Endocrine disrupting chemicals (EDCs) are common pollutants in coastal waters. To investigate the estrogen risk of EDCs in the coastal areas of Japan, the Japanese common goby, which is a commonly observed species in these waters, was used as the target fish. Plasma 17β-estradiol (E₂) and vitellogenin (VTG) levels were analyzed and the gonads of fish collected from the Taira River (northern Nagasaki, reference site), Nagasaki Port, and two sites in Tokyo Bay were observed. Abnormal levels (>150 ng/mL, p < 0.05) of plasma VTG and high levels of plasma E₂ were detected in the fish from Nagasaki Port and Tokyo Bay, whereas the levels of both were low in the fish from the Taira River. The target EDCs, including natural estrogen [estrone (E₁), and E₂] and alkylphenols [4-t-octylphenol (4-t-OP), 4-nonylphenol (4-NP), and bisphenol-A (BPA)] in water samples were quantified using gas chromatography tandem mass spectrometry (GC/MS/MS), respectively. It was observed that the E₂-equivalent (EEQ) in Nagasaki Port and Tokyo Bay, which was calculated from the actual EDC measurement value, were almost 20- and 150-fold higher, respectively, than that at the reference site (Taira River, 0.021 ng/L). The EEQs mostly comprised natural estrogen in the sampling sites, although there was some influence of alkylphenols. There was an association between the EEQ and the E₂ in environmental water, suggesting a high estrogen risk in Japan coastal waters. Moreover, the results indicated that abnormal VTG synthesis was induced by environmental estrogen (EE) pollution in Nagasaki Port and Tokyo Bay.

The effects of the continuous accumulation of Zinc (Zn) on the fate of antibiotic resistance genes (ARGs) in constructed wetland-microbial fuel cells (CW-MFCs) remain unclear. In this study, the impacts of Zn addition and a circuit mode on antibiotic removal, occurrence of ARGs, the bacterial community, and bacterial functions were investigated in three groups of CW-MFCs. The results showed that continuous Zn exposure enriched the target ARGs during the initial stage, while excessive Zn accumulation decreased antibiotic removal and the abundance of ARGs. A principal component analysis demonstrated that ARGs and the bacterial community distribution characteristics were significantly impacted by the mass accumulation of antibiotics and Zn, as well as the circuit mode. A redundancy analysis, partial least squares path modeling, and Procrustes analysis revealed that the accumulation of antibiotics and Zn, the composition of the bacterial community, the circuit mode, and the abundance of intI associated with horizontal gene transfer jointly contributed to the distributions of ARGs in the electrodes and effluent. Moreover, continuous exposure to Zn decreased the bacterial diversity and changed the composition and function of the bacterial community predicted using PICRUSt tool. The co-occurrence of ARGs, their potential hosts and bacterial functions were further revealed using a network analysis. A variation partition analysis also showed that the accumulation of target pollutants and the circuit mode had a significant impact on the bacterial community composition and functions. Therefore, the interaction among ARGs, the bacterial community, bacterial functions, and pollutant accumulations in the CW-MFC was complex. This study provides useful implications for the application of CW-MFCs for the treatment of wastewater contaminated with antibiotics and heavy metals.

Due to extensive mining and industrial activities, arsenic (As) and antimony (Sb) contaminations are becoming a global environmental concern. Both As and Sb are toxic and carcinogenic metalloids from the group 15 in the periodic table. Since As and Sb share many similar geochemical properties, it is often assumed that they exert similar environmental pressure on the native microbial communities. This hypothesis, however, still requires further confirmation. In the current study, a systematic comparison of microbial responses to As and Sb contamination were conducted. The results suggested that regular geochemical parameters, such as pH, nitrate, and TOC, were the driving forces for shaping the microbial community. In correspondence, two heavily contaminated groups showed similar microbial community compositions and the same microbial populations were enriched. The interactions between the contaminant fractions (As and Sb related fractions) and the individual OTUs, however, suggested the different and more diverse impacts of As comparing to Sb fractions, with more taxa significantly impacted by As species comparing to Sb species. The identification of the keystone taxa in the heavily contaminated samples revealed a group of microbial populations that could survive in both As and Sb heavily contaminated conditions and may providing critical environmental services to the community. Further investigation of these key microbial populations may provide valuable insights on employing these microorganisms for remediation applications.

