A novel denitrifying bacterium YSF15 was isolated from the Lijiahe Reservoir in Xi’an and identified as Comamonas sp. It exhibited excellent nitrogen removal ability under low C/N conditions (C/N = 2.5) and 94.01% of nitrate was removed in 18 h, with no accumulation of nitrite. PCR amplification and nitrogen balance experiments were carried out, showing that 68.92% of initial nitrogen was removed as gas products and the nitrogen removal path was determined to be NO3−-N→NO2−-N→NO→N2O→N2. Scanning electron microscopy and three-dimensional fluorescence spectroscopy were used to track extracellular polymeric substances (EPS). The results show that complete-denitrification under low C/N conditions is associated with EPS, which may provide a reserve carbon source in extreme environments. These findings reveal that Comamonas sp. YSF15 can provide novel basic materials and a theoretical basis for wastewater bioremediation under low C/N conditions.

Exhaust emissions from diesel vehicles are significant sources of air pollution. In this study, particle number emissions and size distributions of a modern Euro 5b -compliant diesel passenger car exhaust were measured under the NEDC and US06 standard cycles as well as during different transient driving cycles. The measurements were conducted on a chassis dynamometer; in addition, the transient cycles were repeated on-road by a chase method. Since the diesel particulate filter (DPF) removed practically all particles from the engine exhaust, it was by-passed during most of the measurements in order to determine effects of lubricant on the engine-out exhaust aerosol. Driving conditions and lubricant properties strongly affected exhaust emissions, especially the number emissions and volatility properties of particles. During acceleration and steady speeds particle emissions consisted of non-volatile soot particles mainly larger than ∼50 nm independently of the lubricant used. Instead, during engine motoring particle number size distribution was bimodal with the modes peaking at 10–20 nm and 100 nm. Thermal treatment indicated that the larger mode consisted of non-volatile particles, whereas the nanoparticles had a non-volatile core with volatile material condensed on the surfaces; approximately, 59–64% of the emitted nanoparticles evaporated. Since during engine braking the engine was not fueled, the origin of these particles is lubricant oil. The particle number emission factors over the different cycles varied from 1.0 × 10¹⁴ to 1.3 × 10¹⁵ #/km, and engine motoring related particle emissions contributed 12–65% of the total particle emissions. The results from the laboratory and on-road transient tests agreed well. According to authors’ knowledge, high particle formation during engine braking under real-world driving conditions has not been reported from diesel passenger cars.

Biofumigation is an effective, non-chemical method to control soil-borne pests and diseases and to maximize crop yield. We studied the responses of soil bacterial and fungal communities, the soil’s nutritional state and strawberry yield, when the soil was biofumigated each year for five consecutive years using fresh chicken manure (BioFum). BioFum significantly increased the soil’s NH4+-N, NO3−-N, available P and K and organic matter. Fusarium spp. and Phytophthora spp. which are known to cause plant disease, were significantly decreased after BioFum. In addition, Biofum increased the soil’s temperature, enhanced chlorophyll levels in the leaves of strawberry plants, and the soluble sugar and ascorbic acid content in strawberry fruit. We used high-throughput gene sequencing to monitor changes in the soil’s bacterial and fungal communities. Although BioFum significantly decreased the diversity of these communities, it increased the relative abundance of some biological control agents in the phylum Actinobacteria and the genera Pseudomonas, Bacillus and Chaetomium. An increase in these biological control agents would reduce the incidence of soil-borne pathogens and plant disease. Although strawberry marketable yield using BioFum was higher in the first three years, the decline in the final two years could be due to the accumulation of P and K which may have delayed flowering and fruiting. Methods to overcome yield losses using BioFum need to be developed in the future. Our research, however, showed that BioFum enhanced soil fertility, reduced the presence of soil pathogens, increased the relative abundance of beneficial bacteria and fungi and improved strawberry quality. Unlike chemical soil treatments that can cause pest and disease resistance when used continuously over many years, our multi-year research program on BioFum showed that this treatment provided significant benefits to the soil, plant and strawberry fruit.

