Carbonaceous constituents have various adverse impacts on human health, visibility, and climate change. Although comprehensive studies on the characteristics of carbonaceous constituents have been conducted recently, systematic studies covering both the mass characteristics and light-absorption properties of carbonaceous constituents on a regional scale in China are quite limited. In this study, current seasonal measurements of organic carbon (OC) and elemental carbon (EC) in PM₂.₅ were investigated during autumn and winter (1–30 October 2017 and December 18, 2017 to January 17, 2018) in six selected cities located at the eastern foot of the Taihang Mountains: Beijing, Baoding, Shijiazhuang, Handan, Xinxiang, and Zhengzhou. Seasonal variations were similar when Beijing was excluded. The lowest concentrations of OC (18.33 ± 9.39 μg/m³) and EC (7.66 ± 5.64 μg/m³) were observed in Xinxiang (autumn) and Beijing (winter), respectively, while the highest concentrations of OC (38.43 ± 62.10 μg/m³) and EC (12.24 ± 24.67 μg/m³) occurred in Baoding during winter mainly due to elevated fuel combustion for space heating. The results of the potential source contribution function (PSCF) analysis suggested that border zones between several provinces in North China should be highlighted in order to strengthen pollution control. Moreover, by separating the optical properties of brown carbon from those of black carbon, we were able to estimate the contributions of brown carbon to the PM₂.₅ total light-absorption coefficient. The results show that the brown carbon absorption coefficient (at 405 nm) in winter at six sites accounted for 21.2%, 33.3%, 34.7%, 39.1%, 48.6%, and 23.3% of the PM₂.₅ light absorption, which are values that are comparable to the contribution of black carbon in Xinxiang. These results provide a more comprehensive understanding of carbonaceous constituents on a regional scale.

Controlling ammonia (NH₃) emissions has been proposed as a strategy to mitigate haze pollution. To explore the role of NH₃ in haze pollution in Sichuan Basin, where agricultural activities are intense, hourly in situ data of NH₃, as well as nitric acid and secondary inorganic aerosols (SIAs) were gathered in Chengdu from April 2017 to March 2018. We found that NH₃ had an annual mean concentration of 9.7 ± 3.5 (mean ± standard deviation) μg m⁻³, and exhibited seasonal variations (spring > summer > autumn and winter) due to changes in emission sources and meteorological conditions (particularly temperature). Chengdu's atmosphere is generally NH₃-sufficient, especially in the warm seasons, implying that the formation of SIAs is more sensitive to the availability of nitric acid. However, an NH₃ “sufficient-to-deficient” transition was found to occur during winter pollution periods, and the frequency of NH₃ deficiency increased with the aggravation of pollution. Under NH₃-deficient conditions, the nitrogen oxidation ratio increased linearly with the increase in free NH₃, implying that NH₃ contributes appreciably to the formation of nitrate and thus to high PM₂.₅ loadings. No relationships of NH₃ with fossil fuel combustion–related pollutants were found. The NH₃ emissions from farmland and livestock waste in the suburbs of Chengdu and regional transport from west of Chengdu probably contribute to the occurrence of high PM₂.₅ loading in winter and spring, respectively. These results suggest that to achieve effective mitigation of PM₂.₅ in Chengdu, local and regional emission control of NH₃ and NOx synergistically would be effective.

Potential health benefits from improved ambient air quality during the COVID-19 shutdown have been recently reported and discussed. Despite the shutdown measures being in place, northern China still suffered severe haze episodes (HE) that are not yet fully understood, particularly how the source emissions changed. Thus, the meteorological conditions and source emissions in processing five HEs occurred in Beijing-Tianjin-Hebei area were investigated by analyzing a comprehensive real-time measurement dataset including air quality data, particle physics, optical properties, chemistry, aerosol lidar remote sensing, and meteorology. Three HEs recorded before the shutdown began were related to accumulated primary pollutants and secondary aerosol formation under unfavorable dispersion conditions. The common “business as usual” emissions from local primary sources in this highly polluted area exceeded the wintertime atmospheric diffusive capacity to disperse them. Thus, an intensive haze formed under these adverse meteorological conditions such as in the first HE, with coal combustion to be the predominant source. Positive responses to the shutdown measures were demonstrated by reduced contributions from traffic and dust during the final two HEs that overlapped the Spring and Lantern Festivals, respectively. Local meteorological dispersion during the Spring Festival was the poorest among the five HEs. Increased residential burning plus fireworks emissions contributed to the elevated PM₂.₅ with the potential of enhancing the HEs. Our results highlight that reductions from shutdown measures alone do not prevent the occurrence of HEs. To further reduce air pollution and thus improve public health, abatement strategies with an emphasis on residential burning are needed.

