خيارات البحث
النتائج 1101 - 1110 من 8,010
The role of respiratory droplet physicochemistry in limiting and promoting the airborne transmission of human coronaviruses: A critical review النص الكامل
2021
Niazi, Sadegh | Groth, Robert | Spann, Kirsten | Johnson, Graham R.
Whether virulent human pathogenic coronaviruses (SARS-CoV, MERS-CoV, SARS-CoV-2) are effectively transmitted by aerosols remains contentious. Transmission modes of the novel coronavirus have become a hot topic of research with the importance of airborne transmission controversial due to the many factors that can influence virus transmission. Airborne transmission is an accepted potential route for the spread of some viral infections (measles, chickenpox); however, aerosol features and infectious inoculum vary from one respiratory virus to another. Infectious virus-laden aerosols can be produced by natural human respiratory activities, and their features are vital determinants for virus carriage and transmission. Physicochemical characteristics of infectious respiratory aerosols can influence the efficiency of virus transmission by droplets. This critical review identifies studies reporting instances of infected patients producing airborne human pathogenic coronaviruses, and evidence for the role of physical/chemical characteristics of human-generated droplets in altering embedded viruses’ viability. We also review studies evaluating these viruses in the air, field studies and available evidence about seasonality patterns. Ultimately the literature suggests that a proportion of virulent human coronaviruses can plausibly be transmitted via the air, even though this might vary in different conditions. Evidence exists for respirable-sized airborne droplet nuclei containing viral RNA, although this does not necessarily imply that the virus is transmittable, capable of replicating in a recipient host, or that inoculum is sufficient to initiate infection. However, evidence suggests that coronaviruses can survive in simulated droplet nuclei for a significant time (>24 h). Nevertheless, laboratory nebulized virus-laden aerosols might not accurately model the complexity of human carrier aerosols in studying airborne viral transport. In summary, there is disagreement on whether wild coronaviruses can be transmitted via an airborne path and display seasonal patterns. Further studies are therefore required to provide supporting evidence for the role of airborne transmission and assumed mechanisms underlying seasonality.
اظهر المزيد [+] اقل [-]Occurrence, influencing factors, toxicity, regulations, and abatement approaches for disinfection by-products in chlorinated drinking water: A comprehensive review النص الكامل
2021
Kali, Sundas | K̲h̲ān, Marīnah | Ghaffar, Muhammad Sheraz | Rasheed, Sajida | Waseem, Amir | Iqbal, Muhammad Mazhar | Bilal khan Niazi, Muhammad | Zafar, Mazhar Iqbal
Disinfection is considered as a vital step to ensure the supply of clean and safe drinking water. Various approaches are adopted for this purpose; however, chlorination is highly preferred all over the world. This method is opted owing to its several advantages. However, it leads to the formation of certain by-products. These chlorination disinfection by-products (DBPs) are genotoxic, carcinogenic and mutagenic. Still chlorination is being practiced worldwide. Present review gives insights into the occurrence, toxicity and factors affecting the formation of regulated (THMs, HAAs) and emerging DBPs (N-DBPs, HKs, HAs and aromatic DBPs) found in drinking water. Furthermore, remediation techniques used to control DBPs have also been summarized here. Key findings are: (i) concentration of regulated DBPs surpassed the permissible limit in most of the regions, (ii) high chlorine dose, high NOM, more reaction time (up to 3 h) and high temperature (up to 30 °C) enhance the formation of THMs and HAAs, (iii) high pH favors the formation of THMs while low pH is suitable of the formation of HAAs, (iv) high NOM, low temperature, low chlorine dose and moderate pH favors the formation of unstable DBPs (N-DBPs, HKs and HAs), (v) DBPs are toxic not only for humans but for aquatic fauna as well, (vi) membrane technologies, enhanced coagulation and AOPs remove NOM, (vii) adsorption, air stripping and other physical and chemical methods are post-formation approaches (viii) step-wise chlorination is assumed to be an efficient method to reduce DBPs formation without any treatment. Toxicity data revealed that N-DBPs are found to be more toxic than C-DBPs and aromatic DBPs than aliphatic DBPs. In majority of the studies, merely THMs and HAAs have been studied and USEPA has regulated just these two groups. Future studies should focus on emerging DBPs and provide information regarding their regulation.
