Aim of this study was to determine by Ion Chromatography ions (Na+, Ca++, Mg++, NH4+, Cl−, Br−, SO42−, NO3−, PO43−) in honeys (honeydew and floral nectar honeys) from different Italian Regions and from countries of the Western Balkan area. The compositional data were processed by multivariate analysis (PCA and HCA). Arboreal honeydew honeys from the Western Balkans had higher concentrations (from two to three times) of some environmental pollutants (Br−, SO42− and PO43− contents), due to industrial and agricultural activities, than those from Italian regions. The cationic profiles were very similar in both groups. Multivariate analysis indicated a clear difference between nectar honeys and arboreal/honeydew honeys (recognition of the botanical origin). These findings point to the potential of ionic constituents of honey as indicators of environmental pollution, botanical origin and authenticity.

The acute and chronic effects of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) on the rotifer Brachionus calyciflorus (B. calyciflorus) were investigated at the organismal and the population level. The acute toxicity of PFOS to rotifers was approximately 2.5-fold greater than that of PFOA. From 0.25 to 2.0 mg L−1, PFOS exhibited higher toxicity than PFOA on the F0-generation of B. calyciflorus, including effects on body size, juvenile periods, net reproductive rate, and generation time. Both PFOS and PFOA exposure induced a smaller egg size in B. calyciflorus, suggesting that these risks can be transferred from mother to offspring. The 28-day population growth studies showed that PFOS and PFOA reduced the population density and increased the mictic ratio. Our results demonstrated that both PFOS and PFOA had adverse effects on B. calyciflorus, not only at the individual level but also at the population level.

A number of studies have shown the potential of in vitro assays to predict contaminant in vivo relative bioavailability in order to refine human health exposure assessment. Although the term ‘validated’ has been used to describe the goodness of fit between in vivo and in vitro observations, its misuse has arisen from semantic considerations in addition to the lack of defined criteria for establishing performance validation. While several internal validation methods may be utilised, performance validation should preferably focus on assessing the agreement of model predictions with a set of data which are independent of those used to construct the model. In order to achieve robust validated predictive models, a number of parameters (e.g. size of data set, source of independent soils, contaminant concentration range, animal model, relative bioavailability endpoint) need to be considered in addition to defined criteria for establishing performance validation which are currently lacking.

Air pollution is a widespread health problem associated with respiratory symptoms. Continuous exposure monitoring was performed to estimate alveolar and tracheobronchial dose, measured as deposited surface area, for 103 children and to evaluate the long-term effects of exposure to airborne particles through spirometry, skin prick tests and measurement of exhaled nitric oxide (eNO). The mean daily alveolar deposited surface area dose received by children was 1.35 × 103 mm2. The lowest and highest particle number concentrations were found during sleeping and eating time. A significant negative association was found between changes in pulmonary function tests and individual dose estimates. Significant differences were found for asthmatics, children with allergic rhinitis and sensitive to allergens compared to healthy subjects for eNO. Variation is a child's activity over time appeared to have a strong impact on respiratory outcomes, which indicates that personal monitoring is vital for assessing the expected health effects of exposure to particles.

Air pollution has many negative effects on the natural environment, from changes in plant growth patterns to loss of ecosystem function. This study uses citizen science to investigate national-scale patterns in the distribution and abundance of selected lichen species on tree trunks and branches, and to relate these to air pollution and climate. Volunteers collected data for nine lichen indicators on 19,334 deciduous trees. Submitted data provided information on species-level patterns, and were used to derive composite lichen indices. Multiple linear regression and ANCOVA were used to model the relationships between lichen response variables on Quercus spp. and pollution, climate and location. The study demonstrated significant relationships between patterns in indicator lichens and levels of N- and S-containing pollutants on trunks and twigs. The derived lichen indices show great potential as a tool to provide information on local, site-specific levels of air quality.

