خيارات البحث
النتائج 1021 - 1030 من 5,151
Analysis of the relationships between environmental noise and urban morphology النص الكامل
2018
Han, Xiaopeng | Huang, Xin | Liang, Hong | Ma, Song | Gong, Jianya
Understanding the effects of urban morphology on urban environmental noise (UEN) at a regional scale is crucial for creating a pleasant urban acoustic environment. This study seeks to investigate how the urban morphology influences the UEN in the Shenzhen metropolitan region of China, by employing remote sensing and geographic information data. The UEN in this study consists of not only regional environmental noise (RN), but also traffic noise (TN). The experimental results reveal the following findings: 1) RN is positively correlated with the nighttime light intensity (NTL) and land surface temperature (LST) (p < 0.05). More interestingly, landscape composition and configuration can also significantly affect RN. For instance, urban vegetation can mitigate the RN (r = −0.411, p < 0.01). There is a reduced RN effect when fewer buildings exist in an urban landscape, in terms of the positive relationship between building density and RN (r = 0.188, p < 0.01). Given the same percentage of building area, buildings are more effective at reducing noise when they are distributed across the urban scenes, rather than being spatially concentrated (r = −0.205, p < 0.01). 2) TN positively relates to large (r = 0.520, p < 0.01) and small–medium (r = 0.508, p < 0.01) vehicle flow. In addition, vegetation along or near roads can alleviate the TN effect (r = −0.342, p < 0.01). TN can also become more severe in urban landscapes where there is higher road density (r = 0.307, p < 0.01). 3) Concerning the urban functional zones, traffic land is the greatest contributor to urban RN, followed by mixed residential and commercial land. The findings revealed by this research will indicate how to mitigate UEN.
اظهر المزيد [+] اقل [-]Pristine graphene induces cardiovascular defects in zebrafish (Danio rerio) embryogenesis النص الكامل
2018
Manjunatha, Bangeppagari | Pak, Sŏng-ho | Kim, Kiwoong | Kundapur, Rajesh R. | Lee, Sang Joon
The multiple effect of pristine graphene (pG) toxicity on cardiovascular developmental defects was assessed using zebrafish as a model. Recently, the nanotoxicity is emerging as a critical issue, and it is more significant in embryogenesis. Especially, graphene and its derivatives have attracted a lot of interest in biomedical applications. However, very little is known about the toxic effects of pG which has been widely used carbon nanomaterial according to concentration and its effects on biological and cardiovascular development. In the present study, we examined the development of zebrafish embryos by exposing to pG (5, 10, 15, 20 and 25 μg/L) under different developmental toxicity end-points such as cardiotoxicity, cardiovascular defect, retardation of cardiac looping, apoptosis and globin expression analysis. For this, the developmental cardiotoxicity of pG at different concentrations and the specific cardiovascular defects thereof were elucidated for the first time. As a result, the exposure to pG was found to be a potential risk factor to cardiovascular system of zebrafish embryos. However, a further study on the variations of physical, molecular properties and mechanisms of nanotoxicity which vary depending on production method and surface functionalization is required. In addition, the potential risks of pG flakes to aquatic organisms and human health should be considered or checked before releasing them to the environment.
اظهر المزيد [+] اقل [-]Combined toxicity of organophosphate flame retardants and cadmium to Corbicula fluminea in aquatic sediments النص الكامل
2018
Li, Dandan | Wang, Peifang | Wang, Chao | Fan, Xiulei | Wang, Xun | Hu, Bin
Organophosphate flame retardants (OPFRs), as alternatives to polybrominated biphenyl ethers (PBDEs), are frequently detected in various environmental matrices. Owing to urbanization and industrial pollution, co-contamination of OPFRs and heavy metals is ubiquitous in the environment. The toxicity of OPFRs in aqueous phase is a significant concern, but uncertainty still exists regarding the co-toxicity to benthic organisms of OPFRs and metals in sediments. Hence, we explored the physiological response of Corbicula fluminea to OPFRs and Cd in sediments. The results indicated that the antioxidant system in the clams was stimulated in the presence of OPFRs and Cd, and the oxidative stress increased with increasing concentrations of OPFRs. In contrast, the cytochrome P450 (CYP450) content and acetylcholinesterase (AChE) activity were reduced by exposure to both OPFRs and Cd. The cytochrome P450 4 family (CYP4) mRNA expression and OPFR toxicity were lower than those in previously reported experiments conducted in the water phase. Moreover, the expression levels of metallothionein (MT) and AChE mRNA decreased when OPFRs and Cd were present together. The highest integrated biomarker response (IBR) index (IBR = 15.41) was observed in the presence of 45 mg kg⁻¹ Cd + 200 mg kg⁻¹ OPFRs, rather than the 45 mg kg⁻¹ Cd + 400 mg kg⁻¹ OPFRs treatment (IBR = 9.48). In addition, CYP450 and AChE in the digestive glands of C. fluminea exhibited significant correlations with the concentration of the OPFR/Cd mixture (p < 0.01) and could be effective biomarkers for OPFR and Cd co-contamination. The results potentially contribute to more realistic predictions and evaluations of the environmental risks posed by OPFRs in aquatic sediments contaminated with heavy metals, particularly with respect to the risk to benthic organisms.
