Neonicotinoids pollution poses a serious threat to aquatic ecosystems. However, there is currently little knowledge about how neonicotinoids are transferred from the agricultural environment to the aquatic environment. Here, we conducted in situ high-frequency monitoring of neonicotinoids in soil-water systems along the hydrological flow path during rainfall to explore the horizontal and vertical transport mechanisms of neonicotinoids. The collected samples included 240 surface runoff, 128 subsurface runoff, 60 eroded sediment, 120 soil and 144 soil solution, which were used to analyse neonicotinoids concentrations. Surface runoff, subsurface runoff and eroded sediment were the three main paths for the horizontal migration of neonicotinoids. In the CK (citrus orchards without grass cover) and grass-covered citrus orchards, there are 15.89% and 2.29% of the applied neonicotinoids were transported with surface runoff, respectively. While in the CK and grass-covered citrus orchards, there are only 1.23% and 0.19% of the applied neonicotinoids were transported with eroded sediment and subsurface runoff. Although the amount of neonicotinoids lost along with eroded sediment was small, the concentration of neonicotinoids in eroded sediment was two orders of magnitude higher than the concentration of neonicotinoids in sediments of the surface water. Meanwhile, neonicotinoids migrated vertically in soil due to water infiltration. In the CK and grass-covered citrus orchards, there are 57.64% and 24.36% of the applied neonicotinoids were retained in soil and soil solution, respectively, and their concentration decreased as soil depth increased. Another noteworthy phenomenon is that more neonicotinoids migrated to deeper soil layers under grass cover compared with no grass cover because grass roots promoted the formation of cracks and vertical preferential flow. Our results are expected to improve the accuracy of neonicotinoids pollution prediction by considering migration paths, including surface and subsurface runoff and eroded sediment.

The San Joaquin Valley (SJV) of California has suffered persistent particulate matter (PM) pollution despite many years of control efforts. To further understand the chemical drivers of this problem and to support the development of State Implementation Plan for PM, a time-of-flight aerosol chemical speciation monitor (ToF-ACSM) outfitted with a PM₂.₅ lens and a capture vaporizer has been deployed at the Fresno-Garland air monitoring site of the California Air Resource Board (CARB) since Oct. 2018. The instrument measured non-refractory species in PM₂.₅ continuously at 10-min resolution. In this study, the data acquired from Oct. 2018 to May 2019 were analyzed to investigate the chemical characteristics, sources and atmospheric processes of PM₂.₅ in the SJV. Comparisons of the ToF-ACSM measurement with various co-located aerosol instruments show good agreements. The inter-comparisons indicated that PM₂.₅ in Fresno was dominated by submicron particles during the winter whereas refractory species accounted for a major fraction of PM₂.₅ mass during the autumn associated with elevated PM₁₀ loadings. A rolling window positive matrix factorization analysis was applied to the organic aerosol (OA) mass spectra using the Multilinear Engine (ME-2) algorithm. Three distinct OA sources were identified, including vehicle emissions, local and regional biomass burning, and formation of oxygenated species. There were significant seasonal variations in PM₂.₅ composition and sources. During the winter, residential wood burning and oxidation of nitrogen oxides were major contributors to the occurrence of haze episodes with PM₂.₅ dominated by biomass burning OA and nitrate. In autumn, agricultural activities and wildfires were found to be the main cause of PM pollution. PM₂.₅ concentrations decreased significantly after spring and were dominated by oxygenated OA during March to May. Our results highlight the importance of using seasonally dependent control strategies to mitigate PM pollution in the SJV.

This study examined the effect of tetracycline addition on arsenic (As) mobilization and biotransformation in two contrasting soils (upland soil and paddy soil) under flooded conditions. The soils with added tetracycline (0–50 mg kg⁻¹) were incubated for 30 days, and soil properties and microbial functional genes over time were quantified. Tetracycline significantly promoted As reduction and As release into porewater in both soils. The enhancement had resulted from an increase in the concentration of dissolved organic carbon and a decrease in soil redox potential. Tetracycline also increased the abundances of As-reducing genes (arsC and arrA) and the relative abundances of As-reducing bacteria Streptomyces, Bacillus, Burkholderia, Clostridium and Rhodococcus, all of which have been found resistant to tetracycline. These genera play a key part in stimulating As reduction in the presence of tetracycline. The study indicated the significance of tetracycline in the biochemical behavior of As in flooded soils and provided new insights into the potential effects of tetracycline on the quality and safety of agricultural products in the future.

