Sulfidized nanoscale zero-valent iron (S-nZVI) is a promising material for in situ soil remediation. However, its transformation (i.e., aging) and effects on the microbial community in soil ecosystems are largely unknown. In this study, S-nZVI having low (S-nZVI (L)) and high sulfur-doping (S-nZVI (H)) were incubated in soil microcosms and bare nZVI was used as a control. Their aged products were characterized using microspectroscopic analyses and the changes in the corresponding soil microbial community were determined using high-throughput sequencing analyses. The results indicate that severe corrosion of both bare and S-nZVI occurred over 56 days of aging with significant morphological and mineral changes. Magnetite, lepidocrocite, and goethite were detected as the main aged products. In addition, sulfate ions, pyrite, and iron polysulfide were formed in the aged products of S-nZVI. Cr(VI) removal test results indicated that S-nZVI(L) achieved the best results after aging, likely because of the optimal FeS arrangement on its nanoparticle surfaces. The presence of nZVI and S-nZVI increased the abundance of some magnetotactic microorganisms and altered bacterial and fungal community structures and compositions. Moreover, the addition of S-nZVI enriched some bacterial and fungal genera related to sulfur cycling because of the presence of sulfide-bearing material. The findings reveal the transformation of S-nZVI during aging and its effects on microbial communities in soil ecosystems, thereby helping to the evaluation of S-nZVI application in soil remediation.

Primitive electronic waste (e-waste) dismantling activities have been shown to be an important emissions source for a variety of toxic organic compounds, including carcinogenic polycyclic aromatic hydrocarbons (PAHs). Previous studies have found that some nitrated PAHs (NPAHs) are more toxic than their parent PAHs, however, little attention has been paid to the formation of PAH derivatives during e-waste processing and there is a lack of comprehensive data from field observations. In this study, the spatial distribution, temporal trends and atmospheric fate of NPAHs and hydroxylated PAHs (OH-PAHs) were investigated at typical e-waste dismantling sites, with monitoring data collected over three consecutive years. Compared to background levels, higher levels of NPAHs and OH-PAHs were found in air samples from an e-waste dismantling industrial park, with their seasonal and annual changes shown to be affected by e-waste dismantling activities. Atmospheric PM₂.₅ particles were found to have high relative abundances of NPAHs (76.9%–95.1%) and OH-PAHs (73.3%–91.6%), with particle-bound concentrations ranging from 20.1 to 88.8 and 37.1 to 107 pg m⁻³, respectively. The most abundant NPAH isomers were found to be 9-Nitroanthracene and 2-Nitrofluoranthene, while OH-PAH isomers containing 2–4 rings were predominant. Source identification was performed based on the specific diagnostic ratios of NPAH isomers, confirming that NPAH and OH-PAH emissions have multiple sources, including emissions related to the e-waste dismantling process, atmospheric photochemical reactions and traffic emissions. Further research on the fate of such derivatives and their potential use as markers for source identification, is urgently required.

Fluorinated pesticides acquired a significant market share in the agrochemical sector due to the surge of new fluoroorganic ingredients approved in the last two decades. This growing trend has not been accompanied by a comprehensive scientific and regulatory framework entailing all their potential negative impacts for the environment, especially when considering the hazardous properties that may result from the incorporation of fluorine into organic molecules. This review aims to address the safe/hazardous dichotomy associated with fluorinated pesticides by providing an updated outlook on their relevancy in the agrochemical sector and how it leads to their role as environmental pollutants. Specifically, the environmental fate and distribution of these pesticides in the ecosystems is discussed, while also analysing their potential to act as toxic substances for non-target organisms.

Heavy metals and organic dyes are the major source of water pollution. Herein, a trifunctional β−cyclodextrin−ethylenediaminetetraacetic acid−chitosan (β−CD−EDTA−CS) polymer was synthesized using an easy and simple chemical route by the reaction of activated β−CD with CS through EDTA as a cross-linker (amidation reaction) for the removal of inorganic and organic pollutants from aqueous solution under different parameters such as pH, time effect, initial concentration, reusability, etc. The synthesized adsorbent was characterized using powder X-ray diffraction, Fourier transform infrared spectroscopy, field scanning electron microscopy, energy dispersive spectroscopy, Brunauer-Emmett-Teller (BET), thermogravimetric analyzer techniques to investigate their structural, functional, morphological, elemental compositions, surface area and thermal properties, respectively. Two types of heavy metals, i.e., mercury (Hg²⁺) and cadmium (Cd²⁺), and three organic dyes, i.e., methylene blue (MB), crystal violet (CV) and safranin O (SO) were chosen as inorganic and organic pollutants, respectively, to study the adsorption capacity of β-CD-EDTA-CS in aqueous solution. The β-CD-EDTA-CS shows monolayer adsorption capacity 346.30 ± 14.0 and 202.90 ± 13.90 mg g⁻¹ for Hg²⁺ and Cd²⁺, respectively, and a heterogeneous adsorption capacity 107.20 ± 5.70, 77.40 ± 5.30 and 55.30 ± 3.60 mg g⁻¹ for MB, CV and SO, respectively. Kinetics results followed pseudo-second order (PSO) kinetics behavior for both metal ions and dyes, and higher rate constants values (0.00161–0.00368 g mg⁻¹ min⁻¹) for dyes confirmed the cavitation of organic dyes (physisorption). In addition, we have also demonstrated the performance of β-CD-EDTA-CS for the of four heavy metals Hg²⁺, Cd²⁺, Ni²⁺, and Cu²⁺ and three dyes MB, CV, and SO in secondary treated wastewater. Findings of this study indicate that β-CD-EDTA-CS simple and essay to synthesize and can be use in wastewater treatment.