Exposure to environmental endocrine disrupting chemicals (EDCs) is highly suspected in prostate carcinogenesis. Though, estrogenicity is the most studied behavior of EDCs, the androgenic potential of most of the EDCs remains elusive. This study investigates the androgen mimicking potential of some common EDCs and their effect in androgen-dependent prostate cancer (LNCaP) cells. Based on the In silico interaction study, all the 8 EDCs tested were found to interact with androgen receptor with different binding energies. Further, the luciferase reporter activity confirmed the androgen mimicking potential of 4 EDCs namely benzo[a]pyrene, dichlorvos, genistein and β-endosulfan. Whereas, aldrin, malathion, tebuconazole and DDT were reported as antiandrogenic in luciferase reporter activity assay. Next, the nanomolar concentration of androgen mimicking EDCs (benzo[a]pyrene, dichlorvos, genistein and β-endosulfan) significantly enhanced the expression of AR protein and subsequent nuclear translocation in LNCaP cells. Our In silico studies further demonstrated that androgenic EDCs also bind with epigenetic regulatory enzymes namely DNMT1 and HDAC1. Moreover, exposure to these EDCs enhanced the protein expression of DNMT1 and HDAC1 in LNCaP cells. These observations suggest that EDCs may regulate proliferation in androgen sensitive LNCaP cells by acting as androgen mimicking ligands for AR signaling as well as by regulating epigenetic machinery. Both androgenic potential and epigenetic modulatory effects of EDCs may underlie the development and growth of prostate cancer.

As an emerging brominated flame retardant (BFR), tetrabromobisphenol S (TBBPS) has been frequently detected in the environmental media and organisms. Knowledges on the transformation and fate of TBBPS in both environment and engineering systems are essential to its ecological risk assessment. Herein, we reported the photochemical decomposition of TBBPS in aqueous solution upon 254 nm ultraviolet irradiation (UV₂₅₄). Results show that TBBPS was highly photoreactive, most likely due to the presence of four ortho-bromine substituents. The molar absorption coefficient and quantum yield of TBBPS were found to be pH-dependent, with the monoanionic form being most photoreactive. A series of photoproducts were identified by solid phase extraction (SPE) combined with liquid chromatography-electrospray ionization-triple quadrupole mass spectrometry (LC-ESI(+)-MS/MS. The photolysis of TBBPS likely proceeded through photonucleophilic substitution, photoreductive debromination, and β-scission reactions. A ketocarbene, possibly derived from the lower lying excited triplet state, was proposed to be involved in the photolysis of TBBPS. Ion chromatography analysis revealed that debromination occurred quickly, and the yield of bromide (Br⁻) approached 100% after 90 min irradiation. The presence of SRNOM and MRNOM inhibited the photodegradation rate of TBBPS, which is likely due to the light-screening and physical quenching effects of natural organic matter (NOM). Our results reveal that photolysis is an important process for the attenuation of TBBPS in aquatic system; however, naturally occurring species such as NOM can appreciably retard the decay of TBBPS.

