The contamination of environment with heavy metals has become a serious problem which can affect the human health. Three heavy metals (Zn, Cd and Pb) were determined in soil and plants for below and aboveground parts along landfill Demina center, located in the wilaya of Jijel, Algeria to evaluate their behavior and uptake by Ditrichia viscosa, Juncus effusus and Solanum nigrum. In our research we tried to study the capacity of these spontaneous plants to accumulate and to translocate heavy metals from soil to their tissues during three years. The heavy metals examined in the soils of the study area showed variations in concentrations, the study area may be practically unpolluted with Zn and Pb (CF; 0.45 and 0.98 successively) and very contaminated with Cd (CF; 8.53). According to the results obtained, the soil is uncontaminated with lead (Igeo=-0.60) and zinc (Igeo= -1.42) but it is heavily contaminated with cadmium (Igeo=2.5) along the study area. Overall the BCFS (bioconcentration factors) are superior to 1, for the all heavy metals and species. However, BCFs follow the following order; BCFZn>BCFPb>BCFCd for Ditrichia viscosa, the following order BCFPb>BCFZn>BCFCd for Juncus effuses and follow the following order; BCFZn>BCFCd>BCFPb for Solanum nigrum. The TFs (translocation factor) of the present study showed that Solanum nigrum can translocate the three of the metals into their aboveground parts.

It has been proposed that non-protein thiols and organic acids play a major role in cadmium phytoavailability and distribution in plants. In the Cd-hyperaccumulator Solanum nigrum and non-accumulator Solanum melongena, the role of these organic ligands in the accumulation and detoxification mechanisms of Cd are debated. In this study, we used X-ray absorption spectroscopy to investigate Cd speciation in these plants (roots, stem, leaves) and in the soils used for their culture to unravel the plants responses to Cd exposure. The results show that Cd in the 100 mg.kg-1 Cd-doped clayey loam soil is sorbed onto iron oxyhydroxides. In both S. nigrum and S. melongena, Cd in roots and fresh leaves is mainly bound to thiol ligands, with a small contribution of inorganic S ligands in S. nigrum leaves. We interpret the Cd binding to sulfur ligands as detoxification mechanisms, possibly involving the sequestration of Cd complexed with glutathione or phytochelatins in the plant vacuoles. In the stems, results show an increase binding of Cd to -O ligands (>50% for S. nigrum). We suggest that Cd is partly complexed by organic acids for transportation in the sap.

Earthworms and arbuscular mycorrhizal fungi (AMF) act synergistically in the rhizosphere and may increase host plant tolerance to Cd. However, mechanisms by which earthworm-AMF-plant partnerships counteract Cd phytotoxicity are unknown. Thus, we evaluated individual and interactive effects of these soil organisms on photosynthesis, antioxidant capacity, and essential nutrient uptake by Solanum nigrum, as well as on soil quality following Cd exposure (0–120 mg kg⁻¹). Decreases in biomass and photosynthetic activity, as well as nutrient imbalances were observed in Cd-stressed plants; however, the addition of AMF and earthworms reversed these effects. Cd exposure increased superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities, whereas inoculation with Rhizophagus intraradices decreased those. Soil enzymatic activity decreased by 15–60% with increasing Cd concentrations. However, Cd-mediated toxicity was partially reversed by soil organisms. Earthworms and AMF ameliorated soil quality based on soil enzyme activity. At 120 mg kg⁻¹ Cd, the urease, catalase, and acid phosphatase activities were 1.6-, 1.4-, and 1.2-fold higher, respectively, in soils co-incubated with earthworms and AMF than in uninoculated soil. Cd inhibited shoot Fe and Ca phytoaccumulation, whereas AMF and earthworms normalized the status of essential elements in plants. Cd detoxification by earthworm-AMF-S. nigrum symbiosis was manifested by increases in plant biomass accumulation (22–117%), chlorophyll content (17–63%), antioxidant levels (SOD 10–18%, POD 9–25%, total polyphenols 17–22%, flavonoids 15–29%, and glutathione 7–61%). It also ameliorated the photosynthetic capacity, and macro- and micronutrient statuses of plants; markedly reduced the levels of malondialdehyde (20–27%), superoxide anion (29–36%), and hydrogen peroxide (19–30%); and upregulated the transcription level of FeSOD. Thus, the combined action of earthworms and AMF feasibly enhances metal tolerance of hyperaccumulating plants and improves the quality of polluted soil.

