In this study, Bacillus sp. strain AVPP64 was isolated from diuron-contaminated soil. It showed 4-nitroaniline (4-NA) degradation, pesticide tolerance, and self-nutrient integration via nitrogen (N)-fixation and phosphate (P)-solubilization. The rate constant (k) and half-life period (t₁/₂) of 4-NA degradation in the aqueous medium inoculated with strain AVPP64 were observed to be 0.445 d⁻¹ and 1.55 d, respectively. Nevertheless, in the presence of chlorpyrifos, profenofos, atrazine and diuron pesticides, strain AVPP64 degraded 4-NA with t₁/₂ values of 2.55 d, 2.26 d, 2.31 d and 3.54 d, respectively. The strain AVPP64 fixed 140 μg mL⁻¹ of N and solubilized 103 μg mL⁻¹ of P during the presence of 4-NA. In addition, strain AVPP64 produced significant amounts of plant growth-promoting metabolites like indole 3-acetic acid, siderophores, exo-polysaccharides and ammonia. In the presence of 4-NA and various pesticides, strain AVPP64 greatly increased the growth and biomass of Vigna radiata and Crotalaria juncea plants. These results revealed that Bacillus sp. strain AVPP64 can be used as an inoculum for bioremediation of 4-NA contaminated soil and sustainable crop production even when pesticides are present.

The impact of siderophore produced by arsenic-resistant bacterium Pseudomonas PG12 on FeAsO4 dissolution and plant growth were examined. Arsenic-hyperaccumulator Pteris vittata was grown for 7 d in 0.2-strength Fe-free Hoagland solution containing FeAsO4 mineral and PG12-siderophore or fungal-siderophore desferrioxamine B (DFOB). Standard siderophore assays indicated that PG12-siderophore was catecholate-type. PG12-siderophore was more effective in promoting FeAsO4 dissolution, and Fe and As plant uptake than DFOB. Media soluble Fe and As in PG12 treatment were 34.6 and 3.07 μM, 1.6- and 1.4-fold of that in DFOB. Plant Fe content increased from 2.93 to 6.24 g kg−1 in the roots and As content increased from 14.3 to 78.5 mg kg−1 in the fronds. Besides, P. vittata in PG12 treatment showed 2.6-times greater biomass than DFOB. While P. vittata fronds in PG12 treatment were dominated by AsIII, those in DFOB treatment were dominated by AsV (61–77%). This study showed that siderophore-producing arsenic-resistant rhizobacteria may have potential in enhancing phytoremediation of arsenic-contaminated soils.

In this study, two proficient Cadmium (Cd) resistant and plant growth-promoting actinobacterial strains were isolated from metal-polluted soils and identified as Streptomyces sp. strain RA04 and Nocardiopsis sp. strain RA07. Multiple abiotic stress tolerances were found in these two actinobacterial strains, including Cd stress (CdS), drought stress (DS) and high-temperature stress (HTS). Both actinobacterial strains exhibited multifarious plant growth-promoting (PGP) traits such as phosphate solubilization, and production of indole-3-acetic acid, siderophores and 1-aminocyclopropane-1-carboxylate deaminase under CdS, DS and HTS conditions. The inoculation of strains RA04 and RA07 significantly increased Sorghum bicolor growth and photosynthetic pigments under CdS, DS, HTS, CdS + DS and CdS + HTS conditions as compared to their respective uninoculated plants. The actinobacterial inoculants reduced malondialdehyde concentration and enhanced antioxidant enzymes in plants cultivated under various abiotic stress conditions, indicating that actinobacterial inoculants reduced oxidative damage. Furthermore, strains RA04 and RA07 enhanced the accumulation of Cd in plant tissues and the translocation of Cd from root to shoot under CdS, CdS + DS and CdS + HTS treatments as compared to their respective uninoculated plants. These findings suggest that RA04 and RA07 strains could be effective bio-inoculants to accelerate phytoremediation of Cd polluted soil even in DS and HTS conditions.