Gala Lake is an internationally important lake due to its location on one of the world's most important bird migration routes. For this reason, water quality of the lake is of great concern. However, the lake surrounded by paddy fields may face eutrophication and toxic metal contamination due to excessive use of fertilizers and pesticides. In this study, impact of paddy fields on water quality of the Gala Lake was investigated. The concentrations of metal(loid)s and physico-chemical parameters in surface water samples taken from the lake were measured and compared with water quality guidelines. Also, human health risks and contamination status of metal(loid)s were assessed. The mean NO₂, SRP and BOD concentrations in the lake exceeded the permissible levels for both salmonid and cyprinid waters. The mean BOD value indicated contaminated water quality in the lake, while mean COD and SRP values indicated lightly contaminated water quality. The mean As, Cr and Pb values in the winter exceeded the drinking water limits set by WHO and EC, while the mean Cr and Zn values exceeded the limit values for the protection of freshwater aquatic organisms set by USEPA. Similarly, heavy metal pollution index and the degree of contamination values in the winter indicated that the lake water is moderately polluted. Health risk assessment results revealed that As and Cr in the lake water via ingestion exposure pathway may pose both non-carcinogenic and carcinogenic health risks to the residents. The results of this study indicated that paddy fields are a major source of nutrients, organic matter and toxic metal(loid)s to the Gala Lake. To improve the water quality of the lake, we suggest that excessive use of fertilizers and pesticides should be controlled to reduce metal(loid) and nutrient loads from the paddy fields.

To prevent the spread of the COVID-19 epidemic, the Chinese megacity Wuhan has taken emergent lockdown measures starting on January 23, 2020. This provided a natural experiment to investigate the response of air quality to such emission reductions. Here, we decoupled the influence of meteorological and non-meteorological factors on main air pollutants using generalized additive models (GAMs), driven by data from the China National Environmental Monitoring Center (CNEMC) network. During the lockdown period (Jan. 23 – Apr. 8, 2020), PM₂.₅, PM₁₀, NO₂, SO₂, and CO concentrations decreased significantly by 45 %, 49 %, 56 %, 39 %, and 18 % compared with the corresponding period in 2015–2019, with contributions by S(meteos) of 15 %, 17 %, 13 %, 10 %, and 6 %. This indicates an emission reduction of NOₓ at least 43 %. However, O₃ increased by 43 % with a contribution by S(meteos) of 6 %. In spite of the reduced volatile organic compound (VOC) emissions by 30 % during the strict lockdown period (Jan. 23 – Feb. 14, 2020), which likely reduced the production of O₃, O₃ concentrations increased due to a weakening of the titration effect of NO. Our results suggest that conventional emission reduction (NOₓ reduction only) measures may not be sufficient to reduce (or even lead to an increase of) surface O₃ concentrations, even if reaching the limit, and VOC-specific measures should also be taken.

Raphidiopsis raciborskii is a diazotrophic and potentially toxic cyanobacterium. To date, this species has successfully invaded many regions from the tropics to sub-tropical and temperate regions, typically forming blooms at temperatures greater than 25 °C. However, there have been a few cases in which R. raciborskii blooms have occurred at low temperatures (below 15 °C), but its cause and mechanisms remain unclear. In this study, field investigations revealed that R. raciborskii blooms occurred at 10–15 °C in Lake Xihu, Yunnan, China. The biomass of R. raciborskii was found to be positively related to nitrate concentrations in this lake. Three strains of R. raciborskii, two isolated from Lake Xihu (CHAB 6611 and CHAB 6612) and one from Lushui Reservoir in central China (CHAB 3409), were used for growth experiments at 15 °C. The three strains exhibited genotypic (16S rRNA and ITS-L genes) and physiological differences in response to nitrogen concentrations at low temperature. The growth rates of strains CHAB 6611 and CHAB 6612 increased with nitrogen concentration while CHAB 3409 could not grow at 15 °C. Furthermore, the growth and phenotypic responses of CHAB 6611 and CHAB 6612 to nitrogen concentrations were different, despite the closer genetic relationship shared by these two strains. Thus, increased nitrogen concentration in water may enhance the biological availability and utilization of nitrogen by R. raciborskii, which is the external promoter, leading to improving the resistance of R. raciborskii to low temperature. The internal cause is the presence of ecotypes in R. raciborskii populations with adaptation to low temperature. With increasing global eutrophication, the distribution range of R. raciborskii as well as the scale of its blooms will increase. As such, the risk of exposure of aquatic biota and humans to cylindrospermopsin is also expected to increase.

