The responses of Dicentrarchus labrax and Liza aurata to aquatic pollution were assessed in a contaminated coastal lagoon, using both traditional and novel biomarkers combined. DNA damage, assessed by comet assay, was higher in both fish species from the contaminated sites, whereas levels of cytochrome P450 1A1 gene expression were not significantly altered. The liver histopathological analysis also revealed significant lesions in fish from contaminated sites. Alterations in ras and xpf genes were analysed and additional pollutant-responsive genes were identified. While no alterations were found in ras gene, a downregulation of xpf gene was observed in D. labrax from a contaminated site. Suppression subtractive hybridization applied to D. labrax collected at a contaminated site, revealed altered expression in genes involved in energy metabolism, immune system activity and antioxidant response. The approach and results reported herein demonstrate the utility of anchoring traditional biomarker responses alongside novel biomarker responses.

High mountains may play significant roles in the global transport of persistent organic pollutants (POPs). This work aims to investigate the levels, patterns and distribution of semi-volatile organoclorine pollutants and to improve the understanding of the long-range atmospheric transport and fate of contaminants on the Tibetan Plateau. A total of 60 fish samples were collected from eight lakes located between 2813 and 4718 m above sea level across the Plateau. Concentrations of polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs) including dichlorodiphenyltrichloroethane and its metabolites (DDTs), hexachlorocyclohexanes (HCHs) and hexachlorobenzene (HCB) were measured in fish muscle. The results showed that concentrations of DDT, HCH and HCB were comparable to or lower than those found in remote mountains of Europe, Canada and US, while PCB concentrations in fish were, on average, about 4-150 times lower on Tibet than at other mountain areas. The transport and fate of contaminants in the Plateau are significantly influenced by the unique climatological and meteorological conditions, particularly by the summer Indian monsoon and winter westerly jet stream.

[Departement_IRSTEA]Ecotechnologies [TR1_IRSTEA]TED [Axe_IRSTEA]TED-EPURE | International audience | French vertical flow constructed wetlands treating raw wastewater have been developed successfully over the last 30 years. During the last years, SINT and Epur Nature developed the approach further by introducing a deep single-stage vertical flow bed that comprises both stages into one in the aim to reduce the footprint. This process, patented by Epur Nature as Bi-filtre®, consists in two vertical stages piled-up. While classical systems in France are designed with 2m².PE-1 (1.2m² and 0.8m² on the first and second stage respectively), the Bi-filtre® has a larger total filtration area with 2.5 m²/PE (1.5m² and 1 m² on the first upper and second lower stage respectively) but a foot print reduced to 1.5 m².PE-1. To favour aeration of the system, an intermediate natural aeration system is introduced at the interface between the first and second stage. The paper will present a study on a fullscale plant (Saint Michel l'Observatoire, 175 PE, France) running at full load in which different materials (sand, pozzolana of different particle size) were tested at the second stage. The aim was to identify the best materials to use to guarantee performance and avoid clogging on this unplanted and buried stage. The study consisted in measuring hydraulic conditions (inlet/outlet flows, infiltration rates, tracer experiments) correlated with 24h composite samples assessments. The results shows that such systems, if well designed, can guarantee an outlet quality of 35 mg.L-1 in SS, 125 mg.L-1 in COD, 25 mg.L-1 in BOD and 20 mg/L in KN with a total foot print of 1.5 m².PE-1. The paper will present the design recommendations and the limits of the systems taking into account a larger experience gathered on different existing plants (e.g. Chorges Bifilter®, 2 150 P.E, France).

[Departement_IRSTEA]Ecotechnologies [TR1_IRSTEA]TED [Axe_IRSTEA]TED-EPURE | International audience | We develop an original solid respirometric method to measure the biodegradation rates of COD and ammonium at different depths of a Vertical Flow Constructed Wetland (VFCW). The method is developed for a matrix operated under unsaturated conditions. The oxygen demand is measured versus time in a gas passing through a reactor containing a mixture of an organic matrix mixed with a bulking agent (wood). For the measurement of the nitrification rate, a solution of ammonia and hydrogenocarbonates is spread on the sample. We ran 15 experiments on different matrices with variable organic matter contents (sludge, VFCW gravels and sand). Within this paper we present the overall methodology, the factors that allow improving the accuracy (number of duplicates, blank, sample volume, bulking agent volume, gas flow, etc.) and the nitrification rates that were obtained.

[Departement_IRSTEA]Ecotechnologies [TR1_IRSTEA]TED [Axe_IRSTEA]TED-EPURE | International audience | Subsurface horizontal flow constructed wetlands (HFCW) and vertical flow constructed wetlands (VFCW) have been compared regarding their efficiencies on microbiological indicators removal. Seven types of HFCWs and two types of VFCWs have been monitored for several years. Fecal coliforms, E. coli, somatic coliphages and F-specific bacteriophages were analyzed in the influent and effluents of each system. All constructed wetlands monitored were planted with Phragmites australis but have different design characteristics and operation variables. All data were statistically treated (SPSS v17) in order to analyse the effect of design and operation variables on each microbiological indicator. VFCWs were more efficient than HFCWs systems when considering removal rates per surface for all the microbiological indicators (cfu or pfu removed/m2.d). Nevertheless, considering mean removal efficiencies (in log units), results did not show statistically significant differences. The hydraulic retention time was the key parameter regarding filter disinfection capacity in both types of filters. Bacterial indicators were removed at a higher rate than viral indicators.