Samples from two reservoirs with eutrophication problems, located in Pontevedra and Ourense (Northwestern Spain), were cultured, along with a third crop from a reservoir with no problems detected in Ourense (Northwestern Spain). The samples were grown under the same conditions (with an average temperature of 21 ± 2 °C, and a 3000 lux light intensity) in triplicate, and their growth, absorbance and pH were studied. High correlation values were obtained for pH and cellular growth (R² ≥ 95%). The water from Salas showed the greatest microalgal growth (0.15 × 10⁶ cells/ml to 31.70 × 10⁶ cells/ml of Microcystis sp. for the last day of culturing) and the greatest increase in pH (5.72–9.02). In all the cultures studied here, the main species that reproduced was Microcystis sp., which can produce neurotoxins and hepatotoxins. In addition, water samples were cultured with sediments of their own reservoir and with others to observe their evolution. The sediments studied in this case were rich in biotites, which can lead phosphate to be a limiting factor for phytoplankton due to the formation and sedimentation of insoluble salts of ferric phosphate. In crops grown with sediments from the Salas reservoir, actinobacteria developed which can inhibit microalgal growth. The study of the growth of cyanobacteria and possible methods of inhibiting them directly concerns the quality of water and its ecosystems, avoiding pollution and impact on ecosystems.

Problems with PM₂.₅ pollution in the Pearl River Delta (PRD) have been significantly reduced since the Chinese government released a series of emission control policies including the strengthened controls in the 13ᵗʰ Five-Year Plan. This study assessed the efficacy of emission control measures using the Community Multiscale Air Quality (CMAQ) model to provide data-driven support to government decision making, which is becoming increasingly important. This study aimed to quantitatively evaluate the integrated results of proposed policies for controlling PM₂.₅ concentrations. Accordingly, the regional 2015 emission inventory was modified with recently released government data for the PRD, and scenarios for four dynamical emission-reduction policies (S1–S4) were explored. The results show that all four proposed control measures can help to reduce PM₂.₅ concentrations throughout Hong Kong (HK), Macao, and the PRD economic zone (PRD EZ) by 2020. In all cases, reductions in PM₂.₅ concentrations were larger over PRD EZ than over HK. For HK, the predicted annual concentrations of PM₂.₅ were less than 20 μg/m³ for S1–S3 and less than 15 μg/m³ for S4. For Macao, the predicted annual concentrations of PM₂.₅ were less than 25 μg/m³ for S1 and less than 15 μg/m³ up to S3. Regionally, HK had the lowest PM₂.₅ levels, and the area around Foshan had the highest. Controlling the sources of air pollution (i.e., industry, transport, power production, and other sources) within PRD can get most of the PRD EZ region to below 35 μg/m³. Similar national air quality management efforts could reduce PM₂.₅ levels to less than 25 μg/m³ in the PRD EZ and less than 15 μg/m³ in HK. Control measures in S1 led to significant improvement in Shenzhen and HK, but the S3 option brought the greatest improvement for PRD EZ and Macao. The S4 policy option led to substantial reductions, particularly for HK.

Organophosphate flame retardants (OPFRs) are widespread in the aquatic environment, but the effects of these chemicals on reproductive toxicity are far from clear. In this study, sperm quality in adult male Chinese rare minnows after exposure to tris-(2-butoxyethyl) phosphate (TBOEP), tris-(1,3-dichloro-2-propyl) phosphate (TDCIPP), and triphenyl phosphate (TPHP) was investigated. No obvious change in sperm concentration and vitality was observed after treatments, whereas significant changes in sperm velocity and morphology were found following all treatments (P < 0.05). Moreover, OPFR exposure significantly increased the apoptosis ratios in testis cells. Analysis of the transcriptomic data revealed that Na⁺/K⁺ ATPase (NKA) related genes were significantly downregulated, and the NKA enzyme activities after all treatments were significantly inhibited (P < 0.05). However, no obvious change in hormone levels in the groups exposed to TBOEP and TDCIPP was observed. These findings indicate that the OPFR-induced reduction of sperm quality might be due to the effects of OPFRs on NKA enzyme instead of changes in hormone levels.

Bisphenol A (BPA) is a widely produced chemical that is mainly used as raw material for manufacturing plastic products. It is an endocrine disruptor and causes irreversible damage to the human body. Bisphenol S (BPS), an alternative to BPA, has low dose effects on toxicology and genotoxicity. Herein, we constructed a highly porous crystalline covalent organic framework (COF, CTpPa-2)-modified glassy carbon electrode (GCE) for the electrochemical sensing of BPA and BPS. The electrochemical properties of the CTpPa-2/GCE were characterized using galvanostatic charge-discharge, cyclic voltammetry and differential pulse voltammetry. The CTpPa-2/GCE exhibited remarkable electrocatalytic activity, and the electrochemical responses for BPA and BPS were found to be linear in the concentration ranges of 0.1–50 μM and 0.5–50 μM with detection limits of 0.02 μM and 0.09 μM (S/N = 3), respectively. Moreover, the fabricated sensor was utilized to determine BPA and BPS in bottle samples with recoveries of 87.0%–92.2% and migration rates of 13.2%–28.0%.