China and the UK use different risk-based approaches to derive soil screening or guideline values (SSVs; SGVs) for contaminants. Here we compare the approaches and the derived values for 6 illustrative contaminants. China’s SSVs are derived using an approach developed in the US as follows: for carcinogens, acceptable level of risk (ACR) is set at 10−6 and the SSVs calculated as 10−6 divided by the soil exposure and toxicity data; for non-carcinogens, the hazard quotient is 1 and the SSV is calculated as 1 divided by the soil exposure and toxicity data. The UK’s SGVs are calculated by the CLEA model, for which the Average Daily Exposure (ADE) from soil sources by a specific exposure route equals the health criteria values (HCVs) for that route, whether for carcinogens or a non-carcinogens. The UK’s CLEA model is also used here to derive SSVs with Chinese input parameters. China’s SSVs, the UK’s SGVs and values for Chinese conditions derived using the UK approach were as follows (mg/kg): As, <1, 35, 20; Cd, 20, 18, 11; Cr (VI), <1, 14, 29; benzene, 1, 1, 2; toluene, 1200, 3005, 3800; ethyl-benzene, 7, 930, 1200. By comparing the differences in toxicity assessment and risk characterization, exposure assessment and parameter types in the methodologies to obtain SSVs in China and the UK, and by combining the CLEA model with Chinese parameterisation, these comparisons highlight that the difference in toxicity assessment and risk characterization methods of carcinogens results in the biggest difference in SSVs between the 2 countries. However, for non-carcinogenic substances, the difference of SSVs calculation method and SSVs is small. The difference in SSVs for carcinogenic substances is also related to the route of exposure. For volatile organic compounds, the presence of indoor respiratory exposure pathways greatly reduces the differences caused by toxicity assessment and risk characterization methods. For non-volatile substances such as heavy metals, the effects of toxicity assessment and risk characterization methods are significant. The SSV of As obtained by the CLEA model with Chinese parameters is closer to the background value of soil in China. In the management of non-volatile contaminated sites such as heavy metals in China, the CLEA model can be used for risk assessment and calculation of site specific SSVs. In the future, China can use the UK method to strengthen its toxicity assessment and risk characterization methods for carcinogenic substances, to reduce the uncertainty in the risk assessment of contaminated sites and improve the scientific management of contaminated sites.

Dibutyl phthalate (DBP), an important plastic contaminant in the environment, is known to cause organ toxicity. Although current research has shown that DBP-induced organ toxicity is associated with oxidative stress, the toxic effect of DBP on the lungs have not been fully elucidated. Therefore, we investigated the potential mechanism by which DBP induces pulmonary toxicity using a model of DBP-induced allergic airway inflammation in rats. The results showed that chronic exposure to DBP induced histopathological damage, inflammation, oxidative stress, apoptosis, and increased the protein levels of thymic stromal lymphopoietin (TSLP) and its downstream protein Janus kinase 1 (JAK1) and signal transducer and activator of transcription 6 (STAT6). Moreover, DBP exposure inhibited nuclear factor-erythroid-2-related factor 2 (Nrf2) and levels of its target genes NAD(P)H quinone oxidoreductase 1 (NQO1) and heme oxygenase-1 (HO-1). Additionally, using in vitro experiments, we found that DBP induced oxidative stress, reduced cell viability, and inhibited the Nrf2/HO-1/NQO1 pathway in mouse alveolar type II epithelial cell line. Overall, these data demonstrate that DBP induces allergic airway inflammation in rats via inhibition of the Nrf2/TSLP/JAK1 pathway.

The impact of agricultural pesticides on sensitive aquatic ecosystems is a matter of global concern. Although South Africa is the largest user of pesticides in sub-Saharan Africa, few studies have examined the toxicological threats posed by agricultural runoff, particularly to conservation areas of international importance. This study investigated the occurrence of 11 priority listed herbicides in sediments from Lake St Lucia, located on the east coast of South Africa. While characterised by exceptionally high levels of biodiversity, Lake St Lucia is affected by agricultural runoff primarily via inflow from two major rivers; the Mkhuze and Mfolozi. Sediment samples collected from Lake St Lucia and its two major fluvial inputs reveal widespread herbicide contamination of the aquatic environment. Residues were detected in the vast majority of samples analysed, with Mkhuze (27.3 ± 17 ng g⁻¹) and Mfolozi (25.6 ± 20 ng g⁻¹) sediments characterised by similar total herbicide levels, while lower concentrations were typically detected in Lake St Lucia (12.9 ± 12 ng g⁻¹). Overall, the most prominent residues detected included acetochlor (3.77 ± 1.3 ng g⁻¹), hexazinone (2.86 ± 1.4 ng g⁻¹) and metolachlor (10.1 ± 8.7 ng g⁻¹). Ecological assessment using Risk Quotients (RQs) showed that cumulative values for triazines and anilides/aniline herbicide classes presented low to medium risk for algae and aquatic invertebrate communities. Considering the biological importance of Lake St Lucia as a nursery for aquatic organisms, it is recommended that further research on the aquatic health of the system be undertaken. Additional monitoring and investigation into mitigation strategies is suggested, particularly as agricultural activities surrounding Lake St Lucia are likely to expand in the future.