اظهر المزيد [+] اقل [-]Dietary uptake, biodistribution, and depuration of microplastics in the freshwater diving beetle Cybister japonicus: Effects on predacious behavior النص الكامل
2018
Kim, Shin Woong | Kim, Dokyung | Chae, Yooeun | An, Youn-Joo
Microplastics (MPs) have adverse effects on aquatic organisms in marine environments; however, there is a lack of information on freshwater environments. This study investigated the dietary uptake, and biodistribution and depuration of MPs in the freshwater diving beetle Cybister japonicus (Coleoptera: Dytiscidae) after consumption of zebrafish (Danio rerio) exposed to MPs. The transfer of MPs in diving beetles after consumption of zebrafish was assessed to determine whether the presence of MPs affected diving beetle behavior and predation. We found that diving beetles that consumed MP-exposed fish had a significantly lower ingestion rate than the control. In addition, the trophic transfer rate of MPs was 13–18%. However, MPs were found only in the crop and proventriculus of the beetles, and all particles were depurated within 48 h, likely via regurgitation. As diving beetle is a top predator in freshwater ecosystems and could facilitate transfer from aquatic to terrestrial ecosystems via predation, its behavior towards indigestible MPs in its digestive organs (i.e., filtering and vomiting) could represent a meaningful phenomenon as a potential vector for MP transport. This is the first report of the trophic transfer of MPs from fish to dytiscid species, which helps clarify the effects and mechanisms of MPs in freshwater systems.
اظهر المزيد [+] اقل [-]Chronic exposure to microcystin-leucine-arginine promoted proliferation of prostate epithelial cells resulting in benign prostatic hyperplasia النص الكامل
2018
Pan, Chun | Chen, Yabing | Xu, Tianchi | Wang, Jing | Li, Dongmei | Han, Xiaodong
Microcystin-leucine-arginine (MC-LR), as a most common and deleterious variant among all structural analogues of Microcystins (MCs), can cause male reproductive dysfunction. However, its toxic effects on prostate in adult mice have not been invested in detail. In this study, we observed that MC-LR could enter prostate tissues and induce focal hyperplasia and prostate inflammation. Moreover, increased levels of prostate specific antigen (PSA) and prostate acid phosphatase (PAP) in serum of mice following chronic exposure to MC-LR were detected. We also examined increased expression of forkhead box protein M1 (FOXM1) and PSA in human prostate epithelial cells (RWPE-1) treated with MC-LR at low levels, and FOXM1 could regulate PSA expression. Furthermore, MC-LR also induced expression of CyclinD1 via FOXM1/Wnt/β-catenin signaling pathways in RWPE-1 cells, promoting proliferation of prostate epithelial cells, resulting in prostatic hyperplasia in vivo. As a foreign substance, MC-LR also induced immune reaction in RWPE-1 cells mediated by NF-κB pathway, promoting production of pro-inflammatory cytokines and chemokines. Collectively, these findings demonstrated that MC-LR may induce prostatic hyperplasia and prostatitis in mice following chronic low-dose exposure to MC-LR. This work may provide new perspectives in developing new diagnosis and treatment strategies for MC-LR-induced prostatic toxicity.
