Narrow city streets surrounded by tall buildings are favorable to inducing a general effect of a “canyon” in which pollutants strongly accumulate in a relatively small area because of weak or inexistent ventilation. In this study, levels of nitrogen-oxide (NO₂), elemental carbon (EC) and organic carbon (OC) mass concentrations in PM₁₀ particles were determined to compare between seasons and different years. Daily samples were collected at one such street canyon location in the center of Zagreb in 2011, 2012 and 2013. By applying machine learning methods we showed seasonal and yearly variations of mass concentrations for carbon species in PM₁₀ and NO₂, as well as their covariations and relationships. Furthermore, we compared the predictive capabilities of five regressors (Lasso, Random Forest, AdaBoost, Support Vector Machine and Partials Least squares) with Lasso regression being the overall best performing algorithm. By showing the feature importance for each model, we revealed true predictors per target. These measurements and application of machine learning of pollutants were done for the first time at a street canyon site in the city of Zagreb, Croatia.

Health and longevity are common human goals, and environmental factors can have significant impacts on human health. This study aims to investigate the historical changes and sources of trace elements in the sediments of a typical karstic river basin with high longevity population in Hechi City, Guangxi, China and to evaluate the ecological risks of trace elements in sediments. The results showed that over the past 100 years, the contents of trace elements in the sediments were lower in the upper reaches than in the middle and lower reaches of the river. The sediments had high trace element contents in 1950–1959 and 1989–1998, while low contents appeared after 1998. These periods correspond to China’s industrial growth in the early 1950s, the Great Leap Forward movement in the late 1950s, the reform and opening-up policy implemented in the 1980s–1990s and the environmental protection policies to strengthen pollution control that have been implemented since 2000. Limestone soil and carbonate rock are the main sources of sediment in the basin. Although the geological background values of Cd and other trace elements in the basin were relatively high, the high calcium content and alkalinity of the water and sediment in the basin reduced the bioavailability of Cd and other heavy metals. The mainstream of Panyang River had a low environmental risk, but the tributary Bama River where there is dense population poses a moderate risk.

Inland waters emit large amounts of carbon dioxide (CO₂) to the atmosphere, but emissions from urban lakes are poorly understood. This study investigated seasonal and interannual variations in the partial pressure of CO₂ (pCO₂) and CO₂ flux from Lake Wuli, a small eutrophic urban lake in the heart of the Yangtze River Delta, China, based on a long-term (2000–2015) dataset. The results showed that the annual mean pCO₂ was 1030 ± 281 μatm (mean ± standard deviation) with a mean CO₂ flux of 1.1 ± 0.6 g m⁻² d⁻¹ during 2000–2015, suggesting that compared with other lakes globally, Lake Wuli was a significant source of atmospheric CO₂. Substantial interannual variability was observed, and the annual pCO₂ exhibited a decreasing trend due to improvements in water quality driven by environmental investment. Changes in ammonia nitrogen and total phosphorus concentrations together explained 90% of the observed interannual variability in pCO₂ (R² = 0.90, p < 0.01). The lake was dominated by cyanobacterial blooms and showed nonseasonal variation in pCO₂. This finding was different from those of other eutrophic lakes with seasonal variation in pCO₂, mostly because the uptake of CO₂ by algal-derived primary production was counterbalanced by the production of CO₂ by algal-derived organic carbon decomposition. Our results suggested that anthropogenic activities strongly affect lake CO₂ dynamics and that environmental investments, such as ecological restoration and reducing nutrient discharge, can significantly reduce CO₂ emissions from inland lakes. This study provides valuable information on the reduction in carbon emissions from artificially controlled eutrophic lakes and an assessment of the impact of inland water on the global carbon cycle.

Changes in soil nutrients variability could significantly interact with other global change processes (such as community dynamics, biological invasion). Global exchange and accumulation of alien species caused environmental and economic threats in the introduced ranges. Their invasion success or not in local plant communities is largely depended on the interactions and competitive outcomes with other species and environmental conditions. Here, we tested whether the interactions of nutrient variability and competitor identity influence plant performance, potential invasion success of invasive species and their co-existence with native species. In both greenhouse and field experiment, we subjected three congeneric and naturally co-occurring pairs of invasive alien and native clonal plants in China to different nutrient variability (constant high, multiple pulses and/or single pulse) and competitor identity (intra-specific competitors, native competitors, invasive competitors and both native & invasive competitors). Our results showed that total biomass or the increase of cover of invasive species was significantly larger than those of the native species regardless of competitor identity. Native competitors significantly decreased biomass proportion of native species, but did not affect that of invasive species. The whole community with invasive target species accumulated more total biomass than with native species under multiple pulses nutrient when with the native competitors. Invasive species produced significantly higher biomass proportion than natives under all competitor identity treatments except for native & invasive competitors. Multiple mixed competitors (i.e. native & invasive competitors) decreased the plant performance and dominance of invasive target species, to some extent, thus construction of multi-species competition might facilitate coexistence of native and invasive species in communities. Interactions between native competitors or native & invasive competitors, and nutrient variability play important roles in plant performance and potential invasion success in communities. Multiple invasional interference may have significant implications for the co-existence of invasive and native species, and for management of invasive species.

