Gasification has gained advantage as an effective way to dispose domestic solid waste in mountainous rural of China. However, its toxic emissions such as PCDD/Fs and heavy metals, as well as their potential environmental risks, were not well studied in engineering application. In this study, an updraft fixed bed gasifier was investigated by field sampling analysis. Results showed that low toxic emissions (dust, SO₂, NOx, HCl, CO, H₂S, NH₃, PCDD/Fs and heavy metals) in the flue gas were achieved when the rural solid waste was used as feedstock. The mass distribution of heavy metals showed that 94.00% of Pb, 80.45% of Cu, 78.00% of Cd, 77.31% of Cr, and 76.25% of As were remained in residual, whereas 86.58% of Hg was found in flue gas. The content of PCDD/Fs in the flue gas was 0.103 ngI-TEQ·Nm⁻³, and the total emission factor of PCDD/Fs from the gasifier was 50.04 μgI-TEQ·t-waste⁻¹, among which only 0.04 μgI-TEQ·t-waste⁻¹ was found in the flue gas. The total output of PCDD/Fs was1.89 times as high as input, indicting the updraft fixed bed gasifier increased emission of PCDD/Fs during the treatment domestic solid waste. In addition, the distribution characteristics of PCDD/Fs congeners reflected that PCDD/Fs was mainly generated in the gasification process rather than the stage of flue gas cleaning, suggesting the importance to effectively control the generation of PCDD/Fs within the gasifier chamber in order to obtain a low PCDD/Fs emission level.

Over the past decades, an increasing need in renewable resources has progressively appeared. This trend concerns not only fossil fuels but also mineral resources. Wastewater and sewage sludge contain significant concentrations in phosphate and can be considered as a fertilizer source of the utmost importance. In wastewater treatment plants, the biological uptake of phosphate is performed by a specific microbiota: the phosphate-accumulating organisms. These microorganisms are recovered in sewage sludge. Here, we aimed to investigate the occurrence of phosphate accumulators in four wastewater treatment plants. A 16S metagenetic analysis identified the main bacterial phyla extracted from the aerobic treatment: α-Proteobacteria, β-Proteobacteria, and Sphingobacteria. An enrichment stage was performed to stimulate the specific growth of phosphate-accumulating bacteria in an acetate medium. An analysis of metabolic activities of sulfur and phosphorus highlighted strong modifications related to phosphorus and much less distinguishable effects with sulfur. A solid acetate medium containing 5-Br-4-Cl-3-indolyl phosphate was used to select potential phosphate-accumulating bacteria from the enriched consortia. The positive strains have been found to belong in the genera Acinetobacter, Corynebacterium, and Pseudomonas. Finally, electron microscopy was applied to the strains and allowed to confirm the presence of polyphosphate granules. Some of these bacteria contained granules the size of which exceeded 100 nm.

Municipal solid waste incineration (MSWI) fly ash has been classified as hazardous waste and needs treatment in an environmentally safe manner. Mechanochemical (MC) treatment is such a detoxification method, since it destroys dioxins and solidifies heavy metals. Milling, however, also introduces supplemental metals (Fe, Ni, Cr, Mn…), following wear of both steel balls and housing. Milling moreover reduces the particle size of fly ash and disperses catalytic metal, potentially rising the reactivity of fly ash to form and destroy ‘dioxins’, i.e. polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD + PCDF or PCDD/F). To test this issue, model fly ash (MFA) samples were composed by mixing of silica, sodium chloride, and activated carbon, and doped with CuCl₂. Then, these samples were first finely milled without any additives for 0 h (original sample), 1 h and 8 h, and the effect of milling time (and hence particle size) was investigated on the formation of polycyclic aromatic hydrocarbons (PAHs), and of polychlorinated phenols (CP), benzenes (CBz), biphenyls (PCB) and dioxins (PCDD + PCDF) during de novo tests at 300 °C for 1 h, thus simulating the conditions prevailing in the post-combustion zone of an incinerator, where dioxins are formed and destroyed. These compounds are all characterized by their rate of generation (ng/g MFA) and their signature, i.e. internal distribution over congeners as a means of gathering mechanistic indications. PAH and CBz total yield did not decrease in MC treated MFA with milling time, while total pentachlorophenol (PeCP), PCB and PCDD/F yield decreased up to 86, 94 and 97%, respectively. International Toxic Equivalents (I-TEQ) concentration decreased more than 90%, while degree of chlorination varied inconsistently for PCB and PCDD/F, and average congener patterns of PCDD/F do not vary considerably with milling time for both gas and solid phase.