Solanum nigrum L. is a Cd hyperaccumulator, but the potential for continuous remediation, or different planting methods have not been fully characterized. The potential for continuous phytoremediation of Cd-contaminated farmland soil (2.08 mg kg⁻¹ Cd) by 2 planting methods (flowering harvest twice a year and maturity harvest once a year) was studied in a 3-year pot experiment. The total Cd accumulation (ug plant⁻¹) of the 3-year flowering stage treatments was 26.3% higher than that of the maturity stage treatments, which was mainly due to that flowering harvest twice a year caused 65.5% increase of shoot biomass. Similarly, the Cd decreased concentration in soil and Cd removal rate in the flowering stage treatments were 29.2% and 27.9% higher than that in the maturity stage treatments, respectively. After 3 years of phytoremediation, the extractable Cd concentration in soil was reduced by 36.4% in the flowering stage treatments and by 27.6% in the maturity stage treatments, which also led to the same decreasing trend of Cd accumulation of S. nigrum. In conclusion, the study results have demonstrated that the planting mode of two harvests a year at the flowering stage seems to be a viable option to apply for continuous phytoremediation of Cd-contaminated farmland soil.

Field trials contribute practical information towards the development of phytoremediation strategies that cannot be provided by laboratory tests. We conducted field experiments utilizing the Cd hyperaccumulator plant Solanum nigrum L., on farmland contaminated with 1.91 mg kg⁻¹ Cd in the soil. Our study showed that S. nigrum has a relatively high biomass. Planting density had a significant effect on the plant biomass and thus on overall Cd accumulation. For double harvesting, an optimal cutting position influenced the amount of Cd extracted from soils. Double cropping was found to significantly increase the amount of Cd extracted by S. nigrum. Fertilizing had no significant effect on plant biomass or on the Cd remediation of the soil over the short-term period. Our study indicates that S. nigrum can accumulate Cd from soils where the concentrations are relatively low, and thus has application for use in decontamination of slightly to moderately Cd-contaminated soil.

Contamination of heavy metals is a serious threat, which causes threats to the environment. Our study aimed to determine the role of endophytic bacteria in Cd phytoremediation and heavy metal ATPase gene expression. Cadmium (Cd) resistant endophytic bacteria were isolated from Solanum nigrum on LB agar plates, contaminated with 0–30 mg/L Cd. The phosphate solubilization and indole-3-acetic acid (IAA) production of endophytes were estimated by growing them on Pikovskaya agar medium and GC-MS analysis, respectively. An experiment in a pot was performed to evaluate the effects of bacteria on rice plants contaminated with 5–25 mg/L of Cd. Expression of Cd response genes was quantified through qRT–PCR and Cd translocation from one part to another part of the plant was measured through the ICP. BLAST alignment of 16 S-rDNA gene sequences confirmed the bacterial isolates as Serratia sp. AI001 and Klebsiella sp. Strain AI002. Both strains tolerated Cd up to 25 mg/L and produced 27–30 μg/mL of IAA. Inoculation of AI001 and AI002 improved plant growth dynamics (i.e., plant length, biomass, chlorophyll contents), relieved electrolyte leakage, and improved reduced glutathione significantly (P < 0.05). The inoculation of AI001 and AI002 significantly (P < 0.05) induced the expression of heavy metal ATPase genes ie., “HMA2, HMA3, and HMA4” and Cd translocation compared to uninoculated plants. Both AI001 and AI002 exhibited very prominent plant-growth-promoting and Cd phytoremediation properties. The results revealed that isolates also contributed a lot to the expression of rice plant heavy metal ATPase genes and in the Cd translocation in the plant.

