Cadmium (Cd)-polluted soils were collected from wasteland, farmland, and slopeland surrounding a lead–zinc mine in Yunnan Province, Southwest China. Maize plants (the host) were inoculated with arbuscular mycorrhizal fungi (AMF) in a dual-compartment cultivation system that included mycorrhizal and hyphal compartments as part of an AMF inoculation treatment and root and soil compartments as part of a the non-inoculation treatment. The effects of AMF on maize biomass and Cd uptake, soil aggregate composition, and Cd concentration in the interflow within two soil layers (0–20 and 20–40 cm) as well as the Cd leaching from these three Cd-polluted soils under simulated heavy rainfall (40 and 80 mm/h) were investigated. The results demonstrated that AMF led to increased maize biomass and Cd uptake. There were greater contents of total glomalin-related soil protein (T-GRSP) and >2.0 mm aggregates and lower Cd concentrations in the interflow and lower dissolved Cd leaching in the mycorrhizal and hyphal compartments than in the soil compartment. A two-way analysis of variance revealed that AMF significantly increased the contents of T-GRSP and >2.0 mm aggregates and reduced both Cd concentrations in the interflow and dissolved Cd leaching. Moreover, AMF interacted extensively with the roots and affected soil aggregate composition and Cd concentrations in the interflow. Under 40 mm/h of rainfall, the contents of T-GRSP and >2.0 mm aggregates were significantly negatively correlated with both Cd concentrations in the interflow and dissolved Cd leaching. In addition, the Cd concentrations in the interflow were significantly positively correlated with the amount of dissolved Cd leaching. Therefore, both AMF-reduced Cd concentrations in the interflow and Cd leaching from Cd-polluted soils were closely related to increased T-GRSP contents and macroaggregate proportion in the soils.

Jicama peroxidase (JP) was covalently immobilized onto functionalized multi-walled carbon nanotube (MWCNT) Buckypaper/Polyvinyl alcohol (BP/PVA) membrane and employed for degradation of methylene blue dye. The parameters of the isotherm and kinetic models are estimating using ant colony optimization (ACO), which do not meddle the non-linearity form of the respective models. The proposed inverse modelling through ACO optimization was implemented, and the parameters were evaluated to minimize the non-linear error functions. The adsorption of MB dye onto JP-immobilized BP/PVA membrane follows Freundlich isotherm model (R² = 0.99) and the pseudo 1st order or 2nd kinetic model (R² = 0.980 & 0.968 respectively). The model predictions from the parameters estimated by ACO resulted values close the experimental values, thus inferring that this approach captured the inherent characteristics of MB adsorption. Moreover, the thermodynamic studies indicated that the adsorption was favourable, spontaneous, and exothermic in nature. The comprehensive structural analyses have confirmed the successful binding of peroxidase onto BP/PVA membrane, as well as the effective MB dye removal using immobilized JP membrane. Compared to BP/PVA membrane, the reusability test revealed that JP-immobilized BP/PVA membrane has better dye removal performances as it can retain 64% of its dye removal efficiency even after eight consecutive cycles. Therefore, the experimental results along with modelling results demonstrated that JP-immobilized BP/PVA membrane is expected to bring notable impacts for the development of effective green and sustainable wastewater treatment technologies.

