Anthropogenic pollution is considered one of the main threats to the marine environment, and there is an imperious need to assess its potential impact on ecologically and economically relevant species. This study characterises plastic ingestion and tissue levels of potentially toxic metallic elements in Nephrops norvegicus and their simultaneous levels in abiotic compartments from three locations of the Catalan coast (NW Mediterranean Sea). A multidisciplinary assessment of the health condition of N. norvegicus through condition indices, enzymatic biomarkers and histological techniques is provided, and its relationship with anthropogenic pollutant levels explored. Plastic fibres were commonly found in stomachs of N. norvegicus (85% of the individuals), with higher abundances (13 ± 21 fibres · ind⁻¹) in specimens captured close to Barcelona. The presence of long synthetic fibres in near-bottom waters, as well as the mirroring trends in abundance among locations for water and ingested plastics, suggest that uptake from water may be occurring potentially through suspension feeding. The spatial variability in the levels of metallic elements in N. norvegicus was poorly correlated to the variability in sediments. In any case, present levels in abdominal muscle are considered safe for human consumption. Levels of ingested plastics only showed significant, yet weak, correlations with glutathione S-transferase and catalase activities. However, no other health parameter analysed showed any trend potentially associated to anthropogenic pollutant levels. Neither the condition indices nor the histopathological assessment evidenced any signs of pathologic conditions affecting N. norvegicus. Thus, it was concluded that presently there is no evidence of a negative impact of the studied pollutants on the health condition of N. norvegicus in the studied grounds.

13 pages, 4 figures, 3 tables, supplementary data https://doi.org/10.1016/j.envpol.2022.120230.-- Data availability: Data will be made available on request | Anthropogenic pollution is considered one of the main threats to the marine environment, and there is an imperious need to assess its potential impact on ecologically and economically relevant species. This study characterises plastic ingestion and tissue levels of potentially toxic metallic elements in Nephrops norvegicus and their simultaneous levels in abiotic compartments from three locations of the Catalan coast (NW Mediterranean Sea). A multidisciplinary assessment of the health condition of N. norvegicus through condition indices, enzymatic biomarkers and histological techniques is provided, and its relationship with anthropogenic pollutant levels explored. Plastic fibres were commonly found in stomachs of N. norvegicus (85% of the individuals), with higher abundances (13 ± 21 fibres · ind−1) in specimens captured close to Barcelona. The presence of long synthetic fibres in near-bottom waters, as well as the mirroring trends in abundance among locations for water and ingested plastics, suggest that uptake from water may be occurring potentially through suspension feeding. The spatial variability in the levels of metallic elements in N. norvegicus was poorly correlated to the variability in sediments. In any case, present levels in abdominal muscle are considered safe for human consumption. Levels of ingested plastics only showed significant, yet weak, correlations with glutathione S-transferase and catalase activities. However, no other health parameter analysed showed any trend potentially associated to anthropogenic pollutant levels. Neither the condition indices nor the histopathological assessment evidenced any signs of pathologic conditions affecting N. norvegicus. Thus, it was concluded that presently there is no evidence of a negative impact of the studied pollutants on the health condition of N. norvegicus in the studied grounds | This study was supported by the Spanish Ministry of Science, Innovation and Universities project ‘PLASMAR’ (RTI 2018-094806-B-100) and by the Catalan Department of Agriculture, Livestock, Fisheries and Food (European Maritime and Fisheries Fund (EMFF)) project ‘SOMPESCA’ (ARP059/19/00003). [...] ECC benefited from an FPU PhD student grant from the Spanish Ministry of Science, Innovation and Universities (FPU16/03430). MG and PZ acknowledge financial support from the i-plastic project (JPI-Oceans—Grant PCI 2020-112059). JC and MS acknowledge their institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX 2019-000928-S) and CSIC Interdisciplinary Thematic Platform (PTI+) for Sustainable Plastics towards a Circular Economy+ (PTI-SusPlast+) | Peer reviewed