اظهر المزيد [+] اقل [-]Effects of physical characteristics of carbon black on metabolic regulation in mice النص الكامل
2018
Chuang, Hsiao-Chi | Hsiao, Ta-Chih | Lee, Chii-Hong | Chun-Te Lin, Justin | Chuang, Kai-Jen | Feng, Po-Hao | Cheng, Tsun-Jen
Potential adverse effects of human exposure to carbon black (CB) have been reported, but limited knowledge regarding CB-regulated metabolism is currently available. To evaluate how physical parameters of CB influence metabolism, we investigated CB and diesel exhaust particles (DEPs) and attempted to relate various physical parameters, including the hydrodynamic diameter, zeta potential, and particle number concentrations, to lung energy metabolism in female BALB/c mice. A body weight increase was arrested by 3 months of exposure to CB of smaller-size fractions, which was negatively correlated with pyruvate in plasma. There were no significant differences in cytotoxic lactate dehydrogenase (LDH) or total protein in bronchoalveolar lavage fluid (BALF) after 3 months of CB exposure. However, we observed alterations in acetyl CoA and the NADP/NADPH ratio in lung tissues with CB exposure. Additionally, the NADP/NADPH ratio was associated with the zeta potential of CB. Mild peribronchiovascular and interstitial inflammation and multinucleated giant cells (macrophages) with a transparent and rhomboid appearance and containing foreign bodies were observed in lung sections. We suggest that physical characteristics of CB, such as the zeta potential, may disrupt metabolism after pulmonary exposure. These results, therefore, provide the first evidence of a link between pulmonary exposure to CB and metabolism.
اظهر المزيد [+] اقل [-]Responses of soil organic carbon turnover to nitrogen deposition are associated with nitrogen input rates: Derived from soil 14C evidences النص الكامل
2018
Tan, Qiqi | Wang, Guoan | Liu, Xuejun | Hao, Tianxiang | Tan, Wenbing
Elevated atmospheric nitrogen (N) deposition has exerted profound influences on ecosystems. Understanding the effects of N deposition on the dynamics of soil organic carbon (SOC) is important in the studies of global carbon cycle. Although many studies have examined the effects of N deposition on SOC turnover using N addition experiments, the effects were reported to be different across studies. Thus, we lack a predictive understanding of how SOC turnover respond to atmospheric N deposition. The inconsistent results could be associated with ecosystem types and N addition rates. This study mainly wants to confirm the argument that the response of SOC turnover to N deposition is related with N input rates. We conducted a field experiment with multiple N addition levels (0, 3, 6, 12, and 24 g N m−2·yr−1) in Inner Mongolia Grassland, China. To better reveal the responses of SOC turnover to N enrichment, this study measured the soil 14C contents, because it can indicate SOC turnover directly. Compared with the control treatment (0 g N m−2·yr−1), N addition inhibits SOC turnover at the addition rate of 3 g N m−2·yr−1, whereas SOC turnover is not affected when N addition rate was 6, 12, and 24 g N m−2·yr−1. Our results suggest that N input rates affect the responses of SOC turnover to N enrichment. Thus, this study can confirm the argument mentioned above. Based on this study, it should be considered in the climate prediction model that varied atmospheric N deposition levels across regions may have different impacts on local SOC turnover. In addition, we also carried out a soil incubation to compare between the results obtained in incubation and that in 14C measurements. Two results are found to be inconsistent with each other. This indicates that soil respiration from incubation experiments could not comprehensively assess the effects of N deposition on SOC turnover.
اظهر المزيد [+] اقل [-]Arsenic mitigation in paddy soils by using microbial fuel cells النص الكامل
2018
Gustave, Williamson | Yuan, Zhao-Feng | Sekar, Raju | Chang, Hu-Cheng | Zhang, Jun | Wells, Mona | Ren, Yu-Xiang | Chen, Zheng
Arsenic (As) behavior in paddy soils couples with the redox process of iron (Fe) minerals. When soil is flooded, Fe oxides are transformed to soluble ferrous ions by accepting the electrons from Fe reducers. This process can significantly affect the fate of As in paddy fields. In this study, we show a novel technique to manipulate the Fe redox processes in paddy soils by deploying soil microbial fuel cells (sMFC). The results showed that the sMFC bioanode can significantly decrease the release of Fe and As into soil porewater. Iron and As contents around sMFC anode were 65.0% and 47.0% of the control respectively at day 50. The observed phenomenon would be explained by a competition for organic substrate between sMFC bioanode and the iron- and arsenic-reducing bacteria in the soils. In the vicinity of bioanode, organic matter removal efficiencies were 10.3% and 14.0% higher than the control for lost on ignition carbon and total organic carbon respectively. Sequencing of the 16S rRNA genes suggested that the influence of bioanodes on bulk soil bacterial community structure was minimal. Moreover, during the experiment a maximum current and power density of 0.31 mA and 12.0 mWm−2 were obtained, respectively. This study shows a novel way to limit the release of Fe and As in soils porewater and simultaneously generate electricity.
