خيارات البحث
النتائج 1351 - 1360 من 7,997
Diazinon exposure produces histological damage, oxidative stress, immune disorders and gut microbiota dysbiosis in crucian carp (Carassius auratus gibelio) النص الكامل
2021
Tang, Jiali | Wang, Wenqian | Jiang, Yuanhe | Chu, Weihua
Diazinon is a common organophosphate pesticide widely used to control parasitic infections in agriculture. Excessive use of diazinon can have adverse effects on the environment and aquatic animal health. In the present study, the toxic effects of diazinon on the histology, antioxidant, innate immune and intestinal microbiota community composition of crucian carp (Carassius auratus gibelio) were investigated. The results showed that diazinon at the tested concentration (300 μg/L) induced gill and liver histopathological damages. Hepatic total superoxide dismutase (T-SOD), catalase (CAT), and glutathione S-transferase (GST) activities significantly decreased (P < 0.05) by 32.47%, 65.33% and 37.34%, respectively. However, the liver tissue malondialdehyde (MDA) content significantly (P < 0.05) increased by 138.83%. The 300 μg/L diazinon significantly (P < 0.05) downregulated the gene expression of TLR4, MyD88, NF-kB p100 and IL-8 but had no significant effect TNF-α (P = 0.8239). In addition, the results demonstrated that diazinon exposure could affect the intestinal microbiota composition and diversity. Taken together, the results of this study indicated that diazinon exposure can cause damage to crucian carp, induce histopathological damage in gill and liver tissues, oxidative stress in the liver, and innate immune disorders and alter intestinal microbiota composition and diversity.
اظهر المزيد [+] اقل [-]Understanding PFAAs exposure in a generalist seabird species breeding in the vicinity of a fluorochemical plant: Influence of maternal transfer and diet النص الكامل
2021
Lopez-Antia, Ana | Kavelaars, Marwa M. | Müller, Wendt | Bervoets, Lieven | Eens, Marcel
Perfluoroalkyl acids (PFAAs) are a focus of scientific and regulatory attention nowadays. However, PFAAs dynamics in the environment and the factors that determine wildlife exposure are still not well understood. In this study we examined PFAAs exposure in chicks of a generalist seabird species, the lesser black-backed gull (Larus fuscus), breeding 49 km away of a PFAAs hotspot (a fluorochemical plant in Antwerp, Belgium). In order to study the pathways of PFAAs exposure, we measured how chicks’ PFAAs burden varied with age, sex, and body condition. In addition, we related PFAA concentrations to chicks’ diet using stable isotope signatures. For this purpose, we studied plasma PFAA concentrations in 1-week and 4-week-old gull chicks. Only 4 (PFOS, PFOA, PFDA and PFNA) out of the 13 target PFAA compounds were detected. Measured concentrations of PFOS and PFOA were generally high compared to other seabird species but were highly variable between individuals. Furthermore, our results suggest that maternal transfer plays a significant role in determining chicks’ PFAAs burden, and that there are variable sources of exposure for PFOS and PFOA during post-hatching development. The association between PFOS and specific stable isotopes (i.e. δ¹⁵N and δ¹³C) suggests a higher exposure to PFOS in birds with a predominantly marine diet. We also found that males’ condition was positively associated with PFOS plasmatic concentration, probably due to the indirect effect of being fed a high quality (marine) diet which appears PFOS rich. Yet, exact exposure source(s) for PFOA remain(s) unclear. Given that PFOS concentrations measured in some chicks surpassed the toxicity reference value calculated for top avian predators, continued monitoring of exposure and health of this gull population, and other wildlife populations inhabiting the area, is highly recommended.