The isotopic fractionation could contribute to understanding the Cd accumulation mechanisms in plant species. However, there are few of systematical investigations with regards to the Cd isotope fractionation in hyperaccumulator plants. The Cd tolerant Ricinus communis and hyperaccumulator Solanum nigrum were cultivated in nutrient solutions with varying Cd and EDTA concentrations. Cd isotope ratios were determined in the solution, root, stem and leaf. The two investigated plants were systematically enriched in light isotopes relative to their solutions (Δ114/110Cdplant-solution = −0.64‰ to −0.29‰ for R. communis and −0.84‰ to −0.31‰ for S. nigrum). Cd isotopes were markedly fractionated among the plant tissues. For both plant species, an enrichment in light Cd isotopes from solution to root was noted, followed by a slight depletion in light Cd isotopes from root to shoot. Noticeably, the chelation process has caused lighter Cd isotope enrichment in the root of R. communis and S. nigrum. Further, the good fits between △114/110Cdroot-plant and ln Froot (or between △114/110Cdshoot-plant and ln Fshoot) indicate that Cd isotopic signatures can be used to study Cd transportation during the metabolic process of plants. This study suggests that knowledge of the Cd isotope ratios could also provide new tool for identifying the Cd-avoiding crop cultivars.

The application of biodegradable chelating agents to enhance phytoremediation is a low-cost and promising method to improve the remediation efficiency of heavy metal–contaminated soil. The effects of N, N-bis glutamic acid (GLDA) on the growth and heavy metal absorption of Solanum nigrum were studied by pot experiment. The addition of chelate on the 20th day after sowing can improve the bioavailability of cadmium (Cd) in the soil. The results showed that the addition of chelating agents effectively improved the migration rate of the target heavy metal Cd in the soil, and significantly increased the accumulation of heavy metal in the roots, stems, and leaves of plants. The results showed that compared with the control group, the chelating agent could increase the extraction rate of total Cd by 28.65–68.74%. The application of GLDA significantly increased the accumulation of Cd (20 mg kg⁻¹ and 40 mg kg⁻¹), reaching 24.28–40.30 and 25.71–33.16 μg of pot⁻¹ DW, respectively. At the same time, GLDA increased Cd stress by decreasing plant biomass, inhibiting photosynthetic pigment synthesis and increasing MDA levels. These results indicated that GLDA could improve the absorption of Cd by S. nigrum, which provided a new idea for its practical application in the remediation of Cd-contaminated soil.

Degradable chelating agent–assisted phytoextraction is a promising method for the remediation of Cd-contaminated agricultural soil. However, there are limited studies that have examined the effect of chelating agents on soil solutions and soil quality in alkaline soil. In this study, the effects of S,S-ethylenediamine disuccinic acid (EDDS) on the growth and phytoextraction of Solanum nigrum L. (S. nigrum) were studied using pot experiments. The influence of EDDS on the soil solutions, heavy metal contents, and soil enzyme activities was evaluated. EDDS application increased the height of S. nigrum by 7.25–29.25 cm and increased the biomass of stem and leaf by 4.26–14.95 and 1.14–10.78 g/pot, respectively. The Cd concentrations in the leaves and berries of S. nigrum were 1.21–2.17 and 1.7–9.47 times higher than that of the control, respectively, and the Cd extraction amount in the shoots of S. nigrum increased by 22.78–256.16 μg/pot after EDDS application. The chelation of EDDS on heavy metals reached a peak after 7 days of application, decreased gradually with the degradation of EDDS, and disappeared after 30 days of application. Soil pH, available metals, metal speciation, and soil urease were significantly related to the application time of EDDS. Importantly, EDDS application 45 days before S. nigrum harvest treatments decreased the available metal concentrations and improved soil pH and urease activity. However, when EDDS was applied 15 days before S. nigrum harvest, the available Cd and Pb concentrations significantly increased and caused additional Pb pollution. Considering the chelation and degradation effects, the environmental implication, and the cost of EDDS, the results of this study showed that one application of EDDS was better than two applications, a 45-day application before harvest was preferred to a 15-day application, and application of 1–3 mM EDDS 30–45 days before S. nigrum harvest was the most promising application method for the remediation of Cd-contaminated alkaline soil.