Recently, several researchers have attempted to address Deepwater Horizon incident environmental fate and effects issues using laboratory testing and extrapolation procedures that are not fully reliable measures for environmental assessments. The 2013 Rico-Martínez et al. publication utilized laboratory testing approaches that severely limit our ability to reliably extrapolate such results to meaningful real-world assessments.The authors did not adopt key methodological elements of oil and dispersed oil toxicity standards. Further, they drew real-world conclusions from static exposure tests without reporting actual exposure concentrations. Without this information, it is not possible to compare their results to other research or real spill events that measured and reported exposure concentrations.The 1990s' Chemical Response to Oil Spills: Ecological Effects Research Forum program was established to standardize and conduct exposure characterization in oil and dispersed oil aquatic toxicity testing (Aurand and Coelho, 2005). This commentary raises awareness regarding the necessity of standardized test protocols.

While a number of studies have addressed the environmental presence and behavior of the Dechlorane Plus (DP) flame retardant, there is still a dearth of information in terrestrial ecosystems. The present study revealed that median ∑DP (including anti- and syn-DP isomers) concentrations ranged from 10 to 810 ng/g lipid weight in muscle and liver tissues of six terrestrial raptor species collected in 2004–2006 from Beijing, China. Some concentrations rival the greatest DP burdens ever reported in global wildlife. Significant, positive correlations were observed between fanti (concentration ratio of anti-isomer to ∑DP) and ∑DP concentrations in the Eurasian sparrowhawk (Accipiter nisus) tissues. These results suggested that the DP burdens could be substantially driven by the accumulation of the anti-isomer in terrestrial birds. The tissue-specific accumulation of DP further suggested that factors (e.g., hepatic binding enzymes) other than lipid solubility could be important in determining tissue deposition of DP.

The National Descriptive Model for Mercury in Fish (NDMMF) was applied to a Canada – wide dataset of fish mercury (Hg) concentrations to derive a common indicator of dietary methylmercury (MeHg) exposure (HgPREY) to piscivorous fish and wildlife. The NDMMF provided unbiased parameter estimates and strong spatial biases in prediction error were not apparent. Prediction error was comparatively higher when sample sizes were small and events with high Hg concentrations. Estimates of HgPREY from 1936 locations between 1990 and 2010 averaged 0.09 ± 0.07 μg g−1 (wet wt) and increased from west to east in a manner consistent with independent measures of MeHg exposure in piscivorous wildlife and conceptual models of aquatic ecosystem sensitivity to Hg methylation and bioaccumulation. The HgPREY dataset offers an approach to evaluate the risk of MeHg exposure to piscivorous fish and wildlife on a continental scale.

Using synchrotron- and electron microscope-based X-ray microanalyses, the distribution and speciation of Zn and Pb were examined in situ in two earthworm species (Dendrodrilus rubidus and Lumbricus rubellus) living in heavily-polluted soils. Main findings: (i) Zn spectra in ingested soil and in tissues more closely resembled Zn3(PO4)2 than ZnS; (ii) Zn speciation in tissues gave a best fit for Zn to the inner shell of 4 oxygens at 1.94 Å (or nitrogens at 1.96 Å); (iii) the best fit for Pb in tissue was with a shell of oxygens at 2.18 Å and a shell of sulphurs at 2.67 Å; (iv) a component of the Zn and much of the Pb detectable in gut contents was co-distributed with S; (v) Zn and Pb display ‘soft’ acid affinities in soil, but ‘hard’ acid affinities in tissue. This is the first metal characterisation study conducted on an invertebrate quench-frozen in the field.

The dissolution of ZnO nanoparticles (nano-ZnO) plays an important role in the toxicity of nano-ZnO to the aquatic organisms. The effects of water chemistry such as pH, ionic components, and dissolved organic matter (DOM) on the dissolution of nano-ZnO and its toxicity to Escherichia coli (E. coli) were investigated in synthetic and natural water samples. The results showed that the toxicity of nano-ZnO to E. coli depended on not only free Zn2+ but also the coexisting cations which could reduce the toxicity of Zn2+. Increasing solution pH, HPO42−, and DOM reduced the concentration of free Zn2+ released from nano-ZnO, and thus lowered the toxicity of nano-ZnO. In addition, both Ca2+ and Mg2+ dramatically reduced the toxicity of Zn2+ to E. coli. These results highlight the importance of water chemistry on the toxicity evaluation of nano-ZnO in natural waters.