اظهر المزيد [+] اقل [-]Nutrient accumulation from excessive nutrient surplus caused by shifting from rice monoculture to rice–crayfish rotation النص الكامل
2021
Hou, Jun | Zhang, Dingyue | Zhu, Jianqiang
The potential environmental risk associated with nutrient surplus after switching from rice monoculture (RM) to rice–crayfish rotation (RCR) was assessed in the Jianghan Plains in China. Nutrient surplus was achieved by surveying 32 RM and 69 RCR and determining their nutrient inputs and outputs, and the soil nutrient status for different soil properties were recorded for 0–23 years. The annual average input of N, P₂O₅, and K₂O in RCR was 536, 185, and 253 kg ha⁻¹, respectively, wherein fertilizer and feed accounted for the major fraction of the total nutrient input. For instance, they accounted 58% and 18% of N, 74% and 24% of P₂O₅, and 70% and 30% of K₂O, respectively. The annual apparent surplus of N, P₂O₅, and K₂O was 397, 145, and 225 kg ha⁻¹, respectively, leading to low apparent nutrient use efficiency. Consequently, compared with RM, the total N and soil readily oxidized organic carbon in the upper soil surface (0–20 cm) for the RCR field significantly increased by 0.42–0.96 g kg⁻¹ and 1.63–3.19 g kg⁻¹, respectively. The available N, Olsen P, and exchangeable K of the RCR in the upper soil layer also increased significantly. In the RCR system, a significant positive linear relationship between the apparent accumulated nutrient surplus of N, P, and K elements and the total N, Olsen P, and exchangeable K present in the 0–60 cm soil profile was observed. In RCR, the soil pH in 0–60 cm soil profile and cation exchange capacity in the 0–20 cm soil layer increased as the cultivation time progressed. Nutrient accumulation in the soil not only enhanced soil fertility but also negatively influenced the environment. Therefore, several measures (e.g., new fertilization technologies, new fertilizer, legislation approaches for nutrient surplus, and technical training) should be adopted to control the nutrient surplus.
اظهر المزيد [+] اقل [-]Zearalenone and deoxynivalenol reduced Th1-mediated cellular immune response after Listeria monocytogenes infection by inhibiting CD4+ T cell activation and differentiation النص الكامل
2021
Cai, Guodong | Xia, Sugan | Zhong, Fang | Liu, Shuangshuang | Gu, Jianhong | Yuan, Yan | Zhu, Guoqiang | Zou, Hui | Liu, Zongping | Bian, Jianchun
Based on the fact that mycotoxins and the food-borne bacteria coexist in the natural environment and pose a significant health hazard to humans and animals, it is important to investigate the immunosuppressive mechanism of ZEA (zearalenone), DON (deoxynivalenol), and their combination in bacterial infections. In this study, we established a mouse model of mycotoxin low-dose exposure combined with Listeria monocytogenes infection and investigated the effects of ZEA, DON and their combination on Th1-mediated anti-intracellular bacterial infection based on CD4⁺ T cell activation and differentiation using both in vitro and in vivo analyses. The present study showed that both ZEA and DON aggravated Listeria monocytogenes infection in mice and affected the activation of CD4⁺ T cells and Th1 differentiation, including the effects on costimulatory molecules CD28 and CD152 and on cross-linking of IL-12 and IL-12R, by inhibiting T cell receptor (TCR) signaling. When compared with ZEA, DON was found to have a greater impact on many related indicators. Surprisingly, the combined effects of ZEA and DON did not appear to enhance toxicity compared to treatment with the individual mycotoxins. Our findings more clearly revealed that exposure to low-dose ZEA and DON caused immunosuppression in the body by mechanisms including inhibition of CD4⁺ T cells activation and reduction of Th1 cell differentiation, thus exacerbating infection of animals by Listeria monocytogenes.
اظهر المزيد [+] اقل [-]Integrated transcriptomics and proteomics revealed the distinct toxicological effects of multi-metal contamination on oysters النص الكامل
2021
Li, Yunlong | Wang, Wen-Xiong
The Pearl River Estuary (PRE) is the largest estuary in southern China and under high metal stress. In the present study, we employed an integrated method of transcriptomics and proteomics to investigate the ecotoxicological effects of trace metals on the Hong Kong oyster Crassostrea hongkongensis. Three oyster populations with distinct spatial distributions of metals were sampled, including the Control (Station QA, the lowest metal levels), the High Cd (Station JZ, the highest Cd), and the High Zn–Cu–Cr–Ni (Station LFS, with the highest levels of zinc, copper, chromium, and nickel). Dominant metals in oysters were differentiated by principal component analysis (PCA), and theirgene and protein profiles were studied using RNA-seq and iTRAQ techniques. Of the 2250 proteins identified at both protein and RNA levels, 70 proteins exhibited differential expressions in response to metal stress in oysters from the two contaminated stations. There were 8 proteins altered at both stations, with the potential effects on mitochondria and endoplasmic reticulum by Ag. The genotoxicity, including impaired DNA replication and transcription, was specifically observed in the High Cd oysters with the dominating influence of Cd. The structural components (cytoskeleton and chromosome-associated proteins) were impaired by the over-accumulated Cu, Zn, Cr, and Ni at Station LFS. However, enhanced tRNA biogenesis and exosome activity might help the oysters to alleviate the toxicities resulting from their exposure to these metals. Our study provided comprehensive information on the molecular changes in oysters at both protein and RNA levels in responding to multi-levels of trace metal stress.