اظهر المزيد [+] اقل [-]Effects of polystyrene nanoplastics on extracellular polymeric substance composition of activated sludge: The role of surface functional groups النص الكامل
2021
Qian, Jin | He, Xixian | Wang, Peifang | Xu, Bin | Li, Kun | Lu, Bianhe | Jin, Wen | Tang, Sijing
Here we investigated the acute effects (12 h exposure) of three polystyrene nanoplastics (PS NPs, including PS, PS−COOH and PS−NH₂) on extracellular polymeric substance (EPS) composition of activated sludge. Three PS NPs exhibited the significant inhibition in total EPS and protein (PRO) production. The functional groups involved in the interactions between PS NPs and EPS were C-(C, H), and those between PS-NH₂ NPs and EPS were CO and O–C–O. In addition, the dewaterability of activated sludge were optimized by three PS NPs, especially PS-NH₂ NPs. Three PS NPs caused nonnegligible cellular oxidative stress and cell membrane damage in activated sludge (PS NPs exposure concentration: 100 mg/L). Among them, the cell membrane damage caused by PS-NH₂ was the most significant. Overall, the degree of influence on EPS and cytotoxicity of activated sludge varies with the surface functional groups of PS NPs.
اظهر المزيد [+] اقل [-]Sorption of tetracycline onto hexabromocyclododecane/polystyrene composite and polystyrene microplastics: Statistical physics models, influencing factors, and interaction mechanisms النص الكامل
2021
Lin, Lüjian | Tang, Shuai | Wang, Xuesong | Sun, Xuan | Liu, Ying
Microplastics (MPs) are becoming a major concern due to their great potential to sorb and transport pollutants in the aquatic environment; hexabromocyclododecane (HBCD) is a common chemical additive in polystyrene (PS) MPs. However, the underlying mechanisms for the interaction of tetracycline (TC) onto HBCD-PS composites MPs (HBCD-PS MPs) are still not well documented. Our findings showed that the addition of HBCD resulted in a relatively higher hydrophobicity of PS MPs, and significantly enhanced the sorption ability of HBCD-PS MPs for TC. The kinetic models suggested that the sorption of TC onto PS and HBCD-PS MPs were mainly controlled by film diffusion and intra-particle diffusion, respectively. The statistical physics models were used to elucidate the sorption of TC onto PS and HBCD-PS MPs was associated with the formation of the monolayer, and the results indicated the TC was sorbed onto the two MPs by both multi-molecular and non-parallel processes. The TC sorption was solution pH-dependent while the effect of NaCl content on TC sorption was negligible. The presence of Cu(Ⅱ), Pb(Ⅱ), Cd(Ⅱ), and Zn(Ⅱ) ions had different influences on the TC sorption onto both the MPs. Overall, various mechanisms including π-π and hydrophobic interactions jointly regulated the sorption of TC onto both the MPs. Our results provided new insights into the sorption behavior and interaction mechanisms of TC onto both the MPs and highlighted that the addition of HBCD likely increased the enrichment capacity of MPs for pollutants in the environment.
اظهر المزيد [+] اقل [-]Organophosphate esters in surface soils from a heavily urbanized region of Eastern China: Occurrence, distribution, and ecological risk assessment النص الكامل
2021
Tang, Jianfeng | Sun, Jing | Ke, Ziyan | Yin, Hongling | Yang, Lei | Yen, Haw | Li, Xinhu | Xu, Yaoyang
Organophosphate esters (OPEs) pose increasing concerns for their widespread distribution in soil environments and potential threat to human health. In this study, we investigated the occurrence and associated risks of seven OPEs in surface soils and the potential influence of human activities on soil OPE contamination in a heavily urbanized region of the Yangtze River Delta in Eastern China. All target OPEs were detected in the soil samples (100% of samples) reflecting their widespread distribution in the study region. The total OPE concentration (the sum of the seven OPEs) ranged from 162.7 to 986.0 ng/g on a dry weight basis, with a mean value of 469.3 ± 178.6 ng/g. Tris (2-butoxyethyl) phosphate was the main compound, accounting for 67–78% of the total OPE concentration. Ecological risk assessment showed that tris(2-chloroisopropyl) phosphate, tris(2,3-dichloropropyl) phosphate, tris(2-butoxyethyl) phosphate, and tris(2-ethylhexyl) phosphate posed a medium potential risk to terrestrial biota (0.1 < risk quotient <1). The human exposure estimation showed insignificant risks to local population. Redundancy analysis revealed that the individual and total OPE contaminations were positively correlated with human activity parameters. The total OPE concentrations were positively correlated to population density (R² = 0.38, P < 0.001), and urban land use percentage (R² = 0.39, P < 0.001), while negatively correlated to forest land use percentage (R² = 0.59, P < 0.001), suggesting a significant contribution of human disturbance to OPE pollution. These results can facilitate OPE contamination control and promote sustainable soil management in urbanized and industrialized regions.
