The study examined the individual and combined effects of potassium ferrate(VI) additions and freeze-thaw conditioning for the treatment and dewatering of sludge samples. The first part of the experiments, using primary sludge, compared potassium ferrate(VI) additions prior to freeze-thaw treatment (pretreatment) versus potassium ferrate(VI) additions following freeze-thaw treatment (posttreatment). A low dose (LD) of 1.0 g/L and a high dose (HD) of 10.0 g/L of potassium ferrate(VI) were evaluated along with a freezing temperature of −20 °C and freezing periods of 1, 8 and 15 days. Following the designated freezing period, the samples were removed from the freezer and thawed at room temperature for 12 h. The second part of the study, using anaerobically digested sludge, evaluated the effects of potassium ferrate(VI) pretreatment, using LD = 0.5 g/L and HD = 5.0 g/L, and used simulated drainage beds to separate meltwater from the sludge cake during the thawing period. The study demonstrated that stand-alone freeze-thaw can reduce faecal coliform by >3-log after being frozen for only 1 day, and pretreatment with potassium ferrate(VI) can be used to improve the effects of freeze-thaw on faecal coliform inactivation in sludge. Furthermore, the drainability of the sludge following freeze-thaw was not significantly deteriorated when potassium ferrate(VI) was added to the sludge prior to freezing, despite greater than fourfold increases in the concentrations of soluble proteins and soluble carbohydrates. The meltwater collected during the sludge thawing was approximately 85 % of the initial sludge volume. When 5 g/L of potassium ferrate(VI) was added to the sludge prior to freezing, the meltwater collected had <0.28 MPN/mL faecal coliform, the turbidity was <10 NTU and the pH was 9.1. Pretreatment with potassium ferrate(VI) also reduced the concentration of faecal coliform in the sludge cake, suggesting that freeze-thaw coupled with potassium ferrate(VI) additions can be used to stabilise sludge and reduce sludge volume.

Soil thawing can affect the turnover of soil carbon (C) and nitrogen (N) and their release into the atmosphere. However, little has been known about the release of C and N during the thawing of alpine soils in the Qinghai-Tibet Plateau. This study investigated the effects of soil thawing on the release of CO₂, CH₄, and N₂O from alpine peatland soils and alpine meadow soils through an indoor experiment and determined the changes in the dissolved organic C (DOC), dissolved organic N (DON), NO₃ ⁻-N, NH₄ ⁺-N, and NO₂ ⁻-N concentrations in the soils after soil thawing. The freeze–thaw treatments were performed by incubating the soil columns at mild (−5 °C) and severe (−15 °C) for 14 days, and then at 5 °C for 18 days. The control columns were incubated at 5 °C. During thawing, the cumulative CO₂ emissions from the severely frozen alpine peatland soils and alpine meadow soils were 36 and 85 % higher than those from the control soils, and the cumulative N₂O emissions were 3.9 and 5.8 times higher than those from the control soils. However, the thawing after mild freezing produced no significant effects. The two freezing temperatures significantly increased the release of CH₄ from the alpine peatland soils, but the thawing of the severely frozen soils reduced the CH₄ uptake of the alpine meadow soils by 27 %. After the severely frozen alpine peatland soils thawed, the concentrations of DOC, DON, NO₃ ⁻-N, NH₄ ⁺-N, and NO₂ ⁻-N increased significantly, but NO₂ ⁻-N showed no significant changes for the alpine meadow soils. After thawing with mild freezing, DOC in the alpine peatland soils and NH₄ ⁺-N, NO₂ ⁻-N, and DOC in the alpine meadow soils showed no significant changes. This study indicates that the potential for release of C and N from alpine soils during thawing periods strongly depends on the freezing temperature and soil types.

Seasonal freeze-thaw cycle (FTC) is one of the key processes that affect heavy metal behaviors in soil. However, previous studies are mainly focused on extreme FTC treatments which may exaggerate the real FTC effects in the field. This study aimed to compare the effects of different FTC conditions on the adsorption and desorption behaviors of Cd in the surface black soil. Different minimum freezing temperatures (− 2, − 5, and − 15 °C), FTC rates (1 and 20 °C h⁻¹), freezing lengths (2 and 24 h), and FTC frequencies (1, 3, and 9) were investigated. The thawing temperature was set at 5 °C. The amplitude for the FTC rate, length, and frequency experiments ranged from 5 to − 2 °C. Our results indicated that the adsorption amounts of Cd presented an order of − 2 °C > − 15 °C > − 5 °C and 24 h > 2 h for different FTC amplitude- and freezing length-treated soils, and the adsorption amounts decreased with increasing FTC rate and frequency. Soil maximum adsorption amount of Cd increased with the increases of FTC frequency, freezing length, and FTC rate, while it decreased with the decreases of freezing temperature. Soil Cd desorption ratio decreased with the increases of FTC frequency, freezing length, and TFC rate, and it increased with the increasing freezing temperature. Our results suggested that FTC conditions can significantly influence the adsorption and desorption behaviors of heavy metal in soil.