اظهر المزيد [+] اقل [-]Where are we? Towards an understanding of the selective accumulation of microplastics in mussels النص الكامل
2021
Li, Jiana | Wang, Zhenglu | Rotchell, Jeanette M. | Shen, Xiaoteng | Li, Qipei | Zhu, Jingmin
Mussels are suggested as bioindicators of marine microplastic pollution. However, they are selective in regards to accumulation of microplastics. To make studies more targeted and comparable, ultimately helping to determine the suitability of the mussel as a bioindicator species for microplastic exposure, we review the published literature that has directly or indirectly demonstrated particle selection in mussels. The reported difference between microplastic levels in mussel tissues and environmental matrices provides evidence for their selective uptake characteristics. Both the organ-specific fate characteristics of microplastics, and the different movement patterns of microplastics in the same organ, show that selective translocation processes take place. The selective elimination is reflected in multiple aspects which include (1) the different characteristics of microplastics in excretion and mussel body; (2) the different retention time of various microplastics in mussels; and (3) the tissue-specific change in the numbers of microplastics during the depuration process. This selectivity is affected by the characteristics of the microplastics, the environmental, or laboratory exposure concentrations, feeding status, and other factors. There are still many research gaps and contradictory viewpoints in this field due to this complexity. The current methodology needs improvement and a breakthrough in standardization.
اظهر المزيد [+] اقل [-]Polycyclic aromatic compounds in the Canadian Environment: Aquatic and terrestrial environments النص الكامل
2021
Marvin, Christopher H. | Berthiaume, Alicia | Burniston, Deborah A. | Chibwe, Leah | Dove, Alice | Evans, Marlene | Hewitt, L Mark | Hodson, Peter V. | Muir, Derek C.G. | Parrott, Joanne | Thomas, Philippe J. | Tomy, Gregg T.
Polycyclic aromatic compounds (PACs) are ubiquitous across environmental media in Canada, including surface water, soil, sediment and snowpack. Information is presented according to pan-Canadian sources, and key geographical areas including the Great Lakes, the Alberta Oil Sands Region (AOSR) and the Canadian Arctic. Significant PAC releases result from exploitation of fossil fuels containing naturally-derived PACs, with anthropogenic sources related to production, upgrading and transport which also release alkylated PACs. Continued expansion of the oil and gas industry indicates contamination by PACs may increase. Monitoring networks should be expanded, and include petrogenic PACs in their analytical schema, particularly near fuel transportation routes. National-scale roll-ups of emission budgets may not expose important details for localized areas, and on local scales emissions can be substantial without significantly contributing to total Canadian emissions. Burning organic matter produces mainly parent or pyrogenic PACs, with forest fires and coal combustion to produce iron and steel being major sources of pyrogenic PACs in Canada. Another major source is the use of carbon electrodes at aluminum smelters in British Columbia and Quebec. Temporal trends in PAC levels across the Great Lakes basin have remained relatively consistent over the past four decades. Management actions to reduce PAC loadings have been countered by increased urbanization, vehicular emissions and areas of impervious surfaces. Major cities within the Great Lakes watershed act as diffuse sources of PACs, and result in coronas of contamination emanating from urban centres, highlighting the need for non-point source controls to reduce loadings.
اظهر المزيد [+] اقل [-]Intensive vegetable production results in high nitrate accumulation in deep soil profiles in China النص الكامل
2021
Bai, Xinlu | Jiang, Yun | Miao, Hongzhi | Xue, Shaoqi | Chen, Zhujun | Zhou, Jianbin
A comprehensive understanding of the patterns and controlling factors of nitrate accumulation in intensive vegetable production is essential to solve this problem. For the first time, the national patterns and controlling factors of nitrate accumulation in soil of vegetable systems in China were analysed by compiling 1262 observations from 117 published articles. The results revealed that the nitrate accumulation at 0–100 cm, 100–200 cm, 200–300 cm, and >300 cm were 504, 390, 349, and 244 kg N ha⁻¹, with accumulation rates of 62, 54, 19, and 16 kg N ha⁻¹ yr⁻¹ for plastic greenhouse vegetables (PG); for open field vegetables (OF), they were 264, 217, 228, and 242 kg N ha⁻¹ with accumulation rates of 26, 24, 18, and 10 kg N ha⁻¹ yr⁻¹, respectively. Nitrate accumulation at 0–100 cm, 0–200 cm, and 0–400 cm accounted for 5%, 11%, and 17% of accumulated nitrogen (N) inputs for PG, and represented 4%, 9%, and 13% of accumulated N inputs for OF. Nitrogen input rates and soil pH had positive effects and soil organic carbon, water input rate, and carbon to nitrogen ratio (C/N) had negative effects on nitrate accumulation in root zone (0–100 cm soil). Nitrate accumulation in deep vadose zone (>100 cm soil) was positively correlated with N and water input rates, and was negatively correlated with soil organic carbon, C/N, and the clay content. Thus, for a given vegetable soil with relatively stable soil pH and soil clay content, reducing N and water inputs, and increasing soil organic carbon and C/N are effective measures to control nitrate accumulation.