To date limited information’s are available concerning unintentional productions, screening, profiling, and health risks of polychlorinated naphthalenes (PCNs) in ambient environment and occupational environment. Literature reveals that dust is a neglected environmental matrix never measured for PCNs. To our knowledge, this is the first study to investigate the concentrations and health risks of PCNs in indoor dust, air, and blood of major e-waste recycling hubs in Pakistan. Indoor air (n = 125), dust (n = 250), and serum (n = 250) samples were collected from five major e-waste hubs and their vicinity to measure 39 PCN congeners using GC-ECNI-MS. ∑₃₉PCN concentrations in indoor air, dust, and serum (worker > resident > children) samples ranged from 7.0 to 9583 pg/m³, from 0.25 to 697 ng/g, and from 0.15 to 401 pg/g lipid weight, respectively. Predominant PCN congeners in indoor air and dust were tri- and tetra-CNs, while tetra- and penta-CNs were dominant in human serum samples. The higher PCNs contribution was recorded at the recycling units, while the lower was observed at the shops of the major e-waste hubs. Higher contribution of combustion origin CNs in air, dust and human samples showed combustion sources at the major e-waste hubs, while Halowax and Aroclor based technical mixture showed minor contribution in these samples. Mean toxic equivalent (TEQ) concentrations of PCNs were 2.79E⁺⁰⁰ pg-TEQ/m³, 1.60E⁻⁰² ng-TEQ/g, 8.11E⁻⁰¹ pg-TEQ/g, 7.14E⁻⁰¹ pg-TEQ/g, and 6.37E⁻⁰¹ pg-TEQ/g for indoor air, dust, and serum samples from workers, residents, and children, respectively. In our study, CNs- 66/67 and −73 in indoor air, dust, and human serum were the great contributors to total TEQ concentrations of PCNs. This first base line data directs government and agencies to implement rules, regulation to avoid negative health outcomes and suggests further awareness in regard of provision of proper knowledge to the target population.

The distribution of polycyclic aromatic hydrocarbons (PAHs) in the surface water and sediments in five regions of the Indian Sundarbans was assessed. The capability of microbial biofilm communities to sequester PAHs in a biofilm-promoting vessel was evaluated. The total PAH concentration of water and sediments ranged from undetectable to 125 ng ml⁻¹ and 4880 to 2 × 10⁴ ng g⁻¹ dry weight respectively. The total PAHs concentration of sediments exceeded the Effects Range–Low value and the recommended Effects Range-Median values, implying the PAHs might adversely affect the biota of the Sundarbans. Pyrogenic and petrogenic sources of PAH contamination were identified in most of the sampling sites. Indigenous biofilms were cultivated in a patented biofilm-promoting culture vessel containing liquid media spiked with 16 priority PAHs. Biofilm-mediated 97–100% removal efficiency of 16 PAHs was attained in all media. There was no significant difference between the mean residual PAH from the liquid media collected from hydrophobic and hydrophilic flasks. Residual amounts of acenaphthene (Ace), anthracene (Ant), benzo(b)fluoranthene [B(b)F], benzo(a)pyrene [B(a)P] and benzo(g,h,i)perylene [B(g,h,i)P] showed differences when cultivated in hydrophobic and hydrophilic flasks. The mean residual amounts of total PAHs extracted from biofilm biomasses were variable. A biofilm obtained from a specific sampling site cultured in the hydrophobic flask showed higher PAH sequestration when compared to the removal attained in the hydrophilic flask. Relative abundances of different microbial communities in PAH-sequestering biofilms revealed bacterial phyla including Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria, Chloroflexi and Planctomycetes as well as members of Ascomycota phylum of fungi. The dominance of Candida tropicalis, Clostridium butyricum, Sphingobacterium multivorum and Paecilomyces fulvus were established.

We used the Weather Research and Forecasting Model coupled with Chemistry (WRF-Chem) to simulate elemental carbon (EC) concentrations in Thailand in 2017. The goals were to quantify the respective contributions of local emissions and regional transport outside Thailand to EC pollution in Thailand, and to identify the most effective emission control strategy for decreasing EC pollution. The simulated EC concentrations in Chiang Mai, Bangkok, and Phuket were comparable with the observation data. The correlation coefficient between the simulated and observed EC concentrations was 0.84, providing a good basis for evaluating EC sources in Thailand. The simulated mean EC concentration over the whole country was the highest (1.38 μg m⁻³) in spring, and the lowest (0.51 μg m⁻³) in summer. We conducted several sensitivity simulations to evaluate EC sources. Local emissions (including anthropogenic and biomass burning emissions) and regional transport outside Thailand contributed 81.2% and 18.8% to the annual mean EC concentrations, respectively, indicating that local sources played the dominant role for EC pollution in Thailand. Among the local sources, anthropogenic emissions (including the industry, power plant, residential, and transportation sectors) and biomass burning contributed 75.1% and 6.1% to the annual mean EC concentrations, respectively. As the anthropogenic emissions dominated the EC pollution, we performed four sensitivity simulations by reducing 30% of the emissions from each of the industry, power plant, residential, and transportation sectors in Thailand. The results indicated that controlling transportation emissions in Thailand was the most effective way in reducing the EC pollution. The 30% reduction of transportation emissions decreased the annual mean EC concentrations by 12.1%. In contrast, 30% reductions of the residential, industry, and power plant emissions caused 8.4%, 6.4%, and 4.0% decreases in the annual mean EC concentrations, respectively. The model results could potentially provide useful information for air pollution control strategies in Thailand.