Toxicological and epidemiological studies implicate exposure to dithiocarbamate (DTC) fungicides in adverse health outcomes. However, there is limited information about human exposure to these chemicals. This systematic review determined to which extent human populations worldwide, including children, pregnant women, and adults, are exposed environmentally or occupationally to DTC pesticides and how these exposures compare to the NHANES 2003–2008 population, using urinary ETU data as an outcome measure. PubMed, Embase, and SciFinder were searched using the keywords “ethylenethiourea” or CAS No.: 96-45-7, and urine or urinary. Duplicates and irrelevant studies were removed from the search results based on predetermined exclusion criteria. This screening process identified 17 relevant papers. One additional paper was found independent of this search. Data from studies were extracted using a pre-established data collection form. Ten, two, and five manuscripts reported urinary levels in environmentally exposed adults, children, and pregnant women, respectively. Median ETU levels ranged from 0.15 to 4.7 μg/g creatinine in adults (1994–2017), 0.24–0.83 μg/g creatinine in children (2011), and 2.6–5.24 ng/ml in pregnant women (2011). Eight studies reported urinary ETU levels in mostly agriculturally exposed populations, with median ETU levels ranging from 0.42 to 49.6 μg/g creatinine (1999–2011). With one exception, all studies were conducted between 1994 and 2011. ETU levels in the NHANES 2003–2008 population appeared to be generally lower than most studies identified in this review. This finding suggests that, historically, DTC fungicide exposures in the general population of high-income countries, such as the US, were low, whereas agricultural populations may have experienced higher exposure. Unfortunately, more recent exposure data are missing, especially in countries where DTC pesticides are not being phased out.

The iron and steel industry (ISI) is one of the most energy-intensive industries in China, which makes a substantial contribution to the emissions of air pollutants. Among the various manufacturing processes, sintering is the major emitting process, which shares over half of the emissions of sulfur dioxide (SO₂), nitrogen oxide (NOₓ) and particulate matter (PM) for the entire industry. In this study we made a comprehensive evaluation of the air pollutant emissions from the sintering process of China's ISI in 2017 based on the Continuous Emission Monitoring System (CEMS) database and estimated the future reduction potentials. We found that there was a general decreasing trend of emission concentrations in the sintering flue gas in response to the strengthened emission control policies, but the mild increase of the oxygen content in the second half of the year flattened the decreasing trend, indicating the necessity for simultaneous control of the oxygen content in the flue gas. Despite the relative high standard-reaching rates of 90% to the emission concentration limits in GB 28662-2012, the standard-reaching rates to the ultra-low emission standards were only 12%, 40% and 27% for NOₓ, SO₂ and PM respectively, with the lowest value mostly occurred in the western provinces. In 2017, the NOₓ, SO₂ and PM emissions from the sintering process were 378.6 kt, 169.0 kt and 51.9 kt, respectively. If the ultra-low emission standards were met, the corresponding NOₓ, SO₂ and PM emissions would decrease by 69.9%, 52.9%, and 56.4% respectively, illustrating large emission reducing potentials by achieving the ultra-low emission standards.

A pyrene-degrading consortium OPK containing Mycolicibacterium strains PO1 and PO2, Novosphingobium pentaromativorans PY1 and Bacillus subtilis FW1 effectively biodegraded medium- and long-chain alkanes as well as mixed hydrocarbons in crude oil. The detection of alkB and CYP153 genes in the genome of OPK members supports its phenotypic ability to effectively degrade a broad range of saturated hydrocarbons in crude oil. Zeolite-immobilized OPK was developed as a ready-to-use bioproduct and it exhibited 74% removal of 1000 mg L⁻¹ crude oil within 96 h in sterilized seawater without nutrient supplementation and maintained high crude oil-removal activity under a broad range of pH values (5.0–9.0), temperatures (30–40 °C) and salinities (20–60‰). In addition, the immobilized OPK retained a high crude oil removal efficacy in semicontinuous experiments and showed reusability for at least 5 cycles. Remarkably, bioaugmentation with zeolite-immobilized OPK in sandy soil microcosms significantly increased crude oil (10,000 mg kg⁻¹ soil) removal from 45% to 80.67% within 21 days compared to biostimulation and natural attenuation. Moreover, bioaugmentation with exogenous immobilized OPK stimulated an increase in the relative abundances of Alcanivorax genus, indigenous hydrocarbon-degrading bacteria, which in turn enhanced removal efficiency of crude oil contamination from sandy soil microcosms. The results indicate positive interactions between the bioaugmented immobilized consortium, harboring Mycolicibacterium as a key player, and indigenous Alcanivorax, which exhibited crucial functions for improving crude oil removal efficacy. The knowledge obtained forms an important basis for further synthesis and handling of a promising bio-based product for enhancing the in situ bioremediation of crude oil-polluted marine environments.