The non-magnetic capping materials are difficult to be recycled from the water bodies after their application, leading to the increase in the cost of the sediment remediation. To address this issue, a capping material, i.e., magnetic lanthanum/iron-modified bentonite (M-LaFeBT) was prepared by loading lanthanum onto a magnetic iron-modified bentonite (M-FeBT) and used to control the internal phosphorus (P) loading in this study. To determine the capping efficiency and mechanism of M-LaFeBT, the impact of M-LaFeBT and M-FeBT capping on the mobilization of P in sediments was investigated, and the stabilization of P bound by the M-LaFeBT and M-FeBT capping layers was evaluated. Results showed that M-LaFeBT possessed good magnetic property with a saturated magnetization of 14.9 emu/g, and exhibited good phosphate adsorption ability with a maximum monolayer sorption capacity (QMAX) of 14.3 mg P/g at pH 7. Moreover, M-LaFeBT capping tremendously reduced the concentration of soluble reactive P (SR-P) in the overlying water (OL-water), and the reduction efficiencies were 94.7%–97.4%. Furthermore, M-LaFeBT capping significantly decreased the concentration of SR-P in the pore water and DGT (diffusive gradient in thin films)-labile P in the profile of OL-water and sediment. Additionally, most of P bound by the M-LaFeBT capping layer (approximately 77%) was stable under natural pH and reducing conditions. The phosphate adsorption ability for M-LaFeBT was much higher than that for M-FeBT, and the QMAX value for the former was 4.86 times higher than that for the latter. M-LaFeBT capping gave rise to a higher reduction of DGT-labile concentration in the profile of OL-water and sediment than M-FeBT capping. The P adsorbed by the M-LaFeBT capping layer was more stable than that by the M-FeBT capping layer. Results of this study demonstrate that M-LaFeBT is promising for utilization as an active capping material to intercept sedimentary P release into OL-water.

Trace elements (TEs) in the insoluble particles of surface snow are less affected by melting processes and can be used as environmental proxies to reveal natural and anthropogenic emissions. Here the first comprehensive study of the 16 TEs (Al, As, Ba, Bi, Cr, Cu, Fe, Mn, Ni, Pb, Sn, Sr, Ti, U, V, and Zn) in insoluble particles (>0.45 μm) from surface snow samples collected at Urumqi Glacier No. 1 (UG1), Eastern Tien Shan, China, from February 2008 to January 2010 were presented. Results show that concentrations of most insoluble particulate TEs (TEs ᵢₙₛₒₗ) in the snow were higher in summer while lower in winter, due to the increasing particle inputs and melting processes. The abundances of As, Cr, Cu, Ni, Pb, and Zn in some samples were higher than those in surrounding urban soils, which might due to these TEs have further anthropogenic input beyond the already contaminated re-suspended urban soil particles and TEs were mainly enriched in particles with small grain size. Based on enrichment factor (EF) and principal component analysis (PCA), our results suggest that eight TEs (Al, Fe, Ti, Ba, Mn, Sr, U, and V) mainly came from mineral dust, while the remaining eight TEs (As, Bi, Cr, Cu, Ni, Pb, Sn, and Zn) were affected by coal combustion, mining and smelting of non-ferrous metals, traffic emissions, and the steel industry. The Hybrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT) model suggests that pollutants might originate from Xinjiang province, Kazakhstan, and Kyrgyzstan. Moreover, UG1 received more significant inputs of particle-bound pollutants in summer than in winter due to the stronger convection and the prevailing valley wind that transports pollutants from the city of Urumqi.

The fluoride-sensitive indica rice cultivar, IR-64 was subjected to NaF-treatment for 25 days, following which RNA-Seq analysis identified significant up and down regulation of 1,303 and 93 transcripts respectively. Gene ontology (GO) enrichment analysis classified transcripts into groups related to ‘cellular part’, ‘membrane’, ‘catalytic activity’, ‘transporter activity’, ‘binding’, ‘metabolic processes’ and ‘cellular processes’. Analysis of differentially expressed genes (DEGs) revealed fluoride-mediated suppression of abscisic acid (ABA) biosynthesis and signaling. Instead, the gibberellin-dependent pathway and signaling via ABA-independent transcription factors (TFs) was activated. Comparative profiling of selected DEGs in IR-64 and fluoride-tolerant variety, Khitish revealed significant cytoskeletal and nucleosomal remodelling, accompanied with escalated levels of autophagy in stressed IR-64 (unlike that in stressed Khitish). Genes associated with ion, solute and xenobiotic transport were strongly up regulated in stressed IR-64, indicating potential fluoride entry through these channels. On the contrary, genes associated with xenobiotic mobility were suppressed in the tolerant cultivar, which restricted bioaccumulation and translocation of fluoride. Pairwise expression profile analysis between stressed IR-64 and Khitish, supported by extensive statistical modelling predicted that fluoride susceptibility was associated with high expression of genes like amino acid transporter, ABC transporter2, CLCd, MFS monosaccharide transporter, SulfT2.1 and PotT2 while fluoride tolerance with high expression of Sweet11.