Due to climate change and intense anthropogenic activity, organisms from cold regions are often exposed to combined effects of temperature fluctuations and contaminants. In this investigation, we assessed the lipid, protein, and carbohydrate energy budgets; the energy available (Ea); consumed (Ec); and cellular energy allocation (CEA) of the freeze-tolerant Enchytraeus albidus, when exposed to sublethal concentrations of 4-nonylphenol (a lipophilic contaminant) for 7 days, followed by exposure to different temperature regimes (continuous 2 °C, continuous −4 °C, and daily freeze-thaw cycles (FTC) (2 to −4 °C) for additional 10 days. Results showed that a pre-exposure to 4-nonylphenol (4-NP) induced important changes in the worms’ energy budgets and CEA and increased mortality with most severe effects observed for the FTC events. For FTC, lipids were the most accumulated energy source, whereas during freezing (−4 °C), proteins were the most used. FTC caused the highest Ec, indicating the higher energy requirements for organisms when shifting between freezing and thawing events. This is also in line with the higher mortality observed in FTC compared to continuous −4 °C or 2 °C. Worms exposed to continuous freezing presented relatively stable and positive levels of Ea and low levels of Ec, possibly related with the decrease in metabolism.

Freezing-thawing and saline-alkaline are the major abiotic stress for the pasture in most high-latitude areas, which are serious threats to the yield of pasture. In this study, the osmotic adjustment substances, membrane lipid peroxidation, and antioxidant enzymes activities of rye (Secale cereale L., cv. Dongmu-70) seedlings under different treatments: CK (no treatment), SC (Na₂CO₃ treatment), FT (freezing-thawing treatment), and FT+SC (combined Na₂CO₃ and freezing-thawing treatments), were investigated. At the freezing stage, the content of MDA and proline, the activity of APX, SOD, and POD increased with the decrease of the temperature in the leaves of rye seedlings in FT and FT+SC treatments and reached the maximum value at − 5 °C. In addition, the content of protein and H₂O₂, CAT activity reached the maximum value at 0 °C; the damage is larger under low temperature stress at 0 °C and − 5 °C in rye seedling. At the thawing stage, the content of MDA and H₂O₂ in seedling leaves decreased in FT and FT + SC treatments. These results demonstrated that proline content and antioxidant enzymes activities could play an important role in protecting cytomembrane and scavenging ROS respectively in rye under alkaline salt stress and freezing-thawing stress. The result also indicated rye seedlings were subjected to a freezing-thawing stress which resulted in a reversible (recoverable) injury.

To understand the potential environmental influence of animal manure under freeze-thaw cycles, pig manure was used to conduct a simulation experiment to explore the effects of freeze-thaw cycles on heavy metal distribution and form transformation. Thirty cycles of freezing and thawing were performed alternately by freezing at − 18 ± 2 °C for 24 h and thawing at 20 ± 2 °C for 24 h. By a serial wet sieving procedure, manure samples were separated into different sizes of 1000, 250, 75, 38, and < 38 μm. Solid samples were collected from the dry matter at each stage of sieve; then the washing waters were collected as liquid samples accordingly. The concentrations of heavy metals in solid/liquid samples and their five forms were analyzed. It showed that the concentrations of heavy metals in the solid and liquid samples gradually increased because of organic matter degradation during freezing and thawing cycles. The distribution of heavy metals on particles of different sizes was also affected by the degradation and breakup of pig manure; the metals showed a tendency to aggregate in small particles (< 38 μm). Among them, the percentage of Cu and Zn on < 38 μm particles increased by 162.3% and 554.1%, respectively. After several freeze-thaw cycles, the concentrations of EXCH-X (metals of exchangeable form) increased significantly, those of CARB-X (carbonate-bound form) and Fe/Mn-X (Fe/Mn oxide-bound form) decreased accordingly. These form transformations may be largely influenced by the enhancement of dissolved organic matter (DOM) and the reduction of pH value. Therefore, frequent freeze-thaw cycles may promote the mobility and bioavailability of heavy metals in pig manure. The results are significant for understanding the pollution risk of pig manure in the freeze-thaw regions.

This study investigated the effects of the freezing and thawing that accompany the warming process on the composition of the soil organic matter in the dissolved and colloidal fractions. Temperate soil samples were incubated in a refrigerator at 2 °C for 4 weeks and compared with those frozen at −20 °C in the second week followed by thawing at 2 °C to study a freeze-thaw effect with minimal effect from the thawing temperature. The freeze-thaw group was compared with those incubated at 25 °C in the last week to investigate a warming effect after thawing. Thawing at 2 °C after freezing at −20 °C increased the dissolved organic carbon (DOC), but decreased colloidal Ca. The subsequent warming condition greatly increased both DOC and colloidal Ca. The colloidal organic carbon (COC) and dissolved Ca showed rather subtle changes in response to the freeze-thaw and warming treatments compared to the changes in DOC and colloidal Ca. The fluorescence excitation-emission matrix (EEM) and Fourier transformation-infrared spectrometry (FT-IR) results showed that the freeze-thaw and warming treatments gave the opposite effects on the compositions of dissolved humic-like substances, polysaccharides or silicates, and aliphatic alcohols. A principal component analysis (PCA) with the DOC, fluorescence EEM, and FT-IR spectra produced two principal components that successfully distinguished the effects of the freeze-thaw and warming treatments. Due to the contrasting effects of the freeze-thaw and warming treatments, the overall effects of freeze-thaw events in nature on the dissolved and colloidal soil organic matter could vary depending on the thawing temperature.