Sediment dissolved organic matter (DOM) in inland waters is commonly affected by environmental changes. However, knowledge about how sediment DOM responds to eutrophication and the associations between sediment DOM and bacterial communities requires further investigation. We selected a sediment core from Dianchi Lake (China) that was dated from 1864 to 2019 by the activity of radionuclides (²¹⁰Pb and ¹³⁷Cs). δ¹³CDOC changes fit well with the historical record that heavy eutrophic status in Dianchi Lake were observed since 1980s. Large amounts of dissolved organic carbon (DOC), chromophoric (CDOM) and fluorescent (FDOM) DOM accumulated at the top of the sediments during the eutrophication period (1982-present). The additional algae sources with a higher degradation rate altered the composition, aromaticity and humification of DOM. After long-term mineralization, the remaining DOM became more and more recalcitrant and kept a relatively stable level at older sediments. A co-occurrence network analysis revealed that Proteobacteria, Chloroflexi, Acidobacteriota, Bacteroidota and Desulfobacterota were the most abundant species at the phylum level and clustered into three primary modules. Different microbes shared unique preferences for niches, causing a heterogeneous bacterial distribution at different depths. We conducted Spearman's correlation and redundancy analysis (RDA) to explore potential interactions between bacterial community and sediment DOM. The richness and diversity of bacterial communities were positively related to DOM content, suggesting abundant DOM can produce more available resources for bacteria. RDA results showed some specific species might modify DOM composition and structure. This study suggests that sediment DOM properties were regulated by source transformation during eutrophication, and emphasizes the importance of microbial role on sediment biogeochemical process.

12 pages, 8 figures | Sediment dissolved organic matter (DOM) in inland waters is commonly affected by environmental changes. However, knowledge about how sediment DOM responds to eutrophication and the associations between sediment DOM and bacterial communities requires further investigation. We selected a sediment core from Dianchi Lake (China) that was dated from 1864 to 2019 by the activity of radionuclides (210Pb and 137Cs). δ13CDOC changes fit well with the historical record that heavy eutrophic status in Dianchi Lake were observed since 1980s. Large amounts of dissolved organic carbon (DOC), chromophoric (CDOM) and fluorescent (FDOM) DOM accumulated at the top of the sediments during the eutrophication period (1982-present). The additional algae sources with a higher degradation rate altered the composition, aromaticity and humification of DOM. After long-term mineralization, the remaining DOM became more and more recalcitrant and kept a relatively stable level at older sediments. A co-occurrence network analysis revealed that Proteobacteria, Chloroflexi, Acidobacteriota, Bacteroidota and Desulfobacterota were the most abundant species at the phylum level and clustered into three primary modules. Different microbes shared unique preferences for niches, causing a heterogeneous bacterial distribution at different depths. We conducted Spearman's correlation and redundancy analysis (RDA) to explore potential interactions between bacterial community and sediment DOM. The richness and diversity of bacterial communities were positively related to DOM content, suggesting abundant DOM can produce more available resources for bacteria. RDA results showed some specific species might modify DOM composition and structure. This study suggests that sediment DOM properties were regulated by source transformation during eutrophication, and emphasizes the importance of microbial role on sediment biogeochemical process | This study was financially supported by the National Natural Science Foundation of China [Grant Nos. 41773097, 41673108, and 41971286], Postgraduate Research Innovation Project of Jiangsu Province [Grant No. KYCX21_1338] and China Scholarship Council [Grant No. 202006860045] | Peer reviewed

Evidence shows that the majority of aquatic field microplastics (MPs) could be microfibers (MFs) which can be originated directly from massive sources such as textile production and shedding from garments, agricultural textiles and clothes washing. In addition, wear and tear of tyres (TRWPs) emerges as a stealthy major source of micro and nanoplastics, commonly under-sampled/detected in the field. In order to compile the current knowledge in regards to these two major MPs sources, concentrations of concern in aquatic environments, their distribution, bulk emission rates and water mitigation strategies were systematically reviewed. Most of the aquatic field studies presented MFs values above 50%. MPs concentrations varied from 0.3 to 8925 particles m⁻³ in lakes, from 0.69 to 8.7 × 10⁶ particles m⁻³ in streams and rivers, from 0.16 to 192000 particles m⁻³ estuaries, and from 0 to 4600 particles m⁻³ in the ocean. Textiles at every stage of production, use and disposal are the major source of synthetic MFs to water. Laundry estimates showed an averaged release up to 279972 tons year⁻¹ (high washing frequency) from which 123000 tons would annually flow through untreated effluents to rivers, streams, lakes or directly to the ocean. TRWPs in the aquatic environments showed concentrations up to 179 mg L⁻¹ (SPM) in runoff river sediments and up to 480 mg g⁻¹ in highway runoff sediments. Even though average TRWR emission is of 0.95 kg year⁻¹ per capita (10 nm- 500 μm) there is a general scarcity of information about their aquatic environmental levels probably due to no-availability or inadequate methods of detection. The revision of strategies to mitigate the delivering of MFs and TRWP into water streams illustrated the importance of domestic laundry retention devices, Waste Water Treatment Plants (WWTP) with at least a secondary treatment and stormwater and road-runoff collectors quality improvement devices.