Arsenic (As) contamination and bioaccumulation are a serious threat to agricultural plants. To address this issue, we checked the efficacy of As tolerant plant growth promoting bacteria (PGPB), zinc oxide nanoparticles (ZnO NPs) and oxalic acid (OA) in Luffa acutangula grown on As rich soil. The selected most As tolerant PGPB i.e Providencia vermicola exhibited plant growth promoting features i.e solubilzation of phosphate, potassium and siderophores production. Innovatively, we observed the synergistic effects of P. vermicola, ZnO NPs (10 ppm) and OA (100 ppm) in L. acutangula grown on As enriched soil (150 ppm). Our treatments both as alone and in combination alleviated As toxicity exhibited by better plant growth and metabolism. Results revealed significantly enhanced photosynthetic pigments, proline, relative water content, total sugars, proteins and indole acetic acid along with As amelioration in L. acutangula. Furthermore, upregulated plant resistance was manifested with marked reduction in the lipid peroxidation and electrolyte leakage and pronounced antagonism of As and zinc content in leaves under toxic conditions. These treatments also improved level of nutrients, abscisic acid and antioxidants to mitigate As toxicity. This marked improvement in plants’ defense mechanism of treated plants under As stress is confirmed by less damaged leaves cell structures observed through the scanning electron micrographs. We also found substantial decrease in the As bioaccumulation in the L. acutangula shoots and roots by 40 and 58% respectively under the co-application of P. vermicola, ZnO NPs and OA in comparison with control. Moreover, the better activity of soil phosphatase and invertase was assessed under the effect of our application. These results cast a new light on the application of P. vermicola, ZnO NPs and OA in both separate and combined form as a feasible and ecofriendly tool to alleviate As stress in L. acutangula.

This study aimed to identify the specific genes associated with plant growth promotion and cadmium tolerance in three bacteria strains associated with Pennisetum giganteum as well as to determine their biosafety levels in their potential use as biofertilizers for promoting plant growth and phytoremediation activities. The plant growth-promoting (PGP) abilities of Enterobacter cloacae strain RCB980 (A3), Klebsiella pneumonia strain kpa (A4), and Klebsiella sp. strain XT-2 (A7) were determined by a growth promotion trial and through testing for PGP traits such as 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase enzyme production, phosphorus solubilization, siderophore synthesis, and indole-3 acetic acid (IAA) production. The genes that potentially contribute to the beneficial activities of these three strains were identified through an analysis of their genomes. To establish the biosafety of the candidate PGPB, a pathological study was undertaken whereby 20 Kunming mice were injected intraperitoneally to study and analyze the effects of the strains on growth and lung paraffin sections of the mice. The strains had no obvious toxicity effect on the tested mice and were therefore not considered as highly virulent strains. These strains are thus considered non-toxic, safe, and highly recommended for use in environmental remediation strategies and agricultural production.

Heavy metal–resistant siderophore-producing bacteria (SPB) with plant growth–promoting traits can assist in phytoremediation of heavy metal–contaminated soil. We isolated siderophore-producing bacteria from Pb and Zn mine soil in Shangyu, Zhejiang, China. The isolate with the highest siderophore production, strain SX9, was identified as Burkholderia sp. Burkholderia sp. SX9 produced catecholate-type siderophore, with the highest production at a pH range of 6.0 to 8.0, a temperature range of 20 to 30 °C and NaCl concentration below 2%. Siderophore production was highest without Fe³⁺ and became gradually lower with increasing Fe³⁺ concentration. Minimal inhibitory concentrations (MIC) of Pb²⁺, Zn²⁺, Cu²⁺, and Cd²⁺ were 4000, 22000, 5000, and 2000 μmol L⁻¹, respectively. The strain had a strong metal solubilization ability: the contents of Cu²⁺, Zn²⁺, and Cd²⁺ in the supernatant were 47.4%, 133.0%, and 35.4% higher, respectively, in strain SX9–inoculated cultures than in the not inoculated controls. The siderophore produced by strain SX9 could combine with Fe³⁺, Zn²⁺, and Cd²⁺ with good effectiveness. The plant growth–promoting traits of the strain included indole acetic acid (IAA) production, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, and phosphate solubilization capability. Compared to the uninoculated growth medium and SX9 culture supernatant, the germination rate of Lolium perenne seeds was higher when inoculated with strain SX9 culture. In the experiment of seed germination, adding bacterial culture or supernatant could alleviate the toxicity of heavy metals to L. perenne seed germination. Under Cu²⁺ and Zn²⁺ stress, strain SX9 promoted the germination rate. Taken together, Burkholderia sp. SX9 had properties beneficial in the microbial enhancement of phytoremediation of soil contaminated with heavy metals.

