On 15th April 2019, Parisians watched in shock as Notre-Dame de Paris, the iconic cathedral that has towered over their city for almost 900 years, was engulfed in flames. Although flames destroyed the spire and considerably weakened the structure, no human lives were lost. However, as some amounts of lead volatilized and deposited in the surrounding areas, fears of potential intoxication began to rise. We investigated the impact of this fire on the blood lead levels of adults in Paris according to the distance between the cathedral and where they live or work. The geometric mean of blood lead levels of the study population was 1.49μg/dl (95% CI [1.38–1.62]) with a prevalence of blood lead levels≥5.0μg/dL of 5.0%. Despite the early legitimate fears of intoxication, the fire that destroyed a significant part of the Notre-Dame cathedral did not increase the blood lead levels of adults living and working in the vicinity.

As a result of historical industrial activity, the sediments in the inner harbor of Owen Sound Bay in the northeastern part of Lake Huron in Ontario, Canada are contaminated with organic compounds. The present study showed that the concentrations of Ʃ PAH₁₆₋EPA in all sediments in the inner harbor were above the sediment quality guidelines for the province of Ontario, Canada, with mean Ʃ PAH₁₆₋EPA concentrations at the most contaminated site of 46,000 μg/kg dry weight. The concentrations of polychlorinated biphenyls, brominated diphenyl ethers, and organochlorine compounds were all below sediment quality guidelines. The patterns of PAH and alkyl-PAH compounds in sediment cores indicated that contamination is from mixed sources, with a strong indication of pyrogenic contamination from industries that used to operate in the area, including a coal gasification plant. Other areas of the bay are impacted by petrogenic contamination, potentially from spills of fuel. The even distribution of PAH and alkyl-PAH compounds throughout core profiles at depths up to 25 cm indicates that this is a dynamic system and contaminated sediments are not being covered by deposition of less contaminated sediments. This study illustrates the value of determining the patterns of both PAH and alkyl-PAH compounds in sediments for regulatory purposes and also for forensic source tracking.

Biofuel production via pyrolysis has received increasing interest as a promising solution for utilization of now wasted food residue. In this study, the fast pyrolysis of mixed food waste (MFW) was performed in a bubbling fluidized-bed reactor. This was done under different operating conditions (reaction temperatures and carrier gas flow rate) that influence product distribution and bio-oil composition. The highest liquid yield (49.05 wt%) was observed at a pyrolysis temperature of 475 °C. It was also found that the quality of pyrolysis bio-oils (POs) could be improved using catalysts. The catalytic fast pyrolysis of MFW was studied to upgrade the pyrolysis vapor, using dolomite, red mud, and HZSM-5. The higher heating values (HHVs) of the catalytic pyrolysis bio-oils (CPOs) ranged between 30.47 and 35.69 MJ/kg, which are higher than the HHVs of non-catalytic pyrolysis bio-oils (27.69–31.58 MJ/kg). The major components of the bio-oils were fatty acids, N-containing compounds, and derivatives of phenol. The selectivity for bio-oil components varied depending on the catalysts. In the presence of the catalysts, the oxygen was removed from oxygenates via moisture, CO₂, and CO. The CPOs contained aliphatic hydrocarbons, polycyclic aromatic compounds (such as naphthalene), pyridine derivatives, and light oxygenates (cyclic alkenes and ketones).

The present study showed that syntrophic associations in a defined bacterial consortium, named OPK, containing Mycolicibacterium strains PO1 and PO2, Novosphingobium pentaromativorans PY1 and Bacillus subtilis FW1, led to effective pyrene degradation over a wide range of pH values, temperatures and salinities, as well as in the presence of a second polycyclic aromatic hydrocarbon (PAH). Anthracene, phenanthrene or fluorene facilitated complete pyrene degradation within 9 days, while fluoranthene delayed pyrene degradation. Interestingly, fluoranthene degradation was enhanced in the presence of pyrene. Transcriptome analysis confirmed that Mycolicibacterium strains were the key PAH-degraders during the cometabolism of pyrene and fluoranthene. Notably, the transcription of genes encoding pyrene-degrading enzymes were shown to be important for enhanced fluoranthene degradation. NidAB was the major initial oxygenase involved in the degradation of pyrene and fluoranthene mixture. Other functional genes encoding ribosomal proteins, an iron transporter, ABC transporters and stress response proteins were induced in strains PO1 and PO2. Furthermore, an intermediate pyrene-degrading Novosphingobium strain contributed to protocatechuate degradation. The results demonstrated that synergistic interactions among the bacterial members (PO1, PO2 and PY1) of the consortium OPK promoted the simultaneous degradation of two high molecular weight (HMW) PAHs.