The subcellular partitioning approach provides useful information on the location of metals within cells and is often used on organisms with high levels of bioaccumulation to establish relationships between the internal concentration and the potential toxicity of metals. Relatively little is known about the subcellular partitioning of metals in wild fish with low bioaccumulation levels in comparison with those from higher contaminated areas. This study aims to examine the subcellular partitioning of various metals considering their chemical affinity and essentiality at relatively low contamination levels. Class A (Y, Sr), class B (Cu, Cd, MeHg), and borderline (Fe, Mn) metal concentrations were measured in livers and subcellular fractions of yellow perch (n = 21) collected in Lake Saint-Pierre, QC, Canada. The results showed that all metals, apart from MeHg, were distributed among subcellular fractions according to their chemical affinity. More than 60% of Y, Sr, Fe, and Mn were found in the metal-sensitive fractions. Cd and Cu were largely associated with the metallothionein-like proteins and peptides (60% and 67% respectively) whereas MeHg was found mainly in the metal-sensitive fractions (86%). In addition, the difference between the subcellular distribution of Cu and other essential metals like Fe and Mn denotes that, although the essentiality of some metals is a determinant of their subcellular distribution, the chemical affinity of metals is also a key driver. The similarity of the subcellular partitioning results with previous studies on yellow perch and other fish species from higher contaminated areas supports the idea that metals are distributed in the cellular environment according to their chemical properties regardless of the bioaccumulation gradient.

The increasing use of rare earth elements (REEs) in electric and electronic equipment has been associated with the presence of these elements in aquatic systems. The present study aimed to evaluate the toxicity of two REEs, Lanthanum (La) and Gadolinium (Gd), towards the mussel species Mytilus galloprovincialis. For this, the toxicity was assessed after a short-term exposure (14 days) to an environmentally relevant concentration of each element (10 μg/L), followed by a recovery period (14 days) in the absence of any contaminant. The measured biomarkers included energy-related parameters, activity of antioxidant and biotransformation enzymes, indicators of oxidative damage, levels of oxidized glutathione and neurotoxicity. After exposure mussels accumulated more La (0.54 μg/g) than Gd (0.15 μg/g). After recovery higher concentration decrease was observed for Gd (≈40% loss) compared to La exposed mussels (≈30% loss) which may be associated with lower detoxification capacity of mussels previously exposed to La. Mussels increased their metabolism (i.e., higher electron transport system activity) only after the exposure to Gd. Exposure to La and Gd resulted into lower energy expenditure, while when both elements were removed glycogen and protein concentrations decreased to values observed in non-contaminated mussels. Antioxidant and biotransformation capacity was mainly increased in the presence of Gd. This defense response avoided the occurrence of cellular damage but still loss of redox balance was found regardless the contaminant, which was re-established after the recovery period. Neurotoxicity was only observed in the presence of Gd with no effects after the recovery period. Results showed that a short-term exposure to La and especially to Gd can exert deleterious effects that may compromise specific biochemical pathways in aquatic species, such as M. galloprovincialis, but under low concentrations organisms can be able to re-establish their biochemical status to control levels after a recovery period.

Plastic film mulch (PFM) is a double-edged-sword agricultural technology, which greatly improves global agricultural production but can also cause severe plastic pollution of the environment. Here, we characterized and quantified the amount of macro- and micro-plastics accumulated after 32 years of continuous plastic mulch film use in an agricultural field. An interactive field trial was established in 1987, where the effect of plastic mulching and N fertilization on maize yield was investigated. We assessed the abundance and type of macroplastics (>5 mm) at 0–20 cm soil depth and microplastic (<5 mm) at 0–100 cm depth. In the PFM plot, we found about 10 times more macroplastic particles in the fertilized plots than in the non-fertilized plots (6796 vs 653 pieces/m²), and the amount of film microplastics was about twice as abundant in the fertilized plots than in the non-fertilized plots (3.7 × 10⁶ vs 2.2 × 10⁶ particles/kg soil). These differences can be explained by entanglement of plastics with plant roots and stems, which made it more difficult to remove plastic film after harvest. Macroplastics consisted mainly of films, while microplastics consisted of films, fibers, and granules, with the films being identified as polyethylene originating from the plastic mulch films. Plastic mulch films contributed 33%–56% to the total microplastics in 0–100 cm depth. The total number of microplastics in the topsoil (0–10 cm) ranged as 7183–10,586 particles/kg, with an average of 8885 particles/kg. In the deep subsoil (80–100 cm) the plastic concentration ranged as 2268–3529 particles/kg, with an average of 2899 particles/kg. Long-term use of plastic mulch films caused considerable pollution of not only surface, but also subsurface soil. Migration of plastic to deeper soil layers makes removal and remediation more difficult, implying that the plastic pollution legacy will remain in soil for centuries.