Human adenoviruses (HAdVs) are DNA viruses found in recreational water, such as water parks and swimming pools. Human adenovirus 41 (HAdV-41) is the most common serotype detected and is a leading cause of acute diarrheal disease. The focus of this study is to determine the prevalence of HAdVs in hot springs. Of 57 samples collected from four different geological sites, 16 samples have shown evidence of HAdVs (28.1%). HAdV-41 and porcine adenovirus 5 (PAdV-5) were the two types isolated, with a greater frequency of HAdV-41, which in other settings has been associated with acute diarrhea. The highest occurrence was found in private hot tubs/Yuya (37.5%), followed by an outlet of hot springs (30.8%); public pools and foot pools shared the same detection rate of 21.4% (3/14). However, there was no evidence supporting a link between water quality indicators and HAdV detection rate. From a phylogenic analysis and BLAST against the NCBI database, it was concluded that HAdV-41 obtained from hot spring areas are closely related to global environmental genotypes.

Performance and microbial community composition were evaluated in a two-phase anaerobic and aerobic system treating sulfate-rich cellulosic ethanol wastewater (CEW). The system was operated at five different chemical oxygen demand (COD)/SO₄ ²⁻ ratios (63.8, 26.3, 17.8, 13.7, and 10.7). Stable performance was obtained for total COD removal efficiency (94.5%), sulfate removal (89.3%), and methane production rate (11.5 L/day) at an organic loading rate of 32.4 kg COD/(m³·day). The acidogenic reactor made a positive contribution to net VFAs production (2318.1 mg/L) and sulfate removal (60.9%). Acidogenic bacteria (Megasphaera, Parabacteroides, unclassified Ruminococcaceae spp., and Prevotella) and sulfate-reducing bacteria (Butyrivibrio, Megasphaera) were rich in the acidogenic reactor. In the methanogenic reactor, high diversity of microorganisms corresponded with a COD removal contribution of 83.2%. Moreover, methanogens (Methanosaeta) were predominant, suggesting that these organisms played an important role in the acetotrophic methanogenesis pathway. The dominant aerobic bacteria (Truepera) appeared to have been responsible for the COD removal of the SBR. These results indicate that dividing the sulfate reduction process could effectively minimize sulfide toxicity, which is important for the successful operation of system treating sulfate-rich CEW.

To accurately evaluate the environmental toxicity of silver nanowires (AgNWs), it is necessary to characterize how the cytotoxicity of these nanomaterials is affected by the route of exposure. However, few studies have addressed the exposure route or mechanism of toxicity of nanomaterials, particularly of nanowires, in living organisms. In this study, we therefore analyzed the main exposure route of AgNWs in vitro, using earthworms (Eisenia andrei) as a model system, via flow cytometry. We subsequently examined the in vivo toxicity of AgNWs to earthworms in soil. These tests revealed that intracellular esterase activity was correlated with adsorption of the nanowires to the surfaces of coelomocytes in vitro, and that in vivo cytotoxicity resulted mainly from oral, rather than dermal, exposure to the nanowires. Overall, shorter AgNWs (10 μm) were more toxic than longer AgNWs (20 μm). To our knowledge, this study is the first report regarding the ecotoxicity of nanowires to earthworms in soil. Our findings provide important information to help assess the risk of toxic AgNW contamination of soil ecosystems.