Evidence shows that the majority of aquatic field microplastics (MPs) could be microfibers (MFs) which can be originated directly from massive sources such as textile production and shedding from garments, agricultural textiles and clothes washing. In addition, wear and tear of tyres (TRWPs) emerges as a stealthy major source of micro and nanoplastics, commonly under-sampled/detected in the field. In order to compile the current knowledge in regards to these two major MPs sources, concentrations of concern in aquatic environments, their distribution, bulk emission rates and water mitigation strategies were systematically reviewed. Most of the aquatic field studies presented MFs values above 50%. MPs concentrations varied from 0.3 to 8925 particles m−3 in lakes, from 0.69 to 8.7 × 106 particles m−3 in streams and rivers, from 0.16 to 192000 particles m−3 estuaries, and from 0 to 4600 particles m−3 in the ocean. Textiles at every stage of production, use and disposal are the major source of synthetic MFs to water. Laundry estimates showed an averaged release up to 279972 tons year−1 (high washing frequency) from which 123000 tons would annually flow through untreated effluents to rivers, streams, lakes or directly to the ocean. TRWPs in the aquatic environments showed concentrations up to 179 mg L−1 (SPM) in runoff river sediments and up to 480 mg g−1 in highway runoff sediments. Even though average TRWR emission is of 0.95 kg year−1 per capita (10 nm- 500 μm) there is a general scarcity of information about their aquatic environmental levels probably due to no-availability or inadequate methods of detection. The revision of strategies to mitigate the delivering of MFs and TRWP into water streams illustrated the importance of domestic laundry retention devices, Waste Water Treatment Plants (WWTP) with at least a secondary treatment and stormwater and road-runoff collectors quality improvement devices. | Fil: Arias, Andres Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina. Universidad Nacional del Sur. Departamento de Química; Argentina | Fil: Alfonso, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina | Fil: Girones, Lautaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina | Fil: Piccolo, Maria Cintia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina. Universidad Nacional del Sur. Departamento de Geografía y Turismo; Argentina | Fil: Marcovecchio, Jorge Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Argentino de Oceanografía. Universidad Nacional del Sur. Instituto Argentino de Oceanografía; Argentina. Universidad Tecnológica Nacional. Facultad Regional Bahía Blanca; Argentina

Antarctic marine ecosystems are often considered to be pristine environments, yet wildlife in the polar regions may still be exposed to high levels of environmental contaminants. Here, we measured total mercury (THg) concentrations in blood samples from adult brown skuas Stercorarius antarcticus lonnbergi (n = 82) from three breeding colonies south of the Antarctic Polar Front in the Southern Ocean (southwest Atlantic region): (i) Bahía Esperanza/Hope Bay, Antarctic Peninsula; (ii) Signy Island, South Orkney Islands; and, (iii) Bird Island, South Georgia. Blood THg concentrations increased from the Antarctic Peninsula towards the Antarctic Polar Front, such that Hg contamination was lowest at Bahía Esperanza/Hope Bay (mean ± SD, 0.95 ± 0.45 μg g⁻¹ dw), intermediate at Signy Island (3.42 ± 2.29 μg g⁻¹ dw) and highest at Bird Island (4.47 ± 1.10 μg g⁻¹ dw). Blood THg concentrations also showed a weak positive correlation with δ¹⁵N values, likely reflecting the biomagnification process. Males had higher Hg burdens than females, which may reflect deposition of Hg into eggs by females or potentially differences in their trophic ecology. These data provide important insights into intraspecific variation in contamination and the geographic transfer of Hg to seabirds in the Southern Ocean.

International audience | Antarctic marine ecosystems are often considered to be pristine environments, yet wildlife in the polar regions may still be exposed to high levels of environmental contaminants. Here, we measured total mercury (THg) concentrations in blood samples from adult brown skuas Stercorarius antarcticus lonnbergi (n = 82) from three breeding colonies south of the Antarctic Polar Front in the Southern Ocean (southwest Atlantic region): (i) Bahía Esperanza/Hope Bay, Antarctic Peninsula; (ii) Signy Island, South Orkney Islands; and, (iii) Bird Island, South Georgia. Blood THg concentrations increased from the Antarctic Peninsula towards the Antarctic Polar Front, such that Hg contamination was lowest at Bahía Esperanza/Hope Bay (mean ± SD, 0.95 ± 0.45 µg g-1 dw), intermediate at Signy Island (3.42 ± 2.29 µg g-1 dw) and highest at Bird Island (4.47 ± 1.10 µg g-1 dw). Blood THg concentrations also showed a weak positive correlation with δ15N values, likely reflecting the biomagnification process. Males had higher Hg burdens than females, which may reflect deposition of Hg into eggs by females or potentially differences in their trophic ecology. These data provide important insights into intraspecific variation in contamination and the geographic transfer of Hg to seabirds in the Southern Ocean.