Exposure to particulate matters (PM) is recognized as an important risk factor for cardiovascular disease. A change in cardiac autonomic function is one postulated mechanism leading to PM related cardiovascular events. This study therefore evaluated the associations of short-term exposure to PM and heart rate variability (HRV) parameters, which can reflect the cardiac autonomic function.Four electronic databases were searched for controlled studies of rodents published prior to December 2018. A systematic review and meta-analysis was conducted. Effect sizes were calculated for five main HRV parameters, including standard deviation of normal-to-normal intervals (SDNN), square root of mean squared differences between successive normal-to-normal intervals (rMSSD), low frequency (LF), high frequency (HF), and the ratio of LF and HF (LF/HF).The review included 23 studies with 401 animals. Short-term exposure to PM by instillation yielded statistically significant effects on SDNN (Standardized Mean Difference [SMD] = −1.11, 95% Confidence Intervals [CI] = −2.22 to −0.01, P = 0.05), LF (SMD = −1.19, 95% CI = −1.99 to −0.40, P = 0.003) and LF/HF (SMD = −1.05, 95% CI = −2.03 to −0.07, P = 0.04). Short-term exposure to PM by inhalation only yielded statistically significant effect on LF/HF (SMD = −0.83, 95% CI = −1.39 to −0.27, P = 0.004). There was no evidence that animal model and exposure frequency influenced the relationship of PM and HRV.Short-term exposure to PM can decrease HRV of rodents, affecting cardiac autonomic function. Exposure methods can influence the relationships of PM and HRV parameters. Further studies should focus on the effects of long-term PM exposure, on human beings, and potential influential factors of PM-HRV associations.

Wastewater treatment plants (WWTPs) play important roles in water purification but are also important source of aerosols. However, the relationship between aerosol characteristics and wastewater treatment process remains poorly understood. In this study, aerosols were collected over a 24-month period from a WWTP using a modified anaerobic-anoxic-oxic process. The aerated tank (AerT) was characterized by the highest respiratory fraction (RF) concentrations (861–1525 CFU/m³) and proportions (50.76%–65.96%) of aerosol particles. Fourteen core potential pathogens and 15 toxic metal(loid)s were identified in aerosols. Mycobacterium was the genus that aerosolized most easily in fine grid, pre-anoxic tank, and AerT. High wastewater treatment efficiency may increase the emission of RF and core potential pathogens. The median size of activated sludge, richness of core potential pathogens in wastewater, and total suspended particulates were the most influential factors directly related to the RF proportions, core community of potential pathogens, and composition of toxic metal(loid)s in WWTP aerosols, respectively. Relative humidity, temperature, input and removal of biochemical oxygen demand, dissolved oxygen, and mixed liquor suspended solids could also directly or indirectly affect the aerosol characteristics. This study enhances the mechanistic understanding of linking aerosol characteristics to treatment processes and has important implications for targeted manipulation.

This paper aims to identify the atmospheric boundary layer turbulence structure and its effect on severe foggy haze events frequently occurring in Northern China. We use data collected from a ground eddy covariance system, meteorology tower, and a PM₂.₅ collector in Baoding, China during December 2016. The data shows that 73.5% of PM₂.₅ concentration is greater than 100 μg m⁻³ with a maximum of 522 μg m⁻³. Analyses on vertical turbulence spectrum also reveal that 1) during the pollution period, lower wind can suppress large-scale turbulence eddies, which are more likely inhomogeneous, breaking into small-scale eddies, and 2) the air pollutant scattering effect for radiation could decrease the air temperature near the ground and generate weak vertical turbulence during the daytime. At night, air pollutants suppress the land surface cooling and decrease the air temperature difference as well as the vertical turbulence intensity difference. The vertical turbulence impact analysis reveals that the percentage of large-scale turbulence eddies can also change the atmospheric vertical mixing capacity. During the daytime, the air pollution evolution is controlled by the wind speed and vertical turbulence intensity. While at night, the vertical turbulence is weak and the atmospheric vertical mixing capacity is mainly controlled by the large-scale eddies’ percentage. The increased number of large-scale turbulence eddies led by low wind at night could increase the vertical mixing of air pollutants and decrease its concentration near the ground.