Researches had shown that silica nanoparticles (SiNPs) could reduce the quantity and quality of sperms. However, chronic effects of SiNPs have not been well addressed. In this study, mice spermatocyte cells (GC-2spd cells) were continuously exposed to SiNPs (5 μg/mL) for 30 passages and then the changes of microRNA (miRNA) profile and mRNA profile were detected. The function of miRNAs was verified by inhibitors to explore the regulation role of miRNAs in reproductive toxicity induced by SiNPs. The results showed that SiNPs induced cytotoxicity, and activated autophagy in GC-2spd cells. SiNPs led to a total of 1604 mRNAs (697 up-regulated and 907 down-regulated) and 15 miRNAs (6 up-regulated such as miRNA-138 and miRNA-494 and 9 down-regulated) with different expression in GC-2spd cells. The combined miRNA profile and mRNA profile showed that 415 mRNAs with different expression in 5 μg/mL SiNPs group were regulated by miRNA. Furthermore, our study demonstrated that SiNPs decreased the expressions of AKT mRNAs. Moreover, SiNPs had an activation effect on the AMPK/TSC/mTOR pathway. However, inhibitor of miRNA-494 could attenuate the expression levels of AMPK, TSC, LC3Ⅱ and alleviate the decreased of AKT, mTOR, p-mTOR induced by SiNPs. The above results suggested that the low-dose SiNPs exposure could promote autophagy by miRNA-494 targeting AKT, thereby activating AMPK/TSC/mTOR pathway in GC-2spd cells. MiRNA-494 is an important regulator of autophagy by targeting AKT, which provides new evidence for the male reproductive toxicity mechanism of SiNPs.

Growing production and consumption of pharmaceuticals is a global problem. Due to insufficient data on the concentration and distribution of pharmaceuticals in the marine environment, there are no appropriate legal regulations concerning their emission. In order to understand all aspects of the fate of pharmaceuticals in the marine environment and their effect on marine biota, it is necessary to find the most appropriate model organism for this purpose. This paper presents an overview of the ecotoxicological studies of pharmaceuticals, regarding the assessment of Mytilidae as suitable organisms for biomonitoring programs and toxicity tests. The use of mussels in the monitoring of pharmaceuticals allows the observation of changes in the concentration and distribution of these compounds. This in turn gives valuable information on the amount of pharmaceutical pollutants released into the environment in different areas. In this context, information necessary for the assessment of risks related to pharmaceuticals in the marine environment are provided based on what effective management procedures can be developed. However, the accumulation capacity of individual Mytilidae species, the bioavailability of pharmaceuticals and their biological effects should be further scrutinized.

75 paired TSP and PM2.5 samples were collected over four seasons on Huaniao Island (HNI), an island that lies downwind of continental pollutants emitted from mainland China to the East China Sea (ECS). These samples were analyzed for organic carbon (OC) and elemental carbon (EC), with a special focus on char-EC (char) and soot-EC (soot), to understand their sources, and the scale and extent of pollution and dry deposition over the coastal ECS. The results showed that char concentrations in PM2.5 and TSP averaged from 0.13 to 1.01 and 0.31–1.44 μg m−3; while for soot, they were from 0.03 to 0.21 and 0.16–0.56 μg m−3, respectively. 69.0% of the char and 36.4% of the soot were present in PM2.5. The char showed apparent seasonal variations, with highest concentrations in winter and lowest in summer; while soot displayed maximum concentrations in fall and minimum in summer. The char/soot ratios in PM2.5 averaged from 3.29 to 17.22; while for TSP, they were from 1.20 to 7.07. Both of the ratios in PM2.5 and TSP were highest in winter and lowest in fall. Comparisons of seasonal variations in OC/EC and char/soot ratios confirmed that char/soot may be a more effective indicator of carbonaceous aerosol source identification than OC/EC. Annual average atmospheric dry deposition fluxes of OC and EC into ECS were estimated to be 229 and 107 μg m−2 d−1, respectively, and their deposition fluxes significantly increased during episodes. It was estimated that the loadings of OC + EC and EC accounted for 1.3% and 4.1% of the total organic carbon and EC in ECS surface sediments, respectively, implying a relatively small contribution of OC and EC dry deposition to organic carbon burial. This finding also indicates a possibly more important contribution of wet deposition to organic carbon burial in sediments of ECS, and this factor should be considered for future study.

Stable isotopes ratios (‰) of Hydrogen (δ2H) and Oxygen (δ1⁸O) were used to trace the groundwater recharge mechanism and geochemistry of arsenic (As) contamination in groundwater from four selected sites (Larkana, Naudero, Ghari Khuda Buksh and Dokri) of Larkana district. The stable isotope values of δ2H and δ1⁸O range from 70.78‰ to −56.01‰ and from −10.92‰ to −7.35‰, relative to Vienna Standard for Mean Ocean Water (VSMOW) respectively, in all groundwater samples, thus indicating the recharge source of groundwater from high-salinity older water. The concentrations of As in all groundwater samples were ranged from 2 μg/L to 318 μg/L, with 67% of samples exhibited As levels exceeding than that of World Health Organization (WHO) permissible limit 10 μg/L and 42% of samples expressed the As level exceeding than that of the National Environmental Quality Standard (NEQS) 50 μg/L. The leaching and vertical mixing with return irrigation water are probably the main processes controlling the enrichment of As in groundwater of Larkana, Naudero, Ghari Khuda Buksh and Dokri. The weathering of minerals mostly controlled the overall groundwater chemistry; rock-water interactions and silicate weathering generated yielded solutions that were saturated in calcite and dolomite in two areas while halite dissolution is prominent with high As area.