A biological treatment method was tested in laboratory conditions for the removal of hydrocarbons contained in a waste disposal soil sample consisting of excavated sandy soil from a former fueling station. Two fractions of hydrocarbons were quantified by GC-FID: diesel (C₁₀–C₂₁) and lubricant oil (C₂₂–C₄₀). Meat and bone meal (MBM, 1% w/w) was used as a bio-stimulant agent for soil organisms. Cyclodextrin, an oligosaccharide produced from starch by enzymatic conversion, was also used to assess its ability to improve the bioavailability/biodegradability of hydrocarbons in the soil. Parameters such as temperature, pH, water content and aeration (O₂ availability) were monitored and optimized to favor degradation processes. Two different experimental tests were prepared: one to measure the degradation of hydrocarbons; the other to monitor the mobility of some elements in the soil and in the leachate produced by watering with tap water. Soil samples treated with MBM and cyclodextrin showed, over time, a greater removal of the more persistent hydrocarbon fraction (lubricant oil). MBM-treated soils underwent a faster hydrocarbon removal kinetic, especially in the first treatment period. However, the final hydrocarbon concentrations are comparable in all treatments, including control. Over time, the effect of cyclodextrin on hydrocarbon degradation seemed to be relevant. MBM-treated soils sequestered lead in the very first weeks. These results highlight the intrinsic capacity of soil, and its indigenous microbial communities, to degrade petroleum hydrocarbons and suggest that MBM-induced bioremediation is a promising, environmentally friendly technology which should be considered when dealing with hydrocarbon/heavy metal co-contaminated soils.

2022

International audience | Once previous industrial activity has ceased, brownfields are found in urban and suburban environments and managed in different ways ranging from being left untouched to total reconversion. These situations apply to large surface areas often impacted by residual diffuse pollution. Though significant and preventing any sensitive use, residual contamination does not necessarily require treatment. Moreover, conventional treatments show their technical and economic limits in these situations and gentle remediation options such as phytomanagement might appear more relevant to the management of those sites. Thus, these sites face up two major issues: managing moderate contamination levels and providing an alternative use of economic interest. This work proposes to assess a management strategy associating the phytoremediation of organic pollution along with the production of biomass for energy generation production. A 16-week controlled growth experiment was conducted on a soil substrate moderately impacted by multiple pollution (trace elements, mainly Zn and Pb, and hydrocarbons), by associating rhizodegradation with Medicago sativa or biomass production with Robinia pseudoacacia or Alnus incana in monocultures. The effect of a microbial inoculum amendment on the performances of these treatments was also evaluated. Results showed total hydrocarbons (TH), and to a lesser extent polycyclic aromatic hydrocarbons (PAH), concentrations decreased over time, whatever the plant cover. Good biomass production yields were achieved for both tree species in comparison with the control sample, even though R. pseudoacacia seemed to perform better. Furthermore, the quality of the biomass produced was in conformity with the thresholds set by the legislation concerning its use as a renewable energy source.

Once previous industrial activity has ceased, brownfields are found in urban and suburban environments and managed in different ways ranging from being left untouched to total reconversion. These situations apply to large surface areas often impacted by residual diffuse pollution. Though significant and preventing any sensitive use, residual contamination does not necessarily require treatment. Moreover, conventional treatments show their technical and economic limits in these situations and gentle remediation options such as phytomanagement might appear more relevant to the management of those sites. Thus, these sites face up two major issues: managing moderate contamination levels and providing an alternative use of economic interest. This work proposes to assess a management strategy associating the phytoremediation of organic pollution along with the production of biomass for energy generation production. A 16-week controlled growth experiment was conducted on a soil substrate moderately impacted by multiple pollution (trace elements, mainly Zn and Pb, and hydrocarbons), by associating rhizodegradation with Medicago sativa or biomass production with Robinia pseudoacacia or Alnus incana in monocultures. The effect of a microbial inoculum amendment on the performances of these treatments was also evaluated. Results showed total hydrocarbons (TH), and to a lesser extent polycyclic aromatic hydrocarbons (PAH), concentrations decreased over time, whatever the plant cover. Good biomass production yields were achieved for both tree species in comparison with the control sample, even though R. pseudoacacia seemed to perform better. Furthermore, the quality of the biomass produced was in conformity with the thresholds set by the legislation concerning its use as a renewable energy source.

