خيارات البحث
النتائج 1 - 10 من 58
Acidity of size-fractionated aerosol particles.
1990
Ludwig J. | Klemm O.
Use of partially immobilized membrane ATPase in heavy metals determination
1998
Nikezic, G. (Institut za nuklearne nauke Vinca, Beograd (Yugoslavia). Laboratorija za molekularnu biologiju i endokrinologiju) | Horvat, A. | Jovanovic, D. | Vasic, V. | Vujisic, Lj. | Nedeljkovic, N. | Radak, B. | Kopecni, M.
Previous studies clearly indicated that membrane enzymes - ATPases i.e. Na, K-ATPase and Mg-ATPase, responded to presence of various organic and inorganic pollutants. In this work effect of mercury and cadmium on these enzymes activities was investigated in synaptic plasma membranes partially immobilized on microliter plate. Comparing those activities with the control enzyme activities obtained with native and partially immobilized mambranes it was concluded that: a. both metals exerted a concentration-dependent inhibition of investigated enzymes, b. for partially immobilized membranes estimated half maximum inhibition (IC50) values for Na, K-ATPase were IC50 (Hg) = 0.9 micromol/cubic cm, IC50 (cd) = 35 micromol/cubic cm and for Mg - ATPase IC50 (Hg) = 3.5 micromol/cubic cm, IC50 (Cd) = 36 micromol/cubic cm; for native membranes IC50 for Na,K-ATPase were IC50 (Hg) =3.3 micromol/cubic cm, IC50 (Cd) = 2 micromol/cubic cm and for Mg ATPase IC50 (Hg) = 2.3 micromol/cubic cm, IC50 (Cd) = 0.2 mmol/cubic cm. Obtained results indicate avaibility of microtitar plates for partially immobilization of membranes with aim to form a new biosensor for heavy metals detection.
اظهر المزيد [+] اقل [-]Immobilization of azo-dyes for optical detection of metal ions in water: reaction with Pd(II) [palladium]
2001
Savic, J. | Vasic, V. (Institut za nuklearne nauke "Vinca", Beograd (Yugoslavia). Laboratorija za fizicku hemiju)
The reaction of Pd(II) with 1,8-dihydroxy-2-(pyrazol-5-ylazo)-naphthalen-3,6-disulphonic acid immobilized by physical sorption onto Dowex 1-X8 ion-exchange resin was investigated with the aim to develop the sorption-spectroscopic test method for the detection of low Pd(II) concentrations in water. The resin phase absorption spectra of the reagent and its Pd(II) complex were followed. The immobilized reagent has the spectral characteristics similar to those in the water and forms with Pd(II) 1:1 complex with the absorption maximum at 650 nm. Parameters, such as pH, wavelength and contact time have been optimized for a given amount of the sorbed reagent. The experimental conditions for the linear dependence of absorbance vs. Pd(II) concentration have been determined.
اظهر المزيد [+] اقل [-][Tracing of the source of nickel in groundwater in Roskilde and Storstroems County]
1997
Knudsen, C.
[Pesticide analyses in groundwater: State of the art]
1997
Hansen, N.
Protocols for evaluating the natural attenuation of fuel hydrocarbons and chlorinated aliphatic hydrocarbons in groundwater
1997
Wiedemeier, T.H.
Detection of environmental carcinogens-DNA
1995
Pfohl Leszkowicz, A. (Ecole Nationale Superieure Agronomique de Toulouse (France). Laboratoire de Toxicologie et Securite Alimentaire) | Guillemaut, G. | Masfaraud, J.F. | Rether, B. | Haguenoer, J.M.
[State of drilling control with special analysis in Funen]
1995
Olesen, H.
[Plant uptake of PAH's and the analytical methods for detection]
2000
Samsoee-Petersen, L. | Mortensen, G.K.
[Detection of degradation products using chemical and biological methods [intrinsic bioremediation]]
1997
Dyreborg, S.