Despite the endeavour to eradicate informal e-waste recycling, remediation of polluted sites is not mandatory in many developing countries and thus the hazard of pollutants remaining in soil is often overlooked. It is noteworthy that a majority of previous studies only analysed a few pollutants in e-waste to reflect the impact of informal e-waste recycling. However, the actual impact may have been largely underestimated since e-waste contains various groups of pollutants and the effect of some emerging pollutants in e-waste remains unexplored. Thus, this study examined the contamination of metals, PBDEs and AHFRs in the vicinity of an abandoned e-waste recycling site. The accumulation and translocation of these pollutants in rice plants cultivated at the nearby paddy field were measured to estimate the health risk through rice consumption. We revealed that the former e-waste burning site was still seriously contaminated with some metals (e.g. Sn, Sb and Ag, Igₑₒ > 5), PBDEs (Igₑₒ > 3) and AHFRs (Igₑₒ > 3), which can disperse to the nearby paddy field and stream. The rice plants can effectively absorb some metals (e.g. Mo, Cr and Mn, BCF > 1), but not PBDEs and AHFRs (BCF < 0.15), from soil and translocate them to the leaves. Alarmingly, the health risk through rice consumption was high primarily due to Sb and Sn (HQ > 20), whereas PBDEs and AHFRs had limited contribution (HQ < 0.08). Our results imply that abandoned e-waste recycling sites still act as the pollution source, jeopardising the surrounding environment and human health. Since some trace metals (e.g. Sb and Sn) are seldom monitored, the impact of informal e-waste recycling would be more notorious than previously thought. Remediation work should be conducted promptly in abandoned e-waste recycling sites to protect the environment and human health.

A Fenton like advanced oxidation process (AOP) employing scrap zerovalent iron (SZVI) and hydrogen peroxide (H2O2) was studied for industrial textile wastewater treatment from a textile manufacturing plant located at Medellín, Colombia (South America). The wastewater effluent studied contains a mixture of organic compounds resistant to conventional treatments. The effect of initial pH and SZVI concentration and H2O2 concentration were studied by a response surface methodology (RSM) Box-Behnken design of experiment (BBD). The combined SZVI/H2O2 process led to reductions of 95% color, 76% of chemical oxygen demand (COD) and 71% of total organic carbon (TOC) at optimal operating conditions of pH = 3, SZVI = 2000 mg/L and [H2O2] = 24.5 mM. Molecular weight distribution measurement (MWD), ultraviolet–visible (UV–Vis) spectroscopy, HPLC, biodegradability and toxicity were used to characterize the pollutants after the treatment process finding that the resulting effluent was polluted mostly by low molecular weight carboxylic acids. A remarkable biodegradability enhancement of the effluent was evidenced by a BOD5/COD ratio increase from 0.22 to 0.4; also, the SZVI/H2O2 process successfully reduced the toxicity from 60% to 20% of dead A. Salina crustaceans.

Seasonal and diurnal variations of carbonyl compounds were investigated in the ambient air of a mountainous city in China, from September 2014 to July 2015. The most abundant carbonyl compounds are formaldehyde, acetaldehyde and acetone, propionaldehyde and methacryladehyde (MACR), which were all measured in most samples. The average concentrations of formaldehyde, acetaldehyde, acetone, propionaldehyde and MACR in the atmosphere in Changsha were broken down into each season: 6.57, 3.29, 3.66, 0.67 and 0.54 μg/m³ respectively during Spring, 14.09, 8.28, 9.02, 1.28 and 0.6 μg/m³, respectively during Summer, 9.24, 5.48, 8.62, 0.73 and 0.62 μg/m³, respectively during Autumn, and 5.88, 4.84, 7.84, 0.87 and 0.26 μg/m³ respectively during Winter. And majority of the species had higher concentration during noon, showing photochemical oxidation and human activities played an important role in diurnal variation. The highest average C1/C2 (formaldehyde/acetaldehyde) ratio was observed in summer (2.10) compared to those (1.33–2.03) in other seasons, implying the photochemical activities had a positive effect on increasing the ratio of C1/C2. In this study, the monthly concentration of formaldehyde produced from isoprene accounts for 4.8%–39.1% of formaldehyde in ambient air. Strong correlation among some carbonyl compounds means that they came from the same sources. Photochemical reaction was the main source of carbonyl compounds in summer and vehicular exhaust (gasoline and diesel engines) in winter. Changsha is not a completely urbanized city and it is rich in vegetation of broadleaf evergreen shrubs. Both atmospheric photochemical reactions and anthropogenic sources, including vehicular exhaust and industrial processes, dominate the levels of carbonyls. The ILTCR and HQ values of formaldehyde and acetaldehyde are 1.23E-04 and 1.34E-05, 2.80E-01 and 1.86E-01, respectively.