The accumulation of heavy metal in the soil is a serious concern for sustainable food production due to their toxic effects on plants and other living things. The strategies are required on urgent bases for the management of metal-contaminated soils. Thus, the microbes from the genus Pseudomonas were characterized for different traits and lead (Pb)-resistant ability and their effects were assessed on growth, photosynthesis, antioxidant capacity, and Pb uptake by dill (Anethum graveolens L.). Furthermore, soil basal respiration and induced respiration in soil were also assessed under microbes and Pb stress. Among the tested three strains, Pseudomonas P159 and P150 were more tolerant to Pb stress than Pseudomonas P10, whereas P159 showed the highest values for phosphorus (P), siderophore, auxin, and hydrogen cyanide production. The bacterial inoculation increased the plant shoot dry weights, carbohydrates, proline, and chlorophyll contents under Pb stress. The catalase (CAT) and peroxidase (POD) activities of the plants were higher in bacterial-treated plants than control. The bacterial inoculation decreased Pb concentration in plants, and the response varied with the type of microbes. The bacterial strains enhanced the soil basal and induced respiration than respective Pb treatments alone. Overall, Pseudomonas P159 is potentially suitable for the remediation of Pb-contaminated soils. Graphical abstract

The development of mixed microbial agents can reduce the use of pesticides and fertilizers in agriculture. However, most previous studies focused only on the overall effects of mixed microbial agents and ignored the interactions between bacteria in mixed systems. In this study, Bacillus subtilis SL-44 and Enterobacter cloacae Rs-2 were used to explore the interactions between two different functional plant growth-promoting rhizobacteria (PGPR). The plant growth-promotion properties and inhibition rate of Rhizoctonia solani were determined, and the mechanism of the interactions under single and co-culture conditions was elucidated via transcriptomics analysis under single and co-culture conditions. Results showed that the co-culture was not conducive to B. subtilis SL-44 growth. Furthermore, the differentially expressed genes related to B. subtilis SL-44 developmental process and cell differentiation were downregulated by 82.7% and 84.8% respectively. Moreover, among the properties, only siderophore production by the mixed culture was higher than that of single cultures because of the upregulation of the siderophore-related genes of B. subtilis SL-44. In addition, results revealed the altruistic relationship between the two strains, and the chemical and non-chemical signals of their interaction. This study provides unique insights into PGPR interactions and offers guidance for the development and application of mixed microbial agents.

Soil cadmium (Cd) contamination resulting from anthropogenic activity poses severe threats to food safety and human health. In this study, a pot experiment was performed to evaluate the possibility of using urease-producing bacterium UR21 and eggshell (ES) waste for improving the physiological characteristics and reducing Cd accumulation of pakchoi (Brassica chinensis L.) plants. UR21 has siderophore and IAA production ability. The application of UR21 and ES individually or in combination could improve the root and shoot length, and fresh and dry weight of pakchoi plants under Cd stress. In Cd + ES + UR21–treated plants, the dry weight of shoot and root were increased by 61.54% and 72.73%, respectively. The chlorophyll a, chlorophyll b, and carotenoid content were increased by 52.19%, 42.95%, and 95.56% in Cd + ES + UR21–treated plants. Meanwhile, the H₂O₂ and MDA content were decreased while the SOD and POD activity were increased, and an increase of soluble protein level in pakchoi plants was observed under Cd + ES + UR21 treatment. Importantly, eggshell and UR21 alone or in combination induced a decline of Cd content in pakchoi plants, especially that Cd + ES + UR21 treatment decreased Cd content in shoot and root by 26.96% and 42.91%, respectively. Meanwhile, the soil urease and sucrase activities were enhanced. Generally, the combined application of ureolytic bacteria UR21 and eggshell exhibited better effects than applied them individually in terms of alleviating Cd toxicity in pakchoi plants. Our findings may give a unique perspective for an eco-friendly and sustainable strategy to remediate heavy metal–polluted soils.