[Departement_IRSTEA]Ecotechnologies [TR1_IRSTEA]TED [Axe_IRSTEA]TED-EPURE | International audience | Oxygen renewal, as a prominent phenomenon for aerobic bacteria activity, deeply impacts VFCW treatment efficiency. We introduce MIGRA, a new model able to simulate oxygen transfer in VFCW. It is based on a realistic two-phase flow module, that allows us to describe various loading sequences, and a transport module. The multi-component transport module is able to deal with convection/diffusion phenomena, inter-phase (air-water) mass exchange, and first-order kinetics. It is validated with analytical solutions and data from an experimental VFCW (column). We show the benefits of a two-phase flow modeling using a comparison with HYDRUS.

[Departement_IRSTEA]Ecotechnologies [TR1_IRSTEA]TED [Axe_IRSTEA]TED-EPURE | International audience | This paper presents the key elements that emerged from a 3-year study on 4 campsites. Each of them has its own Vertical-Flow Constructed Wetlands (VFCW) for on-site wastewater treatment. Each year, the pollution discharge peaked in the 5 to 6-week summer period but was zero for a 6-month period. The natural-concentrate wastewater is collected into a short sewer network. Hydraulic load and campground occupancy rate were tightly correlated. The effluent has a characteristically high nitrogen concentration due to the lower greywater-producing activity. These quantitative and qualitative elements were used to define the camper equivalent' as a daily pollutant load of about 100 L water, 90 gCOD, 35 gBOD5, 40 gSS, 11 gKN and 1.4 gPt. During the 5-6 weeks of peak visitor traffic, the full first stage receives an organic load of 200 gCOD.m-2.d-1 and 25 gKN.m-2.d-1, which is 2 to 3.5-fold higher than the loads conventionally applied in VFCW systems in France. Despite these heavy loads, COD removal remains constant (at 91%) over the season. During the summer peak, nitrification drops sharply but KN removal rate holds steady near 60%. This study demonstrates that VFCWs can robustly handle massive load increases. The paper concludes that total system size can be downscaled to 0.75 m²/camper. This design allows to reach minimum yields of 75% KN, provided that the system is built and run to state-of-the-art rules. | Cet article présente les éléments clés issus d'une étude de 3 ans sur 4 campings qui traitent leurs eaux usées de manière individuelle par des Filtres Plantés de Roseaux à écoulement vertical (FPRV) aux dimensionnements différents. La charge hydraulique et le taux d'occupation du camping sont étroitement corrélés. L'émission est nulle 6 mois par an et la pointe estivale dure 5 à 6 semaines. Les eaux usées, collectées par un réseau court sont naturellement concentrées. L'effluent présente notamment une très forte concentration en azote du fait d'une activité ménagère réduite. Ces éléments qualitatifs et quantitatifs permettent de définir l' « équivalent campeur » par une charge de pollution journalière d'environ 100 L d'eau, 90 g de DCO, 35 g de DBO5, 40 g de MES, 11 g de NK et 1,4 g de Pt. Au cours des 5-6 semaines de fréquentation maximale, la totalité du 1er étage reçoit une charge organique d'environ 200 gDCO.m-².j-1 et 25 gNK.m-².j-1, soit 2 à 3,5 fois plus que les charges classiques appliquées en FPRV en France. En dépit de ces fortes charges, l'élimination de la DCO reste constante (91 %) au cours de la saison. Lors du pic de fréquentation estival, la nitrification décroît fortement mais le rendement global se maintient à environ 60 %. La robustesse des FPRV face à une augmentation massive des charges appliquées est démontrée. L'article conclut par un dimensionnement réduit de 0.75 m2/campeur au total. Ce dimensionnement permet d'atteindre un rendement minimum de 75 % du NK, correspondant à la nitrification, sous réserve que les règles de l'art de construction et d'exploitation soient strictement respectées.

International audience

International audience

In Portugal, Atlantic codfish (Gadus morhua) is usually consumed after dry salting; this process is carried out by mixing deboned codfish with food-grade marine salt followed by stacking in a tank for 6 days. Along the salting process, codfish incorporates salt as well as it is partially dried by the release of water - up to 22 % (w/w). Currently, this wastewater is treated as a residue not being further valorized. However, the presence of a significant amount of valuable compounds in this rest, such as amino acids and proteins, may allow for valorization opportunities not yet explored. The present work focuses on the identification of the nitrogen-containing compounds present in such wastewater. Total nitrogen (WSN), trichloroacetic acid-soluble nitrogen (TCASN) and phosphotungstic acid-soluble nitrogen (PTASN) were evaluated by the micro-Kjeldahl method; Biuret method was used for total protein determinations and SDS-Page was performed for protein molecular weight screening. The results revealed an increase of WSN, TCASN and PTASN with time, with corresponding values of 3.17 g/L (WSN), 1.62 g/L (TCASN) and 1.16 g/L (PTASN) by the end of the process; the evolution of WSN versus the released water was approximately constant during the salting process, as well as the ratios of TCASN/WSN and PTASN/WSN with values of 51.25 and 36.55 % (w/w) at equilibrium, respectively.