اظهر المزيد [+] اقل [-]Oxidative stress activates the Nrf2-mediated antioxidant response and P38 MAPK pathway: A possible apoptotic mechanism induced by BDE-47 in rainbow trout (Oncorhynchus mykiss) gonadal RTG-2 cells النص الكامل
2021
Zhou, Zhongyuan | Zhou, Bin | Chen, Hongmei | Lu, Keyu | Wang, You
Our previous study showed that 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47), the most biotoxic polybrominated diphenyl ether (PBDE) in the marine environment, induced apoptosis in rainbow trout gonadal RTG-2 cells. This effect occurred via ROS- and Ca²⁺-mediated apoptotic pathways, but the exact mechanism remains unknown. Therefore, in the present study, the possible mechanism was examined from the perspective of ROS-induced oxidative stress. The results showed that BDE-47 exposure significantly elevated the malondialdehyde (MDA) contents and the intracellular GSH/GSSG ratio, and the GSH-related enzymes were greatly altered, indicating alteration of the redox status and occurrence of oxidative stress. The mRNA levels of nuclear factor E2-related factor 2 (Nrf2) and its downstream genes were simultaneously greatly elevated. The p38 mitogen-activated protein kinase (MAPK) signaling pathway was also found to be induced by BDE-47 exposure. The addition of SB203580, a p38 MAPK inhibitor resulted in decreased apoptosis. In addition, supplementation with Ca²⁺ inhibitors BAPTA-AM positively affected p38 MAPK activation. Taken together, BDE-47 exposure resulted in the occurrence of oxidative stress and initiated the Nrf2-mediated antioxidant response. Subsequently, the altered redox status induced p38 MAPK activation, which played a pivotal role in the cellular apoptosis of RTG-2 cells.
اظهر المزيد [+] اقل [-]Glyphosate-based herbicide exposure affects diatom community development in natural biofilms النص الكامل
2021
Corrales, Natalie | Meerhoff, Mariana | Antoniades, Dermot
Glyphosate herbicide is ubiquitously used in agriculture and weed control. It has now been identified in aquatic ecosystems worldwide, where numerous studies have suggested that it may have both suppressive and stimulatory effects on diverse non-target organisms. We cultured natural biofilms from a hypereutrophic environment to test the effects on periphytic diatoms of exposure to a glyphosate-based herbicide formulation at concentrations from 0 to 10 mg L⁻¹ of active ingredient. There were clear and significant differences between treatments in diatom community structure after the 15-day experiments. Diversity increased more in low glyphosate treatments relative to higher concentrations, and compositional analyses indicated statistically significant differences between glyphosate treatments. The magnitude of change observed was significantly correlated with glyphosate-based herbicide concentration. Our results show that glyphosate-based herbicides have species-selective effects on benthic diatoms that may significantly alter trajectories of community development and therefore may affect benthic habitats and whole ecosystem function.
اظهر المزيد [+] اقل [-]tmbim4 protects against triclocarban-induced embryonic toxicity in zebrafish by regulating autophagy and apoptosis النص الكامل
2021
Hu, Zhiyong | He, Liting | Wei, Jiajing | Yufang, Su | Wang, Wei | Fan, Zunpan | Xu, Jia | Zhang, Yuan | Wang, Yongfeng | Peng, Meilin | Zhao, Kai | Zhang, Huiping | Liu, Chunyan
Triclocarban (TCC), an antibacterial agent widely used in personal care products, can affect embryonic development. However, the specific molecular mechanism of TCC-induced embryonic developmental damage remains unclear. In this study, TCC exposure was found to increase the expression of tmbim4 gene in zebrafish embryos. The tmbim4 mutant embryos are more susceptible to TCC exposure than wild-type (WT) embryos, with tmbim4 overexpression reducing TCC-induced embryonic death in the former. Exposure of tmbim4 mutant larvae to 400 μg/L TCC substantially increased apoptosis in the hindbrain and eyes. RNA-sequencing of WT and tmbim4 mutant larvae indicated that knockout of the tmbim4 gene in zebrafish affects the autophagy pathway. Abnormalities in autophagy can increase apoptosis and TCC exposure caused abnormal accumulation of autophagosomes in the hindbrain of tmbim4 mutant zebrafish embryos. Pretreatment of TCC-exposed tmbim4 mutant zebrafish embryos with autophagosome formation inhibitors, substantially reduced the mortality of embryos and apoptosis levels. These results indicate that defects in the tmbim4 gene can reduce zebrafish embryo resistance to TCC. Additionally, apoptosis induced by abnormal accumulation of autophagosomes is involved in this process.