The accumulation of human-derived debris in the oceans is a global concern and a serious threat to marine wildlife. There is a volume of evidence that points to deleterious effects of marine debris (MD) on cetaceans in terms of both entanglement and ingestion. This review suggests that about 68% of cetacean species are affected by interacting with MD with an increase in the number of species reported to have interacted with it over the past decades. Despite the growing body of evidence, there is an ongoing debate on the actual effects of plastics on cetaceans and, in particular, with reference to the ingestion of microplastics and their potential toxicological and pathogenic effects. Current knowledge suggests that the observed differences in the rate and nature of interactions with plastics are the result of substantial differences in species-specific diving and feeding strategies. Existing projections on the production, use and disposal of plastics suggest a further increase of marine plastic pollution. In this context, the contribution of the ongoing COVID-19 pandemic to marine plastic pollution appears to be substantial, with potentially serious consequences for marine life including cetaceans. Additionally, the COVID-19 pandemic offers an opportunity to investigate the direct links between industry, human behaviours and the effects of MD on cetaceans. This could help inform management, prevention efforts, describe knowledge gaps and guide advancements in research efforts. This review highlights the lack of assessments of population-level effects related to MD and suggests that these could be rather immediate for small populations already under pressure from other anthropogenic activities. Finally, we suggest that MD is not only a pollution, economic and social issue, but also a welfare concern for the species and populations involved.

Whether propylene oxide (PO) exposure is associated with hyperglycemia were rarely explored. We aimed to determine the relationship between PO exposure and glucose metabolism, and potential role of oxidative stress. Among 3294 Chinese urban adults, urinary PO metabolite (N-Acetyl-S-(2-hydroxypropyl)-L-cysteine, 2HPMA), biomarkers of oxidative DNA damage (8-oxo-7,8-dihydro-20-deoxyguanosine, 8-OHdG) and lipid peroxidation (8-isoprostane, 8-iso-PGF₂α) in urine were determined. The associations of 2HPMA with 8-OHdG, 8-iso-PGF₂α, fasting plasma glucose (FPG), and risk of diabetes were explored. The roles of 8-OHdG and 8-iso-PGF₂α on association of 2HPMA with FPG and risk of diabetes were detected. After adjusted for potential confounders, each 1-unit increase in log-transformed concentration of 2HPMA was associated with a 0.15-mmol/L increase in FPG level, and the adjusted OR (95% CI) of diabetes by the associations of log-transformed urinary 2HPMA concentrations was 1.47 (95% CI: 1.03–2.11). Combination effects of 2HPMA with 8-OHdG or 8-iso-PGF₂α on risk of diabetes were detected, and elevated 8-iso-PGF₂α significantly mediated 34.5% of the urinary 2HPMA-associated FPG elevation. PO exposure was positively associated with FPG levels and risk of diabetes. PO exposure combined with DNA oxidative damage or lipid peroxidation may increase the risk of diabetes, and lipid peroxidation may partially mediate the PO exposure-induced FPG elevation.

To decrease the concentration of the toxic metal cadmium (Cd) in topsoil, and the human food chain, many countries have limited the Cd concentration allowed in phosphorus (P) fertilisers. However, to inform those limits we need accurate estimates of Cd leaching from established farming systems. Different soil layers were sampled to 2000 mm depth of a long-term trial that had applied 22.5 kg P ha⁻¹ yr⁻¹ for 67 years to grazed pastures that received either no irrigation or were irrigated when soil moisture fell below 10 or 20%. The annual yield of Cd leaching from the top 150 mm of soil to the 151–250 mm layer was between 1.1 and 1.8 g ha⁻¹ with Cd leaching increasing with the frequency of irrigation. The rate of Cd accumulation measured to 2000 mm was within the mean and standard error estimated for treatments using a mass balance approach. Estimates of annual Cd leaching loss were like those established from field trials measuring leaching events over a year (0.3–1.8 g ha⁻¹) with a similar rate of P application (9–24 kg P ha⁻¹ yr⁻¹). Using a Cd leaching rate of 1.8 g ha⁻¹ yr⁻¹ and P applications rates of 22.5 kg P ha⁻¹, topsoil Cd concentrations may stop increasing if Cd concentrations in P fertiliser can be maintained at < 72 mg Cd kg⁻¹ P.