Water eutrophication caused by harmful algal blooms (HABs) occurs worldwide. It causes huge economic losses and has serious and potentially life-threatening effects on human health. In this study, the bacterium Raoultella sp. S1 with high algicidal efficiency against the harmful algae Microcystis aeruginosa was isolated from eutrophic water. The results showed that Raoultella sp. S1 initially flocculated the algae, causing the cells to sediment within 180 min and then secreted soluble algicidal substances that killed the algal cells completely within 72 h. The algicidal activity was stable across the temperature range −85.0 to 85.0 °C and across the pH range 3.00–11.00. Scanning electron microscopy (SEM) revealed the crumpling and fragmentation of cells algal cells during the flocculation and lysis stages. The antioxidant system was activated under conditions of oxidative stress, causing the increased antioxidant enzymes activities. Meanwhile, the oxidative stress response triggered by the algicidal substances markedly increased the malondialdehyde (MDA) and glutathione (GSH) content. We investigated the content of Chl-a and the relative expression levels of genes related to photosynthesis, verifying that the algicidal compounds attack the photosynthetic system by degrading the photosynthetic pigment and inhibiting the expression of key genes. Also, the results of photosynthetic efficiency and relative electric transport rate confirmed that the photosynthetic system in algal cells was severely damaged within 24 h. The algicidal effect of Raoultella sp. S1 against Microcystis aeruginosa was evaluated by analyzing the physiological response and photosynthetic system impairment of the algal cells. The concentration of microcystin-LR (MC-LR) slightly increased during the process of algal cells ruptured, and then decreased below its initial level due to the biodegradation of Raoultella sp. S1. To further investigate the algicidal mechanism of Raoultella sp. S1, the main components in the cell-free supernatant was analyzed by UHPLC-TOF-MS. Several low-molecular-weight organic acids might be responsible for the algicidal activity of Raoultella sp. S1. It is concluded that Raoultella sp. S1 has the potential to control Microcystis aeruginosa blooms.

Freshwater cyanobacteria produce highly toxic secondary metabolites, which can be transported downstream by rivers and waterways into the sea. Estuarine and coastal aquaculture sites exposed to toxic cyanobacteria raise concerns that shellfish may accumulate and transfer cyanotoxins in the food web. This study aims to describe the competitive pattern of uptake and depuration of a wide range of microcystins (MC-LR, MC-LF, MC-LW, MC-LY, [Asp3]-MC-LR/[Dha7]-MC-LR, MC-HilR) and nodularins (NOD cyclic and linear) within the common blue mussel Mytilus edulis exposed to a combined culture of Microcystis aeruginosa and Nodularia spumigena into the coastal environment.Different distribution profiles of MCs/NODs in the experimental system were observed. The majority of MCs/NODs were present intracellularly which is representative of healthy cyanobacterial cultures, with MC-LR and NOD the most abundant analogues. Higher removal rate was observed for NOD (≈96%) compared to MCs (≈50%) from the water phase. Accumulation of toxins in M. edulis was fast, reaching up to 3.4 μg/g shellfish tissue four days after the end of the 3-days exposure period, with NOD (1.72 μg/g) and MC-LR (0.74 μg/g) as the dominant toxins, followed by MC-LF (0.35 μg/g) and MC-LW (0.31 μg/g). Following the end of the exposure period depuration was incomplete after 27 days (0.49 μg/g of MCs/NODs). MCs/NODs were also present in faecal material and extrapallial fluid after 24 h of exposure with MCs the main contributors to the total cyanotoxin load in faecal material and NOD in the extrapallial fluid. Maximum concentration of MCs/NODs accumulated in a typical portion of mussels (20 mussels, ≈4 g each) was beyond greater the acute, seasonal and lifetime tolerable daily intake. Even after 27 days of depuration, consuming mussels harvested during even short term harmful algae blooms in close proximity to shellfish beds might carry a high health risk, highlighting the need for testing.