اظهر المزيد [+] اقل [-]Artificial light reduces foraging opportunities in wild least horseshoe bats النص الكامل
2021
Luo, Bo | Xu, Rong | Li, Yunchun | Zhou, Wenyu | Wang, Weiwei | Gao, Huimin | Wang, Zhen | Deng, Yingchun | Liu, Ying | Feng, Jiang
Artificial light at night has been proposed as a global threat to biodiversity. Insectivorous bats are strictly nocturnal animals that are vulnerable to disruption from artificial light. Given that many light-sensitive bats tend to avoid night light during roost departure, it is often assumed that nighttime light pollution reduces their foraging opportunities, albeit empirical evidence in support of this hypothesis remains elusive. Here, we used least horseshoe bats, Rhinolophus pusillus, to assess whether white artificial light is detrimental for the opportunities of foraging. We manipulated the levels of ambient illumination and perceived predation risk inside the bat roost. We monitored bats' emergence activity using high-speed video and audio recording systems. DNA-based faecal dietary analysis and insect survey were applied to determine activity time of prey in foraging areas. Following experimentally manipulation of white light-emitting diode (LED) lighting 0–15 min after sunset, bat pass, flight duration, and echolocation pulse emission decreased. The mean emergence time of bats flying out was delayed by 14 min under lit treatment compared with the dark control. Only 10% of bats left for foraging during 40 min of light exposure. Aversive effects of LED light on bat emergence were robust regardless of the presence of a potential predator. Insect prey reached a peak of abundance between 30 and 60 min after sunset. These results demonstrate that white artificial light hinders evening emergence behavior in least horseshoe bats, leading to a mismatch between foraging onset and peak food availability. Our findings highlight that light pollution overrides foraging onset, suggesting the importance of improving artificial lighting scheme near the roosts of light-sensitive bats.
اظهر المزيد [+] اقل [-]Molecular mechanisms of zooplanktonic toxicity in the okadaic acid-producing dinoflagellate Prorocentrum lima النص الكامل
2021
Gong, Yufeng | Zhang, Keke | Geng, Ningbo | Wu, Minghuo | Yi, Xianliang | Liu, Renyan | Challis, Jonathan K. | Codling, Garry | Xu, Elvis Genbo | Giesy, John P.
Prorocentrum lima is a dinoflagellate that forms hazardous blooms and produces okadaic acid (OA), leading to adverse environmental consequences associated with the declines of zooplankton populations. However, little is known about the toxic effects and molecular mechanisms of P. lima or OA on zooplankton. Here, their toxic effects were investigated using the brine shrimp Artemia salina. Acute exposure of A. salina to P. lima resulted in lethality at concentrations 100-fold lower than densities observed during blooms. The first comprehensive results from global transcriptomic and metabolomic analyses in A. salina showed up-regulated mRNA expression of antioxidant enzymes and reduced non-enzyme antioxidants, indicating general detoxification responses to oxidative stress after exposure to P. lima. The significantly up-regulated mRNA expression of proteasome, spliceosome, and ribosome, as well as the increased fatty acid oxidation and oxidative phosphorylation suggested the proteolysis of damaged proteins and induction of energy expenditure. Exposure to OA increased catabolism of chitin, which may further disrupt the molting and reproduction activities of A. salina. Our data shed new insights on the molecular responses and toxicity mechanisms of A. salina to P. lima or OA. The simple zooplankton model integrated with omic methods provides a sensitive assessment approach for studying hazardous algae.
اظهر المزيد [+] اقل [-]