Urban trees have been assumed to effectively clean air particulate matter (PM), while the inter-species differences are not yet well defined, especially the PM chemical composition. In this study, PM from leaf surface and wax layer of 3 evergreen tree species (Juniper: Juniperus rigida; Black pine: Pinus tabuliformis var. mukdeais; Spruce: Picea koraiensis) were used for finding differences in PM adsorption and its compositional traits (characterized by X-ray diffraction, X-ray photoelectron spectroscopy, Fourier transform infrared spectrum and Inductively coupled plasma-optical emission spectrometry). Possible improvement in PM removal was also evaluated by a detail whole city tree census and different scenarios of species adjustment data. We found that: 1) the amount of PM on juniper leaves was 5.73 g m−2, 2–2.5-fold higher than black pine and spruce (p < 0.05). Of them, 38.73%, 38.22%, and 23.11% were in the wax layer. 2) Compared with the explicit interspecies differences in PM quantity, more complex interspecies difference showed different patterns for different compositional traits. In general, leaf surface PM had higher O, Si, Al, Fe, N, Pb, Cu, Ni, Cr, and Cd, while the wax PM had higher C and Na contents (p < 0.05). 3) Association ordination found that the smaller leaf size, lower leaf water content, higher leaf area per unit mass, higher wax content, and larger stomatal openness aligned with the more PM adsorption by leaf, together with the higher amounts of CO stretching, O, Si, Al, N, heavy metals of Pb, Cu, Ni, Cr, and Cd in PM. 4) Compared with the other 2 species, increase of juniper percentage in urban forests is more effective for maximizing PM removal from air, accompanying more heavy metal removal but less crystalized minerals in PM. Our findings highlight that proper species configuration in urban afforestation could maximize the air PM removal capacity.

Urban green spaces have the potential to mitigate and regulate atmospheric pollution. However, existing studies have primarily focused on the adsorption effect of different plants on atmospheric particulate matter (PM), whereas the effect of green space on PM has not been adequately addressed. In this study, the effect of different urban green space structures and configurations on PM was investigated through the 3D computational fluid dynamics (CFD) model ENVI-met by treating the green space as a whole based on field monitoring, and at the same time, the regulatory effect of green space on PM was examined by integrating information about the forest stand, PM concentration, and meteorological factors. The results show that the green space primarily affected wind speed but had no significant effect on relative humidity, temperature, or wind direction (P > 0.05). The PM concentration was significantly positively correlated with the relative humidity (P < 0.01), significantly negatively correlated with temperature (P < 0.05), but not significantly correlated with wind speed and direction (P > 0.05). Comparison with the measured values reveals that the ENVI-met model well reflected the differences in PM concentrations between different green spaces and the effect of green space on PM. In different green space structures, the uniform-type structure performed rather poorly at purifying PM, the concave-shaped structure performed the best, and the purifying effectiveness of the incremental-type and convex-shaped structure of green space was higher in the rear region than in the front region; in contrast, the degressional-type green space structure was prone to cause aggregation of the PM in the middle region. Broadleaf and broadleaf mixed forests had a better purifying effectiveness on PM than did coniferous forests, mixed coniferous forests, and coniferous broadleaf mixed forests. The above results are of great significance for urban planning and maximizing the use of urban green space resources.

Humic-like substances (HULIS) account for a considerable fraction of water-soluble organic matter (WSOM) in ambient fine particulates (PM2.5) over the world. However, systemic studies regarding the chemical characteristics, sources and redox activity of HULIS are still limited. In this study, the mass concentration, optical properties, and reactive oxygen species (ROS)-generation potential of HULIS were investigated in PM2.5 samples collected in Hong Kong during 2011–2012, and they all showed higher levels on days under regional pollution than on days under long range transport (LRT) pollution and local emissions. Positive matrix factorization (PMF) analysis was conducted regarding the mass concentration and dithiothreitol (DTT) activity of HULIS. Four primary sources (i.e. marine vessels, industrial exhaust, biomass burning, and vehicle emissions), and two secondary sources (i.e. secondary organic aerosol formation and secondary sulfate) were identified. Most sources showed higher contributions to both the mass concentration and DTT activity of HULIS on regional days than on LRT and local days, except that marine vessels had a higher contribution on local days than the other two synoptic conditions. Secondary processes were the major contributor to HULIS (54.9%) throughout the year, followed by biomass burning (27.4%) and industrial exhaust (14.7%). As for the DTT activity of HULIS, biomass burning (62.9%) and secondary processes (25.4%) were found to be the top two contributors. Intrinsic ROS-generation potential of HULIS was also investigated by normalizing the DTT activity by HULIS mass in each source. HULIS from biomass burning were the most DTT-active, followed by marine vessels; while HULIS formed through secondary processes were the least DTT-active. For the optical properties of HULIS, multiple linear regression model was adopted to evaluate the contributions of various sources to the light absorbing ability of HULIS. Biomass burning was found to be the only source significantly associated with the light absorbing property of HULIS.