The toxicity of mercury (Hg), is generally known, and around 90% of Hg exist as methylmercury (CH₃Hg⁺) in marine organism. Mercury concentrates in sharks and whales, which are at the top of the food chain as predators to cephalopods. The concentrations of Hg in liver and muscle of blue shark, caught in Sagami Bay, and in digestive gland and mantles of Todarodes pacificus, Sepia madokai, and Uroteuthis edulis caught in East China Sea were measured and analyzed. The Hg concentrations in the sharks, squids, and cuttlefishes determined in this study were almost same as those in the other sea regions. In addition, the Hg concentration in the blue shark was higher in the muscle than in the liver. In S. madokai and U. edulis, Hg accumulated in the digestive gland but not in the mantle. Although the Hg concentration in the digestive gland of T. pacificus is lower than those of S. madkai and U edulis, Hg concentration in the mantle is critically higher. More than 90% of Hg is present as CH₃Hg⁺ in muscle of blue shark and mantle of T. pacificus. This feature is explained due to amino acids with the thiol groups and chain genes in the muscle of blue shark as well as in the mantle of T. pacificus. Myosin in the mantle of T. pacificus and blue shark enhances the stability of CH₃Hg⁺. The amount of Hg in the digestive gland of T. pacificus could be too large to store; thus, Hg is released to the mantle, whereas the nutrients in the digestive gland of T. pacificus are supplied to other tissues. It is considered that the muscle fiber of T. pacificus is strong; therefore, large amounts of myosin levels may be present in T. pacificus than in S. madokai and U. edulis.

In this study, the distribution profiles, emission characteristics, and health risks associated with 43 volatile and semi-volatile organic compounds, including 15 phenols, 18 polycyclic aromatic hydrocarbons (PAHs), 6 BTEX, and 4 other compounds, were determined in the wastewater treatment plant (WWTP) of a coking factory (plant C) and the succeeding final WWTP (central WWTP). Total phenols with a concentration of 361,000 μg L⁻¹ were the predominant compounds in the influent wastewater of plant C, whereas PAHs were the major compounds in the final effluents of both coking WWTPs (84.4 μg L⁻¹ and 30.7 μg L⁻¹, respectively). The biological treatment process in plant C removed the majority of volatile organic pollutants (94.1%–99.9%). A mass balance analysis for plant C showed that biodegradation was the main removal pathway for all the target compounds (56.6%–99.9%) except BTEX, chlorinated phenols, and high molecular weight (MW) PAHs. Chlorinated phenols and high MW PAHs were mainly removed via sorption to activated sludge (51.8%–73.2% and 60.2%–75.9%, respectively). Air stripping and volatilization were the dominant mechanisms for removing the BTEX compounds (59.8%–73.8%). The total emission rates of the detected volatile pollutants from plant C and the central WWTP were 1,640 g d⁻¹ and 784 g d⁻¹, respectively. Benzene from the equalization basins of plant C and the central WWTP corresponded to the highest inhalation carcinogenic risks (1.4 × 10⁻³ and 3.2 × 10⁻⁴, respectively), which exceeded the acceptable level for human health (1 × 10⁻⁶) recommended by the United States Environmental Protection Agency. The results showed that BaP exhibited the highest inhalation non-cancer risk, with a hazard index ratio of 70 and 30 for plant C and the central WWTP, respectively. Moreover, the excess sludge generated during wastewater treatment should also be carefully handled because it adsorbed abundant PAHs and chlorinated phenols at coking plant C (58,000 μg g⁻¹ and 3,500 μg g⁻¹) and the central WWTP (622 μg g⁻¹ and 54 μg g⁻¹).