International audience | Sustainable solutions aiming at limiting Reynoutria japonica invasion consist of frequent removal of its aerial biomass. The aims of this study were to measure the accumulation of metallic trace elements (MTE) in R. japonica, and to assess the ecotoxicological risk related to the valorization of the produced biomass. R. japonica fragmented rhizomes were regenerated in pots for 41 days on a control soil (CTL) or a moderately MTE-contaminated soil (POL, 3.6 mg Cd kg −1 DM). Growth traits were recorded, as well as MTE bioconcentration (BCF) and translocation factors (TF) from soil to plant organs. Whatever the MTE and plant organs, BCF remained below one (mean Cd-BCF for stem and leaf: 0.07 and 0.29 for CTL and POL, respectively), conversely to TF (until 2.2 for Cd and Ni in POL soil). When grown on the POL soil, R. japonica stem and leaf Cd content was close to the EU maximum regulatory limit for organic amendments or animal feed. Model simulations suggested that liver and kidney Cd concentrations would exceed the regulatory limit in food when adult cattle or sheep constantly ingest R. japonica grown on the POL soil over 200 to 800 days. The results of the present study will be useful to help managers in selecting efficient and safe solutions for the control of R. japonica invasion.

Sustainable solutions aiming at limiting Reynoutria japonica invasion consist of frequent removal of its aerial biomass. The aims of this study were to measure the accumulation of metallic trace elements (MTE) in R. japonica, and to assess the eco-toxicological risk related to the valorization of the produced biomass. R. japonica fragmented rhizomes were regenerated in pots for 41 days on a control soil (CTL) or a moderately MTE-contaminated soil (POL, 3.6 mg Cd kg⁻¹ DM). Growth traits were recorded, as well as MTE bioconcentration (BCF) and translocation factors (TF) from soil to plant organs. Whatever the MTE and plant organs, BCF remained below one (mean Cd-BCF for stem and leaf: 0.07 and 0.29 for CTL and POL, respectively), conversely to TF (until 2.2 for Cd and Ni in POL soil). When grown on the POL soil, R. japonica stem and leaf Cd content was close to the EU maximum regulatory limit for organic amendments or animal feed. Model simulations suggested that liver and kidney Cd concentrations would exceed the regulatory limit in food when adult cattle or sheep constantly ingest R. japonica grown on the POL soil over 200 to 800 days. The results of the present study will be useful to help managers in selecting efficient and safe solutions for the control of R. japonica invasion.

Microplastics are a relevant environmental concern in marine ecosystems due to their ubiquity. However, knowledge on their dispersion patterns within the ocean basin and the interaction with biota are scarce and mostly limited to surface waters. This study investigated microplastic contamination in two species of deep-sea cephalopods from the southwestern Atlantic with different ecological behaviour: the vampire squid (Vampyroteuthis infernalis) and the midwater squid (Abralia veranyi). Microplastic contaminated most of the evaluated specimens. V. infernalis showed higher levels of contamination (9.58 ± 8.25 particles individual−1; p < 0.05) than A. veranyi (2.37 ± 2.13 part. ind.−1), likely due to the feeding strategy of V. infernalis as a faecal pellets feeder. The size of extracted microplastics was inversely proportional to the depth of foraging. The microplastics were highly heterogeneous in composition (shape, colour and polymer type). Our results provide information regarding microplastic interaction with deep-sea organisms and evidence of the biological influence in the microplastic sinking mechanism.

Microplastics are a relevant environmental concern in marine ecosystems due to their ubiquity. However, knowledge on their dispersion patterns within the ocean basin and the interaction with biota are scarce and mostly limited to surface waters. This study investigated microplastic contamination in two species of deep-sea cephalopods from the southwestern Atlantic with different ecological behaviour: the vampire squid (Vampyroteuthis infernalis) and the midwater squid (Abralia veranyi). Microplastic contaminated most of the evaluated specimens. V. infernalis showed higher levels of contamination (9.58 ± 8.25 particles individual⁻¹; p < 0.05) than A. veranyi (2.37 ± 2.13 part. ind.⁻¹), likely due to the feeding strategy of V. infernalis as a faecal pellets feeder. The size of extracted microplastics was inversely proportional to the depth of foraging. The microplastics were highly heterogeneous in composition (shape, colour and polymer type). Our results provide information regarding microplastic interaction with deep-sea organisms and evidence of the biological influence in the microplastic sinking mechanism.