs that divert energy from essential cellular processes and functions. The marine protozoan exhibited significant impairment of the lysosomal compartment and early signs of oxidative stress under these conditions. Similarly, significant impairment of photosynthetic efficiency and an increment in lipid peroxidation were observed in the autotroph model organism held under the most extreme exposure condition tested.
اظهر المزيد [+] اقل [-]Does exposure to reduced pH and diclofenac induce oxidative stress in marine bivalves? A comparative study with the mussel Mytilus galloprovincialis and the clam Ruditapes philippinarum
2018
Munari, Marco | Matozzo, Valerio | Gagné, François | Chemello, Giulia | Riedl, Verena | Finos, Livio | Pastore, Paolo | Badocco, Denis | Marin, Maria Gabriella
CO2-driven acidification and emerging contaminants, such as pharmaceuticals, pose new threats for the maintenance of natural populations of marine organisms by interfering with their normal biochemical pathways and defences. The combined effects of seawater acidification, as predicted in climate change scenarios, and an emerging contaminant (the non-steroidal anti-inflammatory drug, NSAID, diclofenac) on oxidative stress-related parameters were investigated in the Mediterranean mussel Mytilus galloprovincialis and the Manila clam Ruditapes philippinarum. A flow-through system was used to carry out a three-week exposure experiment with the bivalves. First, the animals were exposed to only three pH values for 7 days. The pH was manipulated by dissolving CO2 in the seawater to obtain two reduced pH treatments (pH −0.4 units and pH −0.7 units), which were compared with seawater at the natural pH level (8.1). Thereafter, the bivalves were concomitantly exposed to the three experimental pH values and environmentally relevant concentrations of diclofenac (0.00, 0.05 and 0.50 μg/L) for an additional 14 days. The activities of superoxide dismutase, catalase and cyclooxygenase, and lipid peroxidation and DNA strand-break formation were measured in both the gills and digestive gland after 7, 14 and 21 days of exposure to each experimental condition. The results show that the biochemical parameters measured in both the mussels and clams were more influenced by the reduced pH than by the contaminant or the pH*contaminant interaction, although the biomarker variation patterns differed depending on the species and tissues analysed. Generally, due to increases in its antioxidant defence, M. galloprovincialis was more resistant than R. philippinarum to both diclofenac exposure and reduced pH. Conversely, reduced pH induced a significant decrease in COX activity in both the gills and digestive gland of clams, possibly resulting in the increased DNA damage observed in the digestive gland tissue.
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