Polycyclic aromatic hydrocarbons (PAHs) pollution has become a worldwide environmental problem, and the spread of PAHs can cause carcinogenicity, mutagenicity, and toxicity to humans. However, the transfer and accumulation of PAHs in crop leaves has not been clearly understood. In this study, we first reported that the environmental temperature could induce phenanthrene transfer and accumulation in hydrocultured wheat, corn and soybean leaves via vacuum-infiltration-centrifugation method. Phenanthrene accumulation rises significantly (p < 0.05) in the first 8 h and reaches the maximum accumulation rate at the 4th h. Then the accumulation turns stable in both apoplast and sympalst of wheat, soybean and corn leaves. Temperature is positively correlated with phenanthrene accumulation in apoplast and sympalst of soybean and corn leaves, and phenanthrene accumulation increases under lower temperature in apoplast and sympalst of wheat leaves. Temperature also displays a positive correlation with the phenanthrene accumulation under gradient phenanthrene treatments in both apoplast and sympalst. In addition, the wheat, corn and soybean leaves have the same phenanthrene accumulation pathways and symplast pathway is major for phenanthrene accumulation with the contribution over 55% in total phenanthrene accumulation. Meanwhile, based on the Elovich equation, the symplast and apoplast processes of phenanthrene accumulation are endothermic. In the end, our findings will offer a new understanding for phenanthrene transfer and accumulation pathway in plant leaves and put forward a new biological reference of PAHs transfer in environmental science.

Several studies have investigated the effects of nano- and microplastics on daphnids as key freshwater species. However, while information is abundant on the acute toxicity of plastic beads, little is known regarding the multigenerational effects of irregular microplastics. In addition, a comparison of microplastics to naturally occurring particles is missing. Therefore, we investigated the effects of irregular, secondary polystyrene microplastics (<63 μm) and kaolin as natural reference particle on the survival, reproduction, and growth of Daphnia magna over four generations under food-limited conditions. Additionally, we tested the sensitivity of the neonates in each generation to a reference compound as a proxy for offspring fitness. Exposure to high concentrations of microplastics (10,000 and 2000 particles mL⁻¹) reduced daphnid survival, resulting in extinction within one and four generations, respectively. Microplastics also affected reproduction and growth. Importantly, an exposure to kaolin at similar concentrations did not induce negative effects. The sensitivity of neonates to potassium dichromate was not affected by maternal exposure to particles. Taken together, our study demonstrates that irregular PS particles are more toxic than natural kaolin in daphnids exposed over multiple generations under food limitation. Thus, our work builds towards more realistic exposure scenarios needed to better understand the impacts of microplastics on zooplankton.

The widespread detection of 17β-estradiol (E2) in the environment has become an emerging concern worldwide due to its endocrine disrupting effects. This work focuses on the aerobic and anaerobic biodegradations of E2 in various sedimentary environments with different availabilities of electron acceptors, including O₂, NO₃⁻, Fe³⁺, SO₄²⁻, or HCO₃⁻. The highest removal efficiency (98.9%) and shortest degradation half-life of E2 (t₁/₂ = 5.0 d) were achieved under aerobic condition, followed by nitrate-reducing, ferric-reducing, sulfate-reducing and methanogenic conditions. We propose four different degradation pathways of E2 based on the metabolites identified under various redox conditions. Although most of E2 was effectively removed under aerobic condition, the potential environmental risk still needs to be considered due to the residual estrogenic activity induced by estrone (E1) formation. The endocrine-disrupting activities, as indicated by estradiol equivalent (EEQ) values, were related to E2 degradation rate and metabolite formation. We further analyzed the succession of bacterial community compositions and functions using Illumina HiSeq sequencing and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt). The findings herein evidenced that bacterial community compositions and metabolic functions associated with different redox conditions impact the biodegradation of E2 and its endocrine-disrupting activity. This knowledge will be useful in predicting the environmental fates of estrogenic hormones in various sedimentary environments and aid in establishing appropriate strategies for eliminating potential environmental risks.