اظهر المزيد [+] اقل [-]Biotransformation of disperse dyes using nitroreductase immobilized on magnetic particles modified with tosyl group: Identification of products by LC-MS-MS and theoretical studies conducted with DNA النص الكامل
2018
Franco, Jefferson Honorio | Silva, Bianca F. da | de Castro, Alexandre A. | Ramalho, Teodorico C. | Pividori, María Isabel | Zanoni, Maria Valnice Boldrin
The present work evaluates the action of nitroreductase enzyme immobilized on Tosylactivated magnetic particles (MP-Tosyl) on three disperse dyes which contain nitro and azo groups. The dyes included Disperse Red 73 (DR 73), Disperse Red 78 (DR 78), and Disperse Red 167 (DR 167). The use of a magnet enabled the rapid and easy removal of the immobilized enzyme after biotransformation; this facilitated the identification of the products generated using high-performance liquid chromatography with diode array detector (HPLC-DAD) and mass spectrometry (LC-MS/MS). The main products formed by the in vitro biotransformation were identified as the product of nitro group reduction to the correspondent amine groups, which were denoted as follows: 50% of 2-(2-(4-((2-cyanoethyl)(ethyl)amino)phenyl)hydrazinyl)-5-nitrobenzonitrile, 98% of 3-((4-((4-amino-2-chlorophenyl) diazenyl)phenyl) (ethyl)amino)propanenitrile and 99% of (3-acetamido-4 - ((4-amino-2-chlorophenyl) diazenyl) phenyl) azanediyl) bis (ethane-2,1-diyl) for DR 73, DR 78 and DR 167, respectively. Based on the docking studies, the dyes investigated were found to be biotransformed by nitroreductase enzyme due to their favorable interaction with the active site of the enzyme. Theoretical results show that DR73 dye exhibits a relatively lower rate of degradation; this is attributed to the cyanide substituent which affects the electron density of the azo group. The docking studies also indicate that all the dyes presented significant reactivity towards DNA. However, Disperse Red 73 was found to exhibit a substantially higher reactivity compared to the other dyes; this implies that the dye possesses a relatively higher mutagenic power. The docking results also show that DR 73, DR 78 and DR 167 may be harmful to both humans and the environment, since the mutagenicity of nitro compounds is associated with the products formed during the reduction of nitro groups. These products can interact with biomolecules, including DNA, causing toxic and mutagenic effects.
اظهر المزيد [+] اقل [-]Stabilizing mixed fatty acid and phthalate ester monolayer on artificial seawater النص الكامل
2018
Li, Siyang | Du, Lin | Zhang, Qingzhu | Wang, Wenxing
Phthalate esters which are widely used as industrial chemicals have become widespread contaminants in the marine environment. However, little information is available on the interfacial behavior of phthalate esters in the seawater, where contaminants generally occur at elevated concentrations and have the potential to transfer into the atmosphere through wave breaking on sea surface. We used artificial seawater coated with fatty acids to simulate sea surface microlayer in a Langmuir trough. The interactions of saturated fatty acids (stearic acid (SA) and palmitic acid (PA)) with one of the most abundant phthalate esters (di-(2-ethylhexyl) phthalate (DEHP)), were investigated under artificial seawater and pure water conditions. Pure DEHP monolayer was not stable, while more stable mixed monolayers were formed by SA and DEHP on the artificial seawater at relatively low surface pressure. Sea salts in the subphase can lower the excess Gibbs free energy to form more stable mixed monolayer. Among the ten components in the sea salts, Ca²⁺ ions played the major role in condensation of mixed monolayer. The condensed characteristic of the mixed SA (or PA)/DEHP monolayers suggested that the hydrocarbon chains were ordered on artificial seawater. By means of infrared reflection-absorption spectroscopy (IRRAS), we found that multiple sea salt mixtures induced deprotonated forms of fatty acids at the air–water interface. Sea salts can improve the stability and lifetime of mixed fatty acid and phthalate ester monolayer on aqueous droplets in the atmosphere. Interfacial properties of mixed fatty acid and phthalate ester monolayers at the air–ocean interface are important to help understand their behavior and fate in the marine environment.
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