Although biomass fuel has always been regarded as a source of sustainable energy, it potentially emits polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs). This study investigated PCDD/F emissions from industrial boilers fired with three types of biomass fuel (i.e., bagasse, coffee residue, and biomass pellets) via stack sampling and laboratory analysis. The measured mass concentrations of PCDD/Fs varied among the boilers from 0.0491 to 12.7 ng Nm⁻³ (11% O₂), with the calculated average international toxic equivalent quantity (I-TEQ) from 0.00195 to 1.71 ng I-TEQ Nm⁻³ (11% O₂). Some of them were beyond the limit value for municipal waste incineration. 2,3,4,7,8-PeCDF could be used as a good indicator of dioxin-induced toxicity of stack flue gases from biomass-fired boilers. The PCDFs/PCDDs ratios were more than 1, likely indicating the formation of dioxins in the boilers favored by de novo synthesis. The emission factor (EF) of total PCDD/Fs averaged 5.35 ng I-TEQ kg⁻¹ air-dry biomass (equivalent to 39.0 ng kg⁻¹ air-dry biomass). Specifically, the mean EF was 6.94 ng I-TEQ kg⁻¹ (52.6 ng kg⁻¹) for biomass-pellet-fired boiler, 11.8 ng I-TEQ kg⁻¹ (74.6 ng kg⁻¹) for coffee-residue -fired boiler, and 0.0277 ng I-TEQ kg⁻¹ (0.489 ng kg⁻¹) for bagasse-fired boilers. The annual PCDD/F emission was estimated to be 208 g I-TEQ in 2020 in China, accounting for approximately 2% of the total national annual emission of PCDD/Fs. The results can be used to develop PCDD/Fs emission inventories and offer valuable insights to authorities regarding utilizing biomass in industry in the future.

Evidence suggests that the invasion of Spartina alterniflora (S. alterniflora) poses potentially serious risks to the stability of coastal wetlands, an ecosystem that is extremely vulnerable to both biological and non-biological threats. However, the effects and mechanisms of sulfur (S) in mediating the growth and expansion of S. alterniflora are poorly understood, particularly when sediments are contaminated with cadmium (Cd). A 6-month greenhouse study was conducted to evaluate the mediating effect of S on Cd tolerance and growth of S. alterniflora. Treatments consisted of a factorial combination of three S rates (applied as Na₂SO₄; 0, 500, 1000 mg kg⁻¹ dry weight (DW), as S₀, S₅₀₀, and S₁₀₀₀) and four Cd rates (applied as CdCl₂; 0, 1, 2, 4 mg kg⁻¹ DW, as Cd₀, Cd₁, Cd₂, and Cd₄). Results showed that although the exogenous S supply obviously increased Cd accumulation in roots (up to 71.22 ± 6.43 mg kg⁻¹ DW) due to the decrease of Fe concentration in iron plaque (down to 4.02 ± 1.18 mg g⁻¹ DW), biomass reduction and oxidative stress in plant tissues were significantly alleviated. The addition of S significantly up-regulated the concentration of compounds related to Cd tolerance, including proline and glutathione. Therefore, the translocation of Cd was restricted, and plant growth was not impacted. The present study demonstrated that the exogenous sulfur supply could promote the growth of S. alterniflora and enhance its tolerance to Cd. Therefore, under the effects of S. alterniflora, the increased fluctuations of S pool caused by the release and deposition of S might further exacerbate S. alterniflora expansion in Cd contaminated coastal wetlands.

Although concerns have been raised about co-selection for antibiotic resistance and various antibiotics and non-antibiotic agents, the data on their association in urban sludge is still limited. In addition, antibiotic contamination can result in not only the toxicity but also the antibiotic resistance. In this study, the first large-scale identification of antibiotics and non-antibiotic agents concern for co-selection of resistance against antibiotics was conducted in urban sludge. Co-occurrence analysis showed that antibiotic resistance genes (ARGs) had no significant correlation with the corresponding antibiotics. Therefore, the results of co-occurrence analysis based on antibiotic concentration and ARG abundance were always ambiguous and difficult to interpret. However, antibiotic resistance was positively correlated with highly toxic compounds such as diclofenac, enrofloxacin and nicotine, suggesting that environmental contaminants might influence antibiotic resistance while exerting toxicity through mechanisms such as changes in microbial community and enzyme activity. The close correlation between class 1 integrase gene (intI1) and diclofenac/enrofloxacin indicated that the co-selection scenario between environmental contaminants and ARGs was likely mediated via intI1. In total, the derived co-occurrence patterns improve our understanding of the co-selection between ARGs, antibiotics and non-antibiotic agents, and also reaffirm the importance of potential role of non-antibiotic agents in the global spread of antibiotic resistance.

With the wide utilization of organophosphate esters (OPEs) in recent years, OPEs have been detected more frequently in the aquatic environment. However, the distribution of OPEs in drinking source water has rarely been investigated across a large region. In this study, the occurrence and distribution of 13 OPEs were investigated in 23 source water sites from Northeast to Southeast (spacing greater than 3320 km) with a direct injection ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS) method. Total OPEs ranged from 218.8 to 636.6 ng/L, with a mean of 380.8 ng/L. The average detected concentration of OPEs in southern cities was higher than that in northern cities. Chlorinated OPEs accounted for 64.74% of the total concentration. Triethyl phosphate (TEP), tri (2-chloroethyl) phosphate (TCEP), and tri (chloropropyl) phosphate (TCPP) were detected in all water samples. Rainfall is a significant factor that affects the OPE concentration (less rainfall, higher concentration). China's OPE concentrations have rapidly reached a median level when compared to those of other countries. Ecological risk assessment showed that most OPEs have no or low risk to organisms (fish, crustacea, algae), except tricresyl phosphate (TCP), which is medium risk. The risk of OPEs in less-rain regions needs to be of greater concern, especially TCP.