Ambient air pollution is one of the leading environmental risk factors to human health, largely offsetting economic growth. This study evaluated health burden and cost associated with the short-term and long-term exposure of major air pollutants (fine particulate matter [PM₂.₅] and ozone [O₃]) during 2013–2018. We developed a database of gridded daily and annual PM₂.₅ and O₃ exposure in China at 15 km × 15 km resolution. Then, we estimated the age- and cause-specific premature deaths and quantified related health damage with an age-adjusted value of statistical life (VSL) measure. The health cost estimated in this study captured direct cost, indirect cost and intangible cost of the premature death attributable to ambient PM₂.₅ and O₃. We found that the national premature deaths attributable to long-term and short-term exposure to PM₂.₅ decreased by 15% and 59%, whereas the national premature deaths attributable to long-term and short-term exposure to O₃ increased by 36% and 94%. Despite a 15% reduction of attributable deaths, the health cost attributable to long-term exposure to PM₂.₅ did not change significantly as a result of GDP growth and population aging. On the other hand, the long-term O₃ related health cost in 2018 doubled that in 2013. Our study suggests that while premature deaths fell as a result of China’s clean air actions, the health costs of air pollution remained high. The growing trends of O₃ highlighted the needs for strategies to reduce both PM₂.₅ and O₃ emissions, for the sake of public health and social well-being in China.

Hydrogen sulfide (H₂S) is well known as a gaseous signal in response to heavy metal stress, while methane (CH₄), the most prevalent greenhouse gas, confers cadmium (Cd) tolerance. In this report, the causal link between CH₄ and H₂S controlling Cd tolerance in alfalfa (Medicago sativa) plants was assessed. Our results observed that the administration of CH₄ not only intensifies H₂S metabolism, but also attenuates Cd-triggered growth inhibition in alfalfa seedlings, which were parallel to the alleviated roles in the redox imbalance and cell death in root tissues. Above results were not observed in roots after the removal of endogenous H₂S, either in the presence of either hypotaurine (HT; a H₂S scavenger) or DL-propargylglycine (PAG; a H₂S biosynthesis inhibitor). Using in situ noninvasive microtest technology (NMT) and inductively coupled plasma mass spectroscopy (ICP-MS), subsequent results confirmed the participation of H₂S in CH₄-inhibited Cd influx and accumulation in roots, which could be explained by reestablishing glutathione (GSH) pool (reduced/oxidized GSH and homoglutathione) homeostasis and promoting antioxidant defence. Overall, our results clearly revealed that H₂S operates downstream of CH₄ enhancing tolerance against Cd stress, which are significant for both fundamental and applied plant biology.

Many prey organisms adaptively respond to predation risk by inducible defenses with underlying tradeoffs in resource allocation. Cyanobacterial blooms expose zooplankton to poor food conditions, affecting the herbivores’ fitness. Given the interferences on resources allocation and life history traits, poor-quality cyanobacteria are predicted to affect the adaptive predator-induced responses in zooplankton. Here, we exposed two clones (i.e., clones SH and ZJ) of the cladoceran Daphnia mitsukuri to different combinations of fish predation cues and diets containing toxic Microcystis aeruginosa (0%–30%). D. mitsukuri matured at a small size and had elongated relative tail spine as adaptive responses to fish cues. Despite the comparable tail spine defense, fish cue-induced changes in growth and reproduction in the clone SH were more pronounced than those in the clone ZJ under no M. aeruginosa. Animals accumulated microcystin in the whole body with increasing abundance of M. aeruginosa. However, the inducible enhanced tail spine allometry was not affected, resulting in unchanged tail spine defense by Daphnia under all M. aeruginosa treatments. By contrast, M. aeruginosa remarkably decreased the adaptive maturation size and the offspring number in all animals. However, the inducible reproductive effort tended to increase or remain unchanged depending on clones associated with the constant or decreased responses of the somatic growth effort under increasing M. aeruginosa. Our results suggested that toxic M. aeruginosa did not alter the resource allocation to antipredator morphological defense but affected the somatic growth and reproduction in D. mitsukuri under fish cues. The present study highlights the different effects of toxic cyanobacteria on adaptive predator-induced responses in zooplankton, promoting the understanding for the morphological defense-mediated predator–prey interactions in eutrophic environments.