Forests in Mediterranean Europe including Portugal are highly susceptible to wildfires. Freshwaters are often exposed to post-wildfire contamination that contains several toxic substances, which may impose risk to freshwater organisms and ecosystem functions. However, knowledge on the impacts of post-wildfire runoffs from different origins on freshwater biota is scarce. In forest streams, invertebrate shredders have a major contribution to aquatic detrital-based food webs, by translocating energy and nutrients from plant-litter to higher trophic levels. We investigated the leaf consumption behaviour and the responses of oxidative and neuronal stress enzymatic biomarkers in the freshwater invertebrate shredder Allogamus ligonifer after short-term exposure (96 h) to post-wildfire runoff samples from Pinus and Eucalyptus plantation forests and stream water from a burnt catchment in Portugal. Chemical analyses indicated the presence of various metals and PAHs at considerable concentrations in all samples, although the levels were higher in the runoff samples from forests than in the stream water. The shredding activity was severely inhibited by exposure to increased concentrations of post-wildfire runoff samples from both forests. The dose-response patterns of enzymatic biomarkers suggest oxidative and neuronal stress in the shredders upon exposure to increasing concentrations of post-wildfire runoffs. The impacts were more pronounced for the runoffs from the burnt forests. Moreover, the response patterns suggest that the energy from the feeding activity of shredders might have contributed to alleviate the stress in A. ligonifer. Overall, the outcomes suggest that the post-wildfire contamination can induce sublethal effects on invertebrate shredders with impacts on key ecological processes in streams.

Globally, wildfires are increasing in frequency and severity, exposing populations to toxic trace elements stored within forests. Trace element and Pb isotope compositions in aerosols (n = 87) from four major wildfires near Sydney, Australia (1994-2004) were evaluated (Mood’s median test) to determine any significant differences in concentration before, during or after wildfires. The US EPA’s positive matrix factorization (PMF) model was used to distinguish a wildfire-related particulate source factor. Atmospheric concentrations of Cd and Mn were approximately 2.5 times higher during fire periods. PMF modelling distinguished a soil factor (Ca, Si, Ti and Zn) and an anthropogenically-sourced factor (Cd, Pb). The Cd, Pb anthropogenic factor was present at twice the concentration during wildfire periods (compared to before or after). Lead isotopic analyses of aerosols revealed that former leaded gasoline depositions were subject to remobilization during post-2000 wildfire periods. Trace element increases during wildfires are unlikely to exceed health-based criteria.

Di-(2-ethylhexyl) phthalate (DEHP) has been utilized in many products for years. DEHP exposure has been linked to cardiovascular diseases (CVD) and its risk factors. Recent evidence has found a crucial role for epigenetics, including DNA methylation, in CVD. Moreover, DEHP exposure has proved to alter DNA methylation in epidemiological studies. However, the interplay between DEHP exposure, global DNA methylation, and atherosclerosis has never been reported. In this current study, we enrolled 793 participants (12–30 years) from a Taiwanese population to investigate the association between concentrations of DEHP metabolites, 5mdC/dG (global DNA methylation marker) and the carotid intima-media thickness (CIMT). The results showed urine mono-2-ethylhexyl phthalate (MEHP) level was positively correlated with 5mdC/dG and CIMT, respectively. In logistic regression models, the odds ratios (OR) of thicker CIMT (greater than 75ᵗʰ percentile) with one unit increase in ln-MEHP level was higher when levels of 5mdC/dG were above 50%. In structural equation model, the result showed urine MEHP levels are directly associated with CIMT. Moreover, MEHP had an indirect association with CIMT through the 5mdC/dG after adjusting other confounding effects. In the current study, urine DEHP metabolite levels were positively correlated with 5mdC/dG, and CIMT. Our results showed DEHP had a direct and indirect association with CIMT through the 5mdC/dG. The finding implies that DNA methylation may mediate the association between DEHP exposures and subclinical atherosclerosis in this young population. Future effort is needed to elucidate the causal relationship between DEHP exposure, DNA methylation and CVD.

Daphnids, including the water flea Daphnia magna, can be exploited for wastewater treatment purposes, given that they are filter feeder organisms that are able to remove suspended particles from water. The presence of pollutants, such as microplastics and chemicals, might be considered stressors and modify the behaviour and survival of D. magna individuals. The impact of the cumulative pollutants that regulate the fate of living organisms has yet to be fully determined. Here we present the effect of double and triple combinations of stressors on the behaviour of D. magna. The impact of water temperature, ammonium and polystyrene microplastics on the filtration capacity and survival of D. magna is studied. Water temperatures of 15 °C, 20 °C and 25 °C, microplastic-to-food ratios of 25% and 75%, and ammonium concentrations of 10 and 30 mg N–NH₄⁺ L⁻¹ are tested after making dual and triple combinations of the parameters. A synergistic effect between water temperature and ammonium is normally observed but not in the case of the lower values of ammonium concentration and temperature. The combination of three stressors (water temperature, microplastics and ammonium) is also found to be synergistic, producing the greatest impact on D. magna filtration capacity and reducing their survival. In comparison with the effect of the two stressor conditions, the combination of the three stressors caused a reduction of between 13.1% and 91.7% in the t₅₀% time (the time required for a 50% reduction in the D. magna filtration capacity) and a reduction of between 4.8% and 54.5% in TD50 (the time for 50% mortality).