Glandless cotton can be grown to obtain cotton seeds free of toxic gossypol for use as both food and feed. However, they are not grown normally due to their lesser productivity and higher susceptibility to biotic stress. Great attention has been paid to biotic stresses rather than abiotic stresses on glandless cotton. Chromium (Cr) is a common pollutant of soil and considered a serious threat to plants due to its adverse effects on different functions. Although numerous studies are available on the toxicity of Cr⁶⁺ in various plants. However, its adverse effects and mechanism of toxicity in glandless cotton can seldom be found in the literature. This study examined the Cr⁶⁺ effect on glandless cotton in comparison to glanded cotton. Four pairs of glanded and glandless cotton near-isogenic lines (NILs) were exposed to different doses (0, 10, 50, and 100 μM/L) of Cr⁶⁺ for seven days, and biochemical, physiological, molecular, and ultrastructure changes were observed, which were significantly affected by Cr⁶⁺ at high concentrations in all NILs. The effect of Cr⁶⁺ on ionic contents shows the same trend in glanded and glandless NILs except for manganese (Mn²⁺) that show inhibition in glandless (ZMS-12w and Coker-312w) and enhance in the glanded NIL (ZMS-17). The gene expression of superoxide dismutase (SOD) and peroxidase (POD) revealed similar trends as enzyme activities in glandless NILs. The principal component analysis (PCA) and Agglomerative hierarchical clustering (AHC) results of all NILs from morpho-physiological traits, cluster ZMS-16, and ZMS-17 into Cr⁶⁺ sensitive group. While the glandless NILs have the potential to cope with the Cr toxicity by increasing the antioxidant enzyme activity and their gene expression. This study also revealed that Cr⁶⁺ tolerance in cotton is genotypic and has an independent mechanism in the root that not related to low gossypol.

Butylated hydroxytoluene (BHT) is a synthetic phenolic antioxidant that has been used as an additive for fat- or oil-containing foods. The exposure index value increases with extended usage of the chemical. Further, estimated total amount of BHT could exceed standard regulation, considering dietary intake or another exposure. Although BHT may induce side effects in reproductive systems, adequate research had not yet been performed to confirm them. In this study, we investigated the effects of BHT on mouse Leydig cells (TM3), which are components of testis. Our results indicated that BHT suppressed cellular proliferation and induced cell cycle arrest in TM3 cells. Moreover, BHT hampered cytosolic and mitochondrial calcium homeostasis in TM3 cells. Furthermore, BHT treatment led to endoplasmic reticulum (ER) stress and DNA fragmentation, simultaneously stimulating intrinsic apoptosis signal transduction. To elucidate the mode of action of BHT on Leydig cells, we performed western blot analysis and confirmed the activation of the PI3K/AKT and MAPK pathways. Collectively, our results demonstrated that BHT has toxic effects on mouse Leydig cells via induction of calcium dysregulation and ER-mitochondria dysfunction.

The increasing production and applications of graphene oxide (GO, a novel carbon nanomaterial) have raised numerous environmental concerns regarding its ecological risks. Triphenyl phosphate (TPhP) disperses in water and poses an increasing hazard to the ecosystem and human health. It is critical to study the environmental responses and molecular mechanisms of GO and TPhP together to assess both chemicals; however, this information is lacking. The present work revealed that GO promoted the bioaccumulation of TPhP in zebrafish larvae by 5.0%–24.3%. The TPhP-induced growth inhibition of embryos (malformation, mortality, heartbeat, and spontaneous movement) at environmentally relevant concentrations was significantly amplified by GO, and these results were supported by the downregulated levels of genes and proteins associated with cytoskeletal construction and cartilage and eye development. TPhP induced negligible alterations in the genes or proteins involved in oxidative stress and apoptosis, but those related proteins were all upregulated by GO. GO and TPhP coexposure activated the mTOR signaling pathway and subsequently promoted apoptosis in zebrafish by potentiating the oxidative stress induced by TPhP, presenting synergistic toxicity. These findings highlight the potential risks and specific molecular mechanisms of combining emerging carbon nanomaterials with coexisting organic contaminants.