Nanoparticulate mineral UV filters, such as titanium dioxide (TiO₂) nanocomposites, are being increasingly used in sunscreens as an alternative to organic UV filters. However, there is still a lack of understanding regarding their fate and behavior in aquatic environments and potential environmental impacts after being released from a bather’s skin during recreational activities. In this work, we assessed the release, fate, and transformation of two commercial nanocomposite TiO₂ UV filters, one hydrophobic and one hydrophilic, in ultrapure water and simulated fresh- and seawater. The hydrophobic TiO₂ nanocomposite, T-SA, was coated with a primary Al₂O₃ photopassivation layer and a secondary stearic acid layer, while the hydrophilic TiO₂ nanocomposite, T-SiO₂, was coated with a single SiO₂ photopassivation layer. The influence of the sunscreen formulation was examined by dispersing the TiO₂ nanocomposites in their typical continuous phase (i.e., oil for T-SA and water for T-SiO₂) before introduction into the aqueous system. After 48 h of aqueous aging and 48 h of settling, 88–99% of the hydrophobic T-SA remained floating on top of the water column in all aqueous systems. On the other hand, 100% of the hydrophilic T-SiO₂ settled out of the water column in the fresh- and seawaters. With respect to the photopassivation coatings, no loss of the T-SA Al₂O₃ layer was detected after aqueous aging, but 99–100% dissolution of the SiO₂ layer on the T-SiO₂ nanocomposite was observed after 48 h in the fresh- and seawaters. This dissolution left behind T-SiO₂ by-products exhibiting a photocatalytic activity similar to that of bare rutile TiO₂. Overall, the results demonstrated that the TiO₂ surface coating and sunscreen formulation type drive environmental behavior and fate and that loss of the passivation layer can result in potentially harmful, photoactive by-products. These insights will help guide regulations and assist manufacturers in developing more environmentally safe sunscreens.

One of the causes of the global decline of amphibians is agricultural activity, responsible for causing habitat fragmentation and bringing a range of agrochemicals and fertilizers in the environment, compounds with a potential disrupting effect on non-target organisms, such as frogs. Exposure to these compounds has numerous harmful effects on the testes of these animals, which can compromise reproduction and, consequently, the maintenance of their communities. In this context, we compared the morphology and morphometry of the testes of three species of neotropical anurans (Physalaemus cuvieri, Dendropsophus minutus, and Boana albopunctata) from an agricultural area and a conservation unit. Histologically, the testicular morphology of the species was similar for both environments; however, morphometrically, there was a difference in the measured testicular parameters (locular area and area of spermatogenic cysts). Physalaemus cuvieri presented higher averages of locular and spermatogonial area in the agricultural environment, whereas the area occupied by the spermatozoa was smaller. Additionally, the testicular pigmentation, which is only present in this species, was greater in animal from the agricultural area. In D. minutus, the locular, spermatogonial, and sperm areas showed lower values in the agricultural area, whereas in B. albopunctata, the opposite pattern was found, with the area of the locule, spermatocytes, and spermatozoids being higher. Agricultural activities influence the testicular metric parameters in different species, and our results suggest that D. minutus is most sensitive to anthropic pressures. The least sensitive species is B. albopunctata. We highlight the importance of evaluating different species, since each species responds differently to agricultural activities.

The effects of fabric filters and desulfurization systems during secondary copper smelting on polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) concentrations, emission coefficients, and profiles were studied in an oxygen-rich smelting furnace and an anode furnace. In the anode furnace, the toxic equivalent (TEQ) concentration ranges were 0.106–1.04 ng World Health Organization (WHO)-TEQ/m³ at the fabric filters inlet and 0.027–0.17 ng WHO-TEQ/m³ at the outlet. For the oxygen-rich smelting furnace, the TEQ concentration ranges were 1.21–1.93 and 0.010–0.019 ng WHO-TEQ/m³ at the desulfurization system inlet and outlet, respectively. The TEQs in the outlet stack gases of the desulfurization system from the anode furnace were 0.0041–0.016 ng WHO-TEQ/m³. It is likely that PCDD/Fs that were taken away from the stack gases were adsorbed by the fly ash and gypsum. Solid residues were the dominant release routes for PCDD/Fs. PCDD/Fs congener and homologue profiles of stack gases from different smelting stages were similar. The contributions of more chlorinated homologues from the anode furnace decreased observably after the stack gases passed through the fabric filters. However, the desulfurization process did not greatly change the PCDD/Fs homologue profiles. Overall, both the fabric filters and desulfurization systems showed excellent removal efficiencies for PCDD/Fs in the stack gases, which reduced the TEQ emissions to well below the 0.5 ng WHO-TEQ/m³ to achieve standard discharge.