Session 3b: Heavy metals/N/POPs | International audience

"Best practices in the agrochemical industry covers the impact of agrochemicals in food and water supplies, and provides an overview of agrochemical residues and their health impacts as well as mitigation strategies. It includes extensive data tables covering USA and international regulatory frameworks for exposure to pesticides, including EPA, NIOSH, OSHA, WHO and ACGIH. Health impacts are explored, such as the pollution of drinking water with atrazine, trichloropropane and DBCP and the risks associated with these substances, including miscarriages and infertility. The authors also explore the effect of pesticide residues in food, including contaminants in organic food, and they present a case study concerning worker pesticide exposure. This book is a guide and professional reference for those involved in the agrochemical industry and it details safer manufacturing processes and procedures to limit pollution"--Page 4 of cover.

Annotation <div>Nucleic acids are the fundamental building blocks of life and are found in all living things. In recent years, their functions have been shown to extend beyond the Watson-Crick base pair recognition of complementary strands. Molecules (known as aptamers) consisting of 40-50 nucleotides have been isolated that are able to bind a broad range of molecules with high affinity and specificity. The molecules recognized by aptamers range from small organic molecules to proteins, cells and even intact viral particles. Catalytic DNA molecules called NAzymes (RNAzyme or DNAzyme) have also been shown to exist and, when combined with aptamers, are known as aptazymes. These biomolecules can be used to develop smart and innovative biosensors for environmental analysis. Monitoring of contaminants in the air, water and soil is a key component in understanding and managing risks to human health and ecosystems. This, in conjunction with the time and cost involved in traditional chemical analysis, means there is a growing need for simple, rapid, cost-effective and portable screening methods. Biosensors are compact devices which complement current field screening and monitoring methods. This book demonstrates the incredible opportunities that nucleic acids can offer to environmental analytical chemistry. The chapters: show how nucleic acids have a pivotal role in the development of smart biosensors for environmental monitoring; describe the development of biosensors based on aptamers and NAzymes for the detection of organic and inorganic pollutants; deal with the use of nucleic acid based biosensors for environmental toxicity screening, and detail the use of nanomaterials, as well as miniaturization and lab-on-a-chip technologies, for nucleic acid based biosensing systems.</div>

Understanding the ecological status of aquatic ecosystems and the impact of anthropogenic contamination requires correlating exposure to toxicants with impact on biological communities. Several tools exist for assessing the ecotoxicity of substances, but there is still a need for new tools that are ecologically relevant and easy to use. We have developed a protocol based on the substrate-induced respiration of a river biofilm community, using the MicroRes (TM) technique, in a pollution-induced community tolerance approach. The results show that MicroRes (TM) can be used in bioassays to assess the toxicity toward biofilm communities of a wide range of metals (Cu, Zn, Cd, Ag, Ni, Fe, Co, Al and As). Moreover, a community-level physiological profile based on the mineralization of different carbon substrates was established. Finally, the utility of MicroRes (TM) was confirmed in an in-situ study showing gradient of tolerance to copper correlated to a contamination gradient of this metal in a small river. A modified MicroRes (TM) technique as a tool for measuring induced tolerance to heavy metals of a microbial biofilm community.

Understanding the ecological status of aquatic ecosystems and the impact of anthropogenic contamination requires correlating exposure to toxicants with impact on biological communities. Several tools exist for assessing the ecotoxicity of substances, but there is still a need for new tools that are ecologically relevant and easy to use. We have developed a protocol based on the substrate-induced respiration of a river biofilm community, using the MicroRes (TM) technique, in a pollution-induced community tolerance approach. The results show that MicroRes (TM) can be used in bioassays to assess the toxicity toward biofilm communities of a wide range of metals (Cu, Zn, Cd, Ag, Ni, Fe, Co, Al and As). Moreover, a community-level physiological profile based on the mineralization of different carbon substrates was established. Finally, the utility of MicroRes (TM) was confirmed in an in-situ study showing gradient of tolerance to copper correlated to a contamination gradient of this metal in a small river. A modified MicroRes (TM) technique as a tool for measuring induced tolerance to heavy metals of a microbial biofilm community.