This study aims to investigate whether root system morphology is involved in the interspecific variations in Cd accumulation in herbaceous plants. Biomasses, root morphology, and Cd accumulation of 18 herbaceous species were determined under 0, 2, and 10 mg kg⁻¹ Cd conditions. Significant variations were found in biomass production, root system morphology, and Cd accumulation among the 18 species. Cd concentrations in the shoot had negative correlations with the biomass of roots and shoots in the 2 mg kg⁻¹ Cd treatment. Total amounts of Cd in plants showed positive correlations with the biomass of roots and shoots, total root lengths, root surface areas, root volumes, and proportions of the fine roots (diameter <0.2 mm). Percentages of Cd in shoots were positively related to specific root lengths, root surface areas, and plant biomasses but negatively correlated with proportions of roots in the 0.6–0.8-mm diameter class. High-biomass species (rapeseed, Indian mustard, and four-o’clock) have high Cd uptake capacity due to their large root system. Longer and thinner roots might contribute to higher capacity for transferring Cd from roots to shoots, while coarse roots (i.e., diameter of 0.6–0.8 mm) could retain more Cd in the tissues and, consequently, reduce Cd transfer from roots to shoots.

Concentrations of persistent organic pollutants (POPs) were quantified in river water and sediment, as well as in the liver and muscle tissues of Cyprinus carpio that were sampled four times in a year at three stations in the Büyük Menderes River (BMR). Potential biomarkers of possible cellular molecular damage, namely lipid peroxidation (LPO) degradation products, protein carbonyls (PCO) and DNA repair product 8-hydroxy-2′-deoxyguanosine (8-OHdG), were analysed. All the targeted pollutants were measurable both in biotic and abiotic samples. Interestingly, the results suggested that there was recent organochlorine pesticide (OCP) input into the river water in the first two sampling periods in all stations in contrast to prohibition, while input of polychlorinated biphenyls (PCBs) and polybrominated diphenylethers (PBDEs) was not detected. Liver POP concentrations were higher than in muscle, as expected, and were found to decrease from the first to the fourth sampling period in all stations, except PBDEs. Levels of LPO degradation products in the liver and in muscle tissues decreased from the first to the fourth sampling period. This suggests that these markers reflect the lipid damage in respective tissues due to the tissue burden of targeted POPs. Protein carbonyls were the highest in the first sampling period, followed by a dramatic decrease in the second, and then a gradual increase towards the fourth sampling period in all stations. 8-OHdG levels were lower in Sarayköy station in the first sampling period. Among the measured biomarkers, only several LPO degradation products were significantly correlated with OCPs and PCBs in liver tissue.

Toxicity data of substances to higher plants is needed for the purpose of risk assessment, site evaluation, phytoremediation, and plant protection. However, the results from the most common phytotoxicity tests, like the OECD algae and Lemna test, are not necessarily valid for higher terrestrial plants. The willow tree toxicity test uses inhibition of transpiration (aside of growth and water use efficiency) of willow cuttings grown in spiked solutions or soils as end point to quantify toxicity. This overview presents results from 60 studies including 24 new unpublished experiments for 56 different chemicals or substrates. Highest toxicity (EC₅₀ < 1 mg/L) was observed from exposure to heavy metals like copper and cadmium. Also, organotins and free cyanide showed very high toxicity. The toxic effect of chlorophenols on willows was comparable to that on duck weed (Lemna) and green algae, while volatile compounds like chlorinated solvents or benzene, toluene, ethylbenzene, and xylene had less effect on trees than on these aquatic plants, due to volatilization from leaves and test media. In particular low (g/L range) toxicity was observed for tested nanomaterials. Effects of pharmaceuticals (typically weak acids or bases) depended strongly of the solution pH. Like for algae, baseline toxicity was observed for willows, which is related to the water solubility of the compounds, with absolute chemical activity ranging from 0.01 to 0.1, but with several exceptions. We conclude that the willow tree toxicity test is a robust method for relating uptake, accumulation, and metabolism of substances to the toxicity to trees.

The citric acid fermentation broth was prepared and it was employed to washing remediation of heavy metal-polluted soil. A well-defined washing effect was obtained, the removal percentages using citric acid fermentation broth are that 48.2% for Pb, 30.6% for Cu, 43.7% for Cr, and 58.4% for Cd and higher than that using citric acid solution. The kinetics of heavy metals desorption can be described by the double constant equation and Elovich equation and is a heterogeneous diffusion process. The speciation analysis shows that the citric acid fermentation broth can effectively reduce bioavailability and environmental risk of heavy metals. Spectroscopy characteristics analysis suggests that the washing method has only a small effect on the mineral composition and does not destroy the framework of soil system. Therefore, the citric acid fermentation broth is a promising washing agent and possesses a potential practical application value in the field of remediation of soils with a good washing performance.