اظهر المزيد [+] اقل [-]Effects of mercury, organic carbon, and microbial inhibition on methylmercury cycling at the profundal sediment-water interface of a sulfate-rich hypereutrophic reservoir النص الكامل
2021
Fuhrmann, Byran C. | Beutel, Marc W. | O’Day, Peggy A. | Tran, Christian | Funk, Andrew | Brower, Sarah | Pasek, Jeffery | Seelos, Mark
Methylmercury (MeHg) produced by anaerobic bacteria in lakes and reservoirs, poses a threat to ecosystem and human health due to its ability to bioaccumulate in aquatic food webs. This study used 48-hr microcosm incubations of profundal sediment and bottom water from a sulfate-rich, hypereutrophic reservoir to assess seasonal patterns of MeHg cycling under various treatments. Treatments included addition of air, Hg(II), organic carbon, and microbial inhibitors. Both aeration and sodium molybdate, a sulfate-reducing bacteria (SRB) inhibitor, generally decreased MeHg concentration in microcosm water, likely by inhibiting SRB activity. The methanogenic inhibitor bromoethanesulfonate increased MeHg concentration 2- to 4- fold, suggesting that methanogens were potent demethylators. Pyruvate increased MeHg concentration under moderately reduced conditions, likely by stimulating SRB, but decreased it under highly reduced conditions, likely by stimulating methanogens. Acetate increased MeHg concentration, likely due to the stimulation of acetotrophic SRB. Results suggest that iron-reducing bacteria (IRB) were not especially prominent methylators and MeHg production at the sediment-water interface is elevated under moderately reduced conditions corresponding with SRB activity. In contrast, it is suppressed under oxic conditions due to low SRB activity, and under highly reduced conditions (<-100 mV) due to enhanced demethylation by methanogens.
اظهر المزيد [+] اقل [-]Urinary 3-phenoxybenzoic acid (3-PBA) concentration and pulmonary function in children: A National Health and Nutrition Examination Survey (NHANES) 2007–2012 analysis النص الكامل
2021
Hu, Peipei | Su, Weiwei | Vinturache, Angela | Gu, Haoxiang | Cai, Chen | Lu, Min | Ding, Guodong
Epidemiological studies have reported association of urinary 3-phenoxybenzoic acid (3-PBA), a major metabolite of pyrethroid insecticides (PYRs), with respiratory disease. However, knowledge regarding its effect on pulmonary function in susceptible children is limited. This study aimed to assess the associations between environmental 3-PBA concentrations and pulmonary function in children aged 6–17 years. Using data on 1174 children aged 6–17 years from the U.S. National Health and Nutrition Examination Survey (NHANES) 2007–2012, the exposure to PYRs was assessed by measuring urinary 3-PBA concentrations and pulmonary function was assessed by spirometry. Multivariable linear regression and generalized linear models (GLMs) were used to examine the associations between 3-PBA concentrations and pulmonary function in children, controlling for confounders. We found that 3-PBA concentrations were inversely associated with forced expiratory volume in 1 s (FEV₁), forced vital capacity (FVC), and peak expiratory flow (PEF) in the pediatric population (p-trends < 0.05). When stratified by age (6–10 and 11–17 years) and gender (boys and girls), the adverse effects of PYR exposures on pulmonary function were more pronounced among boys aged 11–17 years. Among this age group, 3-PBA concentrations were negatively associated with FEV₁, FVC, forced expiratory flow between 25% and 75% of FVC (FEF₂₅–₇₅%), and PEF. However, among children aged 6–10 years, no associations were found between 3-PBA concentrations and any of the pulmonary function measures, in either boys or girls. Our findings suggest that environmental PYR exposures may adversely affect children’s pulmonary function, with the strongest associations among 11–17 years old boys.
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