The effect of pH ranging from 8.0 to 6.8 (total scale - pHT) on fertilization, cleavage and larval development until pluteus stage was assessed in an intertidal temperate sea urchin. Gametes were obtained from adults collected in two contrasting tide pools, one showing a significant nocturnal pH decrease (lowest pHT = 7.4) and another where pH was more stable (lowest pHT = 7.8). The highest pHT at which significant effects on fertilization and cleavage were recorded was 7.6. On the contrary, larval development was only affected below pHT 7.4, a value equal or lower than that reported for several subtidal species. This suggests that sea urchins inhabiting stressful intertidal environments produce offspring that may better resist future ocean acidification. Moreover, at pHT 7.4, the fertilization rate of gametes whose progenitors came from the tide pool with higher pH decrease was significantly higher, indicating a possible acclimatization or adaptation of gametes to pH stress.

La chlordécone, insecticide organochloré de synthèse, était utilisée dans les bananeraies antillaises avant 1993. Pourtant, elle contamine encore les ressources en eau, certaines denrées, et des organismes aquatiques. Très tôt, la recherche agronomique s'est mobilisée pour répondre aux questions posées pour la gestion de cette crise : Où sont les sols pollués? Est-ce une pollution durable ? La molécule est peu mobile. Des cartes de risques, fondées sur leur occupation rétrospective en bananeraies, aboutissent à 1/5e de la SAU polluée en Guadeloupe, 2/5e en Martinique. Les sols riches en matière organique retiennent fortement la chlordécone. Elle ne se dégrade pas dans les sols aérés, seules les eaux de percolation peuvent la dissiper. Sa persistance est donc longue, d'un à quelques siècles selon les sols. La dépollution artificielle n'est pas actuellement opérationnelle. Il faut donc gérer cette pollution. Pour réduire l'exposition de la population et la contamination des denrées, les agriculteurs doivent disposer de systèmes de culture et d'élevage compatibles avec les niveaux de chlordécone des sols, restés fertiles. Un outil est disponible, il prend en compte le niveau de pollution de la parcelle et la contamination des cultures: certaines sont très contaminées (tubercules), d'autres indemnes (fruits d'arbres, banane, ananas, tomate,...). (Résumé d'auteur)

Lysosomal membrane stability, lipofuscin (LF), malondialdehyde (MDA), neutral lipid (NL) levels, as well as halogenated organic compounds (HOCs), Cr, Cd, Pb and Fe concentrations were analyzed in liver of black-legged kittiwake (BK), herring gull (HG), and northern fulmar (NF) chicks. There were significant species differences in the levels of NL, LF and lysosomal membrane stability. These parameters were not associated with the respective HOC concentrations. LF accumulation was associated with increasing Cr, Cd and Pb concentrations. HG presented the lowest lysosomal membrane stability and the highest. LF and NL levels, which indicated impaired lysosomes in HG compared to NF and BK. Lipid peroxidation was associated with HOC and Fe2+ levels. Specific HOCs showed positive and significant correlations with MDA levels in HG. The study indicates that contaminant exposure can affect lysosomal and lipid associated parameters in seabird chicks even at low exposure levels. These parameters may be suitable markers of contaminant induced stress in arctic seabirds.

We consider an optimal consumption and pollution problem that has two important features. Environmental damages due to economic activities may be irreversible and the level at which the degradation becomes irreversible is unknown. Particular attention is paid to the situation where agents are relatively impatient and/or do not care a lot about the environment and/or Nature regenerates at low rate. We show that the optimal policy of the uncertain problem drives the economy in the long run toward a steady state while, when ignoring irreversibility, the economy follows a balanced growth path accompanied by a perpetual decrease in environmental quality and consumption, both asymptotically converging toward zero. Therefore, accounting for the risk of irreversibility induces more conservative decisions regarding consumption and polluting emissions. In general, however, we cannot rule out situations where the economy will optimally follow an irreversible path and consequently, will also be left, in the long run, with an irreversibly degraded environment.